LOC103280010 cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103280010
Entrez Gene ID	103280010
Full Name	interleukin-27 subunit alpha
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008117521.2	XP_008115728.1	interleukin-27 subunit alpha isoform X2
XM_008117520.2	XP_008115727.1	interleukin-27 subunit alpha isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC103280010 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103280010 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn13597	XM_008117520.2 Latest version!	Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OAn13598	XM_008117521.2 Latest version!	Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103280010 ( XM_008117520.2 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_008115727.1 Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn13597
Clone ID Related Accession (Same CDS sequence)	XM_008117520.2
Accession Version	XM_008117520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	interleukin-27 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003338834.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_008117520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATAA AGTTTCTCCC TTCTCATGAC TATCTGGATC TCTTTCTAGG TGCAGCCTTC 
CTCCTTCAGA TTCTCTTCAG TGTTGACCTC CTTGTGGCAG CTGTCCCGGT TGGGCTCCGT 
GGGGAGAAGG CGTCAGGGCT CAAGAGCAGC TCAGAATGGA GAGCAAACCT TCAGAAAGAA 
TTGGGCAGCA GCTTAAAGCT CTCAAGGCAA CTTCTCTGTA AAACAAGAAA CCTTAAACAT 
CTCTATCAAT CCGAACGTCT GCCTGGAGCC CACCTCTTTC TTATACCTCA CTCTCGGCGG 
CTTCCCTCAC CCAACCTTAA TCTGTCTACG TGGCTAGCTC TTCCGGATGC CAAGCGCTTG 
TCCCACATGG CTGAGGGGCT CTCCTTCTAT CAGACTTTGG TACAGCAGCT CAGGGATCAT 
GAAGCCACCA AAGAAGATTT CAGACTTCTT TCCCAGCTTG AAGATCTCCG CTGTAATCTC 
CGAGACTTAA GTCATCACGT AAGCTATCAG ATTTCACGTT GGGAGCTGCC ATTTGAAAAC 
CACCTTGAGC CAGCTCTCCA GCCTCCTCAG ATCCTTCAGC ATCAGAATCA GTGGAGCAAC 
CGGCAAGAAG TGTACCTCGT CCTGCGATAT CTGGAGAATT TCCTGTGTCG TGTCACAAGA 
GATTTTGTGA TGCTCAGAGC TAGACTGGTG AAGGGAACTT TCCTTTCCAG AGGTCCCCGA 
ACTCCCAGTT CCCATTCGAT CTCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008115727.1
CDS	564..1313
Translation	MTIKFLPSHDYLDLFLGAAFLLQILFSVDLLVAAVPVGLRGEKASGLKSSSEWRANLQKELGSSLKLSRQLLCKTRNLKHLYQSERLPGAHLFLIPHSRRLPSPNLNLSTWLALPDAKRLSHMAEGLSFYQTLVQQLRDHEATKEDFRLLSQLEDLRCNLRDLSHHVSYQISRWELPFENHLEPALQPPQILQHQNQWSNRQEVYLVLRYLENFLCRVTRDFVMLRARLVKGTFLSRGPRTPSSHSISS

Target ORF information:

RefSeq Version	XM_008117520.2
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008117520.2

ORF Insert Sequence:

ATGACAATAA AGTTTCTCCC TTCTCATGAC TATCTGGATC TCTTTCTAGG TGCAGCCTTC 
CTCCTTCAGA TTCTCTTCAG TGTTGACCTC CTTGTGGCAG CTGTCCCGGT TGGGCTCCGT 
GGGGAGAAGG CGTCAGGGCT CAAGAGCAGC TCAGAATGGA GAGCAAACCT TCAGAAAGAA 
TTGGGCAGCA GCTTAAAGCT CTCAAGGCAA CTTCTCTGTA AAACAAGAAA CCTTAAACAT 
CTCTATCAAT CCGAACGTCT GCCTGGAGCC CACCTCTTTC TTATACCTCA CTCTCGGCGG 
CTTCCCTCAC CCAACCTTAA TCTGTCTACG TGGCTAGCTC TTCCGGATGC CAAGCGCTTG 
TCCCACATGG CTGAGGGGCT CTCCTTCTAT CAGACTTTGG TACAGCAGCT CAGGGATCAT 
GAAGCCACCA AAGAAGATTT CAGACTTCTT TCCCAGCTTG AAGATCTCCG CTGTAATCTC 
CGAGACTTAA GTCATCACGT AAGCTATCAG ATTTCACGTT GGGAGCTGCC ATTTGAAAAC 
CACCTTGAGC CAGCTCTCCA GCCTCCTCAG ATCCTTCAGC ATCAGAATCA GTGGAGCAAC 
CGGCAAGAAG TGTACCTCGT CCTGCGATAT CTGGAGAATT TCCTGTGTCG TGTCACAAGA 
GATTTTGTGA TGCTCAGAGC TAGACTGGTG AAGGGAACTT TCCTTTCCAG AGGTCCCCGA 
ACTCCCAGTT CCCATTCGAT CTCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103280010 ( XM_008117521.2 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_008115728.1 Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn13598
Clone ID Related Accession (Same CDS sequence)	XM_008117521.2
Accession Version	XM_008117521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	interleukin-27 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003338834.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_008117521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCTGT GTGGTGCAGC CTTCCTCCTT CAGATTCTCT TCAGTGTTGA CCTCCTTGTG 
GCAGCTGTCC CGGTTGGGCT CCGTGGGGAG AAGGCGTCAG GGCTCAAGAG CAGCTCAGAA 
TGGAGAGCAA ACCTTCAGAA AGAATTGGGC AGCAGCTTAA AGCTCTCAAG GCAACTTCTC 
TGTAAAACAA GAAACCTTAA ACATCTCTAT CAATCCGAAC GTCTGCCTGG AGCCCACCTC 
TTTCTTATAC CTCACTCTCG GCGGCTTCCC TCACCCAACC TTAATCTGTC TACGTGGCTA 
GCTCTTCCGG ATGCCAAGCG CTTGTCCCAC ATGGCTGAGG GGCTCTCCTT CTATCAGACT 
TTGGTACAGC AGCTCAGGGA TCATGAAGCC ACCAAAGAAG ATTTCAGACT TCTTTCCCAG 
CTTGAAGATC TCCGCTGTAA TCTCCGAGAC TTAAGTCATC ACGTAAGCTA TCAGATTTCA 
CGTTGGGAGC TGCCATTTGA AAACCACCTT GAGCCAGCTC TCCAGCCTCC TCAGATCCTT 
CAGCATCAGA ATCAGTGGAG CAACCGGCAA GAAGTGTACC TCGTCCTGCG ATATCTGGAG 
AATTTCCTGT GTCGTGTCAC AAGAGATTTT GTGATGCTCA GAGCTAGACT GGTGAAGGGA 
ACTTTCCTTT CCAGAGGTCC CCGAACTCCC AGTTCCCATT CGATCTCATC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008115728.1
CDS	357..1070
Translation	MRLCGAAFLLQILFSVDLLVAAVPVGLRGEKASGLKSSSEWRANLQKELGSSLKLSRQLLCKTRNLKHLYQSERLPGAHLFLIPHSRRLPSPNLNLSTWLALPDAKRLSHMAEGLSFYQTLVQQLRDHEATKEDFRLLSQLEDLRCNLRDLSHHVSYQISRWELPFENHLEPALQPPQILQHQNQWSNRQEVYLVLRYLENFLCRVTRDFVMLRARLVKGTFLSRGPRTPSSHSISS

Target ORF information:

RefSeq Version	XM_008117521.2
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis interleukin-27 subunit alpha (LOC103280010), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008117521.2

ORF Insert Sequence:

ATGCGTCTGT GTGGTGCAGC CTTCCTCCTT CAGATTCTCT TCAGTGTTGA CCTCCTTGTG 
GCAGCTGTCC CGGTTGGGCT CCGTGGGGAG AAGGCGTCAG GGCTCAAGAG CAGCTCAGAA 
TGGAGAGCAA ACCTTCAGAA AGAATTGGGC AGCAGCTTAA AGCTCTCAAG GCAACTTCTC 
TGTAAAACAA GAAACCTTAA ACATCTCTAT CAATCCGAAC GTCTGCCTGG AGCCCACCTC 
TTTCTTATAC CTCACTCTCG GCGGCTTCCC TCACCCAACC TTAATCTGTC TACGTGGCTA 
GCTCTTCCGG ATGCCAAGCG CTTGTCCCAC ATGGCTGAGG GGCTCTCCTT CTATCAGACT 
TTGGTACAGC AGCTCAGGGA TCATGAAGCC ACCAAAGAAG ATTTCAGACT TCTTTCCCAG 
CTTGAAGATC TCCGCTGTAA TCTCCGAGAC TTAAGTCATC ACGTAAGCTA TCAGATTTCA 
CGTTGGGAGC TGCCATTTGA AAACCACCTT GAGCCAGCTC TCCAGCCTCC TCAGATCCTT 
CAGCATCAGA ATCAGTGGAG CAACCGGCAA GAAGTGTACC TCGTCCTGCG ATATCTGGAG 
AATTTCCTGT GTCGTGTCAC AAGAGATTTT GTGATGCTCA GAGCTAGACT GGTGAAGGGA 
ACTTTCCTTT CCAGAGGTCC CCGAACTCCC AGTTCCCATT CGATCTCATC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.