cdc25b cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc25b
Entrez Gene ID	111568375
Full Name	cell division cycle 25B
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023270003.1	XP_023125771.1	M-phase inducer phosphatase 2

Pathways from BioSystems
Gene Ontology

The following cdc25b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc25b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm77658	XM_023270003.1 Latest version!	Amphiprion ocellaris cell division cycle 25B (cdc25b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc25b ( XM_023270003.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023125771.1 Amphiprion ocellaris cell division cycle 25B (cdc25b), mRNA.

Price & Availability↑

CloneID	OAm77658
Clone ID Related Accession (Same CDS sequence)	XM_023270003.1
Accession Version	XM_023270003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	M-phase inducer phosphatase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371999.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TTTATAGTCC CGTTAACACC ACGTCGAAAA GCAGGCCCGA AGGCATCCCC 
GGACTTTCCT CGCTCACTCT GCCCGAACTG AGCACAAAGG ACCCGCACTG CAGGAACCTA 
TTCTCCCCTG GACCCGCTGC TGTCCTCTCC CCTGTCACCA ATCTGGCTCT GGATATGAAC 
AATTTAGCTG GACTTGGAAG TCAATGTGAC ACCCCGAAAA GGAGAAAGCA TGCACCCCTT 
GAGAAGATTC CCTCCTTTGC TTCTGACGTC TCATCTGATG CTGGCCTCGG CATGGATCCC 
CCCAGCCCCA TGGATGCTGT CGAAGTTGAG GACACATTTG AAAAGGCCAT TCAGCAATCG 
AGCAGAGTAG TTAACGAGAA GATGCCAATT CGACGAATCA ACTCATTGCC ACTCCAGCTC 
CTGGGTTTCA GTCCATCTCT GAGGGGACAG GAAACCGATT CGAACCGCTA TGGAATATTC 
GGCCAACAAC CCTCTCAGAT AACCGCCTCC TCCGCTTCTG AACACGACAA GGAAAACATG 
CCAGAGGAGT GTTTTGAATT CAAGAAACCA ACCAAGCCGG CGTCACGTTG CCGCGTTCGC 
TCCTTCAACG GCGGACAGGC GAAGGACGCT TTTGCCCGCC GTCCCAGCTC AGCACCAGCC 
CTCATGCTTT GTTCGCCTCC TTCAGGCCAA CAGCAAGACT TCTTCGACTC CAGCCCCATG 
TTCCTGAGAC GCTCCTCCCT CACCTCGTCT CTAAACGACG ACGACGATGA TGACGGCTTT 
TTGGACGTAC TAGACGACAA CATGGAGAAT GACTCTGGGA TGCCGATGGG AATGGCCAGC 
TTGCTCACTG CCCCGCTGGT GTCCGACAGC ACAGCAGAAG ACTCGCCTGT GATTCGCTGC 
CGGCCACGCA ACCTGTTCCG CTCGCCCTCC ATGCCCAGTC CGGTGTCCCG TCCCGCCGTG 
AAGCGTCCCG ACTGCCCCGG AGATGAAAAC ACGCCGGTCA GGGTGAAGAG ACGACGCAGC 
CTGGCAGGAA CTCACGTCAC CATCCTGGAA CAAAACCCCG AATCCCCCAG AACGACCTGC 
TCGCTGCTGC AGAGGTCCAA ATCCTTCTGT CAGACGGAGA TTGAAAAGCT GCTGGACAGT 
GAGGACGGTT CTAATGAGCT AATAGGAGAT TTCACCAAGC CCTTCGTGCT CCCAACAGTG 
GAGGGAAAAC ATCAAGACCT AAAGTACATT ACCCCAGAAA TAATGGTGGC AGCTCTCACG 
GGACAGTTTG GTCATCTAGT TGAGCAAGTT ATCATCATTG ACTGCCGCTA CCCCTACGAG 
TTTGACGGAG GGCACATCAA GGGAGCTCTG AACCTGCACC AGGAAGACCA GGTGCAGGAT 
TTCCTCCTGA AGGCCCCCAT CGTCCCGGCC TGCCCCGACA AGCGAGTCGT CATCATCTTC 
CACTGCGAGT TCTCGTCGGA GCGCGGCCCT CGGATGTGCC GCTTCGTCCG GGAGCGAGAT 
CGCGCCATGA ACGACTACCC CAAACTCCAC TACCCCGAGC TCTACATCCT CAAGGGCGGG 
TACAAAGACT TCTTCCCGCA TTTTCAGGCA CAATGTGAAC CTCAGTCCTA CCGGCCGATG 
CACCACGAGG ACTTCAAGGA GGACCTGAGG AAGTTCCGCC TCAAAAGTCG CACCTGGGCC 
GGCGAGCGCA GCAAGAGAGA CATGTACAGC CGGCTGAAGA AGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023125771.1
CDS	330..2057
Translation	MEAFYSPVNTTSKSRPEGIPGLSSLTLPELSTKDPHCRNLFSPGPAAVLSPVTNLALDMNNLAGLGSQCDTPKRRKHAPLEKIPSFASDVSSDAGLGMDPPSPMDAVEVEDTFEKAIQQSSRVVNEKMPIRRINSLPLQLLGFSPSLRGQETDSNRYGIFGQQPSQITASSASEHDKENMPEECFEFKKPTKPASRCRVRSFNGGQAKDAFARRPSSAPALMLCSPPSGQQQDFFDSSPMFLRRSSLTSSLNDDDDDDGFLDVLDDNMENDSGMPMGMASLLTAPLVSDSTAEDSPVIRCRPRNLFRSPSMPSPVSRPAVKRPDCPGDENTPVRVKRRRSLAGTHVTILEQNPESPRTTCSLLQRSKSFCQTEIEKLLDSEDGSNELIGDFTKPFVLPTVEGKHQDLKYITPEIMVAALTGQFGHLVEQVIIIDCRYPYEFDGGHIKGALNLHQEDQVQDFLLKAPIVPACPDKRVVIIFHCEFSSERGPRMCRFVRERDRAMNDYPKLHYPELYILKGGYKDFFPHFQAQCEPQSYRPMHHEDFKEDLRKFRLKSRTWAGERSKRDMYSRLKKL

Target ORF information:

RefSeq Version	XM_023270003.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris cell division cycle 25B (cdc25b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023270003.1

ORF Insert Sequence:

ATGGAAGCCT TTTATAGTCC CGTTAACACC ACGTCGAAAA GCAGGCCCGA AGGCATCCCC 
GGACTTTCCT CGCTCACTCT GCCCGAACTG AGCACAAAGG ACCCGCACTG CAGGAACCTA 
TTCTCCCCTG GACCCGCTGC TGTCCTCTCC CCTGTCACCA ATCTGGCTCT GGATATGAAC 
AATTTAGCTG GACTTGGAAG TCAATGTGAC ACCCCGAAAA GGAGAAAGCA TGCACCCCTT 
GAGAAGATTC CCTCCTTTGC TTCTGACGTC TCATCTGATG CTGGCCTCGG CATGGATCCC 
CCCAGCCCCA TGGATGCTGT CGAAGTTGAG GACACATTTG AAAAGGCCAT TCAGCAATCG 
AGCAGAGTAG TTAACGAGAA GATGCCAATT CGACGAATCA ACTCATTGCC ACTCCAGCTC 
CTGGGTTTCA GTCCATCTCT GAGGGGACAG GAAACCGATT CGAACCGCTA TGGAATATTC 
GGCCAACAAC CCTCTCAGAT AACCGCCTCC TCCGCTTCTG AACACGACAA GGAAAACATG 
CCAGAGGAGT GTTTTGAATT CAAGAAACCA ACCAAGCCGG CGTCACGTTG CCGCGTTCGC 
TCCTTCAACG GCGGACAGGC GAAGGACGCT TTTGCCCGCC GTCCCAGCTC AGCACCAGCC 
CTCATGCTTT GTTCGCCTCC TTCAGGCCAA CAGCAAGACT TCTTCGACTC CAGCCCCATG 
TTCCTGAGAC GCTCCTCCCT CACCTCGTCT CTAAACGACG ACGACGATGA TGACGGCTTT 
TTGGACGTAC TAGACGACAA CATGGAGAAT GACTCTGGGA TGCCGATGGG AATGGCCAGC 
TTGCTCACTG CCCCGCTGGT GTCCGACAGC ACAGCAGAAG ACTCGCCTGT GATTCGCTGC 
CGGCCACGCA ACCTGTTCCG CTCGCCCTCC ATGCCCAGTC CGGTGTCCCG TCCCGCCGTG 
AAGCGTCCCG ACTGCCCCGG AGATGAAAAC ACGCCGGTCA GGGTGAAGAG ACGACGCAGC 
CTGGCAGGAA CTCACGTCAC CATCCTGGAA CAAAACCCCG AATCCCCCAG AACGACCTGC 
TCGCTGCTGC AGAGGTCCAA ATCCTTCTGT CAGACGGAGA TTGAAAAGCT GCTGGACAGT 
GAGGACGGTT CTAATGAGCT AATAGGAGAT TTCACCAAGC CCTTCGTGCT CCCAACAGTG 
GAGGGAAAAC ATCAAGACCT AAAGTACATT ACCCCAGAAA TAATGGTGGC AGCTCTCACG 
GGACAGTTTG GTCATCTAGT TGAGCAAGTT ATCATCATTG ACTGCCGCTA CCCCTACGAG 
TTTGACGGAG GGCACATCAA GGGAGCTCTG AACCTGCACC AGGAAGACCA GGTGCAGGAT 
TTCCTCCTGA AGGCCCCCAT CGTCCCGGCC TGCCCCGACA AGCGAGTCGT CATCATCTTC 
CACTGCGAGT TCTCGTCGGA GCGCGGCCCT CGGATGTGCC GCTTCGTCCG GGAGCGAGAT 
CGCGCCATGA ACGACTACCC CAAACTCCAC TACCCCGAGC TCTACATCCT CAAGGGCGGG 
TACAAAGACT TCTTCCCGCA TTTTCAGGCA CAATGTGAAC CTCAGTCCTA CCGGCCGATG 
CACCACGAGG ACTTCAAGGA GGACCTGAGG AAGTTCCGCC TCAAAAGTCG CACCTGGGCC 
GGCGAGCGCA GCAAGAGAGA CATGTACAGC CGGCTGAAGA AGCTGTGA