ALAS1 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	ALAS1
Entrez Gene ID	105400157
Full Name	5'-aminolevulinate synthase 1
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011573631.1	XP_011571933.1	5-aminolevulinate synthase, nonspecific, mitochondrial

Pathways from BioSystems
Gene Ontology

The following ALAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq13701	XM_011573631.1 Latest version!	Aquila chrysaetos canadensis 5'-aminolevulinate synthase 1 (ALAS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALAS1 ( XM_011573631.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011571933.1 Aquila chrysaetos canadensis 5'-aminolevulinate synthase 1 (ALAS1), mRNA.

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CloneID	OAq13701
Clone ID Related Accession (Same CDS sequence)	XM_011573631.1
Accession Version	XM_011573631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	5-aminolevulinate synthase, nonspecific, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG TGGTGCGACG CTGCCCGTTC CTGGCCCGCG TCTCGCAGGC CTTCCTGCAG 
AAGGCCGGGC CCTCCCTGCT CCTCTACGCC CAGCACTGCC CCCGGATGAT GGAGGCGGCT 
CCCCCGGCCG CCGCCCGCGC CCTGGCCACG TCCGCCGCCC GCGGGCAGCA GGCGGAGGAG 
GAGAAGACCC CCGCGGCCCG CCGGGAGGCC AAAAATGCCA AAGAGGTGGC CCAACAGAAT 
ACAGATGGAA CCCAGCCACC TACTGGCCAC CCACCTGCTG GTGTTAGCCA GAGCTCTGCT 
ACCAAATGCC CATTCCTGGC AGCTCAGATG AATCACAAGA ACAGTAATGT TTTCTGCAAA 
GCCAGCCTAG AACTCCAAGA GGATGTGCAG GAAATGCAGG CGGAGAGGAA AGGTACAGAA 
TTTGCCAAAA TACCAACTAC TTCCACACTG AGGAACATTG AGACTGAGGG AGAAGAGCGG 
AGTGGCTTGC TCAAGAAGTT TAAGGATATT GTGCTGAAGC AAAGACCAGA AAGTGTGTCT 
CATCTGCTTC AGGATAACTT GCCAAAATCT GTATCCACCT TCCAGTATGA TCAGTTCTTT 
GAGAAAAAGA TAGATGAAAA GAAGAAGGAT CACACCTACC GAGTATTCAA AACAGTCAAC 
CGAAAGGCAC AAATCTTTCC CATGGCAGAT GACTATACTG ATTCTCTGAT CACCAAGAAA 
GAGGTGTCTG TCTGGTGCAG CAATGATTAC CTGGGGATGA GTCGTCACCC TCGCGTGTGT 
GGAGCAGTTA TGGATACACT GAAACAACAT GGTGCTGGAG CAGGAGGCAC AAGAAATATT 
TCAGGAACAA GTAAATTTCA TGTCGATTTG GAAAAAGAAC TAGCTGATCT TCATGGAAAA 
GATGCAGCAC TGTTGTTCTC ATCTTGCTTT GTAGCCAATG ACTCCACTCT CTTCACTCTA 
GCTAAAATGC TGCCAGGCTG TGAGATCTAC TCTGATTCTG GAAACCATGC CTCCATGATC 
CAGGGGATCC GAAACAGCAG GGTGCCAAAA CACATATTTC GCCATAATGA TGTCAACCAT 
CTTCGAGAAC TATTGAAGAA GTCTGATCCT TCTACCCCTA AAATTGTTGC ATTTGAAACT 
GTTCACTCAA TGGATGGTGC AGTCTGTCCT CTGGAAGAGC TGTGTGATGT GGCCCACGAG 
CACGGGGCAA TCACTTTTGT GGATGAAGTG CATGCTGTGG GGCTATATGG AGCTCGAGGT 
GGTGGTATAG GAGACCGGGA TGGAATCATG CACAAGATGG ACATCATCTC TGGAACGCTT 
GGCAAAGCAT TTGGTTGCGT AGGAGGATAC ATCTCCAGTA CAAGTTCTCT GATAGACACT 
GTTCGTTCAT ATGCTGCTGG CTTTATTTTC ACAACGTCCC TGCCACCCAT GCTCTTAGCC 
GGTGCCCTAG AATCTGTCCG AACTCTGAAG AGCACAGAGG GGCAAGTTCT GAGGCGCCAG 
CATCAACGCA ATGTGAAGCT TATGAGACAG ATGCTGATGG ATGCGGGCCT TCCTGTGGTG 
CACTGCCCCA GTCACATCAT TCCAATAAGG GTTGCAGATG CTGCTAAAAA TACAGAGATC 
TGTGACAAGC TGATGAGCCA GCACAGCATC TATGTCCAAG CAATCAACTA CCCCACAGTT 
CCCCGTGGAG AAGAGCTGCT ACGTATTGCC CCTACGCCTC ACCACACCCC TCAGATGATG 
AGTTATTTTC TTGAAAAACT GCTAGCTACA TGGAAGGATG TTGGCCTGGA GCTGAAACCG 
CACTCGTCAG CTGAATGCAA CTTCTGTAGA AGACCCCTAC ATTTTGAACT GATGAGTGAA 
CGGGAAAGAT CCTACTTCAG TGGCATGAGC AAACTAGTAT CTGTCAGTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011571933.1
CDS	127..2040
Translation	MEAVVRRCPFLARVSQAFLQKAGPSLLLYAQHCPRMMEAAPPAAARALATSAARGQQAEEEKTPAARREAKNAKEVAQQNTDGTQPPTGHPPAGVSQSSATKCPFLAAQMNHKNSNVFCKASLELQEDVQEMQAERKGTEFAKIPTTSTLRNIETEGEERSGLLKKFKDIVLKQRPESVSHLLQDNLPKSVSTFQYDQFFEKKIDEKKKDHTYRVFKTVNRKAQIFPMADDYTDSLITKKEVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEKELADLHGKDAALLFSSCFVANDSTLFTLAKMLPGCEIYSDSGNHASMIQGIRNSRVPKHIFRHNDVNHLRELLKKSDPSTPKIVAFETVHSMDGAVCPLEELCDVAHEHGAITFVDEVHAVGLYGARGGGIGDRDGIMHKMDIISGTLGKAFGCVGGYISSTSSLIDTVRSYAAGFIFTTSLPPMLLAGALESVRTLKSTEGQVLRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPIRVADAAKNTEICDKLMSQHSIYVQAINYPTVPRGEELLRIAPTPHHTPQMMSYFLEKLLATWKDVGLELKPHSSAECNFCRRPLHFELMSERERSYFSGMSKLVSVSA

Target ORF information:

RefSeq Version	XM_011573631.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis 5'-aminolevulinate synthase 1 (ALAS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011573631.1

ORF Insert Sequence:

ATGGAGGCGG TGGTGCGACG CTGCCCGTTC CTGGCCCGCG TCTCGCAGGC CTTCCTGCAG 
AAGGCCGGGC CCTCCCTGCT CCTCTACGCC CAGCACTGCC CCCGGATGAT GGAGGCGGCT 
CCCCCGGCCG CCGCCCGCGC CCTGGCCACG TCCGCCGCCC GCGGGCAGCA GGCGGAGGAG 
GAGAAGACCC CCGCGGCCCG CCGGGAGGCC AAAAATGCCA AAGAGGTGGC CCAACAGAAT 
ACAGATGGAA CCCAGCCACC TACTGGCCAC CCACCTGCTG GTGTTAGCCA GAGCTCTGCT 
ACCAAATGCC CATTCCTGGC AGCTCAGATG AATCACAAGA ACAGTAATGT TTTCTGCAAA 
GCCAGCCTAG AACTCCAAGA GGATGTGCAG GAAATGCAGG CGGAGAGGAA AGGTACAGAA 
TTTGCCAAAA TACCAACTAC TTCCACACTG AGGAACATTG AGACTGAGGG AGAAGAGCGG 
AGTGGCTTGC TCAAGAAGTT TAAGGATATT GTGCTGAAGC AAAGACCAGA AAGTGTGTCT 
CATCTGCTTC AGGATAACTT GCCAAAATCT GTATCCACCT TCCAGTATGA TCAGTTCTTT 
GAGAAAAAGA TAGATGAAAA GAAGAAGGAT CACACCTACC GAGTATTCAA AACAGTCAAC 
CGAAAGGCAC AAATCTTTCC CATGGCAGAT GACTATACTG ATTCTCTGAT CACCAAGAAA 
GAGGTGTCTG TCTGGTGCAG CAATGATTAC CTGGGGATGA GTCGTCACCC TCGCGTGTGT 
GGAGCAGTTA TGGATACACT GAAACAACAT GGTGCTGGAG CAGGAGGCAC AAGAAATATT 
TCAGGAACAA GTAAATTTCA TGTCGATTTG GAAAAAGAAC TAGCTGATCT TCATGGAAAA 
GATGCAGCAC TGTTGTTCTC ATCTTGCTTT GTAGCCAATG ACTCCACTCT CTTCACTCTA 
GCTAAAATGC TGCCAGGCTG TGAGATCTAC TCTGATTCTG GAAACCATGC CTCCATGATC 
CAGGGGATCC GAAACAGCAG GGTGCCAAAA CACATATTTC GCCATAATGA TGTCAACCAT 
CTTCGAGAAC TATTGAAGAA GTCTGATCCT TCTACCCCTA AAATTGTTGC ATTTGAAACT 
GTTCACTCAA TGGATGGTGC AGTCTGTCCT CTGGAAGAGC TGTGTGATGT GGCCCACGAG 
CACGGGGCAA TCACTTTTGT GGATGAAGTG CATGCTGTGG GGCTATATGG AGCTCGAGGT 
GGTGGTATAG GAGACCGGGA TGGAATCATG CACAAGATGG ACATCATCTC TGGAACGCTT 
GGCAAAGCAT TTGGTTGCGT AGGAGGATAC ATCTCCAGTA CAAGTTCTCT GATAGACACT 
GTTCGTTCAT ATGCTGCTGG CTTTATTTTC ACAACGTCCC TGCCACCCAT GCTCTTAGCC 
GGTGCCCTAG AATCTGTCCG AACTCTGAAG AGCACAGAGG GGCAAGTTCT GAGGCGCCAG 
CATCAACGCA ATGTGAAGCT TATGAGACAG ATGCTGATGG ATGCGGGCCT TCCTGTGGTG 
CACTGCCCCA GTCACATCAT TCCAATAAGG GTTGCAGATG CTGCTAAAAA TACAGAGATC 
TGTGACAAGC TGATGAGCCA GCACAGCATC TATGTCCAAG CAATCAACTA CCCCACAGTT 
CCCCGTGGAG AAGAGCTGCT ACGTATTGCC CCTACGCCTC ACCACACCCC TCAGATGATG 
AGTTATTTTC TTGAAAAACT GCTAGCTACA TGGAAGGATG TTGGCCTGGA GCTGAAACCG 
CACTCGTCAG CTGAATGCAA CTTCTGTAGA AGACCCCTAC ATTTTGAACT GATGAGTGAA 
CGGGAAAGAT CCTACTTCAG TGGCATGAGC AAACTAGTAT CTGTCAGTGC ATGA