pycard cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pycard
Entrez Gene ID	111578016
Full Name	PYD and CARD domain containing
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023284587.1	XP_023140355.1	apoptosis-associated speck-like protein containing a CARD

Pathways from BioSystems
Gene Ontology

The following pycard gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pycard cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm89160	XM_023284587.1 Latest version!	Amphiprion ocellaris PYD and CARD domain containing (pycard), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pycard ( XM_023284587.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023140355.1 Amphiprion ocellaris PYD and CARD domain containing (pycard), mRNA.

Price & Availability↑

CloneID	OAm89160
Clone ID Related Accession (Same CDS sequence)	XM_023284587.1
Accession Version	XM_023284587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	apoptosis-associated speck-like protein containing a CARD
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTA AACCCGTGAG ATCTGCCATG ATGGGCGCTC TGCAGAACCT GACCGATGCG 
GACCTGAAGA GGTTCATCCG TGAACTGTTG GCCCGCAAGG ACAAGCCCCG CGTCCCTCGC 
AATAAGGTGG AGGGCAAAGA CTACATGGAC GTAGCAGACG TGGTGGTTTC CACTTTCACG 
GAGAAACACG CCGTGACCGT GGCCGCGGAG ATCCTGAACA GCATCGGCTG CTCCGACGAG 
GCGGAGACAC TCGTTGAGGA GACCAGTGGA CAAACCTCAC AGTCTGCTTC CACTGATGTT 
GAGGAGACCA GTGGACAAAC CTCACAGTCT GCTTCCACTG ATGACATGCA CTTTGTGGAT 
AAACACAGAG GAGCACTGAT CCAGAGAGTG AGCAACATTG ATTCCATTTT GGATGATCTC 
CTTGAGAAAA AAGTTATCAA CCAGGAAGCT TATACCAAAA TCCGATTGAT CGCTACCCCT 
CAGGAGAAGA TGAGGGCCCT GTACGAAGGT CCTCTAAAAG CTGCAAAAGC CTGCAAGGAT 
ATCTTCTACC AAATCCTTCA GAAGCATGAA GAATATCTCA TCAAGGAGCT CGAAGGAAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023140355.1
CDS	31..636
Translation	MAGKPVRSAMMGALQNLTDADLKRFIRELLARKDKPRVPRNKVEGKDYMDVADVVVSTFTEKHAVTVAAEILNSIGCSDEAETLVEETSGQTSQSASTDVEETSGQTSQSASTDDMHFVDKHRGALIQRVSNIDSILDDLLEKKVINQEAYTKIRLIATPQEKMRALYEGPLKAAKACKDIFYQILQKHEEYLIKELEGKK

Target ORF information:

RefSeq Version	XM_023284587.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris PYD and CARD domain containing (pycard), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023284587.1

ORF Insert Sequence:

ATGGCCGGTA AACCCGTGAG ATCTGCCATG ATGGGCGCTC TGCAGAACCT GACCGATGCG 
GACCTGAAGA GGTTCATCCG TGAACTGTTG GCCCGCAAGG ACAAGCCCCG CGTCCCTCGC 
AATAAGGTGG AGGGCAAAGA CTACATGGAC GTAGCAGACG TGGTGGTTTC CACTTTCACG 
GAGAAACACG CCGTGACCGT GGCCGCGGAG ATCCTGAACA GCATCGGCTG CTCCGACGAG 
GCGGAGACAC TCGTTGAGGA GACCAGTGGA CAAACCTCAC AGTCTGCTTC CACTGATGTT 
GAGGAGACCA GTGGACAAAC CTCACAGTCT GCTTCCACTG ATGACATGCA CTTTGTGGAT 
AAACACAGAG GAGCACTGAT CCAGAGAGTG AGCAACATTG ATTCCATTTT GGATGATCTC 
CTTGAGAAAA AAGTTATCAA CCAGGAAGCT TATACCAAAA TCCGATTGAT CGCTACCCCT 
CAGGAGAAGA TGAGGGCCCT GTACGAAGGT CCTCTAAAAG CTGCAAAAGC CTGCAAGGAT 
ATCTTCTACC AAATCCTTCA GAAGCATGAA GAATATCTCA TCAAGGAGCT CGAAGGAAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.