cars cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cars
Entrez Gene ID	111564412
Full Name	cysteinyl-tRNA synthetase
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023263958.1	XP_023119726.1	cysteine--tRNA ligase, cytoplasmic isoform X2
XM_023263957.1	XP_023119725.1	cysteine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems
Gene Ontology

The following cars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm103313	XM_023263957.1 Latest version!	Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAm103314	XM_023263958.1 Latest version!	Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cars ( XM_023263957.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119725.1 Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm103313
Clone ID Related Accession (Same CDS sequence)	XM_023263957.1
Accession Version	XM_023263957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	cysteine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376160.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCC CAGCAGAGCC AGCCTTTGAG TTTGGCTCCT TGCTGCAGAT CAATGAGGAG 
GCCGAGGCCC TGAACGACTA CCTGTCCTCA CGCAGCTACC TGGCCGGGTT CAGCCCCTCC 
CAGGCTGACC ACAAAGCCTT TAAGCTCCTC CGCAGACCCC CAGATCCACA CCACGTGCAC 
GCTCTGCGCT GGTACAGACA CATAGCAGCC CTGCAGCAGG ACCTCCACCC AGACAGCAGC 
ACTAAAGGGA AGCGAGTTCA GCCGTCCTGG TCTCCACCAG CAGGAACCGA CGTTCCTCAG 
CTCCGTCTCT ACAACAGCCT GACCAGAGCG AAGGAGCCGT TTGTTCCCCA GAGAGGTAAC 
AGGGTGACGT GGTACTGCTG CGGTCCTACC GTGTACGATG CGTCACACAT GGGTCACGCC 
AGATCCTACA TTTCCTTTGA CATCCTGCGG AGGACGCTGA GCGATTACTT CAAGTACGAC 
GTCTTCTACT GCATAAACAT CACCGACATC GACGACAAAA TCATTAAACG AGCTCGACAG 
AATTATCTTC TGGAGCAGTA CAAGGAGAAA AAACCTCAAC CTGCTCAGAT CCTGCAGGAC 
GTCCTGAGCG CCAGAGGGCC CTTTCAGGCC AGCCTGGCTT CGACCTCAGA CCCTGATAAG 
AAGCAGATGA TGGAGCGGCT GGACGCTGCT GTAGCCGACG CTCTGGAGCC CCTGCAGGGG 
GCGCTGGAGA GCAAAGCAGC AGATGTTCAA CCTCTGGCCC AGGCGCTGCT GGAGAGCTCC 
AAAGACCTGC TGGCCGACTG GTTGGACAAA CAGTTTGGAA GTCAAGTCAC AGAGAATTCA 
ATCTTCTCCA TCCTCCCCAA GTACTGGGAG GGAGAGTATC ATAAAGACAT GGAGGCCCTG 
AACGTTCTCC CTCCTGACGT CCTGACTCGG GTCAGTGAGT ACGTTCCTGA GATCGTGGAG 
TTTGTGAAGA AGATCGTCTC CAACGGTTAC GGGTACGAGT CCAACGGCTC GGTTTACTTC 
AACACCTCTA AGTTTGACTC CAGCCCACAG CACTCATACG CCAAGCTGGT TCCGGAGGCC 
GTGGGAGACC AGAAAGCTCT GCAGGAGGGA GAAGGTGACC TGAGCATCTC TGCTGACAGG 
TTAAGTGAGA AGAAGTCCCA GAATGACTTT GCCTTGTGGA AAGCCTCCAA ACCTGGAGAA 
CCTTCCTGGG ACTCTCCATG GGGGAAGGGG CGTCCTGGAT GGCACATCGA ATGTTCAGCC 
ATGGCCGGCT CCATCCTGGG GGAGTCGATG GACATCCACG GCGGAGGGTT TGATCTCCGG 
TTCCCTCATC ACGACAACGA GCTGGCTCAG TCTGAGGCGT TCTTTGAGAA CGATCACTGG 
GTGCGTTACT TCCTCCACAC CGGACATCTG ACCATCGCTG GCTGTAAGAT GTCCAAGTCT 
CTGAAGAACT TCATCACCAT TAAGGACGCC TTGGCCAAGA ACACAGCCCG GCAGCTCCGT 
CTGGCGTTCC TCATGCATTC CTGGAAGGAC ACTCTGGACT ATTCCTCCAA CACCATGGAG 
TCGGCCGTCC AGTACGAGAA GTTCATGAAC GAGTTCTTCC TGAACGTGAA GGACATCCTG 
AGAGCTCCTA CCGATGTCAC CGGTCAGTTT GAGAAGTGGG AGGAAGCCGA GGTGGAGCTC 
AACAACAGTT TCTACGACAG GAAAGCTGCC GTCCACGAGG CTCTCTGCGA CAACATCGAC 
ACCCGGACCG CCATGGAGGA GATGAGGGTT CTGGTGGGTC AGAGCAACAG CTACGTCGCC 
AGCAGGAAGA ACGCCAAGCT GAGGCCGAAC CGGCTGCTGA TGGAGAGCAT CGCCGTCTAC 
CTCACCAACA TGCTGAAGAT ATTTGGTGCC ATTGAAGGAT CCGACCCGAT TGGTTTCCCT 
GTTGGAGGAC AGGATCAGAG CGTGGATCTG GAGAGCACCG TGATGCCGTA CCTCTCCGTG 
CTGTCGGACT TCAGGGAGAA CGTACGGAAA ATCGCCCGAG AGCAGAAAGT GATGGAGCTG 
CTGCAGCTCT GCGACGATGT CCGTGACGAA ACATTACCAG AGCTGGGAGT CCGACTGGAA 
GATCATGAAG GTTTGCCTAC TGTGGTGAAA CTGGTGGACA AGGAGACCTT ACTGAAGGAG 
AAAGAGGAGA AGAAGAAGAT TGAAGAGGAA AAGAAAAAGA AGAAGGAAGA AGCTGCCAGG 
AAGAAACAGG AACAGGAGAT GGCCAAACTG GCCAAGATGA AGACGCCTCC ATGTGAGATG 
TTTCGCTGTG AAACGGACAA ATATTCCAGT TTTGATGAAA CGGGTTTTCC CACCCATGAT 
GCTGAAGGGA AGGAGCTCAG TAAAGGACAA GCCAAGAAGC TGCGTAAACT TTACGAGGCT 
CAGGAGAAGC TTCACAGCGA GTATCTTCAG ATGAACCAGA ACGGAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119725.1
CDS	84..2534
Translation	MSTPAEPAFEFGSLLQINEEAEALNDYLSSRSYLAGFSPSQADHKAFKLLRRPPDPHHVHALRWYRHIAALQQDLHPDSSTKGKRVQPSWSPPAGTDVPQLRLYNSLTRAKEPFVPQRGNRVTWYCCGPTVYDASHMGHARSYISFDILRRTLSDYFKYDVFYCINITDIDDKIIKRARQNYLLEQYKEKKPQPAQILQDVLSARGPFQASLASTSDPDKKQMMERLDAAVADALEPLQGALESKAADVQPLAQALLESSKDLLADWLDKQFGSQVTENSIFSILPKYWEGEYHKDMEALNVLPPDVLTRVSEYVPEIVEFVKKIVSNGYGYESNGSVYFNTSKFDSSPQHSYAKLVPEAVGDQKALQEGEGDLSISADRLSEKKSQNDFALWKASKPGEPSWDSPWGKGRPGWHIECSAMAGSILGESMDIHGGGFDLRFPHHDNELAQSEAFFENDHWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALAKNTARQLRLAFLMHSWKDTLDYSSNTMESAVQYEKFMNEFFLNVKDILRAPTDVTGQFEKWEEAEVELNNSFYDRKAAVHEALCDNIDTRTAMEEMRVLVGQSNSYVASRKNAKLRPNRLLMESIAVYLTNMLKIFGAIEGSDPIGFPVGGQDQSVDLESTVMPYLSVLSDFRENVRKIAREQKVMELLQLCDDVRDETLPELGVRLEDHEGLPTVVKLVDKETLLKEKEEKKKIEEEKKKKKEEAARKKQEQEMAKLAKMKTPPCEMFRCETDKYSSFDETGFPTHDAEGKELSKGQAKKLRKLYEAQEKLHSEYLQMNQNGN

Target ORF information:

RefSeq Version	XM_023263957.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263957.1

ORF Insert Sequence:

ATGTCGACCC CAGCAGAGCC AGCCTTTGAG TTTGGCTCCT TGCTGCAGAT CAATGAGGAG 
GCCGAGGCCC TGAACGACTA CCTGTCCTCA CGCAGCTACC TGGCCGGGTT CAGCCCCTCC 
CAGGCTGACC ACAAAGCCTT TAAGCTCCTC CGCAGACCCC CAGATCCACA CCACGTGCAC 
GCTCTGCGCT GGTACAGACA CATAGCAGCC CTGCAGCAGG ACCTCCACCC AGACAGCAGC 
ACTAAAGGGA AGCGAGTTCA GCCGTCCTGG TCTCCACCAG CAGGAACCGA CGTTCCTCAG 
CTCCGTCTCT ACAACAGCCT GACCAGAGCG AAGGAGCCGT TTGTTCCCCA GAGAGGTAAC 
AGGGTGACGT GGTACTGCTG CGGTCCTACC GTGTACGATG CGTCACACAT GGGTCACGCC 
AGATCCTACA TTTCCTTTGA CATCCTGCGG AGGACGCTGA GCGATTACTT CAAGTACGAC 
GTCTTCTACT GCATAAACAT CACCGACATC GACGACAAAA TCATTAAACG AGCTCGACAG 
AATTATCTTC TGGAGCAGTA CAAGGAGAAA AAACCTCAAC CTGCTCAGAT CCTGCAGGAC 
GTCCTGAGCG CCAGAGGGCC CTTTCAGGCC AGCCTGGCTT CGACCTCAGA CCCTGATAAG 
AAGCAGATGA TGGAGCGGCT GGACGCTGCT GTAGCCGACG CTCTGGAGCC CCTGCAGGGG 
GCGCTGGAGA GCAAAGCAGC AGATGTTCAA CCTCTGGCCC AGGCGCTGCT GGAGAGCTCC 
AAAGACCTGC TGGCCGACTG GTTGGACAAA CAGTTTGGAA GTCAAGTCAC AGAGAATTCA 
ATCTTCTCCA TCCTCCCCAA GTACTGGGAG GGAGAGTATC ATAAAGACAT GGAGGCCCTG 
AACGTTCTCC CTCCTGACGT CCTGACTCGG GTCAGTGAGT ACGTTCCTGA GATCGTGGAG 
TTTGTGAAGA AGATCGTCTC CAACGGTTAC GGGTACGAGT CCAACGGCTC GGTTTACTTC 
AACACCTCTA AGTTTGACTC CAGCCCACAG CACTCATACG CCAAGCTGGT TCCGGAGGCC 
GTGGGAGACC AGAAAGCTCT GCAGGAGGGA GAAGGTGACC TGAGCATCTC TGCTGACAGG 
TTAAGTGAGA AGAAGTCCCA GAATGACTTT GCCTTGTGGA AAGCCTCCAA ACCTGGAGAA 
CCTTCCTGGG ACTCTCCATG GGGGAAGGGG CGTCCTGGAT GGCACATCGA ATGTTCAGCC 
ATGGCCGGCT CCATCCTGGG GGAGTCGATG GACATCCACG GCGGAGGGTT TGATCTCCGG 
TTCCCTCATC ACGACAACGA GCTGGCTCAG TCTGAGGCGT TCTTTGAGAA CGATCACTGG 
GTGCGTTACT TCCTCCACAC CGGACATCTG ACCATCGCTG GCTGTAAGAT GTCCAAGTCT 
CTGAAGAACT TCATCACCAT TAAGGACGCC TTGGCCAAGA ACACAGCCCG GCAGCTCCGT 
CTGGCGTTCC TCATGCATTC CTGGAAGGAC ACTCTGGACT ATTCCTCCAA CACCATGGAG 
TCGGCCGTCC AGTACGAGAA GTTCATGAAC GAGTTCTTCC TGAACGTGAA GGACATCCTG 
AGAGCTCCTA CCGATGTCAC CGGTCAGTTT GAGAAGTGGG AGGAAGCCGA GGTGGAGCTC 
AACAACAGTT TCTACGACAG GAAAGCTGCC GTCCACGAGG CTCTCTGCGA CAACATCGAC 
ACCCGGACCG CCATGGAGGA GATGAGGGTT CTGGTGGGTC AGAGCAACAG CTACGTCGCC 
AGCAGGAAGA ACGCCAAGCT GAGGCCGAAC CGGCTGCTGA TGGAGAGCAT CGCCGTCTAC 
CTCACCAACA TGCTGAAGAT ATTTGGTGCC ATTGAAGGAT CCGACCCGAT TGGTTTCCCT 
GTTGGAGGAC AGGATCAGAG CGTGGATCTG GAGAGCACCG TGATGCCGTA CCTCTCCGTG 
CTGTCGGACT TCAGGGAGAA CGTACGGAAA ATCGCCCGAG AGCAGAAAGT GATGGAGCTG 
CTGCAGCTCT GCGACGATGT CCGTGACGAA ACATTACCAG AGCTGGGAGT CCGACTGGAA 
GATCATGAAG GTTTGCCTAC TGTGGTGAAA CTGGTGGACA AGGAGACCTT ACTGAAGGAG 
AAAGAGGAGA AGAAGAAGAT TGAAGAGGAA AAGAAAAAGA AGAAGGAAGA AGCTGCCAGG 
AAGAAACAGG AACAGGAGAT GGCCAAACTG GCCAAGATGA AGACGCCTCC ATGTGAGATG 
TTTCGCTGTG AAACGGACAA ATATTCCAGT TTTGATGAAA CGGGTTTTCC CACCCATGAT 
GCTGAAGGGA AGGAGCTCAG TAAAGGACAA GCCAAGAAGC TGCGTAAACT TTACGAGGCT 
CAGGAGAAGC TTCACAGCGA GTATCTTCAG ATGAACCAGA ACGGAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cars ( XM_023263958.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119726.1 Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm103314
Clone ID Related Accession (Same CDS sequence)	XM_023263958.1
Accession Version	XM_023263958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	cysteine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376160.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCC CAGCAGAGCC AGCTAAAGGG AAGCGAGTTC AGCCGTCCTG GTCTCCACCA 
GCAGGAACCG ACGTTCCTCA GCTCCGTCTC TACAACAGCC TGACCAGAGC GAAGGAGCCG 
TTTGTTCCCC AGAGAGGTAA CAGGGTGACG TGGTACTGCT GCGGTCCTAC CGTGTACGAT 
GCGTCACACA TGGGTCACGC CAGATCCTAC ATTTCCTTTG ACATCCTGCG GAGGACGCTG 
AGCGATTACT TCAAGTACGA CGTCTTCTAC TGCATAAACA TCACCGACAT CGACGACAAA 
ATCATTAAAC GAGCTCGACA GAATTATCTT CTGGAGCAGT ACAAGGAGAA AAAACCTCAA 
CCTGCTCAGA TCCTGCAGGA CGTCCTGAGC GCCAGAGGGC CCTTTCAGGC CAGCCTGGCT 
TCGACCTCAG ACCCTGATAA GAAGCAGATG ATGGAGCGGC TGGACGCTGC TGTAGCCGAC 
GCTCTGGAGC CCCTGCAGGG GGCGCTGGAG AGCAAAGCAG CAGATGTTCA ACCTCTGGCC 
CAGGCGCTGC TGGAGAGCTC CAAAGACCTG CTGGCCGACT GGTTGGACAA ACAGTTTGGA 
AGTCAAGTCA CAGAGAATTC AATCTTCTCC ATCCTCCCCA AGTACTGGGA GGGAGAGTAT 
CATAAAGACA TGGAGGCCCT GAACGTTCTC CCTCCTGACG TCCTGACTCG GGTCAGTGAG 
TACGTTCCTG AGATCGTGGA GTTTGTGAAG AAGATCGTCT CCAACGGTTA CGGGTACGAG 
TCCAACGGCT CGGTTTACTT CAACACCTCT AAGTTTGACT CCAGCCCACA GCACTCATAC 
GCCAAGCTGG TTCCGGAGGC CGTGGGAGAC CAGAAAGCTC TGCAGGAGGG AGAAGGTGAC 
CTGAGCATCT CTGCTGACAG GTTAAGTGAG AAGAAGTCCC AGAATGACTT TGCCTTGTGG 
AAAGCCTCCA AACCTGGAGA ACCTTCCTGG GACTCTCCAT GGGGGAAGGG GCGTCCTGGA 
TGGCACATCG AATGTTCAGC CATGGCCGGC TCCATCCTGG GGGAGTCGAT GGACATCCAC 
GGCGGAGGGT TTGATCTCCG GTTCCCTCAT CACGACAACG AGCTGGCTCA GTCTGAGGCG 
TTCTTTGAGA ACGATCACTG GGTGCGTTAC TTCCTCCACA CCGGACATCT GACCATCGCT 
GGCTGTAAGA TGTCCAAGTC TCTGAAGAAC TTCATCACCA TTAAGGACGC CTTGGCCAAG 
AACACAGCCC GGCAGCTCCG TCTGGCGTTC CTCATGCATT CCTGGAAGGA CACTCTGGAC 
TATTCCTCCA ACACCATGGA GTCGGCCGTC CAGTACGAGA AGTTCATGAA CGAGTTCTTC 
CTGAACGTGA AGGACATCCT GAGAGCTCCT ACCGATGTCA CCGGTCAGTT TGAGAAGTGG 
GAGGAAGCCG AGGTGGAGCT CAACAACAGT TTCTACGACA GGAAAGCTGC CGTCCACGAG 
GCTCTCTGCG ACAACATCGA CACCCGGACC GCCATGGAGG AGATGAGGGT TCTGGTGGGT 
CAGAGCAACA GCTACGTCGC CAGCAGGAAG AACGCCAAGC TGAGGCCGAA CCGGCTGCTG 
ATGGAGAGCA TCGCCGTCTA CCTCACCAAC ATGCTGAAGA TATTTGGTGC CATTGAAGGA 
TCCGACCCGA TTGGTTTCCC TGTTGGAGGA CAGGATCAGA GCGTGGATCT GGAGAGCACC 
GTGATGCCGT ACCTCTCCGT GCTGTCGGAC TTCAGGGAGA ACGTACGGAA AATCGCCCGA 
GAGCAGAAAG TGATGGAGCT GCTGCAGCTC TGCGACGATG TCCGTGACGA AACATTACCA 
GAGCTGGGAG TCCGACTGGA AGATCATGAA GGTTTGCCTA CTGTGGTGAA ACTGGTGGAC 
AAGGAGACCT TACTGAAGGA GAAAGAGGAG AAGAAGAAGA TTGAAGAGGA AAAGAAAAAG 
AAGAAGGAAG AAGCTGCCAG GAAGAAACAG GAACAGGAGA TGGCCAAACT GGCCAAGATG 
AAGACGCCTC CATGTGAGAT GTTTCGCTGT GAAACGGACA AATATTCCAG TTTTGATGAA 
ACGGGTTTTC CCACCCATGA TGCTGAAGGG AAGGAGCTCA GTAAAGGACA AGCCAAGAAG 
CTGCGTAAAC TTTACGAGGC TCAGGAGAAG CTTCACAGCG AGTATCTTCA GATGAACCAG 
AACGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119726.1
CDS	105..2336
Translation	MSTPAEPAKGKRVQPSWSPPAGTDVPQLRLYNSLTRAKEPFVPQRGNRVTWYCCGPTVYDASHMGHARSYISFDILRRTLSDYFKYDVFYCINITDIDDKIIKRARQNYLLEQYKEKKPQPAQILQDVLSARGPFQASLASTSDPDKKQMMERLDAAVADALEPLQGALESKAADVQPLAQALLESSKDLLADWLDKQFGSQVTENSIFSILPKYWEGEYHKDMEALNVLPPDVLTRVSEYVPEIVEFVKKIVSNGYGYESNGSVYFNTSKFDSSPQHSYAKLVPEAVGDQKALQEGEGDLSISADRLSEKKSQNDFALWKASKPGEPSWDSPWGKGRPGWHIECSAMAGSILGESMDIHGGGFDLRFPHHDNELAQSEAFFENDHWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALAKNTARQLRLAFLMHSWKDTLDYSSNTMESAVQYEKFMNEFFLNVKDILRAPTDVTGQFEKWEEAEVELNNSFYDRKAAVHEALCDNIDTRTAMEEMRVLVGQSNSYVASRKNAKLRPNRLLMESIAVYLTNMLKIFGAIEGSDPIGFPVGGQDQSVDLESTVMPYLSVLSDFRENVRKIAREQKVMELLQLCDDVRDETLPELGVRLEDHEGLPTVVKLVDKETLLKEKEEKKKIEEEKKKKKEEAARKKQEQEMAKLAKMKTPPCEMFRCETDKYSSFDETGFPTHDAEGKELSKGQAKKLRKLYEAQEKLHSEYLQMNQNGN

Target ORF information:

RefSeq Version	XM_023263958.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris cysteinyl-tRNA synthetase (cars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263958.1

ORF Insert Sequence:

ATGTCGACCC CAGCAGAGCC AGCTAAAGGG AAGCGAGTTC AGCCGTCCTG GTCTCCACCA 
GCAGGAACCG ACGTTCCTCA GCTCCGTCTC TACAACAGCC TGACCAGAGC GAAGGAGCCG 
TTTGTTCCCC AGAGAGGTAA CAGGGTGACG TGGTACTGCT GCGGTCCTAC CGTGTACGAT 
GCGTCACACA TGGGTCACGC CAGATCCTAC ATTTCCTTTG ACATCCTGCG GAGGACGCTG 
AGCGATTACT TCAAGTACGA CGTCTTCTAC TGCATAAACA TCACCGACAT CGACGACAAA 
ATCATTAAAC GAGCTCGACA GAATTATCTT CTGGAGCAGT ACAAGGAGAA AAAACCTCAA 
CCTGCTCAGA TCCTGCAGGA CGTCCTGAGC GCCAGAGGGC CCTTTCAGGC CAGCCTGGCT 
TCGACCTCAG ACCCTGATAA GAAGCAGATG ATGGAGCGGC TGGACGCTGC TGTAGCCGAC 
GCTCTGGAGC CCCTGCAGGG GGCGCTGGAG AGCAAAGCAG CAGATGTTCA ACCTCTGGCC 
CAGGCGCTGC TGGAGAGCTC CAAAGACCTG CTGGCCGACT GGTTGGACAA ACAGTTTGGA 
AGTCAAGTCA CAGAGAATTC AATCTTCTCC ATCCTCCCCA AGTACTGGGA GGGAGAGTAT 
CATAAAGACA TGGAGGCCCT GAACGTTCTC CCTCCTGACG TCCTGACTCG GGTCAGTGAG 
TACGTTCCTG AGATCGTGGA GTTTGTGAAG AAGATCGTCT CCAACGGTTA CGGGTACGAG 
TCCAACGGCT CGGTTTACTT CAACACCTCT AAGTTTGACT CCAGCCCACA GCACTCATAC 
GCCAAGCTGG TTCCGGAGGC CGTGGGAGAC CAGAAAGCTC TGCAGGAGGG AGAAGGTGAC 
CTGAGCATCT CTGCTGACAG GTTAAGTGAG AAGAAGTCCC AGAATGACTT TGCCTTGTGG 
AAAGCCTCCA AACCTGGAGA ACCTTCCTGG GACTCTCCAT GGGGGAAGGG GCGTCCTGGA 
TGGCACATCG AATGTTCAGC CATGGCCGGC TCCATCCTGG GGGAGTCGAT GGACATCCAC 
GGCGGAGGGT TTGATCTCCG GTTCCCTCAT CACGACAACG AGCTGGCTCA GTCTGAGGCG 
TTCTTTGAGA ACGATCACTG GGTGCGTTAC TTCCTCCACA CCGGACATCT GACCATCGCT 
GGCTGTAAGA TGTCCAAGTC TCTGAAGAAC TTCATCACCA TTAAGGACGC CTTGGCCAAG 
AACACAGCCC GGCAGCTCCG TCTGGCGTTC CTCATGCATT CCTGGAAGGA CACTCTGGAC 
TATTCCTCCA ACACCATGGA GTCGGCCGTC CAGTACGAGA AGTTCATGAA CGAGTTCTTC 
CTGAACGTGA AGGACATCCT GAGAGCTCCT ACCGATGTCA CCGGTCAGTT TGAGAAGTGG 
GAGGAAGCCG AGGTGGAGCT CAACAACAGT TTCTACGACA GGAAAGCTGC CGTCCACGAG 
GCTCTCTGCG ACAACATCGA CACCCGGACC GCCATGGAGG AGATGAGGGT TCTGGTGGGT 
CAGAGCAACA GCTACGTCGC CAGCAGGAAG AACGCCAAGC TGAGGCCGAA CCGGCTGCTG 
ATGGAGAGCA TCGCCGTCTA CCTCACCAAC ATGCTGAAGA TATTTGGTGC CATTGAAGGA 
TCCGACCCGA TTGGTTTCCC TGTTGGAGGA CAGGATCAGA GCGTGGATCT GGAGAGCACC 
GTGATGCCGT ACCTCTCCGT GCTGTCGGAC TTCAGGGAGA ACGTACGGAA AATCGCCCGA 
GAGCAGAAAG TGATGGAGCT GCTGCAGCTC TGCGACGATG TCCGTGACGA AACATTACCA 
GAGCTGGGAG TCCGACTGGA AGATCATGAA GGTTTGCCTA CTGTGGTGAA ACTGGTGGAC 
AAGGAGACCT TACTGAAGGA GAAAGAGGAG AAGAAGAAGA TTGAAGAGGA AAAGAAAAAG 
AAGAAGGAAG AAGCTGCCAG GAAGAAACAG GAACAGGAGA TGGCCAAACT GGCCAAGATG 
AAGACGCCTC CATGTGAGAT GTTTCGCTGT GAAACGGACA AATATTCCAG TTTTGATGAA 
ACGGGTTTTC CCACCCATGA TGCTGAAGGG AAGGAGCTCA GTAAAGGACA AGCCAAGAAG 
CTGCGTAAAC TTTACGAGGC TCAGGAGAAG CTTCACAGCG AGTATCTTCA GATGAACCAG 
AACGGAAATT GA