BRAF cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAF
Entrez Gene ID	106488842
Full Name	B-Raf proto-oncogene, serine/threonine kinase
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013947908.1	XP_013803362.1	serine/threonine-protein kinase B-raf isoform X1
XM_013947909.1	XP_013803363.1	serine/threonine-protein kinase B-raf isoform X2

Pathways from BioSystems
Gene Ontology

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp18071	XM_013947908.1 Latest version!	Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAp18072	XM_013947909.1 Latest version!	Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAF ( XM_013947908.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013803362.1 Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp18071
Clone ID Related Accession (Same CDS sequence)	XM_013947908.1
Accession Version	XM_013947908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013995860.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAAT TAACACAAGA ACATATAGAG GCACTGCTAG ACAAGTTTGG AGGAGAGCAT 
AACCCACCAT CAATATACTT AGAGGCCTAT GAAGAGTACA CCAGTAAACT AGATGCCCTA 
CAACAGAGGG AACAGCAGTT ATTGGAGTCC ATGGGGAACG GAACTGATTT CTCTGTCTCC 
AGTTCGGCTT CAACAGACAC AGTTGCGTCA TCTTCCTCCT CTAGCCTCTC CGTAGCACCT 
TCATCCCTTT CAGTTTATCA AAACCCTACT GATATGTCAC GGAATAACCC TAAGTCTCCG 
CAGAAGCCTA TCGTTAGAGT CTTCCTACCC AACAAGCAAA GGACTGTGGT TCCGGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGCCTGAAG AAAGCTCTGA TGATGAGAGG TCTTATTCCA 
GAATGTTGTG CTGTTTACAG AATACAGGAT GGAGAGAAGA AGCCGATTGG CTGGGACACT 
GACATATCCT GGCTCACAGG AGAGGAGTTG CACGTGGAAG TCCTGGAGAA CGTGCCACTC 
ACAACACACA ATTTTGTTCG AAAGACGTTC TTCACGTTAG CGTTCTGCGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGATT CCGGTGCCAG ACATGTGGCT ATAAATTTCA CCAGCGCTGT 
AGTACAGAAG TGCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGTT GTTTGTCTCC 
AAGTTCTTTG AGCATCACCC CATACCGCAG GAGGAGACCT CCTTGGGAGA GACCACCCCA 
GCATCTGGAT CGTACTCCTC ATTGCCCCCC TCAGATTCTG TTGGACCACC AATTCTCCCT 
AGTCCTTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAC 
CATCGGAATC AATTTGGGCA ACGTGACCGA TCGTCTTCAG CCCCCAATGT TCACATCAAT 
ACAATCGAGC CAGTCAATAT TGATGACTTG ATTAGAGACC AGAGTTTACG AGGAGAGGGA 
GCCCCTTTGA ACCAGCTGAT GCGCTGTCTT CGGAAATACC AATCCCGGAC TCCCAGTCCC 
CTCCTTCATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGTTCAACTG CAGGTTTGTC TGCAACCCCT CCTGCCTCTT TGCCTGGGTC ACTCACCAAT 
GTGAAAGCGT TACAGAAATC ACCAGGACCC CAACGGGAGA GGAAGTCATC CTCATCCTCA 
GAAGACAGAA ATAGAATGAA AACCCTCGGT CGACGGGATT CAAGTGATGA TTGGGAGATA 
CCAGATGGAC AGATCACAGT TGGACAAAGG ATAGGGTCTG GATCGTTTGG AACGGTCTAC 
AAAGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTTACGGC ACCTACACCT 
CAACAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACACG ACATGTGAAT 
ATCCTGCTTT TCATGGGTTA CTCAACAAAG CCCCAGTTGG CTATTGTTAC ACAATGGTGT 
GAGGGGTCAA GCTTATATCA CCATCTGCAC ATCATTGAGA CCAAATTTGA AATGATCAAA 
CTAATTGACA TTGCACGGCA GACTGCACAA GGCATGGATT ATTTGCATGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTTAAAATA 
GGTGACTTCG GTCTAGCTAC AGTAAAATCA CGATGGAGTG GATCCCATCA GTTTGAACAG 
CTGTCTGGAT CTATTCTATG GATGGCACCA GAAGTTATTA GGATGCAAGA TAAAAACCCC 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTC TGTACGAATT GATGACTGGA 
CAGTTACCAT ACTCAAACAT CAACAACAGG GACCAGATAA TATTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG CAAAGTACGG AGCAACTGTC CAAAAGCTAT GAAGAGACTA 
ATGGCAGAAT GTTTAAAAAA GAAAAGAGAT GAGAGACCCC TTTTTCCACA GATTCTGGCC 
TCCATTGAGC TTCTGGCCCG GTCATTGCCA AAAATTCACC GCAGTGCATC TGAGCCCTCA 
TTAAACCGGG CTGGCTTCCA GACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AAGCAGGGGG ATACGGAGAA TTTGCAGCGT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013803362.1
CDS	15..2282
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVASSSSSSLSVAPSSLSVYQNPTDMSRNNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLGETTPASGSYSSLPPSDSVGPPILPSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQSLRGEGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTAGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_013947908.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013947908.1

ORF Insert Sequence:

ATGATTAAAT TAACACAAGA ACATATAGAG GCACTGCTAG ACAAGTTTGG AGGAGAGCAT 
AACCCACCAT CAATATACTT AGAGGCCTAT GAAGAGTACA CCAGTAAACT AGATGCCCTA 
CAACAGAGGG AACAGCAGTT ATTGGAGTCC ATGGGGAACG GAACTGATTT CTCTGTCTCC 
AGTTCGGCTT CAACAGACAC AGTTGCGTCA TCTTCCTCCT CTAGCCTCTC CGTAGCACCT 
TCATCCCTTT CAGTTTATCA AAACCCTACT GATATGTCAC GGAATAACCC TAAGTCTCCG 
CAGAAGCCTA TCGTTAGAGT CTTCCTACCC AACAAGCAAA GGACTGTGGT TCCGGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGCCTGAAG AAAGCTCTGA TGATGAGAGG TCTTATTCCA 
GAATGTTGTG CTGTTTACAG AATACAGGAT GGAGAGAAGA AGCCGATTGG CTGGGACACT 
GACATATCCT GGCTCACAGG AGAGGAGTTG CACGTGGAAG TCCTGGAGAA CGTGCCACTC 
ACAACACACA ATTTTGTTCG AAAGACGTTC TTCACGTTAG CGTTCTGCGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGATT CCGGTGCCAG ACATGTGGCT ATAAATTTCA CCAGCGCTGT 
AGTACAGAAG TGCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGTT GTTTGTCTCC 
AAGTTCTTTG AGCATCACCC CATACCGCAG GAGGAGACCT CCTTGGGAGA GACCACCCCA 
GCATCTGGAT CGTACTCCTC ATTGCCCCCC TCAGATTCTG TTGGACCACC AATTCTCCCT 
AGTCCTTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAC 
CATCGGAATC AATTTGGGCA ACGTGACCGA TCGTCTTCAG CCCCCAATGT TCACATCAAT 
ACAATCGAGC CAGTCAATAT TGATGACTTG ATTAGAGACC AGAGTTTACG AGGAGAGGGA 
GCCCCTTTGA ACCAGCTGAT GCGCTGTCTT CGGAAATACC AATCCCGGAC TCCCAGTCCC 
CTCCTTCATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGTTCAACTG CAGGTTTGTC TGCAACCCCT CCTGCCTCTT TGCCTGGGTC ACTCACCAAT 
GTGAAAGCGT TACAGAAATC ACCAGGACCC CAACGGGAGA GGAAGTCATC CTCATCCTCA 
GAAGACAGAA ATAGAATGAA AACCCTCGGT CGACGGGATT CAAGTGATGA TTGGGAGATA 
CCAGATGGAC AGATCACAGT TGGACAAAGG ATAGGGTCTG GATCGTTTGG AACGGTCTAC 
AAAGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTTACGGC ACCTACACCT 
CAACAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACACG ACATGTGAAT 
ATCCTGCTTT TCATGGGTTA CTCAACAAAG CCCCAGTTGG CTATTGTTAC ACAATGGTGT 
GAGGGGTCAA GCTTATATCA CCATCTGCAC ATCATTGAGA CCAAATTTGA AATGATCAAA 
CTAATTGACA TTGCACGGCA GACTGCACAA GGCATGGATT ATTTGCATGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTTAAAATA 
GGTGACTTCG GTCTAGCTAC AGTAAAATCA CGATGGAGTG GATCCCATCA GTTTGAACAG 
CTGTCTGGAT CTATTCTATG GATGGCACCA GAAGTTATTA GGATGCAAGA TAAAAACCCC 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTC TGTACGAATT GATGACTGGA 
CAGTTACCAT ACTCAAACAT CAACAACAGG GACCAGATAA TATTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG CAAAGTACGG AGCAACTGTC CAAAAGCTAT GAAGAGACTA 
ATGGCAGAAT GTTTAAAAAA GAAAAGAGAT GAGAGACCCC TTTTTCCACA GATTCTGGCC 
TCCATTGAGC TTCTGGCCCG GTCATTGCCA AAAATTCACC GCAGTGCATC TGAGCCCTCA 
TTAAACCGGG CTGGCTTCCA GACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AAGCAGGGGG ATACGGAGAA TTTGCAGCGT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_013947909.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013803363.1 Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAp18072
Clone ID Related Accession (Same CDS sequence)	XM_013947909.1
Accession Version	XM_013947909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013995860.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAAT TAACACAAGA ACATATAGAG GCACTGCTAG ACAAGTTTGG AGGAGAGCAT 
AACCCACCAT CAATATACTT AGAGGCCTAT GAAGAGTACA CCAGTAAACT AGATGCCCTA 
CAACAGAGGG AACAGCAGTT ATTGGAGTCC ATGGGGAACG GAACTGATTT CTCTGTCTCC 
AGTTCGGCTT CAACAGACAC AGTTGCGTCA TCTTCCTCCT CTAGCCTCTC CGTAGCACCT 
TCATCCCTTT CAGTTTATCA AAACCCTACT GATATGTCAC GGAATAACCC TAAGTCTCCG 
CAGAAGCCTA TCGTTAGAGT CTTCCTACCC AACAAGCAAA GGACTGTGGT TCCGGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGCCTGAAG AAAGCTCTGA TGATGAGAGG TCTTATTCCA 
GAATGTTGTG CTGTTTACAG AATACAGGAT GGAGAGAAGA AGCCGATTGG CTGGGACACT 
GACATATCCT GGCTCACAGG AGAGGAGTTG CACGTGGAAG TCCTGGAGAA CGTGCCACTC 
ACAACACACA ATTTTGTTCG AAAGACGTTC TTCACGTTAG CGTTCTGCGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGATT CCGGTGCCAG ACATGTGGCT ATAAATTTCA CCAGCGCTGT 
AGTACAGAAG TGCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGTT GTTTGTCTCC 
AAGTTCTTTG AGCATCACCC CATACCGCAG GAGGAGACCT CCTTGGGAGA GACCACCCCA 
GCATCTGGAT CGTACTCCTC ATTGCCCCCC TCAGATTCTG TTGGACCACC AATTCTCCCT 
AGTCCTTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAC 
CATCGGAATC AATTTGGGCA ACGTGACCGA TCGTCTTCAG CCCCCAATGT TCACATCAAT 
ACAATCGAGC CAGTCAATAT TGATGACTTG ATTAGAGACC AGAGTTTACG AGGAGAGGGA 
GGTTCAACTG CAGGTTTGTC TGCAACCCCT CCTGCCTCTT TGCCTGGGTC ACTCACCAAT 
GTGAAAGCGT TACAGAAATC ACCAGGACCC CAACGGGAGA GGAAGTCATC CTCATCCTCA 
GAAGACAGAA ATAGAATGAA AACCCTCGGT CGACGGGATT CAAGTGATGA TTGGGAGATA 
CCAGATGGAC AGATCACAGT TGGACAAAGG ATAGGGTCTG GATCGTTTGG AACGGTCTAC 
AAAGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTTACGGC ACCTACACCT 
CAACAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACACG ACATGTGAAT 
ATCCTGCTTT TCATGGGTTA CTCAACAAAG CCCCAGTTGG CTATTGTTAC ACAATGGTGT 
GAGGGGTCAA GCTTATATCA CCATCTGCAC ATCATTGAGA CCAAATTTGA AATGATCAAA 
CTAATTGACA TTGCACGGCA GACTGCACAA GGCATGGATT ATTTGCATGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTTAAAATA 
GGTGACTTCG GTCTAGCTAC AGTAAAATCA CGATGGAGTG GATCCCATCA GTTTGAACAG 
CTGTCTGGAT CTATTCTATG GATGGCACCA GAAGTTATTA GGATGCAAGA TAAAAACCCC 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTC TGTACGAATT GATGACTGGA 
CAGTTACCAT ACTCAAACAT CAACAACAGG GACCAGATAA TATTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG CAAAGTACGG AGCAACTGTC CAAAAGCTAT GAAGAGACTA 
ATGGCAGAAT GTTTAAAAAA GAAAAGAGAT GAGAGACCCC TTTTTCCACA GATTCTGGCC 
TCCATTGAGC TTCTGGCCCG GTCATTGCCA AAAATTCACC GCAGTGCATC TGAGCCCTCA 
TTAAACCGGG CTGGCTTCCA GACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AAGCAGGGGG ATACGGAGAA TTTGCAGCGT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013803363.1
CDS	1..2148
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESMGNGTDFSVSSSASTDTVASSSSSSLSVAPSSLSVYQNPTDMSRNNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLGETTPASGSYSSLPPSDSVGPPILPSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQSLRGEGGSTAGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_013947909.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013947909.1

ORF Insert Sequence:

ATGATTAAAT TAACACAAGA ACATATAGAG GCACTGCTAG ACAAGTTTGG AGGAGAGCAT 
AACCCACCAT CAATATACTT AGAGGCCTAT GAAGAGTACA CCAGTAAACT AGATGCCCTA 
CAACAGAGGG AACAGCAGTT ATTGGAGTCC ATGGGGAACG GAACTGATTT CTCTGTCTCC 
AGTTCGGCTT CAACAGACAC AGTTGCGTCA TCTTCCTCCT CTAGCCTCTC CGTAGCACCT 
TCATCCCTTT CAGTTTATCA AAACCCTACT GATATGTCAC GGAATAACCC TAAGTCTCCG 
CAGAAGCCTA TCGTTAGAGT CTTCCTACCC AACAAGCAAA GGACTGTGGT TCCGGCAAGA 
TGTGGGGTGA CAGTCCGAGA CAGCCTGAAG AAAGCTCTGA TGATGAGAGG TCTTATTCCA 
GAATGTTGTG CTGTTTACAG AATACAGGAT GGAGAGAAGA AGCCGATTGG CTGGGACACT 
GACATATCCT GGCTCACAGG AGAGGAGTTG CACGTGGAAG TCCTGGAGAA CGTGCCACTC 
ACAACACACA ATTTTGTTCG AAAGACGTTC TTCACGTTAG CGTTCTGCGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGATT CCGGTGCCAG ACATGTGGCT ATAAATTTCA CCAGCGCTGT 
AGTACAGAAG TGCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGTT GTTTGTCTCC 
AAGTTCTTTG AGCATCACCC CATACCGCAG GAGGAGACCT CCTTGGGAGA GACCACCCCA 
GCATCTGGAT CGTACTCCTC ATTGCCCCCC TCAGATTCTG TTGGACCACC AATTCTCCCT 
AGTCCTTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAC 
CATCGGAATC AATTTGGGCA ACGTGACCGA TCGTCTTCAG CCCCCAATGT TCACATCAAT 
ACAATCGAGC CAGTCAATAT TGATGACTTG ATTAGAGACC AGAGTTTACG AGGAGAGGGA 
GGTTCAACTG CAGGTTTGTC TGCAACCCCT CCTGCCTCTT TGCCTGGGTC ACTCACCAAT 
GTGAAAGCGT TACAGAAATC ACCAGGACCC CAACGGGAGA GGAAGTCATC CTCATCCTCA 
GAAGACAGAA ATAGAATGAA AACCCTCGGT CGACGGGATT CAAGTGATGA TTGGGAGATA 
CCAGATGGAC AGATCACAGT TGGACAAAGG ATAGGGTCTG GATCGTTTGG AACGGTCTAC 
AAAGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTTACGGC ACCTACACCT 
CAACAGTTAC AGGCTTTCAA AAATGAAGTA GGAGTGCTCA GGAAAACACG ACATGTGAAT 
ATCCTGCTTT TCATGGGTTA CTCAACAAAG CCCCAGTTGG CTATTGTTAC ACAATGGTGT 
GAGGGGTCAA GCTTATATCA CCATCTGCAC ATCATTGAGA CCAAATTTGA AATGATCAAA 
CTAATTGACA TTGCACGGCA GACTGCACAA GGCATGGATT ATTTGCATGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTTAAAATA 
GGTGACTTCG GTCTAGCTAC AGTAAAATCA CGATGGAGTG GATCCCATCA GTTTGAACAG 
CTGTCTGGAT CTATTCTATG GATGGCACCA GAAGTTATTA GGATGCAAGA TAAAAACCCC 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTC TGTACGAATT GATGACTGGA 
CAGTTACCAT ACTCAAACAT CAACAACAGG GACCAGATAA TATTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG CAAAGTACGG AGCAACTGTC CAAAAGCTAT GAAGAGACTA 
ATGGCAGAAT GTTTAAAAAA GAAAAGAGAT GAGAGACCCC TTTTTCCACA GATTCTGGCC 
TCCATTGAGC TTCTGGCCCG GTCATTGCCA AAAATTCACC GCAGTGCATC TGAGCCCTCA 
TTAAACCGGG CTGGCTTCCA GACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AAGCAGGGGG ATACGGAGAA TTTGCAGCGT TCAAGTAG