VPS35 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35
Entrez Gene ID	105400394
Full Name	VPS35, retromer complex component
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011574089.1	XP_011572391.1	vacuolar protein sorting-associated protein 35 isoform X2
XM_011574088.1	XP_011572390.1	vacuolar protein sorting-associated protein 35 isoform X1

Pathways from BioSystems
Gene Ontology

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq14047	XM_011574088.1 Latest version!	Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAq14048	XM_011574089.1 Latest version!	Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35 ( XM_011574088.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572390.1 Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq14047
Clone ID Related Accession (Same CDS sequence)	XM_011574088.1
Accession Version	XM_011574088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	vacuolar protein sorting-associated protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCCAA CAACACAGCA ATCTCCTCAG GATGAACAGG AAAAACTCCT GGATGAAGCC 
ATTCAGGCTG TGAAGGTGCA GTCTTTCCAG ATGAAGAGAT GCTTGGACAA AAACAAGCTC 
ATGGATGCTT TGAAACATGC TTCCAACATG CTTGGTGAGC TGCGGACTTC TATGTTATCT 
CCAAAGAGCT ATTATGAGCT CTACATGGCA ATTTCTGATG AGCTACACTA CTTGGAAGTC 
TACCTGACAG ATGAATTTGC CAAAGGAAGG AAAGTGGCAG ACCTTTATGA GCTAGTACAG 
TACGCTGGAA ATATTATTCC AAGACTGTAT CTCCTGATAA CAGTAGGAGT TGTCTATGTC 
AAGTCATTTC CACAGTCCAG GAAAGATATT TTGAAAGACC TTGTGGAGAT GTGCCGTGGA 
GTACAGCATC CTCTTAGAGG CCTCTTTCTT AGAAACTATC TCCTGCAGTG TACCAGGAAT 
ATCTTACCAG ATGAAGGCGA GCAAGCAGAT GAAGAAACCA CTGGAGACAT CAGTGATTCA 
ATGGATTTTG TGCTGCTGAA CTTTGCTGAG ATGAACAAAC TTTGGGTGCG AATGCAACAC 
CAGGGCCATA GTCGGGACAG AGAGAAAAGG GAGCGAGAAA GGCAGGAACT GAGAATCCTA 
GTAGGAACAA ACCTGGTTCG CCTCAGTCAG TTGGAAGGTG TTAATGTGGA GCGATATAAG 
CAGATTGTTC TGCCCGGAAT ATTGGAGCAA GTTGTAAACT GTAGAGATGC TTTAGCTCAG 
GAGTACCTCA TGGAGTGCAT CATACAGGTT TTCCCAGATG AATTTCACCT CCAAACCTTG 
AACCCATTTC TCAGAGCCTG TGCTGAGCTG CACCAAAATG TGAATGTGAA AAATATAATT 
ATTGCTTTAA TTGACAGGTT AGCTTTATTT GCACATCGTG AAGATGGACC TGGTATCCCA 
GCAGATATCA AACTGTTTGA CATCTTTTCA CAGCAGGTTG CTACTGTAAT ACAGTCTCGC 
CAGGACATGC CCTCAGAGGA TGTTGTATCT TTGCAAGTTT CTCTCATTAA CTTGGCTATG 
AAATGCTATC CAGACCGTGT TGACTATGTT GATAAGGTGT TGGAGACAAC TGTGGAAATA 
TTCAATAAAC TTAATCTGGA ACATATTGCA ACAAGCAGTG CTGTTTCAAA GGAGCTGACT 
AGACTTTTGA AAATCCCTAT TGATACTTAC AACAATATCC TGACAGTTCT GAAACTAAAA 
CATTTTCACC CACTCTTTGA ATACTTTGAT TACGAGTCCA GGAAGAGCAT GAGTTGTTAC 
GTGCTTAGCA ATGTATTGGA TTATAACACA GAAATTGTGT CCCAAGAACA GGTTGATGCT 
ATCATGAATT TGGTATCCAC ACTGATCCAG GATCAACCGG ATCAGCCTGC TGAAGATCCT 
GACCCTGAGG ATTTTGCTGA TGAGCAGAGT CTTGTGGGAA GATTTATTCA TCTTTTGCGT 
TCAGATGACC CTGACCAACA GTATCTGATC CTAAATACTG CCCGGAAACA CTTTGGTGCA 
GGTGGGAACC AACGTATTCG TTTTACACTG CCACCACTGG TTTTTGCTGC ATACCAGCTT 
GCTTTTCGCT ACAAGGAGAA CTCCAAAGTG GATGACAAAT GGGAAAAGAA GTGCCAGAAG 
ATCTTCTCGT TTGCTCATCA GACCATCAGT GCTCTGATCA AAGCAGAACT GGCAGAGCTG 
CCTCTTCGTC TCTTCCTACA AGGAGCACTG GCTGCAGGAG AGATTGGCTT TGAAAATCAT 
GAAACTGTGG CCTATGAATT CATGTCCCAG GCTTTCTCTC TATATGAAGA TGAAATCAGT 
GATTCAAAAG CACAGCTGGC TGCAATCACC TTGATAATTG GGACATTTGA GAGGATGAAG 
TGCTTCAGTG AGGAGAACCA TGAGCCTTTG AGGACTCAGT GTGCGCTGGC AGCCTCTAAG 
CTCCTTAAGA AGCCAGACCA ATGCCGGGCT GTGAGCACTT GTGCCCATCT GTTTTGGTCT 
GGCCGAAATA CTGACAAGAA TGGAGAGGAG CTTCATGGAG GAAAAAGAGT GATGGAATGC 
CTAAAGAAGG CTCTGAAGAT AGCAAATCAG TGCATGGATC CTTCTCTGCA AGTCCAACTT 
TTCATAGAAA TTCTGAATAG ATATATCTAT TTTTATGAAA AGGAAAATGA GGCGGTGACA 
ATTCAGGTTT TGAATCAGCT TATACAGAAG ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT 
ACTGAAGAAA CAGAACAGAT TAACAAACAC TTTCACAACA CACTGGAGCA CTTGCGTCTG 
AGGAGGGAAT CACCAGAATC TGAGGGGCCA ATTTATGAAG GTCTTGTTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572390.1
CDS	30..2423
Translation	MTPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEQADEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPIDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPDQPAEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVL

Target ORF information:

RefSeq Version	XM_011574088.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574088.1

ORF Insert Sequence:

ATGACGCCAA CAACACAGCA ATCTCCTCAG GATGAACAGG AAAAACTCCT GGATGAAGCC 
ATTCAGGCTG TGAAGGTGCA GTCTTTCCAG ATGAAGAGAT GCTTGGACAA AAACAAGCTC 
ATGGATGCTT TGAAACATGC TTCCAACATG CTTGGTGAGC TGCGGACTTC TATGTTATCT 
CCAAAGAGCT ATTATGAGCT CTACATGGCA ATTTCTGATG AGCTACACTA CTTGGAAGTC 
TACCTGACAG ATGAATTTGC CAAAGGAAGG AAAGTGGCAG ACCTTTATGA GCTAGTACAG 
TACGCTGGAA ATATTATTCC AAGACTGTAT CTCCTGATAA CAGTAGGAGT TGTCTATGTC 
AAGTCATTTC CACAGTCCAG GAAAGATATT TTGAAAGACC TTGTGGAGAT GTGCCGTGGA 
GTACAGCATC CTCTTAGAGG CCTCTTTCTT AGAAACTATC TCCTGCAGTG TACCAGGAAT 
ATCTTACCAG ATGAAGGCGA GCAAGCAGAT GAAGAAACCA CTGGAGACAT CAGTGATTCA 
ATGGATTTTG TGCTGCTGAA CTTTGCTGAG ATGAACAAAC TTTGGGTGCG AATGCAACAC 
CAGGGCCATA GTCGGGACAG AGAGAAAAGG GAGCGAGAAA GGCAGGAACT GAGAATCCTA 
GTAGGAACAA ACCTGGTTCG CCTCAGTCAG TTGGAAGGTG TTAATGTGGA GCGATATAAG 
CAGATTGTTC TGCCCGGAAT ATTGGAGCAA GTTGTAAACT GTAGAGATGC TTTAGCTCAG 
GAGTACCTCA TGGAGTGCAT CATACAGGTT TTCCCAGATG AATTTCACCT CCAAACCTTG 
AACCCATTTC TCAGAGCCTG TGCTGAGCTG CACCAAAATG TGAATGTGAA AAATATAATT 
ATTGCTTTAA TTGACAGGTT AGCTTTATTT GCACATCGTG AAGATGGACC TGGTATCCCA 
GCAGATATCA AACTGTTTGA CATCTTTTCA CAGCAGGTTG CTACTGTAAT ACAGTCTCGC 
CAGGACATGC CCTCAGAGGA TGTTGTATCT TTGCAAGTTT CTCTCATTAA CTTGGCTATG 
AAATGCTATC CAGACCGTGT TGACTATGTT GATAAGGTGT TGGAGACAAC TGTGGAAATA 
TTCAATAAAC TTAATCTGGA ACATATTGCA ACAAGCAGTG CTGTTTCAAA GGAGCTGACT 
AGACTTTTGA AAATCCCTAT TGATACTTAC AACAATATCC TGACAGTTCT GAAACTAAAA 
CATTTTCACC CACTCTTTGA ATACTTTGAT TACGAGTCCA GGAAGAGCAT GAGTTGTTAC 
GTGCTTAGCA ATGTATTGGA TTATAACACA GAAATTGTGT CCCAAGAACA GGTTGATGCT 
ATCATGAATT TGGTATCCAC ACTGATCCAG GATCAACCGG ATCAGCCTGC TGAAGATCCT 
GACCCTGAGG ATTTTGCTGA TGAGCAGAGT CTTGTGGGAA GATTTATTCA TCTTTTGCGT 
TCAGATGACC CTGACCAACA GTATCTGATC CTAAATACTG CCCGGAAACA CTTTGGTGCA 
GGTGGGAACC AACGTATTCG TTTTACACTG CCACCACTGG TTTTTGCTGC ATACCAGCTT 
GCTTTTCGCT ACAAGGAGAA CTCCAAAGTG GATGACAAAT GGGAAAAGAA GTGCCAGAAG 
ATCTTCTCGT TTGCTCATCA GACCATCAGT GCTCTGATCA AAGCAGAACT GGCAGAGCTG 
CCTCTTCGTC TCTTCCTACA AGGAGCACTG GCTGCAGGAG AGATTGGCTT TGAAAATCAT 
GAAACTGTGG CCTATGAATT CATGTCCCAG GCTTTCTCTC TATATGAAGA TGAAATCAGT 
GATTCAAAAG CACAGCTGGC TGCAATCACC TTGATAATTG GGACATTTGA GAGGATGAAG 
TGCTTCAGTG AGGAGAACCA TGAGCCTTTG AGGACTCAGT GTGCGCTGGC AGCCTCTAAG 
CTCCTTAAGA AGCCAGACCA ATGCCGGGCT GTGAGCACTT GTGCCCATCT GTTTTGGTCT 
GGCCGAAATA CTGACAAGAA TGGAGAGGAG CTTCATGGAG GAAAAAGAGT GATGGAATGC 
CTAAAGAAGG CTCTGAAGAT AGCAAATCAG TGCATGGATC CTTCTCTGCA AGTCCAACTT 
TTCATAGAAA TTCTGAATAG ATATATCTAT TTTTATGAAA AGGAAAATGA GGCGGTGACA 
ATTCAGGTTT TGAATCAGCT TATACAGAAG ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT 
ACTGAAGAAA CAGAACAGAT TAACAAACAC TTTCACAACA CACTGGAGCA CTTGCGTCTG 
AGGAGGGAAT CACCAGAATC TGAGGGGCCA ATTTATGAAG GTCTTGTTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( XM_011574089.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572391.1 Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq14048
Clone ID Related Accession (Same CDS sequence)	XM_011574089.1
Accession Version	XM_011574089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	vacuolar protein sorting-associated protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACAA CACAGCAATC TCCTCAGGAT GAACAGGAAA AACTCCTGGA TGAAGCCATT 
CAGGCTGTGA AGGTGCAGTC TTTCCAGATG AAGAGATGCT TGGACAAAAA CAAGCTCATG 
GATGCTTTGA AACATGCTTC CAACATGCTT GGTGAGCTGC GGACTTCTAT GTTATCTCCA 
AAGAGCTATT ATGAGCTCTA CATGGCAATT TCTGATGAGC TACACTACTT GGAAGTCTAC 
CTGACAGATG AATTTGCCAA AGGAAGGAAA GTGGCAGACC TTTATGAGCT AGTACAGTAC 
GCTGGAAATA TTATTCCAAG ACTGTATCTC CTGATAACAG TAGGAGTTGT CTATGTCAAG 
TCATTTCCAC AGTCCAGGAA AGATATTTTG AAAGACCTTG TGGAGATGTG CCGTGGAGTA 
CAGCATCCTC TTAGAGGCCT CTTTCTTAGA AACTATCTCC TGCAGTGTAC CAGGAATATC 
TTACCAGATG AAGGCGAGCA AGCAGATGAA GAAACCACTG GAGACATCAG TGATTCAATG 
GATTTTGTGC TGCTGAACTT TGCTGAGATG AACAAACTTT GGGTGCGAAT GCAACACCAG 
GGCCATAGTC GGGACAGAGA GAAAAGGGAG CGAGAAAGGC AGGAACTGAG AATCCTAGTA 
GGAACAAACC TGGTTCGCCT CAGTCAGTTG GAAGGTGTTA ATGTGGAGCG ATATAAGCAG 
ATTGTTCTGC CCGGAATATT GGAGCAAGTT GTAAACTGTA GAGATGCTTT AGCTCAGGAG 
TACCTCATGG AGTGCATCAT ACAGGTTTTC CCAGATGAAT TTCACCTCCA AACCTTGAAC 
CCATTTCTCA GAGCCTGTGC TGAGCTGCAC CAAAATGTGA ATGTGAAAAA TATAATTATT 
GCTTTAATTG ACAGGTTAGC TTTATTTGCA CATCGTGAAG ATGGACCTGG TATCCCAGCA 
GATATCAAAC TGTTTGACAT CTTTTCACAG CAGGTTGCTA CTGTAATACA GTCTCGCCAG 
GACATGCCCT CAGAGGATGT TGTATCTTTG CAAGTTTCTC TCATTAACTT GGCTATGAAA 
TGCTATCCAG ACCGTGTTGA CTATGTTGAT AAGGTGTTGG AGACAACTGT GGAAATATTC 
AATAAACTTA ATCTGGAACA TATTGCAACA AGCAGTGCTG TTTCAAAGGA GCTGACTAGA 
CTTTTGAAAA TCCCTATTGA TACTTACAAC AATATCCTGA CAGTTCTGAA ACTAAAACAT 
TTTCACCCAC TCTTTGAATA CTTTGATTAC GAGTCCAGGA AGAGCATGAG TTGTTACGTG 
CTTAGCAATG TATTGGATTA TAACACAGAA ATTGTGTCCC AAGAACAGGT TGATGCTATC 
ATGAATTTGG TATCCACACT GATCCAGGAT CAACCGGATC AGCCTGCTGA AGATCCTGAC 
CCTGAGGATT TTGCTGATGA GCAGAGTCTT GTGGGAAGAT TTATTCATCT TTTGCGTTCA 
GATGACCCTG ACCAACAGTA TCTGATCCTA AATACTGCCC GGAAACACTT TGGTGCAGGT 
GGGAACCAAC GTATTCGTTT TACACTGCCA CCACTGGTTT TTGCTGCATA CCAGCTTGCT 
TTTCGCTACA AGGAGAACTC CAAAGTGGAT GACAAATGGG AAAAGAAGTG CCAGAAGATC 
TTCTCGTTTG CTCATCAGAC CATCAGTGCT CTGATCAAAG CAGAACTGGC AGAGCTGCCT 
CTTCGTCTCT TCCTACAAGG AGCACTGGCT GCAGGAGAGA TTGGCTTTGA AAATCATGAA 
ACTGTGGCCT ATGAATTCAT GTCCCAGGCT TTCTCTCTAT ATGAAGATGA AATCAGTGAT 
TCAAAAGCAC AGCTGGCTGC AATCACCTTG ATAATTGGGA CATTTGAGAG GATGAAGTGC 
TTCAGTGAGG AGAACCATGA GCCTTTGAGG ACTCAGTGTG CGCTGGCAGC CTCTAAGCTC 
CTTAAGAAGC CAGACCAATG CCGGGCTGTG AGCACTTGTG CCCATCTGTT TTGGTCTGGC 
CGAAATACTG ACAAGAATGG AGAGGAGCTT CATGGAGGAA AAAGAGTGAT GGAATGCCTA 
AAGAAGGCTC TGAAGATAGC AAATCAGTGC ATGGATCCTT CTCTGCAAGT CCAACTTTTC 
ATAGAAATTC TGAATAGATA TATCTATTTT TATGAAAAGG AAAATGAGGC GGTGACAATT 
CAGGTTTTGA ATCAGCTTAT ACAGAAGATA AGAGAAGATC TCCCAAACCT TGAGTCTACT 
GAAGAAACAG AACAGATTAA CAAACACTTT CACAACACAC TGGAGCACTT GCGTCTGAGG 
AGGGAATCAC CAGAATCTGA GGGGCCAATT TATGAAGGTC TTGTTCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572391.1
CDS	74..2464
Translation	MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEQADEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPIDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPDQPAEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVL

Target ORF information:

RefSeq Version	XM_011574089.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574089.1

ORF Insert Sequence:

ATGCCAACAA CACAGCAATC TCCTCAGGAT GAACAGGAAA AACTCCTGGA TGAAGCCATT 
CAGGCTGTGA AGGTGCAGTC TTTCCAGATG AAGAGATGCT TGGACAAAAA CAAGCTCATG 
GATGCTTTGA AACATGCTTC CAACATGCTT GGTGAGCTGC GGACTTCTAT GTTATCTCCA 
AAGAGCTATT ATGAGCTCTA CATGGCAATT TCTGATGAGC TACACTACTT GGAAGTCTAC 
CTGACAGATG AATTTGCCAA AGGAAGGAAA GTGGCAGACC TTTATGAGCT AGTACAGTAC 
GCTGGAAATA TTATTCCAAG ACTGTATCTC CTGATAACAG TAGGAGTTGT CTATGTCAAG 
TCATTTCCAC AGTCCAGGAA AGATATTTTG AAAGACCTTG TGGAGATGTG CCGTGGAGTA 
CAGCATCCTC TTAGAGGCCT CTTTCTTAGA AACTATCTCC TGCAGTGTAC CAGGAATATC 
TTACCAGATG AAGGCGAGCA AGCAGATGAA GAAACCACTG GAGACATCAG TGATTCAATG 
GATTTTGTGC TGCTGAACTT TGCTGAGATG AACAAACTTT GGGTGCGAAT GCAACACCAG 
GGCCATAGTC GGGACAGAGA GAAAAGGGAG CGAGAAAGGC AGGAACTGAG AATCCTAGTA 
GGAACAAACC TGGTTCGCCT CAGTCAGTTG GAAGGTGTTA ATGTGGAGCG ATATAAGCAG 
ATTGTTCTGC CCGGAATATT GGAGCAAGTT GTAAACTGTA GAGATGCTTT AGCTCAGGAG 
TACCTCATGG AGTGCATCAT ACAGGTTTTC CCAGATGAAT TTCACCTCCA AACCTTGAAC 
CCATTTCTCA GAGCCTGTGC TGAGCTGCAC CAAAATGTGA ATGTGAAAAA TATAATTATT 
GCTTTAATTG ACAGGTTAGC TTTATTTGCA CATCGTGAAG ATGGACCTGG TATCCCAGCA 
GATATCAAAC TGTTTGACAT CTTTTCACAG CAGGTTGCTA CTGTAATACA GTCTCGCCAG 
GACATGCCCT CAGAGGATGT TGTATCTTTG CAAGTTTCTC TCATTAACTT GGCTATGAAA 
TGCTATCCAG ACCGTGTTGA CTATGTTGAT AAGGTGTTGG AGACAACTGT GGAAATATTC 
AATAAACTTA ATCTGGAACA TATTGCAACA AGCAGTGCTG TTTCAAAGGA GCTGACTAGA 
CTTTTGAAAA TCCCTATTGA TACTTACAAC AATATCCTGA CAGTTCTGAA ACTAAAACAT 
TTTCACCCAC TCTTTGAATA CTTTGATTAC GAGTCCAGGA AGAGCATGAG TTGTTACGTG 
CTTAGCAATG TATTGGATTA TAACACAGAA ATTGTGTCCC AAGAACAGGT TGATGCTATC 
ATGAATTTGG TATCCACACT GATCCAGGAT CAACCGGATC AGCCTGCTGA AGATCCTGAC 
CCTGAGGATT TTGCTGATGA GCAGAGTCTT GTGGGAAGAT TTATTCATCT TTTGCGTTCA 
GATGACCCTG ACCAACAGTA TCTGATCCTA AATACTGCCC GGAAACACTT TGGTGCAGGT 
GGGAACCAAC GTATTCGTTT TACACTGCCA CCACTGGTTT TTGCTGCATA CCAGCTTGCT 
TTTCGCTACA AGGAGAACTC CAAAGTGGAT GACAAATGGG AAAAGAAGTG CCAGAAGATC 
TTCTCGTTTG CTCATCAGAC CATCAGTGCT CTGATCAAAG CAGAACTGGC AGAGCTGCCT 
CTTCGTCTCT TCCTACAAGG AGCACTGGCT GCAGGAGAGA TTGGCTTTGA AAATCATGAA 
ACTGTGGCCT ATGAATTCAT GTCCCAGGCT TTCTCTCTAT ATGAAGATGA AATCAGTGAT 
TCAAAAGCAC AGCTGGCTGC AATCACCTTG ATAATTGGGA CATTTGAGAG GATGAAGTGC 
TTCAGTGAGG AGAACCATGA GCCTTTGAGG ACTCAGTGTG CGCTGGCAGC CTCTAAGCTC 
CTTAAGAAGC CAGACCAATG CCGGGCTGTG AGCACTTGTG CCCATCTGTT TTGGTCTGGC 
CGAAATACTG ACAAGAATGG AGAGGAGCTT CATGGAGGAA AAAGAGTGAT GGAATGCCTA 
AAGAAGGCTC TGAAGATAGC AAATCAGTGC ATGGATCCTT CTCTGCAAGT CCAACTTTTC 
ATAGAAATTC TGAATAGATA TATCTATTTT TATGAAAAGG AAAATGAGGC GGTGACAATT 
CAGGTTTTGA ATCAGCTTAT ACAGAAGATA AGAGAAGATC TCCCAAACCT TGAGTCTACT 
GAAGAAACAG AACAGATTAA CAAACACTTT CACAACACAC TGGAGCACTT GCGTCTGAGG 
AGGGAATCAC CAGAATCTGA GGGGCCAATT TATGAAGGTC TTGTTCTTTA G