LOC18431955 cDNA ORF clone, Amborella trichopoda

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18431955
Entrez Gene ID	18431955
Full Name	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Gene Type	protein-coding
Organism	Amborella trichopoda

mRNA and Protein(s)

mRNA	Protein	Name
XM_020665787.1	XP_020521446.1	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X3
XM_011624203.2	XP_011622505.2	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X1
XM_011624202.2	XP_011622504.2	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X2
XM_011624204.2	XP_011622506.2	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC18431955 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18431955 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAm46254	XM_011624203.2 Latest version!	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAm46255	XM_011624202.2 Latest version!	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAm46256	XM_020665787.1 Latest version!	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAm46257	XM_011624204.2 Latest version!	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18431955 ( XM_011624203.2 ) cDNA ORF clone, Amborella trichopoda -> XP_011622505.2 Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm46254
Clone ID Related Accession (Same CDS sequence)	XM_011624203.2
Accession Version	XM_011624203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-03
Organism	Amborella trichopoda
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006498085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 4, 2017 this sequence version replaced XM_011624203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amborella trichopoda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACGT 
AAGTCGGAGC AACCCTTTTA CCTGGACTGG GCTGTACATG CTTTTAGAAT CACCAACTAT 
GGTGTGAAAG ACACTACCCA GATTCACACA CACATGTGCT ACTCCAACTT CAACGACATA 
ATTCTCTCTA TCATGGACAT GGATGCTGAT GTGATTACAA TCGAGAATTC AAGATCCAAC 
GAGAAGCTCT TATCAGTGTT TAGAGAGGGC ATCAAGTATG GAGCAGGGAT TGGGCCTGGA 
GTGTATGATA TACATTCACC AAGAATCCCA ACCACCGAGG AGATAGAGGA GAGGGTTAGA 
AAGATGTTGG GTGTGCTTGA GCCGAGTATG GTATGGGTGA ACCCAGACTG TGGTCTCAAG 
ACCAGGAAGT ACTCTGAGGT GATTCCTTCT CTAAGCAACA TGGTTGCTGC TACAAAGCTT 
CTCCGAATTG AACTGGCCGA TACCAACAGG TGTTTTGAGT TGGGGGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011622505.2
CDS	77..2527
Translation	MFPPMMSCLKPSFLSLKLRSSSLFSVHGYLRHRPVKIHSFIRSMASHVVGYPRIGPKRELKFALESFWDGRSSAEDLQKVAKELRHSMWKQMSDVGIKYIPCNTFSYYDHVLDTTAMLGAVPPRYGWMGGEIGFDVYFSMARGNATLPAMEMTKWFDTNYHYLVPELGPDIKFSYSSHKAVSEYKEAKELGFETVPVLVGPVSYLLLSKPTKSVDRPFSLLSLLGKIIPIYKEVLAELKAAGAKWVQFDEPTLVLDLDSHQRKAFTEAYTELEESFSGLRVVVETYFADVPAEAYKTIIALKGISGIGFDLVRGEKTLDLIQNVGFPSGKFLIAGVVDGRNIWASDLGASMSILKALEGIVGKEKLVVSTSCSLLHTAVDLVNETKLDSEIKSWLAFAAQKILEVSTLAKALAGKKDEAFFMANAAAISSRKSSSRVNNEAVKISALALKGSDHHRATPVSTRLDVQQKELNLPILPTTTIGSFPQTMDLRRVRREYKANNITEEEYMRAIKEEICKVVKLQEDLGIDVLVHGEPERNDMVEYFGEQLHGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPRPMTIFWSKAAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKNEVEDLEDAGIQVIQIDEAALREGLPLRKSEQPFYLDWAVHAFRITNYGVKDTTQIHTHMCYSNFNDIILSIMDMDADVITIENSRSNEKLLSVFREGIKYGAGIGPGVYDIHSPRIPTTEEIEERVRKMLGVLEPSMVWVNPDCGLKTRKYSEVIPSLSNMVAATKLLRIELADTNRCFELGA

Target ORF information:

RefSeq Version	XM_011624203.2
Organism	Amborella trichopoda
Definition	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011624203.2

ORF Insert Sequence:

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACGT 
AAGTCGGAGC AACCCTTTTA CCTGGACTGG GCTGTACATG CTTTTAGAAT CACCAACTAT 
GGTGTGAAAG ACACTACCCA GATTCACACA CACATGTGCT ACTCCAACTT CAACGACATA 
ATTCTCTCTA TCATGGACAT GGATGCTGAT GTGATTACAA TCGAGAATTC AAGATCCAAC 
GAGAAGCTCT TATCAGTGTT TAGAGAGGGC ATCAAGTATG GAGCAGGGAT TGGGCCTGGA 
GTGTATGATA TACATTCACC AAGAATCCCA ACCACCGAGG AGATAGAGGA GAGGGTTAGA 
AAGATGTTGG GTGTGCTTGA GCCGAGTATG GTATGGGTGA ACCCAGACTG TGGTCTCAAG 
ACCAGGAAGT ACTCTGAGGT GATTCCTTCT CTAAGCAACA TGGTTGCTGC TACAAAGCTT 
CTCCGAATTG AACTGGCCGA TACCAACAGG TGTTTTGAGT TGGGGGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18431955 ( XM_011624202.2 ) cDNA ORF clone, Amborella trichopoda -> XP_011622504.2 Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm46255
Clone ID Related Accession (Same CDS sequence)	XM_011624202.2
Accession Version	XM_011624202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-03
Organism	Amborella trichopoda
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006498085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 4, 2017 this sequence version replaced XM_011624202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amborella trichopoda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACGT 
AAGTCGGAGC AACCCTTTTA CCTGGACTGG GCTGTACATG CTTTTAGAAT CACCAACTAT 
GGTGTGAAAG ACACTACCCA GATTCACACA CACATGTGCT ACTCCAACTT CAACGACATA 
ATTCTCTCTA TCATGGACAT GGATGCTGAT GTGATTACAA TCGAGAATTC AAGATCCAAC 
GAGAAGCTCT TATCAGTGTT TAGAGAGGGC ATCAAGTATG GAGCAGGGAT TGGGCCTGGA 
GTGTATGATA TACATTCACC AAGAATCCCA ACCACCGAGG AGATAGAGGA GAGGGTTAGA 
AAGATGTTGG GTGTGCTTGA GCCGAGTATG GTATGGGTGA ACCCAGACTG TGGTCTCAAG 
ACCAGGAAGT ACTCTGAGGT GATTCCTTCT CTAAGCAACA TGGTTGCTGC TACAAAGCTT 
CTCCGAATTG AACTGGCCGA TACCAAGTGT TTTGAGTTGG GGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011622504.2
CDS	76..2523
Translation	MFPPMMSCLKPSFLSLKLRSSSLFSVHGYLRHRPVKIHSFIRSMASHVVGYPRIGPKRELKFALESFWDGRSSAEDLQKVAKELRHSMWKQMSDVGIKYIPCNTFSYYDHVLDTTAMLGAVPPRYGWMGGEIGFDVYFSMARGNATLPAMEMTKWFDTNYHYLVPELGPDIKFSYSSHKAVSEYKEAKELGFETVPVLVGPVSYLLLSKPTKSVDRPFSLLSLLGKIIPIYKEVLAELKAAGAKWVQFDEPTLVLDLDSHQRKAFTEAYTELEESFSGLRVVVETYFADVPAEAYKTIIALKGISGIGFDLVRGEKTLDLIQNVGFPSGKFLIAGVVDGRNIWASDLGASMSILKALEGIVGKEKLVVSTSCSLLHTAVDLVNETKLDSEIKSWLAFAAQKILEVSTLAKALAGKKDEAFFMANAAAISSRKSSSRVNNEAVKISALALKGSDHHRATPVSTRLDVQQKELNLPILPTTTIGSFPQTMDLRRVRREYKANNITEEEYMRAIKEEICKVVKLQEDLGIDVLVHGEPERNDMVEYFGEQLHGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPRPMTIFWSKAAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKNEVEDLEDAGIQVIQIDEAALREGLPLRKSEQPFYLDWAVHAFRITNYGVKDTTQIHTHMCYSNFNDIILSIMDMDADVITIENSRSNEKLLSVFREGIKYGAGIGPGVYDIHSPRIPTTEEIEERVRKMLGVLEPSMVWVNPDCGLKTRKYSEVIPSLSNMVAATKLLRIELADTKCFELGA

Target ORF information:

RefSeq Version	XM_011624202.2
Organism	Amborella trichopoda
Definition	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011624202.2

ORF Insert Sequence:

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACGT 
AAGTCGGAGC AACCCTTTTA CCTGGACTGG GCTGTACATG CTTTTAGAAT CACCAACTAT 
GGTGTGAAAG ACACTACCCA GATTCACACA CACATGTGCT ACTCCAACTT CAACGACATA 
ATTCTCTCTA TCATGGACAT GGATGCTGAT GTGATTACAA TCGAGAATTC AAGATCCAAC 
GAGAAGCTCT TATCAGTGTT TAGAGAGGGC ATCAAGTATG GAGCAGGGAT TGGGCCTGGA 
GTGTATGATA TACATTCACC AAGAATCCCA ACCACCGAGG AGATAGAGGA GAGGGTTAGA 
AAGATGTTGG GTGTGCTTGA GCCGAGTATG GTATGGGTGA ACCCAGACTG TGGTCTCAAG 
ACCAGGAAGT ACTCTGAGGT GATTCCTTCT CTAAGCAACA TGGTTGCTGC TACAAAGCTT 
CTCCGAATTG AACTGGCCGA TACCAAGTGT TTTGAGTTGG GGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18431955 ( XM_020665787.1 ) cDNA ORF clone, Amborella trichopoda -> XP_020521446.1 Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X3, mRNA.

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CloneID	OAm46256
Clone ID Related Accession (Same CDS sequence)	XM_020665787.1
Accession Version	XM_020665787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-03
Organism	Amborella trichopoda
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006498085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amborella trichopoda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC ATGTTGTTGG GTATCCGCGA ATTGGACCAA AGAGGGAGCT CAAGTTTGCT 
CTCGAGTCCT TTTGGGATGG CAGAAGTAGT GCTGAGGATT TGCAGAAGGT TGCAAAAGAG 
CTGAGGCATT CTATGTGGAA ACAAATGTCA GATGTGGGTA TAAAATATAT ACCCTGCAAT 
ACATTTTCAT ACTATGATCA TGTCCTAGAT ACAACTGCAA TGCTTGGTGC AGTTCCTCCA 
AGATATGGAT GGATGGGAGG TGAGATTGGT TTTGATGTTT ATTTCTCCAT GGCCAGAGGG 
AATGCTACTC TCCCTGCCAT GGAAATGACA AAATGGTTTG ATACCAACTA TCACTATCTT 
GTACCGGAAT TGGGACCTGA CATTAAATTC TCATATTCTT CTCACAAAGC TGTGTCTGAA 
TACAAGGAGG CAAAGGAGCT TGGATTTGAG ACTGTTCCAG TACTTGTGGG CCCCGTGTCT 
TACTTGTTGC TATCAAAACC CACAAAGAGT GTAGACAGAC CATTTTCTCT TCTTTCATTG 
TTGGGGAAGA TCATCCCTAT TTACAAGGAG GTTTTGGCTG AACTAAAGGC AGCTGGTGCG 
AAGTGGGTGC AGTTTGATGA GCCGACGCTC GTGTTAGATC TGGACTCTCA CCAACGAAAA 
GCATTTACTG AGGCCTATAC AGAGTTGGAA GAATCATTTT CTGGTTTGAG AGTGGTTGTC 
GAGACCTACT TTGCTGATGT GCCTGCTGAG GCATACAAGA CTATCATAGC ATTGAAGGGT 
ATCTCAGGTA TTGGTTTTGA TCTGGTGCGT GGAGAAAAGA CCCTTGATTT GATCCAAAAT 
GTAGGCTTTC CTTCTGGGAA GTTCCTCATT GCTGGAGTCG TAGATGGTCG GAATATCTGG 
GCCAGTGATC TCGGAGCATC AATGTCCATT TTAAAAGCTC TAGAAGGAAT TGTTGGCAAA 
GAGAAGCTTG TCGTTTCCAC TTCCTGCTCT CTTTTGCACA CTGCTGTGGA CTTGGTAAAC 
GAAACAAAGC TCGATAGTGA GATAAAATCA TGGTTAGCAT TCGCAGCTCA AAAGATCCTT 
GAGGTGAGCA CTTTAGCGAA GGCTTTGGCT GGCAAAAAAG ATGAGGCATT CTTCATGGCA 
AATGCTGCTG CTATATCATC TAGAAAATCG TCTTCTAGGG TGAACAATGA AGCAGTTAAA 
ATATCGGCTT TGGCATTAAA GGGCTCTGAT CATCACCGAG CCACACCGGT GAGCACCCGA 
CTTGATGTAC AGCAAAAAGA GCTTAACCTG CCCATTCTAC CAACTACGAC AATTGGGTCT 
TTTCCACAAA CCATGGATCT CAGAAGGGTT CGCCGAGAAT ACAAGGCAAA CAATATCACT 
GAGGAAGAGT ACATGAGGGC CATTAAAGAG GAAATCTGTA AAGTTGTCAA GTTGCAAGAA 
GACCTTGGTA TTGACGTTCT TGTGCATGGA GAGCCAGAGA GGAACGATAT GGTGGAGTAT 
TTTGGTGAGC AATTGCATGG CTTTGCATTC ACTGTTAATG GCTGGGTTCA GTCCTATGGT 
TCCCGCTGCG TAAAACCCCC AATCATCTAT GGTGATGTAA GTCGCCCAAG GCCAATGACG 
ATTTTCTGGT CCAAAGCTGC ACAAAGCATG ACTTCTCGTC CTATGAAAGG AATGCTCACT 
GGACCAGTTA CTATTTTAAA CTGGTCATTT GTAAGAAATG ACCAACCTAG GTCAGAAACC 
TGTTACCAAA TTGCCTTGGC CATAAAAAAT GAGGTTGAAG ATTTAGAGGA CGCTGGCATT 
CAGGTGATCC AAATTGATGA AGCTGCCCTA CGAGAAGGTT TGCCTCTACG TAAGTCGGAG 
CAACCCTTTT ACCTGGACTG GGCTGTACAT GCTTTTAGAA TCACCAACTA TGGTGTGAAA 
GACACTACCC AGATTCACAC ACACATGTGC TACTCCAACT TCAACGACAT AATTCTCTCT 
ATCATGGACA TGGATGCTGA TGTGATTACA ATCGAGAATT CAAGATCCAA CGAGAAGCTC 
TTATCAGTGT TTAGAGAGGG CATCAAGTAT GGAGCAGGGA TTGGGCCTGG AGTGTATGAT 
ATACATTCAC CAAGAATCCC AACCACCGAG GAGATAGAGG AGAGGGTTAG AAAGATGTTG 
GGTGTGCTTG AGCCGAGTAT GGTATGGGTG AACCCAGACT GTGGTCTCAA GACCAGGAAG 
TACTCTGAGG TGATTCCTTC TCTAAGCAAC ATGGTTGCTG CTACAAAGCT TCTCCGAATT 
GAACTGGCCG ATACCAACAG GTGTTTTGAG TTGGGGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020521446.1
CDS	77..2398
Translation	MASHVVGYPRIGPKRELKFALESFWDGRSSAEDLQKVAKELRHSMWKQMSDVGIKYIPCNTFSYYDHVLDTTAMLGAVPPRYGWMGGEIGFDVYFSMARGNATLPAMEMTKWFDTNYHYLVPELGPDIKFSYSSHKAVSEYKEAKELGFETVPVLVGPVSYLLLSKPTKSVDRPFSLLSLLGKIIPIYKEVLAELKAAGAKWVQFDEPTLVLDLDSHQRKAFTEAYTELEESFSGLRVVVETYFADVPAEAYKTIIALKGISGIGFDLVRGEKTLDLIQNVGFPSGKFLIAGVVDGRNIWASDLGASMSILKALEGIVGKEKLVVSTSCSLLHTAVDLVNETKLDSEIKSWLAFAAQKILEVSTLAKALAGKKDEAFFMANAAAISSRKSSSRVNNEAVKISALALKGSDHHRATPVSTRLDVQQKELNLPILPTTTIGSFPQTMDLRRVRREYKANNITEEEYMRAIKEEICKVVKLQEDLGIDVLVHGEPERNDMVEYFGEQLHGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPRPMTIFWSKAAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKNEVEDLEDAGIQVIQIDEAALREGLPLRKSEQPFYLDWAVHAFRITNYGVKDTTQIHTHMCYSNFNDIILSIMDMDADVITIENSRSNEKLLSVFREGIKYGAGIGPGVYDIHSPRIPTTEEIEERVRKMLGVLEPSMVWVNPDCGLKTRKYSEVIPSLSNMVAATKLLRIELADTNRCFELGA

Target ORF information:

RefSeq Version	XM_020665787.1
Organism	Amborella trichopoda
Definition	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020665787.1

ORF Insert Sequence:

ATGGCTTCTC ATGTTGTTGG GTATCCGCGA ATTGGACCAA AGAGGGAGCT CAAGTTTGCT 
CTCGAGTCCT TTTGGGATGG CAGAAGTAGT GCTGAGGATT TGCAGAAGGT TGCAAAAGAG 
CTGAGGCATT CTATGTGGAA ACAAATGTCA GATGTGGGTA TAAAATATAT ACCCTGCAAT 
ACATTTTCAT ACTATGATCA TGTCCTAGAT ACAACTGCAA TGCTTGGTGC AGTTCCTCCA 
AGATATGGAT GGATGGGAGG TGAGATTGGT TTTGATGTTT ATTTCTCCAT GGCCAGAGGG 
AATGCTACTC TCCCTGCCAT GGAAATGACA AAATGGTTTG ATACCAACTA TCACTATCTT 
GTACCGGAAT TGGGACCTGA CATTAAATTC TCATATTCTT CTCACAAAGC TGTGTCTGAA 
TACAAGGAGG CAAAGGAGCT TGGATTTGAG ACTGTTCCAG TACTTGTGGG CCCCGTGTCT 
TACTTGTTGC TATCAAAACC CACAAAGAGT GTAGACAGAC CATTTTCTCT TCTTTCATTG 
TTGGGGAAGA TCATCCCTAT TTACAAGGAG GTTTTGGCTG AACTAAAGGC AGCTGGTGCG 
AAGTGGGTGC AGTTTGATGA GCCGACGCTC GTGTTAGATC TGGACTCTCA CCAACGAAAA 
GCATTTACTG AGGCCTATAC AGAGTTGGAA GAATCATTTT CTGGTTTGAG AGTGGTTGTC 
GAGACCTACT TTGCTGATGT GCCTGCTGAG GCATACAAGA CTATCATAGC ATTGAAGGGT 
ATCTCAGGTA TTGGTTTTGA TCTGGTGCGT GGAGAAAAGA CCCTTGATTT GATCCAAAAT 
GTAGGCTTTC CTTCTGGGAA GTTCCTCATT GCTGGAGTCG TAGATGGTCG GAATATCTGG 
GCCAGTGATC TCGGAGCATC AATGTCCATT TTAAAAGCTC TAGAAGGAAT TGTTGGCAAA 
GAGAAGCTTG TCGTTTCCAC TTCCTGCTCT CTTTTGCACA CTGCTGTGGA CTTGGTAAAC 
GAAACAAAGC TCGATAGTGA GATAAAATCA TGGTTAGCAT TCGCAGCTCA AAAGATCCTT 
GAGGTGAGCA CTTTAGCGAA GGCTTTGGCT GGCAAAAAAG ATGAGGCATT CTTCATGGCA 
AATGCTGCTG CTATATCATC TAGAAAATCG TCTTCTAGGG TGAACAATGA AGCAGTTAAA 
ATATCGGCTT TGGCATTAAA GGGCTCTGAT CATCACCGAG CCACACCGGT GAGCACCCGA 
CTTGATGTAC AGCAAAAAGA GCTTAACCTG CCCATTCTAC CAACTACGAC AATTGGGTCT 
TTTCCACAAA CCATGGATCT CAGAAGGGTT CGCCGAGAAT ACAAGGCAAA CAATATCACT 
GAGGAAGAGT ACATGAGGGC CATTAAAGAG GAAATCTGTA AAGTTGTCAA GTTGCAAGAA 
GACCTTGGTA TTGACGTTCT TGTGCATGGA GAGCCAGAGA GGAACGATAT GGTGGAGTAT 
TTTGGTGAGC AATTGCATGG CTTTGCATTC ACTGTTAATG GCTGGGTTCA GTCCTATGGT 
TCCCGCTGCG TAAAACCCCC AATCATCTAT GGTGATGTAA GTCGCCCAAG GCCAATGACG 
ATTTTCTGGT CCAAAGCTGC ACAAAGCATG ACTTCTCGTC CTATGAAAGG AATGCTCACT 
GGACCAGTTA CTATTTTAAA CTGGTCATTT GTAAGAAATG ACCAACCTAG GTCAGAAACC 
TGTTACCAAA TTGCCTTGGC CATAAAAAAT GAGGTTGAAG ATTTAGAGGA CGCTGGCATT 
CAGGTGATCC AAATTGATGA AGCTGCCCTA CGAGAAGGTT TGCCTCTACG TAAGTCGGAG 
CAACCCTTTT ACCTGGACTG GGCTGTACAT GCTTTTAGAA TCACCAACTA TGGTGTGAAA 
GACACTACCC AGATTCACAC ACACATGTGC TACTCCAACT TCAACGACAT AATTCTCTCT 
ATCATGGACA TGGATGCTGA TGTGATTACA ATCGAGAATT CAAGATCCAA CGAGAAGCTC 
TTATCAGTGT TTAGAGAGGG CATCAAGTAT GGAGCAGGGA TTGGGCCTGG AGTGTATGAT 
ATACATTCAC CAAGAATCCC AACCACCGAG GAGATAGAGG AGAGGGTTAG AAAGATGTTG 
GGTGTGCTTG AGCCGAGTAT GGTATGGGTG AACCCAGACT GTGGTCTCAA GACCAGGAAG 
TACTCTGAGG TGATTCCTTC TCTAAGCAAC ATGGTTGCTG CTACAAAGCT TCTCCGAATT 
GAACTGGCCG ATACCAACAG GTGTTTTGAG TTGGGGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18431955 ( XM_011624204.2 ) cDNA ORF clone, Amborella trichopoda -> XP_011622506.2 Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAm46257
Clone ID Related Accession (Same CDS sequence)	XM_011624204.2
Accession Version	XM_011624204.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-03
Organism	Amborella trichopoda
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006498085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 4, 2017 this sequence version replaced XM_011624204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amborella trichopoda Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACAT 
TCACACACAC ATGTGCTACT CCAACTTCAA CGACATAATT CTCTCTATCA TGGACATGGA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011622506.2
CDS	80..2125
Translation	MFPPMMSCLKPSFLSLKLRSSSLFSVHGYLRHRPVKIHSFIRSMASHVVGYPRIGPKRELKFALESFWDGRSSAEDLQKVAKELRHSMWKQMSDVGIKYIPCNTFSYYDHVLDTTAMLGAVPPRYGWMGGEIGFDVYFSMARGNATLPAMEMTKWFDTNYHYLVPELGPDIKFSYSSHKAVSEYKEAKELGFETVPVLVGPVSYLLLSKPTKSVDRPFSLLSLLGKIIPIYKEVLAELKAAGAKWVQFDEPTLVLDLDSHQRKAFTEAYTELEESFSGLRVVVETYFADVPAEAYKTIIALKGISGIGFDLVRGEKTLDLIQNVGFPSGKFLIAGVVDGRNIWASDLGASMSILKALEGIVGKEKLVVSTSCSLLHTAVDLVNETKLDSEIKSWLAFAAQKILEVSTLAKALAGKKDEAFFMANAAAISSRKSSSRVNNEAVKISALALKGSDHHRATPVSTRLDVQQKELNLPILPTTTIGSFPQTMDLRRVRREYKANNITEEEYMRAIKEEICKVVKLQEDLGIDVLVHGEPERNDMVEYFGEQLHGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPRPMTIFWSKAAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKNEVEDLEDAGIQVIQIDEAALREGLPLHSHTHVLLQLQRHNSLYHGHGC

Target ORF information:

RefSeq Version	XM_011624204.2
Organism	Amborella trichopoda
Definition	Amborella trichopoda 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC18431955), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011624204.2

ORF Insert Sequence:

ATGTTCCCGC CCATGATGTC ATGCTTAAAG CCCTCGTTTC TCTCTCTAAA ACTCCGTTCT 
TCTTCTCTCT TCTCCGTTCA TGGCTACCTA CGTCATCGCC CTGTAAAAAT CCACAGTTTC 
ATAAGGTCCA TGGCTTCTCA TGTTGTTGGG TATCCGCGAA TTGGACCAAA GAGGGAGCTC 
AAGTTTGCTC TCGAGTCCTT TTGGGATGGC AGAAGTAGTG CTGAGGATTT GCAGAAGGTT 
GCAAAAGAGC TGAGGCATTC TATGTGGAAA CAAATGTCAG ATGTGGGTAT AAAATATATA 
CCCTGCAATA CATTTTCATA CTATGATCAT GTCCTAGATA CAACTGCAAT GCTTGGTGCA 
GTTCCTCCAA GATATGGATG GATGGGAGGT GAGATTGGTT TTGATGTTTA TTTCTCCATG 
GCCAGAGGGA ATGCTACTCT CCCTGCCATG GAAATGACAA AATGGTTTGA TACCAACTAT 
CACTATCTTG TACCGGAATT GGGACCTGAC ATTAAATTCT CATATTCTTC TCACAAAGCT 
GTGTCTGAAT ACAAGGAGGC AAAGGAGCTT GGATTTGAGA CTGTTCCAGT ACTTGTGGGC 
CCCGTGTCTT ACTTGTTGCT ATCAAAACCC ACAAAGAGTG TAGACAGACC ATTTTCTCTT 
CTTTCATTGT TGGGGAAGAT CATCCCTATT TACAAGGAGG TTTTGGCTGA ACTAAAGGCA 
GCTGGTGCGA AGTGGGTGCA GTTTGATGAG CCGACGCTCG TGTTAGATCT GGACTCTCAC 
CAACGAAAAG CATTTACTGA GGCCTATACA GAGTTGGAAG AATCATTTTC TGGTTTGAGA 
GTGGTTGTCG AGACCTACTT TGCTGATGTG CCTGCTGAGG CATACAAGAC TATCATAGCA 
TTGAAGGGTA TCTCAGGTAT TGGTTTTGAT CTGGTGCGTG GAGAAAAGAC CCTTGATTTG 
ATCCAAAATG TAGGCTTTCC TTCTGGGAAG TTCCTCATTG CTGGAGTCGT AGATGGTCGG 
AATATCTGGG CCAGTGATCT CGGAGCATCA ATGTCCATTT TAAAAGCTCT AGAAGGAATT 
GTTGGCAAAG AGAAGCTTGT CGTTTCCACT TCCTGCTCTC TTTTGCACAC TGCTGTGGAC 
TTGGTAAACG AAACAAAGCT CGATAGTGAG ATAAAATCAT GGTTAGCATT CGCAGCTCAA 
AAGATCCTTG AGGTGAGCAC TTTAGCGAAG GCTTTGGCTG GCAAAAAAGA TGAGGCATTC 
TTCATGGCAA ATGCTGCTGC TATATCATCT AGAAAATCGT CTTCTAGGGT GAACAATGAA 
GCAGTTAAAA TATCGGCTTT GGCATTAAAG GGCTCTGATC ATCACCGAGC CACACCGGTG 
AGCACCCGAC TTGATGTACA GCAAAAAGAG CTTAACCTGC CCATTCTACC AACTACGACA 
ATTGGGTCTT TTCCACAAAC CATGGATCTC AGAAGGGTTC GCCGAGAATA CAAGGCAAAC 
AATATCACTG AGGAAGAGTA CATGAGGGCC ATTAAAGAGG AAATCTGTAA AGTTGTCAAG 
TTGCAAGAAG ACCTTGGTAT TGACGTTCTT GTGCATGGAG AGCCAGAGAG GAACGATATG 
GTGGAGTATT TTGGTGAGCA ATTGCATGGC TTTGCATTCA CTGTTAATGG CTGGGTTCAG 
TCCTATGGTT CCCGCTGCGT AAAACCCCCA ATCATCTATG GTGATGTAAG TCGCCCAAGG 
CCAATGACGA TTTTCTGGTC CAAAGCTGCA CAAAGCATGA CTTCTCGTCC TATGAAAGGA 
ATGCTCACTG GACCAGTTAC TATTTTAAAC TGGTCATTTG TAAGAAATGA CCAACCTAGG 
TCAGAAACCT GTTACCAAAT TGCCTTGGCC ATAAAAAATG AGGTTGAAGA TTTAGAGGAC 
GCTGGCATTC AGGTGATCCA AATTGATGAA GCTGCCCTAC GAGAAGGTTT GCCTCTACAT 
TCACACACAC ATGTGCTACT CCAACTTCAA CGACATAATT CTCTCTATCA TGGACATGGA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC18431955 cDNA ORF clone, Amborella trichopoda

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム