LOC109712834 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109712834
Entrez Gene ID	109712834
Full Name	leucine--tRNA ligase, cytoplasmic-like
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020236616.1	XP_020092205.1	leucine--tRNA ligase, cytoplasmic-like isoform X2
XM_020236608.1	XP_020092197.1	leucine--tRNA ligase, cytoplasmic-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109712834 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109712834 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn58867	XM_020236608.1 Latest version!	Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAn58868	XM_020236616.1 Latest version!	Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109712834 ( XM_020236608.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020092197.1 Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn58867
Clone ID Related Accession (Same CDS sequence)	XM_020236608.1
Accession Version	XM_020236608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	leucine--tRNA ligase, cytoplasmic-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCGA TAAAGTTTAG ATTTTTCAGA AAAATGTCGA CGAATACTGA GGGAGGAGGA 
AGGAGCTATG CTCGGCGAGA CCTACTTCTC AAGATACAAT CAGAAATCCA AAATTACTGG 
GAAGAAAACA AAATCTTCGA GGCCGAACCC GGTGTTAAAC CTCCTGAGCC GGGCGAGAAG 
TTCTTCGGCA ACTTCCCATA CCCCTACATG AATGGCCTGC TCCATTTGGG CCACGCCTTC 
ACTCTTTCTA AGCTTGAGTT TGGAGCCGCC TACCACCGCC TACGTGGCTG TAACGTTCTA 
TTGCCTTTCG CTTTCCACTG CACGGGAATG CCTATCAAAG CATCGGCCGA CAAACTTAAA 
AGAGAAATCG AACAGCATGG AAACCCTCCT GTTTTCCCCT CATTAGAGGA GTTGACTATT 
GAAGTTGAAG AGAGTGATAA TGTGTCTGCG GCTACGCCCG ATAAGTTTAA GAGCAAGAGG 
TCTAAAGCTG CTGCTAAGTC TAGCGGGGAT AAGTCGCAGT GGGAAATTAT GAGAAGTTTC 
GGCCTTTCCG ACGAAGAGAT CGCTAAGTTT CAAGACCCGT ATCATTGGCT TAAACACTTC 
CCCCCGCTGG CTAAAGAGGA TCTCAAGGCC TTTGGGCTAG GCTGCGATTG GAGGCGCTCT 
TTTATAACGA CTGACATGAA CCCGTTCTAC GATTCCTTTG TTAGGTGGCA GATGAATAAG 
TTAAAGAACA TGGGTAAGAT CGTAAAGGAT ATGAGATACA CTATTTATTC ACCGTTGGAT 
GGCCAACCTT GCGCTGATCA CGATAGAGCC TCTGGCGAAG GAGTTCAACC TCAAGAATAT 
GTATTGATTA AGATGGAGGT GATCCCTCCT TTTCCAGCCA AGTTGAAGGC TTTGGAGGGT 
AGAAAAGTCT TCTTGGCGGC AGCTACGTTG AGGCCCGAGA CAATGTATGG GCAGACTAAC 
TGTTGGGTAT TGCCGGATGG GAATTACGGA GCTTTCGAGA TCAATGATAC CGACGTTTTC 
ATCCTCACTG AGAGGGCTGC GTTGAACTTA GCTTATCAGA AGCTGTCGAA AGTCCCTGAG 
AAGCTGAGCT GTTTGCTTAA GCTCTCTGGT CATGACCTTA TTGGCTTGCC GTTAAGTTCT 
CCTCTTGCTT TTAATGAAGT GATATACTCA CTTCCTATGC TAACTATATT AACTGATAAG 
GGTACGGGCA TTGTTACGAG TGTTCCGAGT GACTCGCCAG ATGACTACAT GGCTTTAAAG 
GATTTAGTAT CTAAACCGGC CTTACGAGAA AAGTATGGCG TTAAAGATGA GTGGGTTCTG 
CCTTATGAGG TCGTGCCCAT AATAAATATT CCGGAGTTCG GAGATAAATC GGCGGAAAAG 
GTGTGTATCG ATCTTAAGAT CAAGAGCCAG AATGATAAGG AGAAACTCGC AGAGGCGAAG 
AGACTGACTT ATTTGAAAGG CTTTACGGAT GGTAGTATGT TAGTAGGAGA ATTCCAAGGG 
AGAAAGGTTC AAGAAGCGAA GCCGCTTATA AAGAACAAAT TGTTGGAAGC TGGAATGGCC 
GTGCTGTACA GTGAGCCTGA AAAGAAAGTT ATGTCGAGAT CCGGTGATGA GTGTGTCGTG 
GCGCTTACGG ATCAATGGTA CATAACTTAT GGGGAGGTGG AGTGGAAGGA GAAGGCAATG 
GAGTGCCTAG AGAAAATGAA TACTTTTTCT ACCGAGACCC GAAATGGCTT CGAGCATACA 
TTGAGCTGGC TAAATCAGTG GGCTTGCTCA CGGTCTTTCG GGCTCGGGAC TCGAATTCCT 
TGGGACGAGC AGTTTCTCGT CGAATCGCTT TCTGATTCGA CCCTTTACAT GGCTTATTAC 
ACTATTGCGC ATGTCTTGCA AAATGGCGAT ATGTATGGAT CAAATGCTTC TTCAGTTAGA 
CCAGAGCAGA TGACAGATGA GGTATGGGAT TATATCTTCT GCGACGGCCC AGTTCCGAAA 
TCTGAGATTT CTCCTGCTCT TCTAAGTAAA ATGAAGAAAG AATTCGAGTA TTGGTATCCG 
TTTGATCTTA GGGTTTCAGG GAAAGATCTC ATTCAGAACC ATCTAACATT TTGCATATAT 
AACCATACAG CGCTTCTCCC GAAGCACTTA TGGCCCCGAG GGTTCCGTTG CAACGGGCAT 
CTTATGCTTA ACTCCGAGAA AATGTCCAAG TCTACCGGAA ACTTCCGCAC ACTTCGCCAG 
GCCATAGAGG AATTCTCCTC TGACGCCACT CGGTTCTCTC TCGCAGATGC TGGCGACGGG 
ATGGACGATG CGAACTTCGT CTTCGAGACA GCAAATGCCG CGATTTTGAG GCTGACTAAA 
GAAATTGCCT GGATGGAAGA GGTGCTTTCT GTCGAGTCTT CTTTGCGAGC CGGCCCCCCA 
TCGTCTTATG CTGACCGGGT TTTCGCCAAT GAGATGAATT TCGCGGTGGT GGAAACCGAG 
AAAAGTTACA ACAACTTCAT GTTTAGAGAA GCTTTGAAAA CCGGGTTTTA CGACCTTCAA 
GCTGCGAGAG ATGAGTACAG ATTTTCGTGC GGGGCGGGAG GTATGAATAA GGAATTGTTG 
TTCCGGTTTA TGGATGTTCA GACCAGGCTT ATCACCCCGA TTTGTCCACA TTACGCGGAA 
CATGTGTGGA AAAATATATT GAAAAATGAC GGGTTTATAG TAAGAGCCGG TTGGCCATTT 
GCAGAACCCC CGAATCAAAC GCTACGAATC GCCAACAAGT ATTTACAGGA CTCTATTGTT 
TTGATGAGAA AGCTGCTTCA GAAGCAGGAA TCGGGTCCGA AAAAGGCTAA GAAGGGTGCT 
TCTGCTGCTC CTGCTGAAGA GAACAAACTG ACCGTCGGGC TTATTTTCGT GAACGAGCGA 
TACGATGGTT GGAAAGAAGA ATGCTTGAGG ATACTACGAA GCAAATTCGA TGACACAACG 
GGTTCTTTTG CTCCCGACAA AGAGATTCTC GAGGCGCTAA AATCAAGTTC TATTGGAAAT 
GATGCTAATT TTAAGCAGAT ACAGAAGCTC TGCATGCCTT TCATAAAGTT TAAGAAGGAC 
GAGACGAAGG AAGTGGGTGT TCGCGCTTTA GATTTGAAGC TACCGTTTGG CGAAATCGAG 
GTTCTCAAGG AGAATGTCGA CGTGATAAAG AGGCAGTTGG GTCTCGAGCA CGTGGAAGTT 
TTATGCGCAA CGGACGATTC CGATCGCAGT AAATCCGGAC CGCACATCTC GTTGCTGACG 
CAGAATCCGC TGTCTCCTGG TAATCCGGTT GCCATTTTCC TCAGCAAAGC AGAATTTTCG 
GCAACCGGAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020092197.1
CDS	65..3379
Translation	MNSIKFRFFRKMSTNTEGGGRSYARRDLLLKIQSEIQNYWEENKIFEAEPGVKPPEPGEKFFGNFPYPYMNGLLHLGHAFTLSKLEFGAAYHRLRGCNVLLPFAFHCTGMPIKASADKLKREIEQHGNPPVFPSLEELTIEVEESDNVSAATPDKFKSKRSKAAAKSSGDKSQWEIMRSFGLSDEEIAKFQDPYHWLKHFPPLAKEDLKAFGLGCDWRRSFITTDMNPFYDSFVRWQMNKLKNMGKIVKDMRYTIYSPLDGQPCADHDRASGEGVQPQEYVLIKMEVIPPFPAKLKALEGRKVFLAAATLRPETMYGQTNCWVLPDGNYGAFEINDTDVFILTERAALNLAYQKLSKVPEKLSCLLKLSGHDLIGLPLSSPLAFNEVIYSLPMLTILTDKGTGIVTSVPSDSPDDYMALKDLVSKPALREKYGVKDEWVLPYEVVPIINIPEFGDKSAEKVCIDLKIKSQNDKEKLAEAKRLTYLKGFTDGSMLVGEFQGRKVQEAKPLIKNKLLEAGMAVLYSEPEKKVMSRSGDECVVALTDQWYITYGEVEWKEKAMECLEKMNTFSTETRNGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTLYMAYYTIAHVLQNGDMYGSNASSVRPEQMTDEVWDYIFCDGPVPKSEISPALLSKMKKEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTALLPKHLWPRGFRCNGHLMLNSEKMSKSTGNFRTLRQAIEEFSSDATRFSLADAGDGMDDANFVFETANAAILRLTKEIAWMEEVLSVESSLRAGPPSSYADRVFANEMNFAVVETEKSYNNFMFREALKTGFYDLQAARDEYRFSCGAGGMNKELLFRFMDVQTRLITPICPHYAEHVWKNILKNDGFIVRAGWPFAEPPNQTLRIANKYLQDSIVLMRKLLQKQESGPKKAKKGASAAPAEENKLTVGLIFVNERYDGWKEECLRILRSKFDDTTGSFAPDKEILEALKSSSIGNDANFKQIQKLCMPFIKFKKDETKEVGVRALDLKLPFGEIEVLKENVDVIKRQLGLEHVEVLCATDDSDRSKSGPHISLLTQNPLSPGNPVAIFLSKAEFSATGY

Target ORF information:

RefSeq Version	XM_020236608.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236608.1

ORF Insert Sequence:

ATGAATTCGA TAAAGTTTAG ATTTTTCAGA AAAATGTCGA CGAATACTGA GGGAGGAGGA 
AGGAGCTATG CTCGGCGAGA CCTACTTCTC AAGATACAAT CAGAAATCCA AAATTACTGG 
GAAGAAAACA AAATCTTCGA GGCCGAACCC GGTGTTAAAC CTCCTGAGCC GGGCGAGAAG 
TTCTTCGGCA ACTTCCCATA CCCCTACATG AATGGCCTGC TCCATTTGGG CCACGCCTTC 
ACTCTTTCTA AGCTTGAGTT TGGAGCCGCC TACCACCGCC TACGTGGCTG TAACGTTCTA 
TTGCCTTTCG CTTTCCACTG CACGGGAATG CCTATCAAAG CATCGGCCGA CAAACTTAAA 
AGAGAAATCG AACAGCATGG AAACCCTCCT GTTTTCCCCT CATTAGAGGA GTTGACTATT 
GAAGTTGAAG AGAGTGATAA TGTGTCTGCG GCTACGCCCG ATAAGTTTAA GAGCAAGAGG 
TCTAAAGCTG CTGCTAAGTC TAGCGGGGAT AAGTCGCAGT GGGAAATTAT GAGAAGTTTC 
GGCCTTTCCG ACGAAGAGAT CGCTAAGTTT CAAGACCCGT ATCATTGGCT TAAACACTTC 
CCCCCGCTGG CTAAAGAGGA TCTCAAGGCC TTTGGGCTAG GCTGCGATTG GAGGCGCTCT 
TTTATAACGA CTGACATGAA CCCGTTCTAC GATTCCTTTG TTAGGTGGCA GATGAATAAG 
TTAAAGAACA TGGGTAAGAT CGTAAAGGAT ATGAGATACA CTATTTATTC ACCGTTGGAT 
GGCCAACCTT GCGCTGATCA CGATAGAGCC TCTGGCGAAG GAGTTCAACC TCAAGAATAT 
GTATTGATTA AGATGGAGGT GATCCCTCCT TTTCCAGCCA AGTTGAAGGC TTTGGAGGGT 
AGAAAAGTCT TCTTGGCGGC AGCTACGTTG AGGCCCGAGA CAATGTATGG GCAGACTAAC 
TGTTGGGTAT TGCCGGATGG GAATTACGGA GCTTTCGAGA TCAATGATAC CGACGTTTTC 
ATCCTCACTG AGAGGGCTGC GTTGAACTTA GCTTATCAGA AGCTGTCGAA AGTCCCTGAG 
AAGCTGAGCT GTTTGCTTAA GCTCTCTGGT CATGACCTTA TTGGCTTGCC GTTAAGTTCT 
CCTCTTGCTT TTAATGAAGT GATATACTCA CTTCCTATGC TAACTATATT AACTGATAAG 
GGTACGGGCA TTGTTACGAG TGTTCCGAGT GACTCGCCAG ATGACTACAT GGCTTTAAAG 
GATTTAGTAT CTAAACCGGC CTTACGAGAA AAGTATGGCG TTAAAGATGA GTGGGTTCTG 
CCTTATGAGG TCGTGCCCAT AATAAATATT CCGGAGTTCG GAGATAAATC GGCGGAAAAG 
GTGTGTATCG ATCTTAAGAT CAAGAGCCAG AATGATAAGG AGAAACTCGC AGAGGCGAAG 
AGACTGACTT ATTTGAAAGG CTTTACGGAT GGTAGTATGT TAGTAGGAGA ATTCCAAGGG 
AGAAAGGTTC AAGAAGCGAA GCCGCTTATA AAGAACAAAT TGTTGGAAGC TGGAATGGCC 
GTGCTGTACA GTGAGCCTGA AAAGAAAGTT ATGTCGAGAT CCGGTGATGA GTGTGTCGTG 
GCGCTTACGG ATCAATGGTA CATAACTTAT GGGGAGGTGG AGTGGAAGGA GAAGGCAATG 
GAGTGCCTAG AGAAAATGAA TACTTTTTCT ACCGAGACCC GAAATGGCTT CGAGCATACA 
TTGAGCTGGC TAAATCAGTG GGCTTGCTCA CGGTCTTTCG GGCTCGGGAC TCGAATTCCT 
TGGGACGAGC AGTTTCTCGT CGAATCGCTT TCTGATTCGA CCCTTTACAT GGCTTATTAC 
ACTATTGCGC ATGTCTTGCA AAATGGCGAT ATGTATGGAT CAAATGCTTC TTCAGTTAGA 
CCAGAGCAGA TGACAGATGA GGTATGGGAT TATATCTTCT GCGACGGCCC AGTTCCGAAA 
TCTGAGATTT CTCCTGCTCT TCTAAGTAAA ATGAAGAAAG AATTCGAGTA TTGGTATCCG 
TTTGATCTTA GGGTTTCAGG GAAAGATCTC ATTCAGAACC ATCTAACATT TTGCATATAT 
AACCATACAG CGCTTCTCCC GAAGCACTTA TGGCCCCGAG GGTTCCGTTG CAACGGGCAT 
CTTATGCTTA ACTCCGAGAA AATGTCCAAG TCTACCGGAA ACTTCCGCAC ACTTCGCCAG 
GCCATAGAGG AATTCTCCTC TGACGCCACT CGGTTCTCTC TCGCAGATGC TGGCGACGGG 
ATGGACGATG CGAACTTCGT CTTCGAGACA GCAAATGCCG CGATTTTGAG GCTGACTAAA 
GAAATTGCCT GGATGGAAGA GGTGCTTTCT GTCGAGTCTT CTTTGCGAGC CGGCCCCCCA 
TCGTCTTATG CTGACCGGGT TTTCGCCAAT GAGATGAATT TCGCGGTGGT GGAAACCGAG 
AAAAGTTACA ACAACTTCAT GTTTAGAGAA GCTTTGAAAA CCGGGTTTTA CGACCTTCAA 
GCTGCGAGAG ATGAGTACAG ATTTTCGTGC GGGGCGGGAG GTATGAATAA GGAATTGTTG 
TTCCGGTTTA TGGATGTTCA GACCAGGCTT ATCACCCCGA TTTGTCCACA TTACGCGGAA 
CATGTGTGGA AAAATATATT GAAAAATGAC GGGTTTATAG TAAGAGCCGG TTGGCCATTT 
GCAGAACCCC CGAATCAAAC GCTACGAATC GCCAACAAGT ATTTACAGGA CTCTATTGTT 
TTGATGAGAA AGCTGCTTCA GAAGCAGGAA TCGGGTCCGA AAAAGGCTAA GAAGGGTGCT 
TCTGCTGCTC CTGCTGAAGA GAACAAACTG ACCGTCGGGC TTATTTTCGT GAACGAGCGA 
TACGATGGTT GGAAAGAAGA ATGCTTGAGG ATACTACGAA GCAAATTCGA TGACACAACG 
GGTTCTTTTG CTCCCGACAA AGAGATTCTC GAGGCGCTAA AATCAAGTTC TATTGGAAAT 
GATGCTAATT TTAAGCAGAT ACAGAAGCTC TGCATGCCTT TCATAAAGTT TAAGAAGGAC 
GAGACGAAGG AAGTGGGTGT TCGCGCTTTA GATTTGAAGC TACCGTTTGG CGAAATCGAG 
GTTCTCAAGG AGAATGTCGA CGTGATAAAG AGGCAGTTGG GTCTCGAGCA CGTGGAAGTT 
TTATGCGCAA CGGACGATTC CGATCGCAGT AAATCCGGAC CGCACATCTC GTTGCTGACG 
CAGAATCCGC TGTCTCCTGG TAATCCGGTT GCCATTTTCC TCAGCAAAGC AGAATTTTCG 
GCAACCGGAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109712834 ( XM_020236616.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020092205.1 Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn58868
Clone ID Related Accession (Same CDS sequence)	XM_020236616.1
Accession Version	XM_020236616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	leucine--tRNA ligase, cytoplasmic-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGA ATACTGAGGG AGGAGGAAGG AGCTATGCTC GGCGAGACCT ACTTCTCAAG 
ATACAATCAG AAATCCAAAA TTACTGGGAA GAAAACAAAA TCTTCGAGGC CGAACCCGGT 
GTTAAACCTC CTGAGCCGGG CGAGAAGTTC TTCGGCAACT TCCCATACCC CTACATGAAT 
GGCCTGCTCC ATTTGGGCCA CGCCTTCACT CTTTCTAAGC TTGAGTTTGG AGCCGCCTAC 
CACCGCCTAC GTGGCTGTAA CGTTCTATTG CCTTTCGCTT TCCACTGCAC GGGAATGCCT 
ATCAAAGCAT CGGCCGACAA ACTTAAAAGA GAAATCGAAC AGCATGGAAA CCCTCCTGTT 
TTCCCCTCAT TAGAGGAGTT GACTATTGAA GTTGAAGAGA GTGATAATGT GTCTGCGGCT 
ACGCCCGATA AGTTTAAGAG CAAGAGGTCT AAAGCTGCTG CTAAGTCTAG CGGGGATAAG 
TCGCAGTGGG AAATTATGAG AAGTTTCGGC CTTTCCGACG AAGAGATCGC TAAGTTTCAA 
GACCCGTATC ATTGGCTTAA ACACTTCCCC CCGCTGGCTA AAGAGGATCT CAAGGCCTTT 
GGGCTAGGCT GCGATTGGAG GCGCTCTTTT ATAACGACTG ACATGAACCC GTTCTACGAT 
TCCTTTGTTA GGTGGCAGAT GAATAAGTTA AAGAACATGG GTAAGATCGT AAAGGATATG 
AGATACACTA TTTATTCACC GTTGGATGGC CAACCTTGCG CTGATCACGA TAGAGCCTCT 
GGCGAAGGAG TTCAACCTCA AGAATATGTA TTGATTAAGA TGGAGGTGAT CCCTCCTTTT 
CCAGCCAAGT TGAAGGCTTT GGAGGGTAGA AAAGTCTTCT TGGCGGCAGC TACGTTGAGG 
CCCGAGACAA TGTATGGGCA GACTAACTGT TGGGTATTGC CGGATGGGAA TTACGGAGCT 
TTCGAGATCA ATGATACCGA CGTTTTCATC CTCACTGAGA GGGCTGCGTT GAACTTAGCT 
TATCAGAAGC TGTCGAAAGT CCCTGAGAAG CTGAGCTGTT TGCTTAAGCT CTCTGGTCAT 
GACCTTATTG GCTTGCCGTT AAGTTCTCCT CTTGCTTTTA ATGAAGTGAT ATACTCACTT 
CCTATGCTAA CTATATTAAC TGATAAGGGT ACGGGCATTG TTACGAGTGT TCCGAGTGAC 
TCGCCAGATG ACTACATGGC TTTAAAGGAT TTAGTATCTA AACCGGCCTT ACGAGAAAAG 
TATGGCGTTA AAGATGAGTG GGTTCTGCCT TATGAGGTCG TGCCCATAAT AAATATTCCG 
GAGTTCGGAG ATAAATCGGC GGAAAAGGTG TGTATCGATC TTAAGATCAA GAGCCAGAAT 
GATAAGGAGA AACTCGCAGA GGCGAAGAGA CTGACTTATT TGAAAGGCTT TACGGATGGT 
AGTATGTTAG TAGGAGAATT CCAAGGGAGA AAGGTTCAAG AAGCGAAGCC GCTTATAAAG 
AACAAATTGT TGGAAGCTGG AATGGCCGTG CTGTACAGTG AGCCTGAAAA GAAAGTTATG 
TCGAGATCCG GTGATGAGTG TGTCGTGGCG CTTACGGATC AATGGTACAT AACTTATGGG 
GAGGTGGAGT GGAAGGAGAA GGCAATGGAG TGCCTAGAGA AAATGAATAC TTTTTCTACC 
GAGACCCGAA ATGGCTTCGA GCATACATTG AGCTGGCTAA ATCAGTGGGC TTGCTCACGG 
TCTTTCGGGC TCGGGACTCG AATTCCTTGG GACGAGCAGT TTCTCGTCGA ATCGCTTTCT 
GATTCGACCC TTTACATGGC TTATTACACT ATTGCGCATG TCTTGCAAAA TGGCGATATG 
TATGGATCAA ATGCTTCTTC AGTTAGACCA GAGCAGATGA CAGATGAGGT ATGGGATTAT 
ATCTTCTGCG ACGGCCCAGT TCCGAAATCT GAGATTTCTC CTGCTCTTCT AAGTAAAATG 
AAGAAAGAAT TCGAGTATTG GTATCCGTTT GATCTTAGGG TTTCAGGGAA AGATCTCATT 
CAGAACCATC TAACATTTTG CATATATAAC CATACAGCGC TTCTCCCGAA GCACTTATGG 
CCCCGAGGGT TCCGTTGCAA CGGGCATCTT ATGCTTAACT CCGAGAAAAT GTCCAAGTCT 
ACCGGAAACT TCCGCACACT TCGCCAGGCC ATAGAGGAAT TCTCCTCTGA CGCCACTCGG 
TTCTCTCTCG CAGATGCTGG CGACGGGATG GACGATGCGA ACTTCGTCTT CGAGACAGCA 
AATGCCGCGA TTTTGAGGCT GACTAAAGAA ATTGCCTGGA TGGAAGAGGT GCTTTCTGTC 
GAGTCTTCTT TGCGAGCCGG CCCCCCATCG TCTTATGCTG ACCGGGTTTT CGCCAATGAG 
ATGAATTTCG CGGTGGTGGA AACCGAGAAA AGTTACAACA ACTTCATGTT TAGAGAAGCT 
TTGAAAACCG GGTTTTACGA CCTTCAAGCT GCGAGAGATG AGTACAGATT TTCGTGCGGG 
GCGGGAGGTA TGAATAAGGA ATTGTTGTTC CGGTTTATGG ATGTTCAGAC CAGGCTTATC 
ACCCCGATTT GTCCACATTA CGCGGAACAT GTGTGGAAAA ATATATTGAA AAATGACGGG 
TTTATAGTAA GAGCCGGTTG GCCATTTGCA GAACCCCCGA ATCAAACGCT ACGAATCGCC 
AACAAGTATT TACAGGACTC TATTGTTTTG ATGAGAAAGC TGCTTCAGAA GCAGGAATCG 
GGTCCGAAAA AGGCTAAGAA GGGTGCTTCT GCTGCTCCTG CTGAAGAGAA CAAACTGACC 
GTCGGGCTTA TTTTCGTGAA CGAGCGATAC GATGGTTGGA AAGAAGAATG CTTGAGGATA 
CTACGAAGCA AATTCGATGA CACAACGGGT TCTTTTGCTC CCGACAAAGA GATTCTCGAG 
GCGCTAAAAT CAAGTTCTAT TGGAAATGAT GCTAATTTTA AGCAGATACA GAAGCTCTGC 
ATGCCTTTCA TAAAGTTTAA GAAGGACGAG ACGAAGGAAG TGGGTGTTCG CGCTTTAGAT 
TTGAAGCTAC CGTTTGGCGA AATCGAGGTT CTCAAGGAGA ATGTCGACGT GATAAAGAGG 
CAGTTGGGTC TCGAGCACGT GGAAGTTTTA TGCGCAACGG ACGATTCCGA TCGCAGTAAA 
TCCGGACCGC ACATCTCGTT GCTGACGCAG AATCCGCTGT CTCCTGGTAA TCCGGTTGCC 
ATTTTCCTCA GCAAAGCAGA ATTTTCGGCA ACCGGATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020092205.1
CDS	162..3443
Translation	MSTNTEGGGRSYARRDLLLKIQSEIQNYWEENKIFEAEPGVKPPEPGEKFFGNFPYPYMNGLLHLGHAFTLSKLEFGAAYHRLRGCNVLLPFAFHCTGMPIKASADKLKREIEQHGNPPVFPSLEELTIEVEESDNVSAATPDKFKSKRSKAAAKSSGDKSQWEIMRSFGLSDEEIAKFQDPYHWLKHFPPLAKEDLKAFGLGCDWRRSFITTDMNPFYDSFVRWQMNKLKNMGKIVKDMRYTIYSPLDGQPCADHDRASGEGVQPQEYVLIKMEVIPPFPAKLKALEGRKVFLAAATLRPETMYGQTNCWVLPDGNYGAFEINDTDVFILTERAALNLAYQKLSKVPEKLSCLLKLSGHDLIGLPLSSPLAFNEVIYSLPMLTILTDKGTGIVTSVPSDSPDDYMALKDLVSKPALREKYGVKDEWVLPYEVVPIINIPEFGDKSAEKVCIDLKIKSQNDKEKLAEAKRLTYLKGFTDGSMLVGEFQGRKVQEAKPLIKNKLLEAGMAVLYSEPEKKVMSRSGDECVVALTDQWYITYGEVEWKEKAMECLEKMNTFSTETRNGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTLYMAYYTIAHVLQNGDMYGSNASSVRPEQMTDEVWDYIFCDGPVPKSEISPALLSKMKKEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTALLPKHLWPRGFRCNGHLMLNSEKMSKSTGNFRTLRQAIEEFSSDATRFSLADAGDGMDDANFVFETANAAILRLTKEIAWMEEVLSVESSLRAGPPSSYADRVFANEMNFAVVETEKSYNNFMFREALKTGFYDLQAARDEYRFSCGAGGMNKELLFRFMDVQTRLITPICPHYAEHVWKNILKNDGFIVRAGWPFAEPPNQTLRIANKYLQDSIVLMRKLLQKQESGPKKAKKGASAAPAEENKLTVGLIFVNERYDGWKEECLRILRSKFDDTTGSFAPDKEILEALKSSSIGNDANFKQIQKLCMPFIKFKKDETKEVGVRALDLKLPFGEIEVLKENVDVIKRQLGLEHVEVLCATDDSDRSKSGPHISLLTQNPLSPGNPVAIFLSKAEFSATGY

Target ORF information:

RefSeq Version	XM_020236616.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus leucine--tRNA ligase, cytoplasmic-like (LOC109712834), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236616.1

ORF Insert Sequence:

ATGTCGACGA ATACTGAGGG AGGAGGAAGG AGCTATGCTC GGCGAGACCT ACTTCTCAAG 
ATACAATCAG AAATCCAAAA TTACTGGGAA GAAAACAAAA TCTTCGAGGC CGAACCCGGT 
GTTAAACCTC CTGAGCCGGG CGAGAAGTTC TTCGGCAACT TCCCATACCC CTACATGAAT 
GGCCTGCTCC ATTTGGGCCA CGCCTTCACT CTTTCTAAGC TTGAGTTTGG AGCCGCCTAC 
CACCGCCTAC GTGGCTGTAA CGTTCTATTG CCTTTCGCTT TCCACTGCAC GGGAATGCCT 
ATCAAAGCAT CGGCCGACAA ACTTAAAAGA GAAATCGAAC AGCATGGAAA CCCTCCTGTT 
TTCCCCTCAT TAGAGGAGTT GACTATTGAA GTTGAAGAGA GTGATAATGT GTCTGCGGCT 
ACGCCCGATA AGTTTAAGAG CAAGAGGTCT AAAGCTGCTG CTAAGTCTAG CGGGGATAAG 
TCGCAGTGGG AAATTATGAG AAGTTTCGGC CTTTCCGACG AAGAGATCGC TAAGTTTCAA 
GACCCGTATC ATTGGCTTAA ACACTTCCCC CCGCTGGCTA AAGAGGATCT CAAGGCCTTT 
GGGCTAGGCT GCGATTGGAG GCGCTCTTTT ATAACGACTG ACATGAACCC GTTCTACGAT 
TCCTTTGTTA GGTGGCAGAT GAATAAGTTA AAGAACATGG GTAAGATCGT AAAGGATATG 
AGATACACTA TTTATTCACC GTTGGATGGC CAACCTTGCG CTGATCACGA TAGAGCCTCT 
GGCGAAGGAG TTCAACCTCA AGAATATGTA TTGATTAAGA TGGAGGTGAT CCCTCCTTTT 
CCAGCCAAGT TGAAGGCTTT GGAGGGTAGA AAAGTCTTCT TGGCGGCAGC TACGTTGAGG 
CCCGAGACAA TGTATGGGCA GACTAACTGT TGGGTATTGC CGGATGGGAA TTACGGAGCT 
TTCGAGATCA ATGATACCGA CGTTTTCATC CTCACTGAGA GGGCTGCGTT GAACTTAGCT 
TATCAGAAGC TGTCGAAAGT CCCTGAGAAG CTGAGCTGTT TGCTTAAGCT CTCTGGTCAT 
GACCTTATTG GCTTGCCGTT AAGTTCTCCT CTTGCTTTTA ATGAAGTGAT ATACTCACTT 
CCTATGCTAA CTATATTAAC TGATAAGGGT ACGGGCATTG TTACGAGTGT TCCGAGTGAC 
TCGCCAGATG ACTACATGGC TTTAAAGGAT TTAGTATCTA AACCGGCCTT ACGAGAAAAG 
TATGGCGTTA AAGATGAGTG GGTTCTGCCT TATGAGGTCG TGCCCATAAT AAATATTCCG 
GAGTTCGGAG ATAAATCGGC GGAAAAGGTG TGTATCGATC TTAAGATCAA GAGCCAGAAT 
GATAAGGAGA AACTCGCAGA GGCGAAGAGA CTGACTTATT TGAAAGGCTT TACGGATGGT 
AGTATGTTAG TAGGAGAATT CCAAGGGAGA AAGGTTCAAG AAGCGAAGCC GCTTATAAAG 
AACAAATTGT TGGAAGCTGG AATGGCCGTG CTGTACAGTG AGCCTGAAAA GAAAGTTATG 
TCGAGATCCG GTGATGAGTG TGTCGTGGCG CTTACGGATC AATGGTACAT AACTTATGGG 
GAGGTGGAGT GGAAGGAGAA GGCAATGGAG TGCCTAGAGA AAATGAATAC TTTTTCTACC 
GAGACCCGAA ATGGCTTCGA GCATACATTG AGCTGGCTAA ATCAGTGGGC TTGCTCACGG 
TCTTTCGGGC TCGGGACTCG AATTCCTTGG GACGAGCAGT TTCTCGTCGA ATCGCTTTCT 
GATTCGACCC TTTACATGGC TTATTACACT ATTGCGCATG TCTTGCAAAA TGGCGATATG 
TATGGATCAA ATGCTTCTTC AGTTAGACCA GAGCAGATGA CAGATGAGGT ATGGGATTAT 
ATCTTCTGCG ACGGCCCAGT TCCGAAATCT GAGATTTCTC CTGCTCTTCT AAGTAAAATG 
AAGAAAGAAT TCGAGTATTG GTATCCGTTT GATCTTAGGG TTTCAGGGAA AGATCTCATT 
CAGAACCATC TAACATTTTG CATATATAAC CATACAGCGC TTCTCCCGAA GCACTTATGG 
CCCCGAGGGT TCCGTTGCAA CGGGCATCTT ATGCTTAACT CCGAGAAAAT GTCCAAGTCT 
ACCGGAAACT TCCGCACACT TCGCCAGGCC ATAGAGGAAT TCTCCTCTGA CGCCACTCGG 
TTCTCTCTCG CAGATGCTGG CGACGGGATG GACGATGCGA ACTTCGTCTT CGAGACAGCA 
AATGCCGCGA TTTTGAGGCT GACTAAAGAA ATTGCCTGGA TGGAAGAGGT GCTTTCTGTC 
GAGTCTTCTT TGCGAGCCGG CCCCCCATCG TCTTATGCTG ACCGGGTTTT CGCCAATGAG 
ATGAATTTCG CGGTGGTGGA AACCGAGAAA AGTTACAACA ACTTCATGTT TAGAGAAGCT 
TTGAAAACCG GGTTTTACGA CCTTCAAGCT GCGAGAGATG AGTACAGATT TTCGTGCGGG 
GCGGGAGGTA TGAATAAGGA ATTGTTGTTC CGGTTTATGG ATGTTCAGAC CAGGCTTATC 
ACCCCGATTT GTCCACATTA CGCGGAACAT GTGTGGAAAA ATATATTGAA AAATGACGGG 
TTTATAGTAA GAGCCGGTTG GCCATTTGCA GAACCCCCGA ATCAAACGCT ACGAATCGCC 
AACAAGTATT TACAGGACTC TATTGTTTTG ATGAGAAAGC TGCTTCAGAA GCAGGAATCG 
GGTCCGAAAA AGGCTAAGAA GGGTGCTTCT GCTGCTCCTG CTGAAGAGAA CAAACTGACC 
GTCGGGCTTA TTTTCGTGAA CGAGCGATAC GATGGTTGGA AAGAAGAATG CTTGAGGATA 
CTACGAAGCA AATTCGATGA CACAACGGGT TCTTTTGCTC CCGACAAAGA GATTCTCGAG 
GCGCTAAAAT CAAGTTCTAT TGGAAATGAT GCTAATTTTA AGCAGATACA GAAGCTCTGC 
ATGCCTTTCA TAAAGTTTAA GAAGGACGAG ACGAAGGAAG TGGGTGTTCG CGCTTTAGAT 
TTGAAGCTAC CGTTTGGCGA AATCGAGGTT CTCAAGGAGA ATGTCGACGT GATAAAGAGG 
CAGTTGGGTC TCGAGCACGT GGAAGTTTTA TGCGCAACGG ACGATTCCGA TCGCAGTAAA 
TCCGGACCGC ACATCTCGTT GCTGACGCAG AATCCGCTGT CTCCTGGTAA TCCGGTTGCC 
ATTTTCCTCA GCAAAGCAGA ATTTTCGGCA ACCGGATACT AG