nedd4 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nedd4
Entrez Gene ID	111577781
Full Name	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023284217.1	XP_023139985.1	E3 ubiquitin-protein ligase NEDD4 isoform X1
XM_023284218.1	XP_023139986.1	E3 ubiquitin-protein ligase NEDD4 isoform X2

Pathways from BioSystems
Gene Ontology

The following nedd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nedd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm88858	XM_023284218.1 Latest version!	Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm88857	XM_023284217.1 Latest version!	Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nedd4 ( XM_023284218.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023139986.1 Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm88858
Clone ID Related Accession (Same CDS sequence)	XM_023284218.1
Accession Version	XM_023284218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCTT TCTCCCCACA GATCCAGGGA CTTCAGTCAG ATGAGGATGG TACCAGAATC 
CTAAAAGTGA AAGTCATTGG TGGAATCGGA CTAGCCAAAA AAGATATTCT TGGAGCCAGT 
GATCCATACA CAAGGATGTC CCTGTATGAC CCTGTCAATG GAGAAATCGC AAGTCTTCAG 
ACTAAAACTA TTAAAAAGAC ACTCGACCCA AAGTGGAATG AAGAATTCTT TTTCAGAGTT 
GACCCCACTA AGCATCGCCT GCTGTTGGAG GTGTTTGATG AGAACCGCCT GACACGTGAT 
GACTTTCTCG GACAGGTGGA TGTCCCCTTA AATCAGATAC CGACAGAAAA TCCTAATTCA 
GAAAGGCCAT ACACATTCAG GGATTTTCTG CTTCACCCAC GAAGCCACAA GTCCAGAGTC 
AAAGGTCACC TGCGTCTCAA AATGACTTTC CTGCCAAAAA ATTCTGGTTC AGAGGAGGAA 
ACGACAGATC AGAGTGAGGA CACAGATCCT GGAGGCTGGG AGCTCCTGGA GAATCAGGAC 
ATGTCAGGTC CCAGAGAAAG CCAGCTGCTG CCTCCTCTGC CCCCTGGCTG GGAGGAGCGG 
CAGGACAACC TGGGAAGAAT TTACTTTGTC AATCATGAAA CCAGAACCAC ACAGTGGCTA 
CGACCCTCAG TACAGGATGC TAATTTGGAA ACTCGAAGGA TACAGAACAA TGATACAGAT 
GTGGAGCATG CTTTCATTAC ACGCAGGCAA ATCTCAGACC ATGATGAGAG CAACACACAG 
GAGTCTCCAG AGAGCTGGGA GATCATAACG GAGGATGAAT CCACTTTGTA CCACAGCAAC 
AACCAGATCA CATCACCTCC ACCTCAGGCG CCTGCAGATT TCCACCCGTT CATTGACGAG 
CTGAGAAATG TGCACATTAC TGGGGCCACA GCTGGCGAGC AGCGCACCTC CCAGACGAAT 
CATGCTAGTA ATTCAATCCA TTCCAGTCGC AGAGGAAGTG CCCAGGCTTC AGGCGGAGAG 
GAACATCCTG TTAATCCTGT GCTGCTTCCA ACCACAGCTG GGTTGCCTCC AGGCTGGGAG 
GAGAAACGGG ACAGTAAAGG AAGACGCTAC TACATCAACC ACAACAGCCG AACCACAACG 
TGGACACGAC CACTCATTCA GAGAACCTCA GATGCTACAG CACAGAGCAG TGCAAGTGTA 
CGCCGAAGCC CAACACCGCC CCAACCGCCT GCACCAACCG CACCACCCAC ACCGCCTGAG 
GAGTCTCCTC AGAGCACTCC AAGCCCCGAG GCGCCGTATG AATCTGGCTT ATTGCCGTCC 
GGTTGGGAGG TTCGCAGTGC TCCCAATGGA AGACCCTTTT TCATCGACCA CATCACGAAG 
ACGACTACCT GGGAAGATCC CAGGCTAAAG ATCCCTGTGC ACATGAGGAG GAGACCCTCA 
CTCGACCCTT CTGATCTCGG CCCTCTGCCG CCTGGTTGGG AAGAAAGGGT CCACTCTGAT 
GGGAGGATAT TCTACATTGA TCATAACACC AAGACCACAC AATGGGATGA TCCCAGATTA 
CAGAACTCAG CTATAACTGG ACCAGCAGTG CCTTACTCTA GAGACTACAA ACAGAAGTAT 
GACTACTTCA GAAAGAAGCT GAAGAAACCG GCCGACATCC CGAATCGTTT TGAGATGAAG 
ATGAGACGAA ATGCAGTGCT GGAGGACTCA TACCGACGCA TCCTCTCAGT GAAGAAAGCG 
GACCTGCTGA AAGCCCGACT GTGGGTGGAG TTTGAAGGAG AAAAAGGACT GGACTACGGT 
GGCCTGGCCA GGGAGTGGTT TTTCCTCATG TCCAAGGAGA TGTTCAACCC GTACTATGGA 
CTGTTTGAGT ATTCTGCCAC GGACAACTAC ACCCTGCAGA TTAACCCTAA CTCAGGTTTA 
TGCAATGAGG ACCACCTGTC CTACTTCAAG TTCATCGGCC GAGTGGCAGG AATGGCCGTG 
TATCACGGCA AACTCCTTGA TGCTTTCTTC ATTCGGCCGT TCTACAAGAT GATGCTGCAG 
AAGCCGATCA CGCTCCAGGA CATGGAGTCT GTTGACAGTG AATATTTCAA CTCCTTGATG 
TGGATTTTGG AGAATGATCC AACAGACTTG GATCTGAGGT TCACCATCGA TGAGGAGCTG 
TTTGGACAGA CTCACCAGCA TGAGCTGAAG CCAGGAGGCA CTGAGATTGT CGTCACAAAC 
GAAAACAAGA AGGAGTACAT CCACCATGTG ATGCAGTGGA GGTTTGTAAA CAGAATACAG 
AAGCAGATGA CCGCTTTCAA GGAGGGATTC TTTGAGTTGA TACCCCAAGA TCTGATCAAG 
ATCTTTGACG AGAATGAGCT TGAGCTGCTC ATGTGTGGTC TCGGAGATGT GGATGTGAAT 
GACTGGAAGG CGAACACCAA GTACAAGAAC AGCTACTGCT CCAACCACGT AGTTATCCAG 
TGGTTTTGGA AGACAGTGCT GCTGATGGAT GCAGAGAAGC GAATCCGGCT CTTACAGTTT 
GTGACAGGAA CATCCAGAGT CCCGATGAAT GGCTTTGCTG AACTCTATGG GTCTAACGGG 
CCACAGCTGT TCACCATCGA GCAGTGGGGA ACACGGGATA AACTTCCCCG GGCCCACACA 
TGCTTCAACC GGCTGGACCT TCCTCCTTAC GAGTCCTTTG AAGAACTGAG GGAGAAACTT 
CACATCGCTA TTGAGAACGC ACAAGGCTTC GACGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023139986.1
CDS	196..2940
Translation	MAAFSPQIQGLQSDEDGTRILKVKVIGGIGLAKKDILGASDPYTRMSLYDPVNGEIASLQTKTIKKTLDPKWNEEFFFRVDPTKHRLLLEVFDENRLTRDDFLGQVDVPLNQIPTENPNSERPYTFRDFLLHPRSHKSRVKGHLRLKMTFLPKNSGSEEETTDQSEDTDPGGWELLENQDMSGPRESQLLPPLPPGWEERQDNLGRIYFVNHETRTTQWLRPSVQDANLETRRIQNNDTDVEHAFITRRQISDHDESNTQESPESWEIITEDESTLYHSNNQITSPPPQAPADFHPFIDELRNVHITGATAGEQRTSQTNHASNSIHSSRRGSAQASGGEEHPVNPVLLPTTAGLPPGWEEKRDSKGRRYYINHNSRTTTWTRPLIQRTSDATAQSSASVRRSPTPPQPPAPTAPPTPPEESPQSTPSPEAPYESGLLPSGWEVRSAPNGRPFFIDHITKTTTWEDPRLKIPVHMRRRPSLDPSDLGPLPPGWEERVHSDGRIFYIDHNTKTTQWDDPRLQNSAITGPAVPYSRDYKQKYDYFRKKLKKPADIPNRFEMKMRRNAVLEDSYRRILSVKKADLLKARLWVEFEGEKGLDYGGLAREWFFLMSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLQDMESVDSEYFNSLMWILENDPTDLDLRFTIDEELFGQTHQHELKPGGTEIVVTNENKKEYIHHVMQWRFVNRIQKQMTAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWKANTKYKNSYCSNHVVIQWFWKTVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTRDKLPRAHTCFNRLDLPPYESFEELREKLHIAIENAQGFDGVD

Target ORF information:

RefSeq Version	XM_023284218.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023284218.1

ORF Insert Sequence:

ATGGCCGCTT TCTCCCCACA GATCCAGGGA CTTCAGTCAG ATGAGGATGG TACCAGAATC 
CTAAAAGTGA AAGTCATTGG TGGAATCGGA CTAGCCAAAA AAGATATTCT TGGAGCCAGT 
GATCCATACA CAAGGATGTC CCTGTATGAC CCTGTCAATG GAGAAATCGC AAGTCTTCAG 
ACTAAAACTA TTAAAAAGAC ACTCGACCCA AAGTGGAATG AAGAATTCTT TTTCAGAGTT 
GACCCCACTA AGCATCGCCT GCTGTTGGAG GTGTTTGATG AGAACCGCCT GACACGTGAT 
GACTTTCTCG GACAGGTGGA TGTCCCCTTA AATCAGATAC CGACAGAAAA TCCTAATTCA 
GAAAGGCCAT ACACATTCAG GGATTTTCTG CTTCACCCAC GAAGCCACAA GTCCAGAGTC 
AAAGGTCACC TGCGTCTCAA AATGACTTTC CTGCCAAAAA ATTCTGGTTC AGAGGAGGAA 
ACGACAGATC AGAGTGAGGA CACAGATCCT GGAGGCTGGG AGCTCCTGGA GAATCAGGAC 
ATGTCAGGTC CCAGAGAAAG CCAGCTGCTG CCTCCTCTGC CCCCTGGCTG GGAGGAGCGG 
CAGGACAACC TGGGAAGAAT TTACTTTGTC AATCATGAAA CCAGAACCAC ACAGTGGCTA 
CGACCCTCAG TACAGGATGC TAATTTGGAA ACTCGAAGGA TACAGAACAA TGATACAGAT 
GTGGAGCATG CTTTCATTAC ACGCAGGCAA ATCTCAGACC ATGATGAGAG CAACACACAG 
GAGTCTCCAG AGAGCTGGGA GATCATAACG GAGGATGAAT CCACTTTGTA CCACAGCAAC 
AACCAGATCA CATCACCTCC ACCTCAGGCG CCTGCAGATT TCCACCCGTT CATTGACGAG 
CTGAGAAATG TGCACATTAC TGGGGCCACA GCTGGCGAGC AGCGCACCTC CCAGACGAAT 
CATGCTAGTA ATTCAATCCA TTCCAGTCGC AGAGGAAGTG CCCAGGCTTC AGGCGGAGAG 
GAACATCCTG TTAATCCTGT GCTGCTTCCA ACCACAGCTG GGTTGCCTCC AGGCTGGGAG 
GAGAAACGGG ACAGTAAAGG AAGACGCTAC TACATCAACC ACAACAGCCG AACCACAACG 
TGGACACGAC CACTCATTCA GAGAACCTCA GATGCTACAG CACAGAGCAG TGCAAGTGTA 
CGCCGAAGCC CAACACCGCC CCAACCGCCT GCACCAACCG CACCACCCAC ACCGCCTGAG 
GAGTCTCCTC AGAGCACTCC AAGCCCCGAG GCGCCGTATG AATCTGGCTT ATTGCCGTCC 
GGTTGGGAGG TTCGCAGTGC TCCCAATGGA AGACCCTTTT TCATCGACCA CATCACGAAG 
ACGACTACCT GGGAAGATCC CAGGCTAAAG ATCCCTGTGC ACATGAGGAG GAGACCCTCA 
CTCGACCCTT CTGATCTCGG CCCTCTGCCG CCTGGTTGGG AAGAAAGGGT CCACTCTGAT 
GGGAGGATAT TCTACATTGA TCATAACACC AAGACCACAC AATGGGATGA TCCCAGATTA 
CAGAACTCAG CTATAACTGG ACCAGCAGTG CCTTACTCTA GAGACTACAA ACAGAAGTAT 
GACTACTTCA GAAAGAAGCT GAAGAAACCG GCCGACATCC CGAATCGTTT TGAGATGAAG 
ATGAGACGAA ATGCAGTGCT GGAGGACTCA TACCGACGCA TCCTCTCAGT GAAGAAAGCG 
GACCTGCTGA AAGCCCGACT GTGGGTGGAG TTTGAAGGAG AAAAAGGACT GGACTACGGT 
GGCCTGGCCA GGGAGTGGTT TTTCCTCATG TCCAAGGAGA TGTTCAACCC GTACTATGGA 
CTGTTTGAGT ATTCTGCCAC GGACAACTAC ACCCTGCAGA TTAACCCTAA CTCAGGTTTA 
TGCAATGAGG ACCACCTGTC CTACTTCAAG TTCATCGGCC GAGTGGCAGG AATGGCCGTG 
TATCACGGCA AACTCCTTGA TGCTTTCTTC ATTCGGCCGT TCTACAAGAT GATGCTGCAG 
AAGCCGATCA CGCTCCAGGA CATGGAGTCT GTTGACAGTG AATATTTCAA CTCCTTGATG 
TGGATTTTGG AGAATGATCC AACAGACTTG GATCTGAGGT TCACCATCGA TGAGGAGCTG 
TTTGGACAGA CTCACCAGCA TGAGCTGAAG CCAGGAGGCA CTGAGATTGT CGTCACAAAC 
GAAAACAAGA AGGAGTACAT CCACCATGTG ATGCAGTGGA GGTTTGTAAA CAGAATACAG 
AAGCAGATGA CCGCTTTCAA GGAGGGATTC TTTGAGTTGA TACCCCAAGA TCTGATCAAG 
ATCTTTGACG AGAATGAGCT TGAGCTGCTC ATGTGTGGTC TCGGAGATGT GGATGTGAAT 
GACTGGAAGG CGAACACCAA GTACAAGAAC AGCTACTGCT CCAACCACGT AGTTATCCAG 
TGGTTTTGGA AGACAGTGCT GCTGATGGAT GCAGAGAAGC GAATCCGGCT CTTACAGTTT 
GTGACAGGAA CATCCAGAGT CCCGATGAAT GGCTTTGCTG AACTCTATGG GTCTAACGGG 
CCACAGCTGT TCACCATCGA GCAGTGGGGA ACACGGGATA AACTTCCCCG GGCCCACACA 
TGCTTCAACC GGCTGGACCT TCCTCCTTAC GAGTCCTTTG AAGAACTGAG GGAGAAACTT 
CACATCGCTA TTGAGAACGC ACAAGGCTTC GACGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nedd4 ( XM_023284217.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023139985.1 Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm88857
Clone ID Related Accession (Same CDS sequence)	XM_023284217.1
Accession Version	XM_023284217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTC GGCTCCGCTT GCATTTTGCG TCAAGGAGAA GCAACACGGA CCCTCTGTCA 
GAAAGCCTCA GCAGCCATGG TGAGGAGAGT GGAGTCAGTG TGTCAGCTCA TAGCCCCACA 
GAGAGTCTGG CGCACAGTTC TGTCCACTTG AAGGTGACTC CCCTGTCACC AGACTATGGT 
AGTAATCGCT ACTCTTCAGT ATTTATACCC AGAGTTAACA CTGGAGGATG TAATAAGAAG 
AGCATACTGC AGATCTCGCT GCAGCCCTGT GGAAAGCTCA GTGGAGAGCT GGATGGCAGT 
ATAGAGGATG GAGACCCGGT GGTTAGTGAA GGAGGAGCAG GTACTGGCTC TGACGGAGGC 
TCGTGTAGCA GCAGCATGGC CAGCGATGCC GGGTACTGTA GCAGCAACAG CATCTTTGAG 
CCAGAGGCTC CAGAAAAGCA CAGGACCACC CAGGAGAGGA GTGTACTCTC CTGCAAGTCC 
AAGGTCCCAC TGAGACGGTG CTCCTCCTTG GTTATATTTC CAAAGAGTCC CTGCAGCACC 
CCACCTGCTT CCCCAGTAAG TCCAGTGGCT TTGCCTGCTC TCCCACCGTT AAGGGGGTCT 
AATCAGTCAT CTCTTCAGAC ATCTGCCAGT GAATACTCAC AAGACGATGA GGAGGCGATT 
TGCAAAGGGT CCACCACCAC GGCTGTAAGT GGTCTCCGTC ACCCAAAAGG AAACTGTTCA 
ACTGAGTTCA GGGACATGAA ACACATGGTG CAATTTAATA TTCCTTTACA GGATGAACCA 
AAATGCAAAA TGGAGGACAG GAGTAAAGTG ATGGAACTGT CATCGGCTCT AGACAGATCA 
AATCGTCACT CTAGCTGCGT GTTGCTGCAC TTTGCCCACC AGAGACCAAT GATATCGGGG 
AAGGGAGCTA CAACCACGGC TACAGTCAAT GTGGAATACC CTGAGACTCA TTACCCAGCT 
ACACACCCTG AGGGTGAACA CAACGAACAC AAAAAACTGT ATCGTAGTAC CTCTGCGTGT 
TTGTTTTCCT CAACCAAGCC ATCAGAGAAA AACCATAAGG TCTGTTCAGC AGAGAAAGGC 
CAAGAAAGGC CTGAGGAAAA TCACTGTCAT CGTGCCATAC AAAGGAGCTT TTCCTTGGAG 
GTGCCCTACG CTAATACTGG AATTTCCTGT CATGTCTCGA ACCCAAAACT CAGCAGCCCT 
TGCTCCCCCC ATGTGCATAT TCATTTGTCC CCTGGCTGCC CAGCTAAGTT GCCTGGTTCT 
GTCACTGACG TTAACACAGG CCACAAAGGG AATAACGCAC CAAAGAGCCC AGGTCAGAAT 
ACCAAGACAC GTGATGACTT TCTCGGACAG GTGGATGTCC CCTTAAATCA GATACCGACA 
GAAAATCCTA ATTCAGAAAG GCCATACACA TTCAGGGATT TTCTGCTTCA CCCACGAAGC 
CACAAGTCCA GAGTCAAAGG TCACCTGCGT CTCAAAATGA CTTTCCTGCC AAAAAATTCT 
GGTTCAGAGG AGGAAACGAC AGATCAGAGT GAGGACACAG ATCCTGGAGG CTGGGAGCTC 
CTGGAGAATC AGGACATGTC AGGTCCCAGA GAAAGCCAGC TGCTGCCTCC TCTGCCCCCT 
GGCTGGGAGG AGCGGCAGGA CAACCTGGGA AGAATTTACT TTGTCAATCA TGAAACCAGA 
ACCACACAGT GGCTACGACC CTCAGTACAG GATGCTAATT TGGAAACTCG AAGGATACAG 
AACAATGATA CAGATGTGGA GCATGCTTTC ATTACACGCA GGCAAATCTC AGACCATGAT 
GAGAGCAACA CACAGGAGTC TCCAGAGAGC TGGGAGATCA TAACGGAGGA TGAATCCACT 
TTGTACCACA GCAACAACCA GATCACATCA CCTCCACCTC AGGCGCCTGC AGATTTCCAC 
CCGTTCATTG ACGAGCTGAG AAATGTGCAC ATTACTGGGG CCACAGCTGG CGAGCAGCGC 
ACCTCCCAGA CGAATCATGC TAGTAATTCA ATCCATTCCA GTCGCAGAGG AAGTGCCCAG 
GCTTCAGGCG GAGAGGAACA TCCTGTTAAT CCTGTGCTGC TTCCAACCAC AGCTGGGTTG 
CCTCCAGGCT GGGAGGAGAA ACGGGACAGT AAAGGAAGAC GCTACTACAT CAACCACAAC 
AGCCGAACCA CAACGTGGAC ACGACCACTC ATTCAGAGAA CCTCAGATGC TACAGCACAG 
AGCAGTGCAA GTGTACGCCG AAGCCCAACA CCGCCCCAAC CGCCTGCACC AACCGCACCA 
CCCACACCGC CTGAGGAGTC TCCTCAGAGC ACTCCAAGCC CCGAGGCGCC GTATGAATCT 
GGCTTATTGC CGTCCGGTTG GGAGGTTCGC AGTGCTCCCA ATGGAAGACC CTTTTTCATC 
GACCACATCA CGAAGACGAC TACCTGGGAA GATCCCAGGC TAAAGATCCC TGTGCACATG 
AGGAGGAGAC CCTCACTCGA CCCTTCTGAT CTCGGCCCTC TGCCGCCTGG TTGGGAAGAA 
AGGGTCCACT CTGATGGGAG GATATTCTAC ATTGATCATA ACACCAAGAC CACACAATGG 
GATGATCCCA GATTACAGAA CTCAGCTATA ACTGGACCAG CAGTGCCTTA CTCTAGAGAC 
TACAAACAGA AGTATGACTA CTTCAGAAAG AAGCTGAAGA AACCGGCCGA CATCCCGAAT 
CGTTTTGAGA TGAAGATGAG ACGAAATGCA GTGCTGGAGG ACTCATACCG ACGCATCCTC 
TCAGTGAAGA AAGCGGACCT GCTGAAAGCC CGACTGTGGG TGGAGTTTGA AGGAGAAAAA 
GGACTGGACT ACGGTGGCCT GGCCAGGGAG TGGTTTTTCC TCATGTCCAA GGAGATGTTC 
AACCCGTACT ATGGACTGTT TGAGTATTCT GCCACGGACA ACTACACCCT GCAGATTAAC 
CCTAACTCAG GTTTATGCAA TGAGGACCAC CTGTCCTACT TCAAGTTCAT CGGCCGAGTG 
GCAGGAATGG CCGTGTATCA CGGCAAACTC CTTGATGCTT TCTTCATTCG GCCGTTCTAC 
AAGATGATGC TGCAGAAGCC GATCACGCTC CAGGACATGG AGTCTGTTGA CAGTGAATAT 
TTCAACTCCT TGATGTGGAT TTTGGAGAAT GATCCAACAG ACTTGGATCT GAGGTTCACC 
ATCGATGAGG AGCTGTTTGG ACAGACTCAC CAGCATGAGC TGAAGCCAGG AGGCACTGAG 
ATTGTCGTCA CAAACGAAAA CAAGAAGGAG TACATCCACC ATGTGATGCA GTGGAGGTTT 
GTAAACAGAA TACAGAAGCA GATGACCGCT TTCAAGGAGG GATTCTTTGA GTTGATACCC 
CAAGATCTGA TCAAGATCTT TGACGAGAAT GAGCTTGAGC TGCTCATGTG TGGTCTCGGA 
GATGTGGATG TGAATGACTG GAAGGCGAAC ACCAAGTACA AGAACAGCTA CTGCTCCAAC 
CACGTAGTTA TCCAGTGGTT TTGGAAGACA GTGCTGCTGA TGGATGCAGA GAAGCGAATC 
CGGCTCTTAC AGTTTGTGAC AGGAACATCC AGAGTCCCGA TGAATGGCTT TGCTGAACTC 
TATGGGTCTA ACGGGCCACA GCTGTTCACC ATCGAGCAGT GGGGAACACG GGATAAACTT 
CCCCGGGCCC ACACATGCTT CAACCGGCTG GACCTTCCTC CTTACGAGTC CTTTGAAGAA 
CTGAGGGAGA AACTTCACAT CGCTATTGAG AACGCACAAG GCTTCGACGG GGTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023139985.1
CDS	1..3780
Translation	MARRLRLHFASRRSNTDPLSESLSSHGEESGVSVSAHSPTESLAHSSVHLKVTPLSPDYGSNRYSSVFIPRVNTGGCNKKSILQISLQPCGKLSGELDGSIEDGDPVVSEGGAGTGSDGGSCSSSMASDAGYCSSNSIFEPEAPEKHRTTQERSVLSCKSKVPLRRCSSLVIFPKSPCSTPPASPVSPVALPALPPLRGSNQSSLQTSASEYSQDDEEAICKGSTTTAVSGLRHPKGNCSTEFRDMKHMVQFNIPLQDEPKCKMEDRSKVMELSSALDRSNRHSSCVLLHFAHQRPMISGKGATTTATVNVEYPETHYPATHPEGEHNEHKKLYRSTSACLFSSTKPSEKNHKVCSAEKGQERPEENHCHRAIQRSFSLEVPYANTGISCHVSNPKLSSPCSPHVHIHLSPGCPAKLPGSVTDVNTGHKGNNAPKSPGQNTKTRDDFLGQVDVPLNQIPTENPNSERPYTFRDFLLHPRSHKSRVKGHLRLKMTFLPKNSGSEEETTDQSEDTDPGGWELLENQDMSGPRESQLLPPLPPGWEERQDNLGRIYFVNHETRTTQWLRPSVQDANLETRRIQNNDTDVEHAFITRRQISDHDESNTQESPESWEIITEDESTLYHSNNQITSPPPQAPADFHPFIDELRNVHITGATAGEQRTSQTNHASNSIHSSRRGSAQASGGEEHPVNPVLLPTTAGLPPGWEEKRDSKGRRYYINHNSRTTTWTRPLIQRTSDATAQSSASVRRSPTPPQPPAPTAPPTPPEESPQSTPSPEAPYESGLLPSGWEVRSAPNGRPFFIDHITKTTTWEDPRLKIPVHMRRRPSLDPSDLGPLPPGWEERVHSDGRIFYIDHNTKTTQWDDPRLQNSAITGPAVPYSRDYKQKYDYFRKKLKKPADIPNRFEMKMRRNAVLEDSYRRILSVKKADLLKARLWVEFEGEKGLDYGGLAREWFFLMSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLQDMESVDSEYFNSLMWILENDPTDLDLRFTIDEELFGQTHQHELKPGGTEIVVTNENKKEYIHHVMQWRFVNRIQKQMTAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWKANTKYKNSYCSNHVVIQWFWKTVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTRDKLPRAHTCFNRLDLPPYESFEELREKLHIAIENAQGFDGVD

Target ORF information:

RefSeq Version	XM_023284217.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023284217.1

ORF Insert Sequence:

ATGGCACGTC GGCTCCGCTT GCATTTTGCG TCAAGGAGAA GCAACACGGA CCCTCTGTCA 
GAAAGCCTCA GCAGCCATGG TGAGGAGAGT GGAGTCAGTG TGTCAGCTCA TAGCCCCACA 
GAGAGTCTGG CGCACAGTTC TGTCCACTTG AAGGTGACTC CCCTGTCACC AGACTATGGT 
AGTAATCGCT ACTCTTCAGT ATTTATACCC AGAGTTAACA CTGGAGGATG TAATAAGAAG 
AGCATACTGC AGATCTCGCT GCAGCCCTGT GGAAAGCTCA GTGGAGAGCT GGATGGCAGT 
ATAGAGGATG GAGACCCGGT GGTTAGTGAA GGAGGAGCAG GTACTGGCTC TGACGGAGGC 
TCGTGTAGCA GCAGCATGGC CAGCGATGCC GGGTACTGTA GCAGCAACAG CATCTTTGAG 
CCAGAGGCTC CAGAAAAGCA CAGGACCACC CAGGAGAGGA GTGTACTCTC CTGCAAGTCC 
AAGGTCCCAC TGAGACGGTG CTCCTCCTTG GTTATATTTC CAAAGAGTCC CTGCAGCACC 
CCACCTGCTT CCCCAGTAAG TCCAGTGGCT TTGCCTGCTC TCCCACCGTT AAGGGGGTCT 
AATCAGTCAT CTCTTCAGAC ATCTGCCAGT GAATACTCAC AAGACGATGA GGAGGCGATT 
TGCAAAGGGT CCACCACCAC GGCTGTAAGT GGTCTCCGTC ACCCAAAAGG AAACTGTTCA 
ACTGAGTTCA GGGACATGAA ACACATGGTG CAATTTAATA TTCCTTTACA GGATGAACCA 
AAATGCAAAA TGGAGGACAG GAGTAAAGTG ATGGAACTGT CATCGGCTCT AGACAGATCA 
AATCGTCACT CTAGCTGCGT GTTGCTGCAC TTTGCCCACC AGAGACCAAT GATATCGGGG 
AAGGGAGCTA CAACCACGGC TACAGTCAAT GTGGAATACC CTGAGACTCA TTACCCAGCT 
ACACACCCTG AGGGTGAACA CAACGAACAC AAAAAACTGT ATCGTAGTAC CTCTGCGTGT 
TTGTTTTCCT CAACCAAGCC ATCAGAGAAA AACCATAAGG TCTGTTCAGC AGAGAAAGGC 
CAAGAAAGGC CTGAGGAAAA TCACTGTCAT CGTGCCATAC AAAGGAGCTT TTCCTTGGAG 
GTGCCCTACG CTAATACTGG AATTTCCTGT CATGTCTCGA ACCCAAAACT CAGCAGCCCT 
TGCTCCCCCC ATGTGCATAT TCATTTGTCC CCTGGCTGCC CAGCTAAGTT GCCTGGTTCT 
GTCACTGACG TTAACACAGG CCACAAAGGG AATAACGCAC CAAAGAGCCC AGGTCAGAAT 
ACCAAGACAC GTGATGACTT TCTCGGACAG GTGGATGTCC CCTTAAATCA GATACCGACA 
GAAAATCCTA ATTCAGAAAG GCCATACACA TTCAGGGATT TTCTGCTTCA CCCACGAAGC 
CACAAGTCCA GAGTCAAAGG TCACCTGCGT CTCAAAATGA CTTTCCTGCC AAAAAATTCT 
GGTTCAGAGG AGGAAACGAC AGATCAGAGT GAGGACACAG ATCCTGGAGG CTGGGAGCTC 
CTGGAGAATC AGGACATGTC AGGTCCCAGA GAAAGCCAGC TGCTGCCTCC TCTGCCCCCT 
GGCTGGGAGG AGCGGCAGGA CAACCTGGGA AGAATTTACT TTGTCAATCA TGAAACCAGA 
ACCACACAGT GGCTACGACC CTCAGTACAG GATGCTAATT TGGAAACTCG AAGGATACAG 
AACAATGATA CAGATGTGGA GCATGCTTTC ATTACACGCA GGCAAATCTC AGACCATGAT 
GAGAGCAACA CACAGGAGTC TCCAGAGAGC TGGGAGATCA TAACGGAGGA TGAATCCACT 
TTGTACCACA GCAACAACCA GATCACATCA CCTCCACCTC AGGCGCCTGC AGATTTCCAC 
CCGTTCATTG ACGAGCTGAG AAATGTGCAC ATTACTGGGG CCACAGCTGG CGAGCAGCGC 
ACCTCCCAGA CGAATCATGC TAGTAATTCA ATCCATTCCA GTCGCAGAGG AAGTGCCCAG 
GCTTCAGGCG GAGAGGAACA TCCTGTTAAT CCTGTGCTGC TTCCAACCAC AGCTGGGTTG 
CCTCCAGGCT GGGAGGAGAA ACGGGACAGT AAAGGAAGAC GCTACTACAT CAACCACAAC 
AGCCGAACCA CAACGTGGAC ACGACCACTC ATTCAGAGAA CCTCAGATGC TACAGCACAG 
AGCAGTGCAA GTGTACGCCG AAGCCCAACA CCGCCCCAAC CGCCTGCACC AACCGCACCA 
CCCACACCGC CTGAGGAGTC TCCTCAGAGC ACTCCAAGCC CCGAGGCGCC GTATGAATCT 
GGCTTATTGC CGTCCGGTTG GGAGGTTCGC AGTGCTCCCA ATGGAAGACC CTTTTTCATC 
GACCACATCA CGAAGACGAC TACCTGGGAA GATCCCAGGC TAAAGATCCC TGTGCACATG 
AGGAGGAGAC CCTCACTCGA CCCTTCTGAT CTCGGCCCTC TGCCGCCTGG TTGGGAAGAA 
AGGGTCCACT CTGATGGGAG GATATTCTAC ATTGATCATA ACACCAAGAC CACACAATGG 
GATGATCCCA GATTACAGAA CTCAGCTATA ACTGGACCAG CAGTGCCTTA CTCTAGAGAC 
TACAAACAGA AGTATGACTA CTTCAGAAAG AAGCTGAAGA AACCGGCCGA CATCCCGAAT 
CGTTTTGAGA TGAAGATGAG ACGAAATGCA GTGCTGGAGG ACTCATACCG ACGCATCCTC 
TCAGTGAAGA AAGCGGACCT GCTGAAAGCC CGACTGTGGG TGGAGTTTGA AGGAGAAAAA 
GGACTGGACT ACGGTGGCCT GGCCAGGGAG TGGTTTTTCC TCATGTCCAA GGAGATGTTC 
AACCCGTACT ATGGACTGTT TGAGTATTCT GCCACGGACA ACTACACCCT GCAGATTAAC 
CCTAACTCAG GTTTATGCAA TGAGGACCAC CTGTCCTACT TCAAGTTCAT CGGCCGAGTG 
GCAGGAATGG CCGTGTATCA CGGCAAACTC CTTGATGCTT TCTTCATTCG GCCGTTCTAC 
AAGATGATGC TGCAGAAGCC GATCACGCTC CAGGACATGG AGTCTGTTGA CAGTGAATAT 
TTCAACTCCT TGATGTGGAT TTTGGAGAAT GATCCAACAG ACTTGGATCT GAGGTTCACC 
ATCGATGAGG AGCTGTTTGG ACAGACTCAC CAGCATGAGC TGAAGCCAGG AGGCACTGAG 
ATTGTCGTCA CAAACGAAAA CAAGAAGGAG TACATCCACC ATGTGATGCA GTGGAGGTTT 
GTAAACAGAA TACAGAAGCA GATGACCGCT TTCAAGGAGG GATTCTTTGA GTTGATACCC 
CAAGATCTGA TCAAGATCTT TGACGAGAAT GAGCTTGAGC TGCTCATGTG TGGTCTCGGA 
GATGTGGATG TGAATGACTG GAAGGCGAAC ACCAAGTACA AGAACAGCTA CTGCTCCAAC 
CACGTAGTTA TCCAGTGGTT TTGGAAGACA GTGCTGCTGA TGGATGCAGA GAAGCGAATC 
CGGCTCTTAC AGTTTGTGAC AGGAACATCC AGAGTCCCGA TGAATGGCTT TGCTGAACTC 
TATGGGTCTA ACGGGCCACA GCTGTTCACC ATCGAGCAGT GGGGAACACG GGATAAACTT 
CCCCGGGCCC ACACATGCTT CAACCGGCTG GACCTTCCTC CTTACGAGTC CTTTGAAGAA 
CTGAGGGAGA AACTTCACAT CGCTATTGAG AACGCACAAG GCTTCGACGG GGTGGATTAG