LOC102675864 cDNA ORF clone, Apis dorsata(giant honeybee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102675864
Entrez Gene ID	102675864
Full Name	E3 ubiquitin-protein ligase TRIM33-like
Gene Type	protein-coding
Organism	Apis dorsata(giant honeybee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006613758.1	XP_006613821.1	E3 ubiquitin-protein ligase TRIM33-like
XM_006613758.2	XP_006613821.1	E3 ubiquitin-protein ligase TRIM33-like

Pathways from BioSystems
Gene Ontology

The following LOC102675864 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102675864 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAp66364	XM_006613758.2 Latest version!	Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OAp66364	XM_006613758.1	Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102675864 ( XM_006613758.1 ) cDNA ORF clone, Apis dorsata(giant honeybee) -> XP_006613821.1 Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.

Price & Availability↑

CloneID	OAp66364
Clone ID Related Accession (Same CDS sequence)	XM_006613758.1 , XM_006613758.2
Accession Version	XM_006613758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-01-07
Organism	Apis dorsata(giant honeybee)
Product	E3 ubiquitin-protein ligase TRIM33-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006263451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis dorsata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTC GAACAAATGA GTTATTAACA ACAGGTCCTG TAACGATTAA AGAAGAACCA 
CTTGATCAAA CTGGTGTGAA GCAAAACATG CAACAATCTC AAGTACAGGG CGTTTGCATT 
GAAGAAGAAT GTGAACAACG TATTGAATGT ACAACTGAGG GTCAAGAGAC TGAAACACGT 
GCATTTTTAT CGAAATGTGT ATTCTGTGGA AAGACATTTA CTTTTGGTGA TGATCCAAAA 
CTTTTGGAAT GTTTACATGC AGCATGTTCG ACATGTGTCA ATAGCAAACT TAGTGATCAC 
AATACATCGG TCGACGTGGA TGTTTTGTTG GAGAGCAATG TGATTACTTG CCAAATATGT 
AATGTAACAA CACAAGCAGA AAATTTAATT GAAAATCGAT TTTTATCAAA ATTTATTGAA 
GAAGATAATA GTCCAGGTAC TGATGATGAA TCTAAAGAAA CAGAAGAAGA GAAAAAATGT 
ACTAGCTGTC ACGATAATGC TAATGCCACA AGTTGGTGTG TAGAATGTGA AGAATTTATA 
TGTCAAAATT GTGTTATGGC ACATCAAAGA TTAAAAATAA CAAAAGATCA CACAATTAAA 
CCAAAGGATG AAGTTGCCAA TGATAGAGAT AGTGTTAAAA AAAGCAACAA AAAAATACCT 
GGCTACTTAT TTTGTACAAT TCATTCACAT GAACAATTAT CTTTATTTTG TCAAACATGT 
GATAAACTTA CTTGTAGAGA TTGTCAACTA AGTGAACATA GAGATCATAA GTACAAATTT 
ATTCATGAAA TTGCTGCTGA AACCCGTGCT TCTGTATCAA CTTTACTTAA AGAAGTGAGT 
TATAAACGAG TTTTATTAAA AAGTGCAATG AAAGTTATAG AAGATAGACA AGTTCTTATA 
TTAGAGAAAA AGAAAAATTT AGTACAAGAT ATAACACAAA TGGTGGTGCA ATTAACGAAT 
ACAATTAATA CACGAGGAAA ACAATTAGTT ATGCGTTTAA ATGAAGTTTG TGATCAAAAG 
CAAAATACAT TAAATGAAAA AAAAGTTGCT TTAGATCAAT TATCAAAACT TACAGATCAT 
TGTATTCAAT TTGTAACTCA TGCTTTAAAA AAAGGTTCAG ATATGGAATT ATTATATAGT 
AAAAAATCTG TTACAAGTCA TTTACAAAGA ATAAAAAGTA GACGAGCTGA TATTCCAAAT 
CCAGAAATAC CAGTTAGAAT ACATTTGTCC TTGGAGAAAG TACCAGATTT GATAAAAGTG 
GTTTCATCTA TTGGAGCAAT AGTGGTGGAT GGTAGAGTAT ATCCTTCAAC ATCTCCATTG 
GGTGGAATAA ATACACCTGT TATGTCATAT AATGAATCAC AAACAGATCA AAAACAAACA 
ACAAATTTGT CAAATACACA TATGGATGGT TCTTCCATAA CTGCACAATT ACCTACTACA 
ACACAACAAT CTAATAATAT GCAACAAAAT TCTTACCAAC AAGTGCCTCT TCATATAGCA 
TCTCAACAGC AACAACAAAC ACAATCGCAA AATTATGTAC AACATTATCC TATAAATAAT 
ATGCCACCTC GGTCATCACC ACAAGCTCAT CAACCACGGG TACCGTATGC AGTTAATTTC 
AACAACAGAC TACAACAACC AATAATGATA CAACAACGTC CTTTGGGAAG CTGTCATCAA 
CAAGTAACAT CATCTACGCA TCCTCATCAA CAAGTACACA TAGATCAACT TGGACAAAAT 
ACGAGTTTAC GTGGACTTCT TGCACATAAT CCACCACCTT TTCGACCTTC TACAAATCAT 
GTATCATATC GATTGCCACC TTATAGATAT CCTACAAATA ATTCTCAACG GCCAGTACCA 
CCACCTGCAT ATCAACCAAC AAATACATCA ATGGTATCTG GTATAAGACT TCAACAATGC 
AATCCTACTT CTCCATCCAC ACAACATATA AATTATCCTG TGCAACAACC AACTACAGCC 
CGTTGGCATA TACCTCAAAA TGTTAATCCT TGTCTTCCTT TTTATGCTTC CCGTCATCAA 
TCAAGTACAC CTTCCATGTC AGTTCCTAAT GATACTTACA AAATAACATT AAAAAATCAA 
CAATCAAATA CAAATCAAAA ATATAGCACA CAGACAACTA CTGCTTCTGA TAATCCACCT 
CAATCACAAA ATTCAATATC TATTGGTCAT ACCGTCAGTT CGTCAGTATC TAAAACGCCT 
AGTCCCGTGC TTCCCGGGAA ATCAGATGAA ACTGAAAAGA GTTTAGACAA ATTTTGTCAG 
AAATCTTTAA ATGACCTGAT GCTTACCATT GCTAAATTAG ATAGTAATGG AATTGTAGTT 
ATACCAGAAG CTCAACGTAA TCAATTAGAT TCTGCCCAAG TAGATAGTTC CACTGATGAA 
GGAATAAATC CAATGACTGA AAATTCATCA GATACTTCAA AAAATGCTGC AGCATCTATG 
GCAAAAGATG ATCCTAATGA AGACTGGTGT GCAGTATGTA TGGATGGAGG AGATGCAGTA 
CTTTGTTGTG ATAAATGCCC AAAAGTCTTC CATTTATATT GTCATATTCC TAGCTTAAAA 
TCATTTCCTG ATGAAAGTGA AACTTGGCAA TGTATGTTAT GTACAAATGT ATTAGATTGT 
TCAGATGATC CACCAGGAGA AAAAAGACCA AATACTATGA GTGCAAAAGA ATTGCGAATT 
GCACAAAGAA TTGTATTAGA ACTTTATTGT CAATATGAAC AAAGTTTACC TTTCCGTGAA 
GTTGTATCCA GTGAGATAGT CGACTATCAT CGCATCATAA AAAAACCAAT TGCATTAGAT 
ATAATTAGAG ATAAATTAAA ATCTGAACAT CCAAATCATT ATACAGATTT AAGACAAGTA 
ATGGCAGACA TCAGATTGAT GTTTAAAAAT GCTTTTACAT ACAATCCTGT TGAATCACAA 
GTCTATCAAG AAGCACGAAA TTTAGAAGAA TTTTTCGAAA AATTATTATT AAAATGGGCA 
CCAAATTATG CCTATGATGA TCCTTTTCTT TCAGCTGATA AAGACGAGGA TGAAGAAGTG 
TTCCCTCCTA ATAGAAAATA TCGTCGTATA GTTACAGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006613821.1
CDS	148..3249
Translation	METRTNELLTTGPVTIKEEPLDQTGVKQNMQQSQVQGVCIEEECEQRIECTTEGQETETRAFLSKCVFCGKTFTFGDDPKLLECLHAACSTCVNSKLSDHNTSVDVDVLLESNVITCQICNVTTQAENLIENRFLSKFIEEDNSPGTDDESKETEEEKKCTSCHDNANATSWCVECEEFICQNCVMAHQRLKITKDHTIKPKDEVANDRDSVKKSNKKIPGYLFCTIHSHEQLSLFCQTCDKLTCRDCQLSEHRDHKYKFIHEIAAETRASVSTLLKEVSYKRVLLKSAMKVIEDRQVLILEKKKNLVQDITQMVVQLTNTINTRGKQLVMRLNEVCDQKQNTLNEKKVALDQLSKLTDHCIQFVTHALKKGSDMELLYSKKSVTSHLQRIKSRRADIPNPEIPVRIHLSLEKVPDLIKVVSSIGAIVVDGRVYPSTSPLGGINTPVMSYNESQTDQKQTTNLSNTHMDGSSITAQLPTTTQQSNNMQQNSYQQVPLHIASQQQQQTQSQNYVQHYPINNMPPRSSPQAHQPRVPYAVNFNNRLQQPIMIQQRPLGSCHQQVTSSTHPHQQVHIDQLGQNTSLRGLLAHNPPPFRPSTNHVSYRLPPYRYPTNNSQRPVPPPAYQPTNTSMVSGIRLQQCNPTSPSTQHINYPVQQPTTARWHIPQNVNPCLPFYASRHQSSTPSMSVPNDTYKITLKNQQSNTNQKYSTQTTTASDNPPQSQNSISIGHTVSSSVSKTPSPVLPGKSDETEKSLDKFCQKSLNDLMLTIAKLDSNGIVVIPEAQRNQLDSAQVDSSTDEGINPMTENSSDTSKNAAASMAKDDPNEDWCAVCMDGGDAVLCCDKCPKVFHLYCHIPSLKSFPDESETWQCMLCTNVLDCSDDPPGEKRPNTMSAKELRIAQRIVLELYCQYEQSLPFREVVSSEIVDYHRIIKKPIALDIIRDKLKSEHPNHYTDLRQVMADIRLMFKNAFTYNPVESQVYQEARNLEEFFEKLLLKWAPNYAYDDPFLSADKDEDEEVFPPNRKYRRIVTD

Target ORF information:

RefSeq Version	XM_006613758.1
Organism	Apis dorsata(giant honeybee)
Definition	Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006613758.1

ORF Insert Sequence:

ATGGAAACTC GAACAAATGA GTTATTAACA ACAGGTCCTG TAACGATTAA AGAAGAACCA 
CTTGATCAAA CTGGTGTGAA GCAAAACATG CAACAATCTC AAGTACAGGG CGTTTGCATT 
GAAGAAGAAT GTGAACAACG TATTGAATGT ACAACTGAGG GTCAAGAGAC TGAAACACGT 
GCATTTTTAT CGAAATGTGT ATTCTGTGGA AAGACATTTA CTTTTGGTGA TGATCCAAAA 
CTTTTGGAAT GTTTACATGC AGCATGTTCG ACATGTGTCA ATAGCAAACT TAGTGATCAC 
AATACATCGG TCGACGTGGA TGTTTTGTTG GAGAGCAATG TGATTACTTG CCAAATATGT 
AATGTAACAA CACAAGCAGA AAATTTAATT GAAAATCGAT TTTTATCAAA ATTTATTGAA 
GAAGATAATA GTCCAGGTAC TGATGATGAA TCTAAAGAAA CAGAAGAAGA GAAAAAATGT 
ACTAGCTGTC ACGATAATGC TAATGCCACA AGTTGGTGTG TAGAATGTGA AGAATTTATA 
TGTCAAAATT GTGTTATGGC ACATCAAAGA TTAAAAATAA CAAAAGATCA CACAATTAAA 
CCAAAGGATG AAGTTGCCAA TGATAGAGAT AGTGTTAAAA AAAGCAACAA AAAAATACCT 
GGCTACTTAT TTTGTACAAT TCATTCACAT GAACAATTAT CTTTATTTTG TCAAACATGT 
GATAAACTTA CTTGTAGAGA TTGTCAACTA AGTGAACATA GAGATCATAA GTACAAATTT 
ATTCATGAAA TTGCTGCTGA AACCCGTGCT TCTGTATCAA CTTTACTTAA AGAAGTGAGT 
TATAAACGAG TTTTATTAAA AAGTGCAATG AAAGTTATAG AAGATAGACA AGTTCTTATA 
TTAGAGAAAA AGAAAAATTT AGTACAAGAT ATAACACAAA TGGTGGTGCA ATTAACGAAT 
ACAATTAATA CACGAGGAAA ACAATTAGTT ATGCGTTTAA ATGAAGTTTG TGATCAAAAG 
CAAAATACAT TAAATGAAAA AAAAGTTGCT TTAGATCAAT TATCAAAACT TACAGATCAT 
TGTATTCAAT TTGTAACTCA TGCTTTAAAA AAAGGTTCAG ATATGGAATT ATTATATAGT 
AAAAAATCTG TTACAAGTCA TTTACAAAGA ATAAAAAGTA GACGAGCTGA TATTCCAAAT 
CCAGAAATAC CAGTTAGAAT ACATTTGTCC TTGGAGAAAG TACCAGATTT GATAAAAGTG 
GTTTCATCTA TTGGAGCAAT AGTGGTGGAT GGTAGAGTAT ATCCTTCAAC ATCTCCATTG 
GGTGGAATAA ATACACCTGT TATGTCATAT AATGAATCAC AAACAGATCA AAAACAAACA 
ACAAATTTGT CAAATACACA TATGGATGGT TCTTCCATAA CTGCACAATT ACCTACTACA 
ACACAACAAT CTAATAATAT GCAACAAAAT TCTTACCAAC AAGTGCCTCT TCATATAGCA 
TCTCAACAGC AACAACAAAC ACAATCGCAA AATTATGTAC AACATTATCC TATAAATAAT 
ATGCCACCTC GGTCATCACC ACAAGCTCAT CAACCACGGG TACCGTATGC AGTTAATTTC 
AACAACAGAC TACAACAACC AATAATGATA CAACAACGTC CTTTGGGAAG CTGTCATCAA 
CAAGTAACAT CATCTACGCA TCCTCATCAA CAAGTACACA TAGATCAACT TGGACAAAAT 
ACGAGTTTAC GTGGACTTCT TGCACATAAT CCACCACCTT TTCGACCTTC TACAAATCAT 
GTATCATATC GATTGCCACC TTATAGATAT CCTACAAATA ATTCTCAACG GCCAGTACCA 
CCACCTGCAT ATCAACCAAC AAATACATCA ATGGTATCTG GTATAAGACT TCAACAATGC 
AATCCTACTT CTCCATCCAC ACAACATATA AATTATCCTG TGCAACAACC AACTACAGCC 
CGTTGGCATA TACCTCAAAA TGTTAATCCT TGTCTTCCTT TTTATGCTTC CCGTCATCAA 
TCAAGTACAC CTTCCATGTC AGTTCCTAAT GATACTTACA AAATAACATT AAAAAATCAA 
CAATCAAATA CAAATCAAAA ATATAGCACA CAGACAACTA CTGCTTCTGA TAATCCACCT 
CAATCACAAA ATTCAATATC TATTGGTCAT ACCGTCAGTT CGTCAGTATC TAAAACGCCT 
AGTCCCGTGC TTCCCGGGAA ATCAGATGAA ACTGAAAAGA GTTTAGACAA ATTTTGTCAG 
AAATCTTTAA ATGACCTGAT GCTTACCATT GCTAAATTAG ATAGTAATGG AATTGTAGTT 
ATACCAGAAG CTCAACGTAA TCAATTAGAT TCTGCCCAAG TAGATAGTTC CACTGATGAA 
GGAATAAATC CAATGACTGA AAATTCATCA GATACTTCAA AAAATGCTGC AGCATCTATG 
GCAAAAGATG ATCCTAATGA AGACTGGTGT GCAGTATGTA TGGATGGAGG AGATGCAGTA 
CTTTGTTGTG ATAAATGCCC AAAAGTCTTC CATTTATATT GTCATATTCC TAGCTTAAAA 
TCATTTCCTG ATGAAAGTGA AACTTGGCAA TGTATGTTAT GTACAAATGT ATTAGATTGT 
TCAGATGATC CACCAGGAGA AAAAAGACCA AATACTATGA GTGCAAAAGA ATTGCGAATT 
GCACAAAGAA TTGTATTAGA ACTTTATTGT CAATATGAAC AAAGTTTACC TTTCCGTGAA 
GTTGTATCCA GTGAGATAGT CGACTATCAT CGCATCATAA AAAAACCAAT TGCATTAGAT 
ATAATTAGAG ATAAATTAAA ATCTGAACAT CCAAATCATT ATACAGATTT AAGACAAGTA 
ATGGCAGACA TCAGATTGAT GTTTAAAAAT GCTTTTACAT ACAATCCTGT TGAATCACAA 
GTCTATCAAG AAGCACGAAA TTTAGAAGAA TTTTTCGAAA AATTATTATT AAAATGGGCA 
CCAAATTATG CCTATGATGA TCCTTTTCTT TCAGCTGATA AAGACGAGGA TGAAGAAGTG 
TTCCCTCCTA ATAGAAAATA TCGTCGTATA GTTACAGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102675864 ( XM_006613758.2 ) cDNA ORF clone, Apis dorsata(giant honeybee) -> XP_006613821.1 Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.

Price & Availability↑

CloneID	OAp66364
Clone ID Related Accession (Same CDS sequence)	XM_006613758.1 , XM_006613758.2
Accession Version	XM_006613758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Apis dorsata(giant honeybee)
Product	E3 ubiquitin-protein ligase TRIM33-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006263451.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 5, 2019 this sequence version replaced XM_006613758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis dorsata Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTC GAACAAATGA GTTATTAACA ACAGGTCCTG TAACGATTAA AGAAGAACCA 
CTTGATCAAA CTGGTGTGAA GCAAAACATG CAACAATCTC AAGTACAGGG CGTTTGCATT 
GAAGAAGAAT GTGAACAACG TATTGAATGT ACAACTGAGG GTCAAGAGAC TGAAACACGT 
GCATTTTTAT CGAAATGTGT ATTCTGTGGA AAGACATTTA CTTTTGGTGA TGATCCAAAA 
CTTTTGGAAT GTTTACATGC AGCATGTTCG ACATGTGTCA ATAGCAAACT TAGTGATCAC 
AATACATCGG TCGACGTGGA TGTTTTGTTG GAGAGCAATG TGATTACTTG CCAAATATGT 
AATGTAACAA CACAAGCAGA AAATTTAATT GAAAATCGAT TTTTATCAAA ATTTATTGAA 
GAAGATAATA GTCCAGGTAC TGATGATGAA TCTAAAGAAA CAGAAGAAGA GAAAAAATGT 
ACTAGCTGTC ACGATAATGC TAATGCCACA AGTTGGTGTG TAGAATGTGA AGAATTTATA 
TGTCAAAATT GTGTTATGGC ACATCAAAGA TTAAAAATAA CAAAAGATCA CACAATTAAA 
CCAAAGGATG AAGTTGCCAA TGATAGAGAT AGTGTTAAAA AAAGCAACAA AAAAATACCT 
GGCTACTTAT TTTGTACAAT TCATTCACAT GAACAATTAT CTTTATTTTG TCAAACATGT 
GATAAACTTA CTTGTAGAGA TTGTCAACTA AGTGAACATA GAGATCATAA GTACAAATTT 
ATTCATGAAA TTGCTGCTGA AACCCGTGCT TCTGTATCAA CTTTACTTAA AGAAGTGAGT 
TATAAACGAG TTTTATTAAA AAGTGCAATG AAAGTTATAG AAGATAGACA AGTTCTTATA 
TTAGAGAAAA AGAAAAATTT AGTACAAGAT ATAACACAAA TGGTGGTGCA ATTAACGAAT 
ACAATTAATA CACGAGGAAA ACAATTAGTT ATGCGTTTAA ATGAAGTTTG TGATCAAAAG 
CAAAATACAT TAAATGAAAA AAAAGTTGCT TTAGATCAAT TATCAAAACT TACAGATCAT 
TGTATTCAAT TTGTAACTCA TGCTTTAAAA AAAGGTTCAG ATATGGAATT ATTATATAGT 
AAAAAATCTG TTACAAGTCA TTTACAAAGA ATAAAAAGTA GACGAGCTGA TATTCCAAAT 
CCAGAAATAC CAGTTAGAAT ACATTTGTCC TTGGAGAAAG TACCAGATTT GATAAAAGTG 
GTTTCATCTA TTGGAGCAAT AGTGGTGGAT GGTAGAGTAT ATCCTTCAAC ATCTCCATTG 
GGTGGAATAA ATACACCTGT TATGTCATAT AATGAATCAC AAACAGATCA AAAACAAACA 
ACAAATTTGT CAAATACACA TATGGATGGT TCTTCCATAA CTGCACAATT ACCTACTACA 
ACACAACAAT CTAATAATAT GCAACAAAAT TCTTACCAAC AAGTGCCTCT TCATATAGCA 
TCTCAACAGC AACAACAAAC ACAATCGCAA AATTATGTAC AACATTATCC TATAAATAAT 
ATGCCACCTC GGTCATCACC ACAAGCTCAT CAACCACGGG TACCGTATGC AGTTAATTTC 
AACAACAGAC TACAACAACC AATAATGATA CAACAACGTC CTTTGGGAAG CTGTCATCAA 
CAAGTAACAT CATCTACGCA TCCTCATCAA CAAGTACACA TAGATCAACT TGGACAAAAT 
ACGAGTTTAC GTGGACTTCT TGCACATAAT CCACCACCTT TTCGACCTTC TACAAATCAT 
GTATCATATC GATTGCCACC TTATAGATAT CCTACAAATA ATTCTCAACG GCCAGTACCA 
CCACCTGCAT ATCAACCAAC AAATACATCA ATGGTATCTG GTATAAGACT TCAACAATGC 
AATCCTACTT CTCCATCCAC ACAACATATA AATTATCCTG TGCAACAACC AACTACAGCC 
CGTTGGCATA TACCTCAAAA TGTTAATCCT TGTCTTCCTT TTTATGCTTC CCGTCATCAA 
TCAAGTACAC CTTCCATGTC AGTTCCTAAT GATACTTACA AAATAACATT AAAAAATCAA 
CAATCAAATA CAAATCAAAA ATATAGCACA CAGACAACTA CTGCTTCTGA TAATCCACCT 
CAATCACAAA ATTCAATATC TATTGGTCAT ACCGTCAGTT CGTCAGTATC TAAAACGCCT 
AGTCCCGTGC TTCCCGGGAA ATCAGATGAA ACTGAAAAGA GTTTAGACAA ATTTTGTCAG 
AAATCTTTAA ATGACCTGAT GCTTACCATT GCTAAATTAG ATAGTAATGG AATTGTAGTT 
ATACCAGAAG CTCAACGTAA TCAATTAGAT TCTGCCCAAG TAGATAGTTC CACTGATGAA 
GGAATAAATC CAATGACTGA AAATTCATCA GATACTTCAA AAAATGCTGC AGCATCTATG 
GCAAAAGATG ATCCTAATGA AGACTGGTGT GCAGTATGTA TGGATGGAGG AGATGCAGTA 
CTTTGTTGTG ATAAATGCCC AAAAGTCTTC CATTTATATT GTCATATTCC TAGCTTAAAA 
TCATTTCCTG ATGAAAGTGA AACTTGGCAA TGTATGTTAT GTACAAATGT ATTAGATTGT 
TCAGATGATC CACCAGGAGA AAAAAGACCA AATACTATGA GTGCAAAAGA ATTGCGAATT 
GCACAAAGAA TTGTATTAGA ACTTTATTGT CAATATGAAC AAAGTTTACC TTTCCGTGAA 
GTTGTATCCA GTGAGATAGT CGACTATCAT CGCATCATAA AAAAACCAAT TGCATTAGAT 
ATAATTAGAG ATAAATTAAA ATCTGAACAT CCAAATCATT ATACAGATTT AAGACAAGTA 
ATGGCAGACA TCAGATTGAT GTTTAAAAAT GCTTTTACAT ACAATCCTGT TGAATCACAA 
GTCTATCAAG AAGCACGAAA TTTAGAAGAA TTTTTCGAAA AATTATTATT AAAATGGGCA 
CCAAATTATG CCTATGATGA TCCTTTTCTT TCAGCTGATA AAGACGAGGA TGAAGAAGTG 
TTCCCTCCTA ATAGAAAATA TCGTCGTATA GTTACAGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006613821.1
CDS	136..3237
Translation	METRTNELLTTGPVTIKEEPLDQTGVKQNMQQSQVQGVCIEEECEQRIECTTEGQETETRAFLSKCVFCGKTFTFGDDPKLLECLHAACSTCVNSKLSDHNTSVDVDVLLESNVITCQICNVTTQAENLIENRFLSKFIEEDNSPGTDDESKETEEEKKCTSCHDNANATSWCVECEEFICQNCVMAHQRLKITKDHTIKPKDEVANDRDSVKKSNKKIPGYLFCTIHSHEQLSLFCQTCDKLTCRDCQLSEHRDHKYKFIHEIAAETRASVSTLLKEVSYKRVLLKSAMKVIEDRQVLILEKKKNLVQDITQMVVQLTNTINTRGKQLVMRLNEVCDQKQNTLNEKKVALDQLSKLTDHCIQFVTHALKKGSDMELLYSKKSVTSHLQRIKSRRADIPNPEIPVRIHLSLEKVPDLIKVVSSIGAIVVDGRVYPSTSPLGGINTPVMSYNESQTDQKQTTNLSNTHMDGSSITAQLPTTTQQSNNMQQNSYQQVPLHIASQQQQQTQSQNYVQHYPINNMPPRSSPQAHQPRVPYAVNFNNRLQQPIMIQQRPLGSCHQQVTSSTHPHQQVHIDQLGQNTSLRGLLAHNPPPFRPSTNHVSYRLPPYRYPTNNSQRPVPPPAYQPTNTSMVSGIRLQQCNPTSPSTQHINYPVQQPTTARWHIPQNVNPCLPFYASRHQSSTPSMSVPNDTYKITLKNQQSNTNQKYSTQTTTASDNPPQSQNSISIGHTVSSSVSKTPSPVLPGKSDETEKSLDKFCQKSLNDLMLTIAKLDSNGIVVIPEAQRNQLDSAQVDSSTDEGINPMTENSSDTSKNAAASMAKDDPNEDWCAVCMDGGDAVLCCDKCPKVFHLYCHIPSLKSFPDESETWQCMLCTNVLDCSDDPPGEKRPNTMSAKELRIAQRIVLELYCQYEQSLPFREVVSSEIVDYHRIIKKPIALDIIRDKLKSEHPNHYTDLRQVMADIRLMFKNAFTYNPVESQVYQEARNLEEFFEKLLLKWAPNYAYDDPFLSADKDEDEEVFPPNRKYRRIVTD

Target ORF information:

RefSeq Version	XM_006613758.2
Organism	Apis dorsata(giant honeybee)
Definition	Apis dorsata E3 ubiquitin-protein ligase TRIM33-like (LOC102675864), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006613758.2

ORF Insert Sequence:

ATGGAAACTC GAACAAATGA GTTATTAACA ACAGGTCCTG TAACGATTAA AGAAGAACCA 
CTTGATCAAA CTGGTGTGAA GCAAAACATG CAACAATCTC AAGTACAGGG CGTTTGCATT 
GAAGAAGAAT GTGAACAACG TATTGAATGT ACAACTGAGG GTCAAGAGAC TGAAACACGT 
GCATTTTTAT CGAAATGTGT ATTCTGTGGA AAGACATTTA CTTTTGGTGA TGATCCAAAA 
CTTTTGGAAT GTTTACATGC AGCATGTTCG ACATGTGTCA ATAGCAAACT TAGTGATCAC 
AATACATCGG TCGACGTGGA TGTTTTGTTG GAGAGCAATG TGATTACTTG CCAAATATGT 
AATGTAACAA CACAAGCAGA AAATTTAATT GAAAATCGAT TTTTATCAAA ATTTATTGAA 
GAAGATAATA GTCCAGGTAC TGATGATGAA TCTAAAGAAA CAGAAGAAGA GAAAAAATGT 
ACTAGCTGTC ACGATAATGC TAATGCCACA AGTTGGTGTG TAGAATGTGA AGAATTTATA 
TGTCAAAATT GTGTTATGGC ACATCAAAGA TTAAAAATAA CAAAAGATCA CACAATTAAA 
CCAAAGGATG AAGTTGCCAA TGATAGAGAT AGTGTTAAAA AAAGCAACAA AAAAATACCT 
GGCTACTTAT TTTGTACAAT TCATTCACAT GAACAATTAT CTTTATTTTG TCAAACATGT 
GATAAACTTA CTTGTAGAGA TTGTCAACTA AGTGAACATA GAGATCATAA GTACAAATTT 
ATTCATGAAA TTGCTGCTGA AACCCGTGCT TCTGTATCAA CTTTACTTAA AGAAGTGAGT 
TATAAACGAG TTTTATTAAA AAGTGCAATG AAAGTTATAG AAGATAGACA AGTTCTTATA 
TTAGAGAAAA AGAAAAATTT AGTACAAGAT ATAACACAAA TGGTGGTGCA ATTAACGAAT 
ACAATTAATA CACGAGGAAA ACAATTAGTT ATGCGTTTAA ATGAAGTTTG TGATCAAAAG 
CAAAATACAT TAAATGAAAA AAAAGTTGCT TTAGATCAAT TATCAAAACT TACAGATCAT 
TGTATTCAAT TTGTAACTCA TGCTTTAAAA AAAGGTTCAG ATATGGAATT ATTATATAGT 
AAAAAATCTG TTACAAGTCA TTTACAAAGA ATAAAAAGTA GACGAGCTGA TATTCCAAAT 
CCAGAAATAC CAGTTAGAAT ACATTTGTCC TTGGAGAAAG TACCAGATTT GATAAAAGTG 
GTTTCATCTA TTGGAGCAAT AGTGGTGGAT GGTAGAGTAT ATCCTTCAAC ATCTCCATTG 
GGTGGAATAA ATACACCTGT TATGTCATAT AATGAATCAC AAACAGATCA AAAACAAACA 
ACAAATTTGT CAAATACACA TATGGATGGT TCTTCCATAA CTGCACAATT ACCTACTACA 
ACACAACAAT CTAATAATAT GCAACAAAAT TCTTACCAAC AAGTGCCTCT TCATATAGCA 
TCTCAACAGC AACAACAAAC ACAATCGCAA AATTATGTAC AACATTATCC TATAAATAAT 
ATGCCACCTC GGTCATCACC ACAAGCTCAT CAACCACGGG TACCGTATGC AGTTAATTTC 
AACAACAGAC TACAACAACC AATAATGATA CAACAACGTC CTTTGGGAAG CTGTCATCAA 
CAAGTAACAT CATCTACGCA TCCTCATCAA CAAGTACACA TAGATCAACT TGGACAAAAT 
ACGAGTTTAC GTGGACTTCT TGCACATAAT CCACCACCTT TTCGACCTTC TACAAATCAT 
GTATCATATC GATTGCCACC TTATAGATAT CCTACAAATA ATTCTCAACG GCCAGTACCA 
CCACCTGCAT ATCAACCAAC AAATACATCA ATGGTATCTG GTATAAGACT TCAACAATGC 
AATCCTACTT CTCCATCCAC ACAACATATA AATTATCCTG TGCAACAACC AACTACAGCC 
CGTTGGCATA TACCTCAAAA TGTTAATCCT TGTCTTCCTT TTTATGCTTC CCGTCATCAA 
TCAAGTACAC CTTCCATGTC AGTTCCTAAT GATACTTACA AAATAACATT AAAAAATCAA 
CAATCAAATA CAAATCAAAA ATATAGCACA CAGACAACTA CTGCTTCTGA TAATCCACCT 
CAATCACAAA ATTCAATATC TATTGGTCAT ACCGTCAGTT CGTCAGTATC TAAAACGCCT 
AGTCCCGTGC TTCCCGGGAA ATCAGATGAA ACTGAAAAGA GTTTAGACAA ATTTTGTCAG 
AAATCTTTAA ATGACCTGAT GCTTACCATT GCTAAATTAG ATAGTAATGG AATTGTAGTT 
ATACCAGAAG CTCAACGTAA TCAATTAGAT TCTGCCCAAG TAGATAGTTC CACTGATGAA 
GGAATAAATC CAATGACTGA AAATTCATCA GATACTTCAA AAAATGCTGC AGCATCTATG 
GCAAAAGATG ATCCTAATGA AGACTGGTGT GCAGTATGTA TGGATGGAGG AGATGCAGTA 
CTTTGTTGTG ATAAATGCCC AAAAGTCTTC CATTTATATT GTCATATTCC TAGCTTAAAA 
TCATTTCCTG ATGAAAGTGA AACTTGGCAA TGTATGTTAT GTACAAATGT ATTAGATTGT 
TCAGATGATC CACCAGGAGA AAAAAGACCA AATACTATGA GTGCAAAAGA ATTGCGAATT 
GCACAAAGAA TTGTATTAGA ACTTTATTGT CAATATGAAC AAAGTTTACC TTTCCGTGAA 
GTTGTATCCA GTGAGATAGT CGACTATCAT CGCATCATAA AAAAACCAAT TGCATTAGAT 
ATAATTAGAG ATAAATTAAA ATCTGAACAT CCAAATCATT ATACAGATTT AAGACAAGTA 
ATGGCAGACA TCAGATTGAT GTTTAAAAAT GCTTTTACAT ACAATCCTGT TGAATCACAA 
GTCTATCAAG AAGCACGAAA TTTAGAAGAA TTTTTCGAAA AATTATTATT AAAATGGGCA 
CCAAATTATG CCTATGATGA TCCTTTTCTT TCAGCTGATA AAGACGAGGA TGAAGAAGTG 
TTCCCTCCTA ATAGAAAATA TCGTCGTATA GTTACAGATT AA