Ef-1a-f1 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ef-1a-f1
Entrez Gene ID	408385
Full Name	translation elongation factor eEF-1 alpha chain
Synonyms	GB10560
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006566844.2	XP_006566907.1	elongation factor 1-alpha isoform X1
XM_006566843.2	XP_006566906.1	elongation factor 1-alpha isoform X1
XM_006566844.3	XP_006566907.1	elongation factor 1-alpha isoform X1
XM_006566843.3	XP_006566906.1	elongation factor 1-alpha isoform X1
XM_006566845.2	XP_006566908.1	elongation factor 1-alpha isoform X1
NM_001011628.2	NP_001011628.1	elongation factor 1-alpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Evaluating alternative hypotheses for the early evolution and diversification of ants.
Brady SG, Schultz TR, Fisher BL, Ward PS
Proceedings of the National Academy of Sciences of the United States of America103(48)18172-7(2006 Nov)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Apis mellifera cytoplasmic elongation factor 1 alpha (EF-1 alpha) is closely related to Drosophila melanogaster EF-1 alpha.
Walldorf U, Hovemann BT
FEBS letters267(2)245-9(1990 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ef-1a-f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ef-1a-f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd00183	NM_001011628.2 Latest version!	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHd13548	XM_006566844.3 Latest version!	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHd13548	XM_006566845.2 Latest version!	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHd13548	XM_006566843.3 Latest version!	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHd13548	XM_006566844.2	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHd13548	XM_006566843.2	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ef-1a-f1 ( NM_001011628.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011628.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), mRNA.

Price & Availability↑

CloneID	OHd00183
Clone ID Related Accession (Same CDS sequence)	NM_001011628.2
Accession Version	NM_001011628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X52884.1. On Mar 4, 2010 this sequence version replaced NM_001011628.1. ##Evidence-Data-START## CDS exon combination :: HP478552.1, HP524564.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00113341 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCC 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCCCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACTGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011628.1
CDS	1..1386
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	NM_001011628.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011628.2

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCC 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCCCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACTGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ef-1a-f1 ( XM_006566844.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006566907.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHd13548
Clone ID Related Accession (Same CDS sequence)	XM_006566844.2 , XM_006566843.2 , XM_006566844.3 , XM_006566843.3 , XM_006566845.2
Accession Version	XM_006566844.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006566844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006566907.1
CDS	305..1690
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	XM_006566844.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006566844.2

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ef-1a-f1 ( XM_006566843.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006566906.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd13548
Clone ID Related Accession (Same CDS sequence)	XM_006566844.2 , XM_006566843.2 , XM_006566844.3 , XM_006566843.3 , XM_006566845.2
Accession Version	XM_006566843.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006566843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006566906.1
CDS	148..1533
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	XM_006566843.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006566843.2

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ef-1a-f1 ( XM_006566844.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006566907.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHd13548
Clone ID Related Accession (Same CDS sequence)	XM_006566844.2 , XM_006566843.2 , XM_006566844.3 , XM_006566843.3 , XM_006566845.2
Accession Version	XM_006566844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037649.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006566844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006566907.1
CDS	292..1677
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	XM_006566844.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006566844.3

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ef-1a-f1 ( XM_006566843.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006566906.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd13548
Clone ID Related Accession (Same CDS sequence)	XM_006566844.2 , XM_006566843.2 , XM_006566844.3 , XM_006566843.3 , XM_006566845.2
Accession Version	XM_006566843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037649.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006566843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006566906.1
CDS	172..1557
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	XM_006566843.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006566843.3

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ef-1a-f1 ( XM_006566845.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006566908.1 Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHd13548
Clone ID Related Accession (Same CDS sequence)	XM_006566844.2 , XM_006566843.2 , XM_006566844.3 , XM_006566843.3 , XM_006566845.2
Accession Version	XM_006566845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	elongation factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006566845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006566908.1
CDS	163..1548
Translation	MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQKKK

Target ORF information:

RefSeq Version	XM_006566845.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera translation elongation factor eEF-1 alpha chain (Ef-1a-f1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006566845.2

ORF Insert Sequence:

ATGGGTAAGG AGAAGATTCA TATAAACATC GTGGTGATCG GCCACGTTGA TTCGGGCAAG 
TCGACGACTA CCGGTCATCT GATTTACAAA TGCGGCGGCA TCGACAAGCG TACCATCGAA 
AAGTTCGAGA AGGAGGCTCA AGAAATGGGC AAGGGCTCGT TCAAGTACGC CTGGGTGTTG 
GACAAGCTGA AGGCTGAACG CGAGCGTGGT ATCACGATCG ATATCGCCCT GTGGAAGTTC 
GAGACTGCGA AATATTACGT GACGATCATA GACGCGCCTG GGCATCGCGA TTTCATCAAA 
AATATGATCA CCGGGACGAG TCAGGCCGAT TGTGCCGTGT TAATAGTCGC GGCTGGTATA 
GGTGAATTCG AAGCTGGTAT CTCGAAGAAC GGACAGACGC GCGAACACGC TTTGCTGGCG 
TTCACGCTTG GGGTCAAGCA GTTGATCGTT GGAGTGAACA AGATGGACAT GACCGATCCT 
CCGTATTCGG AGGCCCGTTT CGAGGAGATC AAGAAAGAGG TGTCATCGTA CATAAAAAAG 
ATCGGTTACA ACACAGCCTC GGTCGCGTTC GTACCGATCT CCGGATGGCA CGGCGACAAC 
ATGTTGGAAC CCTCGCCGAA GACACCGTGG TACAAGGGAT GGAAAGTGGA GCGCAAAGAT 
GGTAACGCTG ACGGAAAGAC CCTGATCGAA GCTCTCGACG CTATATTACC GCCGTCCAGA 
CCTACCGATA AGGCTCTTCG TTTACCGCTT CAGGACGTTT ATAAGATCGG TGGTATCGGT 
ACTGTACCGG TCGGTCGGGT GGAGACCGGT ATTTTGAAAC CAGGTATGCT GGTAACATTC 
GCTCCAGCAG CGCTCACCAC CGAAGTGAAA TCGGTCGAGA TGCATCACGA GGCATTGACG 
GAGGCGTTAC CCGGGGACAA CGTTGGCTTC AACGTGAAGA ACATTTCTGT GAAAGAGTTG 
AGGCGTGGTT ACGTGGCGGG AGATTCGAAA AATCAACCGC CACGGGGGGC AGCCGATTTC 
ACAGCGCAGG TGATCGTCCT CAATCATCCT GGGCAGATAA GCAACGGTTA TACACCGGTG 
CTCGATTGTC ACACCGCTCA TATCGCTTGC AAATTTGCGG AGATCAAGGA GAAATGCGAC 
CGCCGTACCG GCAAGACCAC CGAGGAGAAT CCGAAGAGCA TCAAGAGCGG AGACGCCGCG 
ATCGTGATGT TGCAACCTAC TAAGCCGATG TGCGTCGAAG CTTTCCAGGA ATTCCCTCCG 
CTTGGTCGCT TCGCGGTTCG CGACATGCGT CAGACCGTCG CTGTGGGCGT CATTAAGAGT 
GTCACCTTTA AAGATACCCA GGGCAAGGTC ACAAAGGCCG CGGAGAAAGC CCAGAAGAAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
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Ef-1a-f1 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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