pelo cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pelo
Entrez Gene ID	111568687
Full Name	pelota mRNA surveillance and ribosome rescue factor
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023270453.1	XP_023126221.1	protein pelota homolog

Pathways from BioSystems
Gene Ontology

The following pelo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pelo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm71745	XM_023270453.1 Latest version!	Amphiprion ocellaris pelota mRNA surveillance and ribosome rescue factor (pelo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pelo ( XM_023270453.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023126221.1 Amphiprion ocellaris pelota mRNA surveillance and ribosome rescue factor (pelo), mRNA.

Price & Availability↑

CloneID	OAm71745
Clone ID Related Accession (Same CDS sequence)	XM_023270453.1
Accession Version	XM_023270453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	protein pelota homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019370148.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA TCCATAAAGA CATTGAAAAA GATAATGCCG GCCAGGTGAC TCTGGTGCCA 
GAGGAGGCGG AGGACATGTG GCACACCTAC AACCTGCTCC AAGTGGGGGA CAGCTTGAGA 
GCCTCAACTA TCAGAAAGGT GCAGACAGAG TCAACCACTG GAAGTGTGGG CAGCTCCAGA 
GTTCGGACCA CTCTAACAAT ATGTGTGGAG ACTATCGACT TTGACTCTCA TGCATGTCAG 
CTGAGAGTAA AAGGCACCAA CATAGTGGAG AACCAGTATG TCAAGATGGG GGCTTATCAC 
ACTATTGAGC TTGAGCTCAA TAGGAAGTTT ACTCTCGCTA AGAAGTGCTG GGACAGCGTT 
GTGCTGGATA GAATCGATCA AGCATGTGAT GCAGCCCAGA AGGCAGATGT GGCAGCTGTG 
GTGATGCAGG AGGGTCTGGC CAACCTGGTA CTGGTGACCC CGGCCATGAC TCTGCTTCGT 
GCAAAAGTGG AAGTCACTAT TCCTCGCAAG AGAAGGGGCA GCTGCACTCA GCACGAGAAG 
GCGCTGGAGA GGTTTTATGA GGCTGTGATG CAGGCGATAC TTCGCCACAT CAATTTTGAT 
GTGGTGAAGT GTATCCTGGT GGCGAGTCCA GGGTTTGTGA AGGACCAGTT TATCACCTAC 
CTCTTTAAGG AGGCTGTGCG GCAGGATAAC AAGCTCCTGC TGGAGAATCG ACCCAAATTC 
ATGCTGGTCC ACTCATCTTC AGGTCATAAG TATTCACTCA AAGAAATCCT GTCCGACCCC 
ACTGTGACGA GTAGACTCTC TGATACCAAG GCAGCTGGAG AGGTGAAAGC CCTGGAAGAC 
TTCTATAAGA TGCTCCAGCA TGAGCCTGAC AGAGCGTTCT ACGGAGTTGC TCATGTGGAG 
AAAGCTGCTG ACGCCCTCGC CATCGACACC TTGCTGATAA GTGATAAGTT GTTCAGGCAT 
CAGGATGTCC CCACAAGGAG TCGTTATGTT CGGTTGGTCG ATAACGTGAG AGACAATGGT 
GGCAATGTCA GGATATTCTC AAGCCTTCAC GTGTCTGGTG AACAACTGAC TCAGCTGAGC 
GGAGTGGCGG CCATCTTGAG GTTTCCCATC GCTGACCTAT CAGAAGCCGA GGATGAAAGC 
AGCTCAGACG ATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023126221.1
CDS	346..1503
Translation	MKLIHKDIEKDNAGQVTLVPEEAEDMWHTYNLLQVGDSLRASTIRKVQTESTTGSVGSSRVRTTLTICVETIDFDSHACQLRVKGTNIVENQYVKMGAYHTIELELNRKFTLAKKCWDSVVLDRIDQACDAAQKADVAAVVMQEGLANLVLVTPAMTLLRAKVEVTIPRKRRGSCTQHEKALERFYEAVMQAILRHINFDVVKCILVASPGFVKDQFITYLFKEAVRQDNKLLLENRPKFMLVHSSSGHKYSLKEILSDPTVTSRLSDTKAAGEVKALEDFYKMLQHEPDRAFYGVAHVEKAADALAIDTLLISDKLFRHQDVPTRSRYVRLVDNVRDNGGNVRIFSSLHVSGEQLTQLSGVAAILRFPIADLSEAEDESSSDDD

Target ORF information:

RefSeq Version	XM_023270453.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris pelota mRNA surveillance and ribosome rescue factor (pelo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023270453.1

ORF Insert Sequence:

ATGAAGCTGA TCCATAAAGA CATTGAAAAA GATAATGCCG GCCAGGTGAC TCTGGTGCCA 
GAGGAGGCGG AGGACATGTG GCACACCTAC AACCTGCTCC AAGTGGGGGA CAGCTTGAGA 
GCCTCAACTA TCAGAAAGGT GCAGACAGAG TCAACCACTG GAAGTGTGGG CAGCTCCAGA 
GTTCGGACCA CTCTAACAAT ATGTGTGGAG ACTATCGACT TTGACTCTCA TGCATGTCAG 
CTGAGAGTAA AAGGCACCAA CATAGTGGAG AACCAGTATG TCAAGATGGG GGCTTATCAC 
ACTATTGAGC TTGAGCTCAA TAGGAAGTTT ACTCTCGCTA AGAAGTGCTG GGACAGCGTT 
GTGCTGGATA GAATCGATCA AGCATGTGAT GCAGCCCAGA AGGCAGATGT GGCAGCTGTG 
GTGATGCAGG AGGGTCTGGC CAACCTGGTA CTGGTGACCC CGGCCATGAC TCTGCTTCGT 
GCAAAAGTGG AAGTCACTAT TCCTCGCAAG AGAAGGGGCA GCTGCACTCA GCACGAGAAG 
GCGCTGGAGA GGTTTTATGA GGCTGTGATG CAGGCGATAC TTCGCCACAT CAATTTTGAT 
GTGGTGAAGT GTATCCTGGT GGCGAGTCCA GGGTTTGTGA AGGACCAGTT TATCACCTAC 
CTCTTTAAGG AGGCTGTGCG GCAGGATAAC AAGCTCCTGC TGGAGAATCG ACCCAAATTC 
ATGCTGGTCC ACTCATCTTC AGGTCATAAG TATTCACTCA AAGAAATCCT GTCCGACCCC 
ACTGTGACGA GTAGACTCTC TGATACCAAG GCAGCTGGAG AGGTGAAAGC CCTGGAAGAC 
TTCTATAAGA TGCTCCAGCA TGAGCCTGAC AGAGCGTTCT ACGGAGTTGC TCATGTGGAG 
AAAGCTGCTG ACGCCCTCGC CATCGACACC TTGCTGATAA GTGATAAGTT GTTCAGGCAT 
CAGGATGTCC CCACAAGGAG TCGTTATGTT CGGTTGGTCG ATAACGTGAG AGACAATGGT 
GGCAATGTCA GGATATTCTC AAGCCTTCAC GTGTCTGGTG AACAACTGAC TCAGCTGAGC 
GGAGTGGCGG CCATCTTGAG GTTTCCCATC GCTGACCTAT CAGAAGCCGA GGATGAAAGC 
AGCTCAGACG ATGACTGA