NEDD4L gene cDNA ORF clone, Alligator sinensis(Chinese alligator)

mRNA	Protein	Name
XM_006019094.2	XP_006019156.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_006019095.2	XP_006019157.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_025198462.1	XP_025054247.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_025198463.1	XP_025054248.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_025198469.1	XP_025054254.1	E3 ubiquitin-protein ligase NEDD4-like isoform X8
XM_025198470.1	XP_025054255.1	E3 ubiquitin-protein ligase NEDD4-like isoform X9
XM_025198471.1	XP_025054256.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_006019097.2	XP_006019159.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_025198464.1	XP_025054249.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_025198468.1	XP_025054253.1	E3 ubiquitin-protein ligase NEDD4-like isoform X7
XM_006019097.3	XP_006019159.1	E3 ubiquitin-protein ligase NEDD4-like isoform X12
XM_006019096.2	XP_006019158.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_006019096.3	XP_006019158.1	E3 ubiquitin-protein ligase NEDD4-like isoform X11
XM_025198465.1	XP_025054250.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_025198466.1	XP_025054251.1	E3 ubiquitin-protein ligase NEDD4-like isoform X5
XM_025198467.1	XP_025054252.1	E3 ubiquitin-protein ligase NEDD4-like isoform X6
XM_025198472.1	XP_025054257.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_025198473.1	XP_025054258.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_025198474.1	XP_025054259.1	E3 ubiquitin-protein ligase NEDD4-like isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl13941	XM_006019094.2 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl13942	XM_006019095.2 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl13943	XM_006019096.3 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl13944	XM_006019097.3 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl83312	XM_025198470.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAl83313	XM_025198471.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAl83313	XM_025198473.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAl83313	XM_025198472.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAl83314	XM_025198474.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAl83304	XM_025198462.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83305	XM_025198463.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83306	XM_025198464.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83307	XM_025198465.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83308	XM_025198466.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83309	XM_025198467.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83310	XM_025198468.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl83311	XM_025198469.1 Latest version!	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OAl13944	XM_006019097.2	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl13943	XM_006019096.2	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

NEDD4L ( XM_006019094.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019156.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl13941
Clone ID Related Accession (Same CDS sequence)	XM_006019094.2
Accession Version	XM_006019094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006019094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTG AAAGAAAAGG AGGTACTTGG AACTGTGACC CGTATGTGAA ACTTTCCCTA 
TATGTAGCAG ATGAAAACAG AGAACTTGCT TTGGTGCAGA CAAAAACTAT TAAAAAGACA 
CTGAATCCCA AATGGAATGA AGAATTTTAT TTTAGAGTAA ACCCAACCAA TCATCGACTC 
CTGTTTGAAG TGTTTGATGA AAACAGATTG ACTAGAGACG ACTTCCTTGG TCAAGTAGAC 
GTCCCACTTA ATCATCTTCC GACAGAAGAC CCAACCATGG AACGGCCATA CACGTTTAAG 
GATTTTCTTC TCAGACCAAG AAGCCACAAA TCTCGGGTAA AGGGTTTCTT GAGACTGAAG 
ATGGCCTATA TGCCGAAAAA TGGAGGACAA GAGGATGAAA ATAATGATCA AAGAGATGAC 
TCGGAGCATG GGTGGGAAGT GGTTGATTCC AATGATTCTG CATCTCATCG CCAAGAGGAG 
TTACCGCCAC CTCCACTGCC TCCTGGATGG GAAGAAAAAG TGGACAATCT TGGTCGTACT 
TACTATGTCA ACCACAACAA CAGGACAACT CAGTGGCACC GGCCAACTTT AATTGATATG 
GCATCTGAAT CAGACAACAA CATTAGACAA ATAAACCAAG AGGCGGCTCA TAGACGCTTT 
CGATCTCGGA GACACATTAG TGAAGATTTG GAGCCAGAGC CCATGGACAG TGGAGATATA 
CCTGAACCCT GGGAAACTAT TTCAGAGGAA GTGAGTGCAA GTGGAGATTC CTTAAGCCTG 
TCCTTGCCAC CTCCCCCGGC CTCTCCAGTT TCTCGGACCA GTCCCCAGGA GCTGTCTGAT 
GAATTGAACA GAAGGCTCCA AGTCACTCCT GATTCCAATG GGGAACAACT CAGTTCTTTG 
ATACAAAGAG ATCCTTCATC AAGATTAAGG TCTTGCAGCG TAACAGATGC AGTTGCTGAA 
CAGTCTCAAT TATCACTGCA GAGTGGTCCA TCTAGGAGAG CTCGTTCTTC AACTGTCACA 
GGTGGTGAAG AACCAACGCC ATCAGTGGCC TATGTGCATA CAACACCGGG CTTACCTTCA 
GGCTGGGAAG AAAGGAAAGA TGCTAAGGGA CGTACTTATT ATGTCAATCA TAACAATCGA 
ACCACAACTT GGACTCGGCC CATTATGCAG CTTGCAGAAG ATGGTGCAGT TGGGTCAGCA 
ACAAACAGTA ACAACCATCT CAGTGAACCT CAGATAAGAC GGCCTCGTAG CCTCAGTTCA 
CCCACAGTAA CTTTATCTGC TCCACTTGAG GGACTCTCCA AGGACTCTCC TGCACGCCGA 
GCTGTAAAGG ATACCCTTTC GAATCCTCAG TCTCCCCAGC CCTCACCTTA TAATTCTCCT 
AAACCACAAC ACAAAGTGAC ACAGAGCTTC TTGCCCCCTG GCTGGGAGAT GCGAATAGCT 
CCAAATGGCA GGCCCTTCTT CATTGATCAC AATACTAAAA CCACTACCTG GGAAGATCCA 
CGACTGAAAT TTCCAGTACA TTTGAGATCA AAAGCTTCTT TGAACCCTAA TGATTTGGGC 
CCTCTTCCTC CTGGTTGGGA GGAAAGAATA CATTTGGATG GAAGAACGTT TTATATAGAC 
CATAATAATA AAATTACCCA GTGGGAAGAT CCCAGATTGC AGAACCCAGC TATTACTGGT 
CCAGCTGTTC CCTATTCCAG AGAGTTCAAA CAGAAATATG ACTACTTCAG AAAGAAATTA 
AAGAAACCAG CTGATCTACC AAACAGATTT GAGATGAAAC TCCACAGAAA TAATATTTTT 
GAGGAGTCCT ATAGAAGAAT CATGTCCGTG AAGAGACCCG ATGTATTAAA AGCCAGGCTC 
TGGATTGAAT TTGAAGCGGA AAAAGGACTT GATTATGGAG GTGTGGCCAG AGAATGGTTC 
TTTCTGTTGT CCAAGGAAAT GTTTAATCCT TATTATGGTC TTTTTGAATA TTCGGCAACG 
GACAACTATA CTCTTCAGAT TAATCCAAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC 
TATTTCACAT TTATTGGCCG GGTTGCTGGG CTGGCAGTGT ATCATGGGAA GTTATTAGAT 
GGCTTTTTCA TCAGGCCTTT TTATAAGATG ATGCTGGGGA AACCAATAAC TCTGAAAGAC 
ATGGAATCAG TGGATAGTGA ATATTACAAT TCCTTGAAAT GGATCCTGGA AAATGACCCA 
ACAGAGCTGG ATCTCATGTT TTGCATAGAT GAGGAAAACT TTGGACAGAC TTATCAAGTG 
GACTTGAAGC CTAATGGATC AGAAATCATG GTAACAAATG AGAACAAACG GGAATATATA 
GACTTAGTCA TCCAATGGAG GTTTGTAAAC AGAGTTCAGA AACAAATGAA TGCTTTTTTG 
GAGGGATTCA CAGAACTCCT ACCTACTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG 
GAGTTACTAA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATACGATC 
TACAAGAACG GATACTGTCC GAACCATCCT TTAATCCAGT GGTTCTGGAA GGCTGTGTTA 
CTGATGGATG CTGAGAAACG TATCCGGTTA CTGCAGTTTG TTACGGGGAC ATCTCGAGTG 
CCTATGAATG GATTTGCTGA ACTTTACGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGATAA ATTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA 
CCTCCTTATG AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT 
CAAGGATTTG AAGGAGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019156.1
CDS	37..2880
Translation	MNVERKGGTWNCDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPTNHRLLFEVFDENRLTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019094.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019094.2

ORF Insert Sequence:

ATGAATGTTG AAAGAAAAGG AGGTACTTGG AACTGTGACC CGTATGTGAA ACTTTCCCTA 
TATGTAGCAG ATGAAAACAG AGAACTTGCT TTGGTGCAGA CAAAAACTAT TAAAAAGACA 
CTGAATCCCA AATGGAATGA AGAATTTTAT TTTAGAGTAA ACCCAACCAA TCATCGACTC 
CTGTTTGAAG TGTTTGATGA AAACAGATTG ACTAGAGACG ACTTCCTTGG TCAAGTAGAC 
GTCCCACTTA ATCATCTTCC GACAGAAGAC CCAACCATGG AACGGCCATA CACGTTTAAG 
GATTTTCTTC TCAGACCAAG AAGCCACAAA TCTCGGGTAA AGGGTTTCTT GAGACTGAAG 
ATGGCCTATA TGCCGAAAAA TGGAGGACAA GAGGATGAAA ATAATGATCA AAGAGATGAC 
TCGGAGCATG GGTGGGAAGT GGTTGATTCC AATGATTCTG CATCTCATCG CCAAGAGGAG 
TTACCGCCAC CTCCACTGCC TCCTGGATGG GAAGAAAAAG TGGACAATCT TGGTCGTACT 
TACTATGTCA ACCACAACAA CAGGACAACT CAGTGGCACC GGCCAACTTT AATTGATATG 
GCATCTGAAT CAGACAACAA CATTAGACAA ATAAACCAAG AGGCGGCTCA TAGACGCTTT 
CGATCTCGGA GACACATTAG TGAAGATTTG GAGCCAGAGC CCATGGACAG TGGAGATATA 
CCTGAACCCT GGGAAACTAT TTCAGAGGAA GTGAGTGCAA GTGGAGATTC CTTAAGCCTG 
TCCTTGCCAC CTCCCCCGGC CTCTCCAGTT TCTCGGACCA GTCCCCAGGA GCTGTCTGAT 
GAATTGAACA GAAGGCTCCA AGTCACTCCT GATTCCAATG GGGAACAACT CAGTTCTTTG 
ATACAAAGAG ATCCTTCATC AAGATTAAGG TCTTGCAGCG TAACAGATGC AGTTGCTGAA 
CAGTCTCAAT TATCACTGCA GAGTGGTCCA TCTAGGAGAG CTCGTTCTTC AACTGTCACA 
GGTGGTGAAG AACCAACGCC ATCAGTGGCC TATGTGCATA CAACACCGGG CTTACCTTCA 
GGCTGGGAAG AAAGGAAAGA TGCTAAGGGA CGTACTTATT ATGTCAATCA TAACAATCGA 
ACCACAACTT GGACTCGGCC CATTATGCAG CTTGCAGAAG ATGGTGCAGT TGGGTCAGCA 
ACAAACAGTA ACAACCATCT CAGTGAACCT CAGATAAGAC GGCCTCGTAG CCTCAGTTCA 
CCCACAGTAA CTTTATCTGC TCCACTTGAG GGACTCTCCA AGGACTCTCC TGCACGCCGA 
GCTGTAAAGG ATACCCTTTC GAATCCTCAG TCTCCCCAGC CCTCACCTTA TAATTCTCCT 
AAACCACAAC ACAAAGTGAC ACAGAGCTTC TTGCCCCCTG GCTGGGAGAT GCGAATAGCT 
CCAAATGGCA GGCCCTTCTT CATTGATCAC AATACTAAAA CCACTACCTG GGAAGATCCA 
CGACTGAAAT TTCCAGTACA TTTGAGATCA AAAGCTTCTT TGAACCCTAA TGATTTGGGC 
CCTCTTCCTC CTGGTTGGGA GGAAAGAATA CATTTGGATG GAAGAACGTT TTATATAGAC 
CATAATAATA AAATTACCCA GTGGGAAGAT CCCAGATTGC AGAACCCAGC TATTACTGGT 
CCAGCTGTTC CCTATTCCAG AGAGTTCAAA CAGAAATATG ACTACTTCAG AAAGAAATTA 
AAGAAACCAG CTGATCTACC AAACAGATTT GAGATGAAAC TCCACAGAAA TAATATTTTT 
GAGGAGTCCT ATAGAAGAAT CATGTCCGTG AAGAGACCCG ATGTATTAAA AGCCAGGCTC 
TGGATTGAAT TTGAAGCGGA AAAAGGACTT GATTATGGAG GTGTGGCCAG AGAATGGTTC 
TTTCTGTTGT CCAAGGAAAT GTTTAATCCT TATTATGGTC TTTTTGAATA TTCGGCAACG 
GACAACTATA CTCTTCAGAT TAATCCAAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC 
TATTTCACAT TTATTGGCCG GGTTGCTGGG CTGGCAGTGT ATCATGGGAA GTTATTAGAT 
GGCTTTTTCA TCAGGCCTTT TTATAAGATG ATGCTGGGGA AACCAATAAC TCTGAAAGAC 
ATGGAATCAG TGGATAGTGA ATATTACAAT TCCTTGAAAT GGATCCTGGA AAATGACCCA 
ACAGAGCTGG ATCTCATGTT TTGCATAGAT GAGGAAAACT TTGGACAGAC TTATCAAGTG 
GACTTGAAGC CTAATGGATC AGAAATCATG GTAACAAATG AGAACAAACG GGAATATATA 
GACTTAGTCA TCCAATGGAG GTTTGTAAAC AGAGTTCAGA AACAAATGAA TGCTTTTTTG 
GAGGGATTCA CAGAACTCCT ACCTACTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG 
GAGTTACTAA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATACGATC 
TACAAGAACG GATACTGTCC GAACCATCCT TTAATCCAGT GGTTCTGGAA GGCTGTGTTA 
CTGATGGATG CTGAGAAACG TATCCGGTTA CTGCAGTTTG TTACGGGGAC ATCTCGAGTG 
CCTATGAATG GATTTGCTGA ACTTTACGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGATAA ATTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA 
CCTCCTTATG AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT 
CAAGGATTTG AAGGAGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006019095.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019157.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAl13942
Clone ID Related Accession (Same CDS sequence)	XM_006019095.2
Accession Version	XM_006019095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006019095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTTG AAAGAAAAGG AGGTACTTGG AACTGTGACC CGTATGTGAA ACTTTCCCTA 
TATGTAGCAG ATGAAAACAG AGAACTTGCT TTGGTGCAGA CAAAAACTAT TAAAAAGACA 
CTGAATCCCA AATGGAATGA AGAATTTTAT TTTAGAGTAA ACCCAACCAA TCATCGACTC 
CTGTTTGAAG TGTTTGATGA AAACAGATTG ACTAGAGACG ACTTCCTTGG TCAAGTAGAC 
GTCCCACTTA ATCATCTTCC GACAGAAGAC CCAACCATGG AACGGCCATA CACGTTTAAG 
GATTTTCTTC TCAGACCAAG AAGCCACAAA TCTCGGGTAA AGGGTTTCTT GAGACTGAAG 
ATGGCCTATA TGCCGAAAAA TGGAGGACAA GAGGATGAAA ATAATGATCA AAGAGATGAC 
TCGGAGCATG GGTGGGAAGT GGTTGATTCC AATGATTCTG CATCTCATCG CCAAGAGGAG 
TTACCGCCAC CTCCACTGCC TCCTGGATGG GAAGAAAAAG TGGACAATCT TGGTCGTACT 
TACTATGTCA ACCACAACAA CAGGACAACT CAGTGGCACC GGCCAACTTT AATTGATATG 
GCATCTGAAT CAGACAACAA CATTAGACAA ATAAACCAAG AGGCGGCTCA TAGACGCTTT 
CGATCTCGGA GACACATTAG TGAAGATTTG GAGCCAGAGC CCATGGACAG TGGAGATATA 
CCTGAACCCT GGGAAACTAT TTCAGAGGAA GTGAGTGCAA GTGGAGATTC CTTAAGCCTG 
TCCTTGCCAC CTCCCCCGGC CTCTCCAGTT TCTCGGACCA GTCCCCAGGA GCTGTCTGAT 
GAATTGAACA GAAGGCTCCA AGTCACTCCT GATTCCAATG GGGAACAACT CAGTTCTTTG 
ATACAAAGAG ATCCTTCATC AAGATTAAGG TCTTGCAGCG TAACAGATGC AGTTGCTGAA 
CAGTCTCAAT TATCACTGCC ATCAGTGGCC TATGTGCATA CAACACCGGG CTTACCTTCA 
GGCTGGGAAG AAAGGAAAGA TGCTAAGGGA CGTACTTATT ATGTCAATCA TAACAATCGA 
ACCACAACTT GGACTCGGCC CATTATGCAG CTTGCAGAAG ATGGTGCAGT TGGGTCAGCA 
ACAAACAGTA ACAACCATCT CAGTGAACCT CAGATAAGAC GGCCTCGTAG CCTCAGTTCA 
CCCACAGTAA CTTTATCTGC TCCACTTGAG GGACTCTCCA AGGACTCTCC TGCACGCCGA 
GCTGTAAAGG ATACCCTTTC GAATCCTCAG TCTCCCCAGC CCTCACCTTA TAATTCTCCT 
AAACCACAAC ACAAAGTGAC ACAGAGCTTC TTGCCCCCTG GCTGGGAGAT GCGAATAGCT 
CCAAATGGCA GGCCCTTCTT CATTGATCAC AATACTAAAA CCACTACCTG GGAAGATCCA 
CGACTGAAAT TTCCAGTACA TTTGAGATCA AAAGCTTCTT TGAACCCTAA TGATTTGGGC 
CCTCTTCCTC CTGGTTGGGA GGAAAGAATA CATTTGGATG GAAGAACGTT TTATATAGAC 
CATAATAATA AAATTACCCA GTGGGAAGAT CCCAGATTGC AGAACCCAGC TATTACTGGT 
CCAGCTGTTC CCTATTCCAG AGAGTTCAAA CAGAAATATG ACTACTTCAG AAAGAAATTA 
AAGAAACCAG CTGATCTACC AAACAGATTT GAGATGAAAC TCCACAGAAA TAATATTTTT 
GAGGAGTCCT ATAGAAGAAT CATGTCCGTG AAGAGACCCG ATGTATTAAA AGCCAGGCTC 
TGGATTGAAT TTGAAGCGGA AAAAGGACTT GATTATGGAG GTGTGGCCAG AGAATGGTTC 
TTTCTGTTGT CCAAGGAAAT GTTTAATCCT TATTATGGTC TTTTTGAATA TTCGGCAACG 
GACAACTATA CTCTTCAGAT TAATCCAAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC 
TATTTCACAT TTATTGGCCG GGTTGCTGGG CTGGCAGTGT ATCATGGGAA GTTATTAGAT 
GGCTTTTTCA TCAGGCCTTT TTATAAGATG ATGCTGGGGA AACCAATAAC TCTGAAAGAC 
ATGGAATCAG TGGATAGTGA ATATTACAAT TCCTTGAAAT GGATCCTGGA AAATGACCCA 
ACAGAGCTGG ATCTCATGTT TTGCATAGAT GAGGAAAACT TTGGACAGAC TTATCAAGTG 
GACTTGAAGC CTAATGGATC AGAAATCATG GTAACAAATG AGAACAAACG GGAATATATA 
GACTTAGTCA TCCAATGGAG GTTTGTAAAC AGAGTTCAGA AACAAATGAA TGCTTTTTTG 
GAGGGATTCA CAGAACTCCT ACCTACTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG 
GAGTTACTAA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATACGATC 
TACAAGAACG GATACTGTCC GAACCATCCT TTAATCCAGT GGTTCTGGAA GGCTGTGTTA 
CTGATGGATG CTGAGAAACG TATCCGGTTA CTGCAGTTTG TTACGGGGAC ATCTCGAGTG 
CCTATGAATG GATTTGCTGA ACTTTACGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGATAA ATTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA 
CCTCCTTATG AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT 
CAAGGATTTG AAGGAGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019157.1
CDS	37..2820
Translation	MNVERKGGTWNCDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPTNHRLLFEVFDENRLTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019095.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019095.2

ORF Insert Sequence:

ATGAATGTTG AAAGAAAAGG AGGTACTTGG AACTGTGACC CGTATGTGAA ACTTTCCCTA 
TATGTAGCAG ATGAAAACAG AGAACTTGCT TTGGTGCAGA CAAAAACTAT TAAAAAGACA 
CTGAATCCCA AATGGAATGA AGAATTTTAT TTTAGAGTAA ACCCAACCAA TCATCGACTC 
CTGTTTGAAG TGTTTGATGA AAACAGATTG ACTAGAGACG ACTTCCTTGG TCAAGTAGAC 
GTCCCACTTA ATCATCTTCC GACAGAAGAC CCAACCATGG AACGGCCATA CACGTTTAAG 
GATTTTCTTC TCAGACCAAG AAGCCACAAA TCTCGGGTAA AGGGTTTCTT GAGACTGAAG 
ATGGCCTATA TGCCGAAAAA TGGAGGACAA GAGGATGAAA ATAATGATCA AAGAGATGAC 
TCGGAGCATG GGTGGGAAGT GGTTGATTCC AATGATTCTG CATCTCATCG CCAAGAGGAG 
TTACCGCCAC CTCCACTGCC TCCTGGATGG GAAGAAAAAG TGGACAATCT TGGTCGTACT 
TACTATGTCA ACCACAACAA CAGGACAACT CAGTGGCACC GGCCAACTTT AATTGATATG 
GCATCTGAAT CAGACAACAA CATTAGACAA ATAAACCAAG AGGCGGCTCA TAGACGCTTT 
CGATCTCGGA GACACATTAG TGAAGATTTG GAGCCAGAGC CCATGGACAG TGGAGATATA 
CCTGAACCCT GGGAAACTAT TTCAGAGGAA GTGAGTGCAA GTGGAGATTC CTTAAGCCTG 
TCCTTGCCAC CTCCCCCGGC CTCTCCAGTT TCTCGGACCA GTCCCCAGGA GCTGTCTGAT 
GAATTGAACA GAAGGCTCCA AGTCACTCCT GATTCCAATG GGGAACAACT CAGTTCTTTG 
ATACAAAGAG ATCCTTCATC AAGATTAAGG TCTTGCAGCG TAACAGATGC AGTTGCTGAA 
CAGTCTCAAT TATCACTGCC ATCAGTGGCC TATGTGCATA CAACACCGGG CTTACCTTCA 
GGCTGGGAAG AAAGGAAAGA TGCTAAGGGA CGTACTTATT ATGTCAATCA TAACAATCGA 
ACCACAACTT GGACTCGGCC CATTATGCAG CTTGCAGAAG ATGGTGCAGT TGGGTCAGCA 
ACAAACAGTA ACAACCATCT CAGTGAACCT CAGATAAGAC GGCCTCGTAG CCTCAGTTCA 
CCCACAGTAA CTTTATCTGC TCCACTTGAG GGACTCTCCA AGGACTCTCC TGCACGCCGA 
GCTGTAAAGG ATACCCTTTC GAATCCTCAG TCTCCCCAGC CCTCACCTTA TAATTCTCCT 
AAACCACAAC ACAAAGTGAC ACAGAGCTTC TTGCCCCCTG GCTGGGAGAT GCGAATAGCT 
CCAAATGGCA GGCCCTTCTT CATTGATCAC AATACTAAAA CCACTACCTG GGAAGATCCA 
CGACTGAAAT TTCCAGTACA TTTGAGATCA AAAGCTTCTT TGAACCCTAA TGATTTGGGC 
CCTCTTCCTC CTGGTTGGGA GGAAAGAATA CATTTGGATG GAAGAACGTT TTATATAGAC 
CATAATAATA AAATTACCCA GTGGGAAGAT CCCAGATTGC AGAACCCAGC TATTACTGGT 
CCAGCTGTTC CCTATTCCAG AGAGTTCAAA CAGAAATATG ACTACTTCAG AAAGAAATTA 
AAGAAACCAG CTGATCTACC AAACAGATTT GAGATGAAAC TCCACAGAAA TAATATTTTT 
GAGGAGTCCT ATAGAAGAAT CATGTCCGTG AAGAGACCCG ATGTATTAAA AGCCAGGCTC 
TGGATTGAAT TTGAAGCGGA AAAAGGACTT GATTATGGAG GTGTGGCCAG AGAATGGTTC 
TTTCTGTTGT CCAAGGAAAT GTTTAATCCT TATTATGGTC TTTTTGAATA TTCGGCAACG 
GACAACTATA CTCTTCAGAT TAATCCAAAT TCAGGCCTTT GTAATGAAGA TCATCTGTCC 
TATTTCACAT TTATTGGCCG GGTTGCTGGG CTGGCAGTGT ATCATGGGAA GTTATTAGAT 
GGCTTTTTCA TCAGGCCTTT TTATAAGATG ATGCTGGGGA AACCAATAAC TCTGAAAGAC 
ATGGAATCAG TGGATAGTGA ATATTACAAT TCCTTGAAAT GGATCCTGGA AAATGACCCA 
ACAGAGCTGG ATCTCATGTT TTGCATAGAT GAGGAAAACT TTGGACAGAC TTATCAAGTG 
GACTTGAAGC CTAATGGATC AGAAATCATG GTAACAAATG AGAACAAACG GGAATATATA 
GACTTAGTCA TCCAATGGAG GTTTGTAAAC AGAGTTCAGA AACAAATGAA TGCTTTTTTG 
GAGGGATTCA CAGAACTCCT ACCTACTGAT TTGATTAAAA TTTTTGATGA AAATGAGCTG 
GAGTTACTAA TGTGTGGCCT TGGAGATGTT GATGTTAATG ACTGGAGACA ACATACGATC 
TACAAGAACG GATACTGTCC GAACCATCCT TTAATCCAGT GGTTCTGGAA GGCTGTGTTA 
CTGATGGATG CTGAGAAACG TATCCGGTTA CTGCAGTTTG TTACGGGGAC ATCTCGAGTG 
CCTATGAATG GATTTGCTGA ACTTTACGGT TCAAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGATAA ATTGCCCAGA GCTCACACAT GCTTTAATCG CCTTGACTTA 
CCTCCTTATG AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT 
CAAGGATTTG AAGGAGTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006019096.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019158.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAl13943
Clone ID Related Accession (Same CDS sequence)	XM_006019096.2 , XM_006019096.3
Accession Version	XM_006019096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006019096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019158.1
CDS	313..2880
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019096.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019096.2

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006019096.3 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019158.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OAl13943
Clone ID Related Accession (Same CDS sequence)	XM_006019096.2 , XM_006019096.3
Accession Version	XM_006019096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006019096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019158.1
CDS	448..3015
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019096.3
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019096.3

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006019097.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019159.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAl13944
Clone ID Related Accession (Same CDS sequence)	XM_006019097.2 , XM_006019097.3
Accession Version	XM_006019097.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006019097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019159.1
CDS	312..2819
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019097.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019097.2

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006019097.3 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006019159.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OAl13944
Clone ID Related Accession (Same CDS sequence)	XM_006019097.2 , XM_006019097.3
Accession Version	XM_006019097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006019097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006019159.1
CDS	448..2955
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006019097.3
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006019097.3

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAAT AATAAAATTA CCCAGTGGGA AGATCCCAGA 
TTGCAGAACC CAGCTATTAC TGGTCCAGCT GTTCCCTATT CCAGAGAGTT CAAACAGAAA 
TATGACTACT TCAGAAAGAA ATTAAAGAAA CCAGCTGATC TACCAAACAG ATTTGAGATG 
AAACTCCACA GAAATAATAT TTTTGAGGAG TCCTATAGAA GAATCATGTC CGTGAAGAGA 
CCCGATGTAT TAAAAGCCAG GCTCTGGATT GAATTTGAAG CGGAAAAAGG ACTTGATTAT 
GGAGGTGTGG CCAGAGAATG GTTCTTTCTG TTGTCCAAGG AAATGTTTAA TCCTTATTAT 
GGTCTTTTTG AATATTCGGC AACGGACAAC TATACTCTTC AGATTAATCC AAATTCAGGC 
CTTTGTAATG AAGATCATCT GTCCTATTTC ACATTTATTG GCCGGGTTGC TGGGCTGGCA 
GTGTATCATG GGAAGTTATT AGATGGCTTT TTCATCAGGC CTTTTTATAA GATGATGCTG 
GGGAAACCAA TAACTCTGAA AGACATGGAA TCAGTGGATA GTGAATATTA CAATTCCTTG 
AAATGGATCC TGGAAAATGA CCCAACAGAG CTGGATCTCA TGTTTTGCAT AGATGAGGAA 
AACTTTGGAC AGACTTATCA AGTGGACTTG AAGCCTAATG GATCAGAAAT CATGGTAACA 
AATGAGAACA AACGGGAATA TATAGACTTA GTCATCCAAT GGAGGTTTGT AAACAGAGTT 
CAGAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TCCTACCTAC TGATTTGATT 
AAAATTTTTG ATGAAAATGA GCTGGAGTTA CTAATGTGTG GCCTTGGAGA TGTTGATGTT 
AATGACTGGA GACAACATAC GATCTACAAG AACGGATACT GTCCGAACCA TCCTTTAATC 
CAGTGGTTCT GGAAGGCTGT GTTACTGATG GATGCTGAGA AACGTATCCG GTTACTGCAG 
TTTGTTACGG GGACATCTCG AGTGCCTATG AATGGATTTG CTGAACTTTA CGGTTCAAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG ATAAATTGCC CAGAGCTCAC 
ACATGCTTTA ATCGCCTTGA CTTACCTCCT TATGAATCTT TTGAAGATTT ACGAGAGAAA 
CTTCTTATGG CAGTGGAAAA TGCTCAAGGA TTTGAAGGAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198470.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054255.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OAl83312
Clone ID Related Accession (Same CDS sequence)	XM_025198470.1
Accession Version	XM_025198470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTA GAGACGACTT CCTTGGTCAA GTAGACGTCC CACTTAATCA TCTTCCGACA 
GAAGACCCAA CCATGGAACG GCCATACACG TTTAAGGATT TTCTTCTCAG ACCAAGAAGC 
CACAAATCTC GGGTAAAGGG TTTCTTGAGA CTGAAGATGG CCTATATGCC GAAAAATGGA 
GGACAAGAGG ATGAAAATAA TGATCAAAGA GATGACTCGG AGCATGGGTG GGAAGTGGTT 
GATTCCAATG ATTCTGCATC TCATCGCCAA GAGGAGTTAC CGCCACCTCC ACTGCCTCCT 
GGATGGGAAG AAAAAGTGGA CAATCTTGGT CGTACTTACT ATGTCAACCA CAACAACAGG 
ACAACTCAGT GGCACCGGCC AACTTTAATT GATATGGCAT CTGAATCAGA CAACAACATT 
AGACAAATAA ACCAAGAGGC GGCTCATAGA CGCTTTCGAT CTCGGAGACA CATTAGTGAA 
GATTTGGAGC CAGAGCCCAT GGACAGTGGA GATATACCTG AACCCTGGGA AACTATTTCA 
GAGGAAGTGA GTGCAAGTGG AGATTCCTTA AGCCTGTCCT TGCCACCTCC CCCGGCCTCT 
CCAGTTTCTC GGACCAGTCC CCAGGAGCTG TCTGATGAAT TGAACAGAAG GCTCCAAGTC 
ACTCCTGATT CCAATGGGGA ACAACTCAGT TCTTTGATAC AAAGAGATCC TTCATCAAGA 
TTAAGGTCTT GCAGCGTAAC AGATGCAGTT GCTGAACAGT CTCAATTATC ACTGCAGAGT 
GGTCCATCTA GGAGAGCTCG TTCTTCAACT GTCACAGGTG GTGAAGAACC AACGCCATCA 
GTGGCCTATG TGCATACAAC ACCGGGCTTA CCTTCAGGCT GGGAAGAAAG GAAAGATGCT 
AAGGGACGTA CTTATTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCCATT 
ATGCAGCTTG CAGAAGATGG TGCAGTTGGG TCAGCAACAA ACAGTAACAA CCATCTCAGT 
GAACCTCAGA TAAGACGGCC TCGTAGCCTC AGTTCACCCA CAGTAACTTT ATCTGCTCCA 
CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA CGCCGAGCTG TAAAGGATAC CCTTTCGAAT 
CCTCAGTCTC CCCAGCCCTC ACCTTATAAT TCTCCTAAAC CACAACACAA AGTGACACAG 
AGCTTCTTGC CCCCTGGCTG GGAGATGCGA ATAGCTCCAA ATGGCAGGCC CTTCTTCATT 
GATCACAATA CTAAAACCAC TACCTGGGAA GATCCACGAC TGAAATTTCC AGTACATTTG 
AGATCAAAAG CTTCTTTGAA CCCTAATGAT TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA 
AGAATACATT TGGATGGAAG AACGTTTTAT ATAGACCATA GAAGCCAGGT GTTGATATGT 
GGAGACATTG ATACCAGAGA CTTAGTGCCC TCATACGATA ATAAAATTAC CCAGTGGGAA 
GATCCCAGAT TGCAGAACCC AGCTATTACT GGTCCAGCTG TTCCCTATTC CAGAGAGTTC 
AAACAGAAAT ATGACTACTT CAGAAAGAAA TTAAAGAAAC CAGCTGATCT ACCAAACAGA 
TTTGAGATGA AACTCCACAG AAATAATATT TTTGAGGAGT CCTATAGAAG AATCATGTCC 
GTGAAGAGAC CCGATGTATT AAAAGCCAGG CTCTGGATTG AATTTGAAGC GGAAAAAGGA 
CTTGATTATG GAGGTGTGGC CAGAGAATGG TTCTTTCTGT TGTCCAAGGA AATGTTTAAT 
CCTTATTATG GTCTTTTTGA ATATTCGGCA ACGGACAACT ATACTCTTCA GATTAATCCA 
AATTCAGGCC TTTGTAATGA AGATCATCTG TCCTATTTCA CATTTATTGG CCGGGTTGCT 
GGGCTGGCAG TGTATCATGG GAAGTTATTA GATGGCTTTT TCATCAGGCC TTTTTATAAG 
ATGATGCTGG GGAAACCAAT AACTCTGAAA GACATGGAAT CAGTGGATAG TGAATATTAC 
AATTCCTTGA AATGGATCCT GGAAAATGAC CCAACAGAGC TGGATCTCAT GTTTTGCATA 
GATGAGGAAA ACTTTGGACA GACTTATCAA GTGGACTTGA AGCCTAATGG ATCAGAAATC 
ATGGTAACAA ATGAGAACAA ACGGGAATAT ATAGACTTAG TCATCCAATG GAGGTTTGTA 
AACAGAGTTC AGAAACAAAT GAATGCTTTT TTGGAGGGAT TCACAGAACT CCTACCTACT 
GATTTGATTA AAATTTTTGA TGAAAATGAG CTGGAGTTAC TAATGTGTGG CCTTGGAGAT 
GTTGATGTTA ATGACTGGAG ACAACATACG ATCTACAAGA ACGGATACTG TCCGAACCAT 
CCTTTAATCC AGTGGTTCTG GAAGGCTGTG TTACTGATGG ATGCTGAGAA ACGTATCCGG 
TTACTGCAGT TTGTTACGGG GACATCTCGA GTGCCTATGA ATGGATTTGC TGAACTTTAC 
GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GAAGTCCTGA TAAATTGCCC 
AGAGCTCACA CATGCTTTAA TCGCCTTGAC TTACCTCCTT ATGAATCTTT TGAAGATTTA 
CGAGAGAAAC TTCTTATGGC AGTGGAAAAT GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054255.1
CDS	199..2895
Translation	METRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198470.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198470.1

ORF Insert Sequence:

ATGGAGACTA GAGACGACTT CCTTGGTCAA GTAGACGTCC CACTTAATCA TCTTCCGACA 
GAAGACCCAA CCATGGAACG GCCATACACG TTTAAGGATT TTCTTCTCAG ACCAAGAAGC 
CACAAATCTC GGGTAAAGGG TTTCTTGAGA CTGAAGATGG CCTATATGCC GAAAAATGGA 
GGACAAGAGG ATGAAAATAA TGATCAAAGA GATGACTCGG AGCATGGGTG GGAAGTGGTT 
GATTCCAATG ATTCTGCATC TCATCGCCAA GAGGAGTTAC CGCCACCTCC ACTGCCTCCT 
GGATGGGAAG AAAAAGTGGA CAATCTTGGT CGTACTTACT ATGTCAACCA CAACAACAGG 
ACAACTCAGT GGCACCGGCC AACTTTAATT GATATGGCAT CTGAATCAGA CAACAACATT 
AGACAAATAA ACCAAGAGGC GGCTCATAGA CGCTTTCGAT CTCGGAGACA CATTAGTGAA 
GATTTGGAGC CAGAGCCCAT GGACAGTGGA GATATACCTG AACCCTGGGA AACTATTTCA 
GAGGAAGTGA GTGCAAGTGG AGATTCCTTA AGCCTGTCCT TGCCACCTCC CCCGGCCTCT 
CCAGTTTCTC GGACCAGTCC CCAGGAGCTG TCTGATGAAT TGAACAGAAG GCTCCAAGTC 
ACTCCTGATT CCAATGGGGA ACAACTCAGT TCTTTGATAC AAAGAGATCC TTCATCAAGA 
TTAAGGTCTT GCAGCGTAAC AGATGCAGTT GCTGAACAGT CTCAATTATC ACTGCAGAGT 
GGTCCATCTA GGAGAGCTCG TTCTTCAACT GTCACAGGTG GTGAAGAACC AACGCCATCA 
GTGGCCTATG TGCATACAAC ACCGGGCTTA CCTTCAGGCT GGGAAGAAAG GAAAGATGCT 
AAGGGACGTA CTTATTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCCATT 
ATGCAGCTTG CAGAAGATGG TGCAGTTGGG TCAGCAACAA ACAGTAACAA CCATCTCAGT 
GAACCTCAGA TAAGACGGCC TCGTAGCCTC AGTTCACCCA CAGTAACTTT ATCTGCTCCA 
CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA CGCCGAGCTG TAAAGGATAC CCTTTCGAAT 
CCTCAGTCTC CCCAGCCCTC ACCTTATAAT TCTCCTAAAC CACAACACAA AGTGACACAG 
AGCTTCTTGC CCCCTGGCTG GGAGATGCGA ATAGCTCCAA ATGGCAGGCC CTTCTTCATT 
GATCACAATA CTAAAACCAC TACCTGGGAA GATCCACGAC TGAAATTTCC AGTACATTTG 
AGATCAAAAG CTTCTTTGAA CCCTAATGAT TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA 
AGAATACATT TGGATGGAAG AACGTTTTAT ATAGACCATA GAAGCCAGGT GTTGATATGT 
GGAGACATTG ATACCAGAGA CTTAGTGCCC TCATACGATA ATAAAATTAC CCAGTGGGAA 
GATCCCAGAT TGCAGAACCC AGCTATTACT GGTCCAGCTG TTCCCTATTC CAGAGAGTTC 
AAACAGAAAT ATGACTACTT CAGAAAGAAA TTAAAGAAAC CAGCTGATCT ACCAAACAGA 
TTTGAGATGA AACTCCACAG AAATAATATT TTTGAGGAGT CCTATAGAAG AATCATGTCC 
GTGAAGAGAC CCGATGTATT AAAAGCCAGG CTCTGGATTG AATTTGAAGC GGAAAAAGGA 
CTTGATTATG GAGGTGTGGC CAGAGAATGG TTCTTTCTGT TGTCCAAGGA AATGTTTAAT 
CCTTATTATG GTCTTTTTGA ATATTCGGCA ACGGACAACT ATACTCTTCA GATTAATCCA 
AATTCAGGCC TTTGTAATGA AGATCATCTG TCCTATTTCA CATTTATTGG CCGGGTTGCT 
GGGCTGGCAG TGTATCATGG GAAGTTATTA GATGGCTTTT TCATCAGGCC TTTTTATAAG 
ATGATGCTGG GGAAACCAAT AACTCTGAAA GACATGGAAT CAGTGGATAG TGAATATTAC 
AATTCCTTGA AATGGATCCT GGAAAATGAC CCAACAGAGC TGGATCTCAT GTTTTGCATA 
GATGAGGAAA ACTTTGGACA GACTTATCAA GTGGACTTGA AGCCTAATGG ATCAGAAATC 
ATGGTAACAA ATGAGAACAA ACGGGAATAT ATAGACTTAG TCATCCAATG GAGGTTTGTA 
AACAGAGTTC AGAAACAAAT GAATGCTTTT TTGGAGGGAT TCACAGAACT CCTACCTACT 
GATTTGATTA AAATTTTTGA TGAAAATGAG CTGGAGTTAC TAATGTGTGG CCTTGGAGAT 
GTTGATGTTA ATGACTGGAG ACAACATACG ATCTACAAGA ACGGATACTG TCCGAACCAT 
CCTTTAATCC AGTGGTTCTG GAAGGCTGTG TTACTGATGG ATGCTGAGAA ACGTATCCGG 
TTACTGCAGT TTGTTACGGG GACATCTCGA GTGCCTATGA ATGGATTTGC TGAACTTTAC 
GGTTCAAATG GTCCTCAGCT GTTTACAATA GAGCAATGGG GAAGTCCTGA TAAATTGCCC 
AGAGCTCACA CATGCTTTAA TCGCCTTGAC TTACCTCCTT ATGAATCTTT TGAAGATTTA 
CGAGAGAAAC TTCTTATGGC AGTGGAAAAT GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198471.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054256.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OAl83313
Clone ID Related Accession (Same CDS sequence)	XM_025198471.1 , XM_025198472.1 , XM_025198473.1
Accession Version	XM_025198471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054256.1
CDS	376..3000
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198471.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198471.1

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198472.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054257.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OAl83313
Clone ID Related Accession (Same CDS sequence)	XM_025198471.1 , XM_025198472.1 , XM_025198473.1
Accession Version	XM_025198472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054257.1
CDS	190..2814
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198472.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198472.1

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198473.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054258.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OAl83313
Clone ID Related Accession (Same CDS sequence)	XM_025198471.1 , XM_025198472.1 , XM_025198473.1
Accession Version	XM_025198473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054258.1
CDS	448..3072
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198473.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198473.1

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCAGAGTGG TCCATCTAGG 
AGAGCTCGTT CTTCAACTGT CACAGGTGGT GAAGAACCAA CGCCATCAGT GGCCTATGTG 
CATACAACAC CGGGCTTACC TTCAGGCTGG GAAGAAAGGA AAGATGCTAA GGGACGTACT 
TATTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCCATTAT GCAGCTTGCA 
GAAGATGGTG CAGTTGGGTC AGCAACAAAC AGTAACAACC ATCTCAGTGA ACCTCAGATA 
AGACGGCCTC GTAGCCTCAG TTCACCCACA GTAACTTTAT CTGCTCCACT TGAGGGACTC 
TCCAAGGACT CTCCTGCACG CCGAGCTGTA AAGGATACCC TTTCGAATCC TCAGTCTCCC 
CAGCCCTCAC CTTATAATTC TCCTAAACCA CAACACAAAG TGACACAGAG CTTCTTGCCC 
CCTGGCTGGG AGATGCGAAT AGCTCCAAAT GGCAGGCCCT TCTTCATTGA TCACAATACT 
AAAACCACTA CCTGGGAAGA TCCACGACTG AAATTTCCAG TACATTTGAG ATCAAAAGCT 
TCTTTGAACC CTAATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG 
GATGGAAGAA CGTTTTATAT AGACCATAGA AGCCAGGTGT TGATATGTGG AGACATTGAT 
ACCAGAGACT TAGTGCCCTC ATACGATAAT AAAATTACCC AGTGGGAAGA TCCCAGATTG 
CAGAACCCAG CTATTACTGG TCCAGCTGTT CCCTATTCCA GAGAGTTCAA ACAGAAATAT 
GACTACTTCA GAAAGAAATT AAAGAAACCA GCTGATCTAC CAAACAGATT TGAGATGAAA 
CTCCACAGAA ATAATATTTT TGAGGAGTCC TATAGAAGAA TCATGTCCGT GAAGAGACCC 
GATGTATTAA AAGCCAGGCT CTGGATTGAA TTTGAAGCGG AAAAAGGACT TGATTATGGA 
GGTGTGGCCA GAGAATGGTT CTTTCTGTTG TCCAAGGAAA TGTTTAATCC TTATTATGGT 
CTTTTTGAAT ATTCGGCAAC GGACAACTAT ACTCTTCAGA TTAATCCAAA TTCAGGCCTT 
TGTAATGAAG ATCATCTGTC CTATTTCACA TTTATTGGCC GGGTTGCTGG GCTGGCAGTG 
TATCATGGGA AGTTATTAGA TGGCTTTTTC ATCAGGCCTT TTTATAAGAT GATGCTGGGG 
AAACCAATAA CTCTGAAAGA CATGGAATCA GTGGATAGTG AATATTACAA TTCCTTGAAA 
TGGATCCTGG AAAATGACCC AACAGAGCTG GATCTCATGT TTTGCATAGA TGAGGAAAAC 
TTTGGACAGA CTTATCAAGT GGACTTGAAG CCTAATGGAT CAGAAATCAT GGTAACAAAT 
GAGAACAAAC GGGAATATAT AGACTTAGTC ATCCAATGGA GGTTTGTAAA CAGAGTTCAG 
AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTCC TACCTACTGA TTTGATTAAA 
ATTTTTGATG AAAATGAGCT GGAGTTACTA ATGTGTGGCC TTGGAGATGT TGATGTTAAT 
GACTGGAGAC AACATACGAT CTACAAGAAC GGATACTGTC CGAACCATCC TTTAATCCAG 
TGGTTCTGGA AGGCTGTGTT ACTGATGGAT GCTGAGAAAC GTATCCGGTT ACTGCAGTTT 
GTTACGGGGA CATCTCGAGT GCCTATGAAT GGATTTGCTG AACTTTACGG TTCAAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGATA AATTGCCCAG AGCTCACACA 
TGCTTTAATC GCCTTGACTT ACCTCCTTAT GAATCTTTTG AAGATTTACG AGAGAAACTT 
CTTATGGCAG TGGAAAATGC TCAAGGATTT GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198474.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054259.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OAl83314
Clone ID Related Accession (Same CDS sequence)	XM_025198474.1
Accession Version	XM_025198474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCTTGCAGA AGATGGTGCA 
GTTGGGTCAG CAACAAACAG TAACAACCAT CTCAGTGAAC CTCAGATAAG ACGGCCTCGT 
AGCCTCAGTT CACCCACAGT AACTTTATCT GCTCCACTTG AGGGACTCTC CAAGGACTCT 
CCTGCACGCC GAGCTGTAAA GGATACCCTT TCGAATCCTC AGTCTCCCCA GCCCTCACCT 
TATAATTCTC CTAAACCACA ACACAAAGTG ACACAGAGCT TCTTGCCCCC TGGCTGGGAG 
ATGCGAATAG CTCCAAATGG CAGGCCCTTC TTCATTGATC ACAATACTAA AACCACTACC 
TGGGAAGATC CACGACTGAA ATTTCCAGTA CATTTGAGAT CAAAAGCTTC TTTGAACCCT 
AATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAACG 
TTTTATATAG ACCATAATAA TAAAATTACC CAGTGGGAAG ATCCCAGATT GCAGAACCCA 
GCTATTACTG GTCCAGCTGT TCCCTATTCC AGAGAGTTCA AACAGAAATA TGACTACTTC 
AGAAAGAAAT TAAAGAAACC AGCTGATCTA CCAAACAGAT TTGAGATGAA ACTCCACAGA 
AATAATATTT TTGAGGAGTC CTATAGAAGA ATCATGTCCG TGAAGAGACC CGATGTATTA 
AAAGCCAGGC TCTGGATTGA ATTTGAAGCG GAAAAAGGAC TTGATTATGG AGGTGTGGCC 
AGAGAATGGT TCTTTCTGTT GTCCAAGGAA ATGTTTAATC CTTATTATGG TCTTTTTGAA 
TATTCGGCAA CGGACAACTA TACTCTTCAG ATTAATCCAA ATTCAGGCCT TTGTAATGAA 
GATCATCTGT CCTATTTCAC ATTTATTGGC CGGGTTGCTG GGCTGGCAGT GTATCATGGG 
AAGTTATTAG ATGGCTTTTT CATCAGGCCT TTTTATAAGA TGATGCTGGG GAAACCAATA 
ACTCTGAAAG ACATGGAATC AGTGGATAGT GAATATTACA ATTCCTTGAA ATGGATCCTG 
GAAAATGACC CAACAGAGCT GGATCTCATG TTTTGCATAG ATGAGGAAAA CTTTGGACAG 
ACTTATCAAG TGGACTTGAA GCCTAATGGA TCAGAAATCA TGGTAACAAA TGAGAACAAA 
CGGGAATATA TAGACTTAGT CATCCAATGG AGGTTTGTAA ACAGAGTTCA GAAACAAATG 
AATGCTTTTT TGGAGGGATT CACAGAACTC CTACCTACTG ATTTGATTAA AATTTTTGAT 
GAAAATGAGC TGGAGTTACT AATGTGTGGC CTTGGAGATG TTGATGTTAA TGACTGGAGA 
CAACATACGA TCTACAAGAA CGGATACTGT CCGAACCATC CTTTAATCCA GTGGTTCTGG 
AAGGCTGTGT TACTGATGGA TGCTGAGAAA CGTATCCGGT TACTGCAGTT TGTTACGGGG 
ACATCTCGAG TGCCTATGAA TGGATTTGCT GAACTTTACG GTTCAAATGG TCCTCAGCTG 
TTTACAATAG AGCAATGGGG AAGTCCTGAT AAATTGCCCA GAGCTCACAC ATGCTTTAAT 
CGCCTTGACT TACCTCCTTA TGAATCTTTT GAAGATTTAC GAGAGAAACT TCTTATGGCA 
GTGGAAAATG CTCAAGGATT TGAAGGAGTG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054259.1
CDS	448..2823
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198474.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198474.1

ORF Insert Sequence:

ATGGAACGGC CATACACGTT TAAGGATTTT CTTCTCAGAC CAAGAAGCCA CAAATCTCGG 
GTAAAGGGTT TCTTGAGACT GAAGATGGCC TATATGCCGA AAAATGGAGG ACAAGAGGAT 
GAAAATAATG ATCAAAGAGA TGACTCGGAG CATGGGTGGG AAGTGGTTGA TTCCAATGAT 
TCTGCATCTC ATCGCCAAGA GGAGTTACCG CCACCTCCAC TGCCTCCTGG ATGGGAAGAA 
AAAGTGGACA ATCTTGGTCG TACTTACTAT GTCAACCACA ACAACAGGAC AACTCAGTGG 
CACCGGCCAA CTTTAATTGA TATGGCATCT GAATCAGACA ACAACATTAG ACAAATAAAC 
CAAGAGGCGG CTCATAGACG CTTTCGATCT CGGAGACACA TTAGTGAAGA TTTGGAGCCA 
GAGCCCATGG ACAGTGGAGA TATACCTGAA CCCTGGGAAA CTATTTCAGA GGAAGTGAGT 
GCAAGTGGAG ATTCCTTAAG CCTGTCCTTG CCACCTCCCC CGGCCTCTCC AGTTTCTCGG 
ACCAGTCCCC AGGAGCTGTC TGATGAATTG AACAGAAGGC TCCAAGTCAC TCCTGATTCC 
AATGGGGAAC AACTCAGTTC TTTGATACAA AGAGATCCTT CATCAAGATT AAGGTCTTGC 
AGCGTAACAG ATGCAGTTGC TGAACAGTCT CAATTATCAC TGCTTGCAGA AGATGGTGCA 
GTTGGGTCAG CAACAAACAG TAACAACCAT CTCAGTGAAC CTCAGATAAG ACGGCCTCGT 
AGCCTCAGTT CACCCACAGT AACTTTATCT GCTCCACTTG AGGGACTCTC CAAGGACTCT 
CCTGCACGCC GAGCTGTAAA GGATACCCTT TCGAATCCTC AGTCTCCCCA GCCCTCACCT 
TATAATTCTC CTAAACCACA ACACAAAGTG ACACAGAGCT TCTTGCCCCC TGGCTGGGAG 
ATGCGAATAG CTCCAAATGG CAGGCCCTTC TTCATTGATC ACAATACTAA AACCACTACC 
TGGGAAGATC CACGACTGAA ATTTCCAGTA CATTTGAGAT CAAAAGCTTC TTTGAACCCT 
AATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAACG 
TTTTATATAG ACCATAATAA TAAAATTACC CAGTGGGAAG ATCCCAGATT GCAGAACCCA 
GCTATTACTG GTCCAGCTGT TCCCTATTCC AGAGAGTTCA AACAGAAATA TGACTACTTC 
AGAAAGAAAT TAAAGAAACC AGCTGATCTA CCAAACAGAT TTGAGATGAA ACTCCACAGA 
AATAATATTT TTGAGGAGTC CTATAGAAGA ATCATGTCCG TGAAGAGACC CGATGTATTA 
AAAGCCAGGC TCTGGATTGA ATTTGAAGCG GAAAAAGGAC TTGATTATGG AGGTGTGGCC 
AGAGAATGGT TCTTTCTGTT GTCCAAGGAA ATGTTTAATC CTTATTATGG TCTTTTTGAA 
TATTCGGCAA CGGACAACTA TACTCTTCAG ATTAATCCAA ATTCAGGCCT TTGTAATGAA 
GATCATCTGT CCTATTTCAC ATTTATTGGC CGGGTTGCTG GGCTGGCAGT GTATCATGGG 
AAGTTATTAG ATGGCTTTTT CATCAGGCCT TTTTATAAGA TGATGCTGGG GAAACCAATA 
ACTCTGAAAG ACATGGAATC AGTGGATAGT GAATATTACA ATTCCTTGAA ATGGATCCTG 
GAAAATGACC CAACAGAGCT GGATCTCATG TTTTGCATAG ATGAGGAAAA CTTTGGACAG 
ACTTATCAAG TGGACTTGAA GCCTAATGGA TCAGAAATCA TGGTAACAAA TGAGAACAAA 
CGGGAATATA TAGACTTAGT CATCCAATGG AGGTTTGTAA ACAGAGTTCA GAAACAAATG 
AATGCTTTTT TGGAGGGATT CACAGAACTC CTACCTACTG ATTTGATTAA AATTTTTGAT 
GAAAATGAGC TGGAGTTACT AATGTGTGGC CTTGGAGATG TTGATGTTAA TGACTGGAGA 
CAACATACGA TCTACAAGAA CGGATACTGT CCGAACCATC CTTTAATCCA GTGGTTCTGG 
AAGGCTGTGT TACTGATGGA TGCTGAGAAA CGTATCCGGT TACTGCAGTT TGTTACGGGG 
ACATCTCGAG TGCCTATGAA TGGATTTGCT GAACTTTACG GTTCAAATGG TCCTCAGCTG 
TTTACAATAG AGCAATGGGG AAGTCCTGAT AAATTGCCCA GAGCTCACAC ATGCTTTAAT 
CGCCTTGACT TACCTCCTTA TGAATCTTTT GAAGATTTAC GAGAGAAACT TCTTATGGCA 
GTGGAAAATG CTCAAGGATT TGAAGGAGTG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198462.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054247.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAl83304
Clone ID Related Accession (Same CDS sequence)	XM_025198462.1
Accession Version	XM_025198462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GGTTCAAATG GTCCTCAGCT GTTTACAATA 
GAGCAATGGG GAAGTCCTGA TAAATTGCCC AGAGCTCACA CATGCTTTAA TCGCCTTGAC 
TTACCTCCTT ATGAATCTTT TGAAGATTTA CGAGAGAAAC TTCTTATGGC AGTGGAAAAT 
GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054247.1
CDS	285..4151
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198462.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198462.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GGTTCAAATG GTCCTCAGCT GTTTACAATA 
GAGCAATGGG GAAGTCCTGA TAAATTGCCC AGAGCTCACA CATGCTTTAA TCGCCTTGAC 
TTACCTCCTT ATGAATCTTT TGAAGATTTA CGAGAGAAAC TTCTTATGGC AGTGGAAAAT 
GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198463.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054248.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

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CloneID	OAl83305
Clone ID Related Accession (Same CDS sequence)	XM_025198463.1
Accession Version	XM_025198463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GCTTTAATCG CCTTGACTTA CCTCCTTATG 
AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT CAAGGATTTG 
AAGGAGTGGA TTAAAACAGC TACTTTTTTT TCTTTTTCCA TTGAGCAAGT TCTGCTTGCA 
CTTTTGAATT TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054248.1
CDS	286..4143
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYALIALTYLLMNLLKIYERNFLWQWKMLKDLKEWIKTATFFSFSIEQVLLALLNFA

Target ORF information:

RefSeq Version	XM_025198463.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198463.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GCTTTAATCG CCTTGACTTA CCTCCTTATG 
AATCTTTTGA AGATTTACGA GAGAAACTTC TTATGGCAGT GGAAAATGCT CAAGGATTTG 
AAGGAGTGGA TTAAAACAGC TACTTTTTTT TCTTTTTCCA TTGAGCAAGT TCTGCTTGCA 
CTTTTGAATT TTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198464.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054249.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAl83306
Clone ID Related Accession (Same CDS sequence)	XM_025198464.1
Accession Version	XM_025198464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA ATAATAAAAT TACCCAGTGG GAAGATCCCA GATTGCAGAA CCCAGCTATT 
ACTGGTCCAG CTGTTCCCTA TTCCAGAGAG TTCAAACAGA AATATGACTA CTTCAGAAAG 
AAATTAAAGA AACCAGCTGA TCTACCAAAC AGATTTGAGA TGAAACTCCA CAGAAATAAT 
ATTTTTGAGG AGTCCTATAG AAGAATCATG TCCGTGAAGA GACCCGATGT ATTAAAAGCC 
AGGCTCTGGA TTGAATTTGA AGCGGAAAAA GGACTTGATT ATGGAGGTGT GGCCAGAGAA 
TGGTTCTTTC TGTTGTCCAA GGAAATGTTT AATCCTTATT ATGGTCTTTT TGAATATTCG 
GCAACGGACA ACTATACTCT TCAGATTAAT CCAAATTCAG GCCTTTGTAA TGAAGATCAT 
CTGTCCTATT TCACATTTAT TGGCCGGGTT GCTGGGCTGG CAGTGTATCA TGGGAAGTTA 
TTAGATGGCT TTTTCATCAG GCCTTTTTAT AAGATGATGC TGGGGAAACC AATAACTCTG 
AAAGACATGG AATCAGTGGA TAGTGAATAT TACAATTCCT TGAAATGGAT CCTGGAAAAT 
GACCCAACAG AGCTGGATCT CATGTTTTGC ATAGATGAGG AAAACTTTGG ACAGACTTAT 
CAAGTGGACT TGAAGCCTAA TGGATCAGAA ATCATGGTAA CAAATGAGAA CAAACGGGAA 
TATATAGACT TAGTCATCCA ATGGAGGTTT GTAAACAGAG TTCAGAAACA AATGAATGCT 
TTTTTGGAGG GATTCACAGA ACTCCTACCT ACTGATTTGA TTAAAATTTT TGATGAAAAT 
GAGCTGGAGT TACTAATGTG TGGCCTTGGA GATGTTGATG TTAATGACTG GAGACAACAT 
ACGATCTACA AGAACGGATA CTGTCCGAAC CATCCTTTAA TCCAGTGGTT CTGGAAGGCT 
GTGTTACTGA TGGATGCTGA GAAACGTATC CGGTTACTGC AGTTTGTTAC GGGGACATCT 
CGAGTGCCTA TGAATGGATT TGCTGAACTT TACGGTTCAA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGATAAATTG CCCAGAGCTC ACACATGCTT TAATCGCCTT 
GACTTACCTC CTTATGAATC TTTTGAAGAT TTACGAGAGA AACTTCTTAT GGCAGTGGAA 
AATGCTCAAG GATTTGAAGG AGTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054249.1
CDS	1..3810
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198464.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198464.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA ATAATAAAAT TACCCAGTGG GAAGATCCCA GATTGCAGAA CCCAGCTATT 
ACTGGTCCAG CTGTTCCCTA TTCCAGAGAG TTCAAACAGA AATATGACTA CTTCAGAAAG 
AAATTAAAGA AACCAGCTGA TCTACCAAAC AGATTTGAGA TGAAACTCCA CAGAAATAAT 
ATTTTTGAGG AGTCCTATAG AAGAATCATG TCCGTGAAGA GACCCGATGT ATTAAAAGCC 
AGGCTCTGGA TTGAATTTGA AGCGGAAAAA GGACTTGATT ATGGAGGTGT GGCCAGAGAA 
TGGTTCTTTC TGTTGTCCAA GGAAATGTTT AATCCTTATT ATGGTCTTTT TGAATATTCG 
GCAACGGACA ACTATACTCT TCAGATTAAT CCAAATTCAG GCCTTTGTAA TGAAGATCAT 
CTGTCCTATT TCACATTTAT TGGCCGGGTT GCTGGGCTGG CAGTGTATCA TGGGAAGTTA 
TTAGATGGCT TTTTCATCAG GCCTTTTTAT AAGATGATGC TGGGGAAACC AATAACTCTG 
AAAGACATGG AATCAGTGGA TAGTGAATAT TACAATTCCT TGAAATGGAT CCTGGAAAAT 
GACCCAACAG AGCTGGATCT CATGTTTTGC ATAGATGAGG AAAACTTTGG ACAGACTTAT 
CAAGTGGACT TGAAGCCTAA TGGATCAGAA ATCATGGTAA CAAATGAGAA CAAACGGGAA 
TATATAGACT TAGTCATCCA ATGGAGGTTT GTAAACAGAG TTCAGAAACA AATGAATGCT 
TTTTTGGAGG GATTCACAGA ACTCCTACCT ACTGATTTGA TTAAAATTTT TGATGAAAAT 
GAGCTGGAGT TACTAATGTG TGGCCTTGGA GATGTTGATG TTAATGACTG GAGACAACAT 
ACGATCTACA AGAACGGATA CTGTCCGAAC CATCCTTTAA TCCAGTGGTT CTGGAAGGCT 
GTGTTACTGA TGGATGCTGA GAAACGTATC CGGTTACTGC AGTTTGTTAC GGGGACATCT 
CGAGTGCCTA TGAATGGATT TGCTGAACTT TACGGTTCAA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGATAAATTG CCCAGAGCTC ACACATGCTT TAATCGCCTT 
GACTTACCTC CTTATGAATC TTTTGAAGAT TTACGAGAGA AACTTCTTAT GGCAGTGGAA 
AATGCTCAAG GATTTGAAGG AGTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198465.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054250.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAl83307
Clone ID Related Accession (Same CDS sequence)	XM_025198465.1
Accession Version	XM_025198465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GGTTCAAATG GTCCTCAGCT GTTTACAATA 
GAGCAATGGG GAAGTCCTGA TAAATTGCCC AGAGCTCACA CATGCTTTAA TCGCCTTGAC 
TTACCTCCTT ATGAATCTTT TGAAGATTTA CGAGAGAAAC TTCTTATGGC AGTGGAAAAT 
GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054250.1
CDS	285..4091
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198465.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198465.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGGCTGTG 
TTACTGATGG ATGCTGAGAA ACGTATCCGG TTACTGCAGT TTGTTACGGG GACATCTCGA 
GTGCCTATGA ATGGATTTGC TGAACTTTAC GGTTCAAATG GTCCTCAGCT GTTTACAATA 
GAGCAATGGG GAAGTCCTGA TAAATTGCCC AGAGCTCACA CATGCTTTAA TCGCCTTGAC 
TTACCTCCTT ATGAATCTTT TGAAGATTTA CGAGAGAAAC TTCTTATGGC AGTGGAAAAT 
GCTCAAGGAT TTGAAGGAGT GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198466.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054251.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

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CloneID	OAl83308
Clone ID Related Accession (Same CDS sequence)	XM_025198466.1
Accession Version	XM_025198466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA ATAATAAAAT TACCCAGTGG GAAGATCCCA GATTGCAGAA CCCAGCTATT 
ACTGGTCCAG CTGTTCCCTA TTCCAGAGAG TTCAAACAGA AATATGACTA CTTCAGAAAG 
AAATTAAAGA AACCAGCTGA TCTACCAAAC AGATTTGAGA TGAAACTCCA CAGAAATAAT 
ATTTTTGAGG AGTCCTATAG AAGAATCATG TCCGTGAAGA GACCCGATGT ATTAAAAGCC 
AGGCTCTGGA TTGAATTTGA AGCGGAAAAA GGACTTGATT ATGGAGGTGT GGCCAGAGAA 
TGGTTCTTTC TGTTGTCCAA GGAAATGTTT AATCCTTATT ATGGTCTTTT TGAATATTCG 
GCAACGGACA ACTATACTCT TCAGATTAAT CCAAATTCAG GCCTTTGTAA TGAAGATCAT 
CTGTCCTATT TCACATTTAT TGGCCGGGTT GCTGGGCTGG CAGTGTATCA TGGGAAGTTA 
TTAGATGGCT TTTTCATCAG GCCTTTTTAT AAGATGATGC TGGGGAAACC AATAACTCTG 
AAAGACATGG AATCAGTGGA TAGTGAATAT TACAATTCCT TGAAATGGAT CCTGGAAAAT 
GACCCAACAG AGCTGGATCT CATGTTTTGC ATAGATGAGG AAAACTTTGG ACAGACTTAT 
CAAGTGGACT TGAAGCCTAA TGGATCAGAA ATCATGGTAA CAAATGAGAA CAAACGGGAA 
TATATAGACT TAGTCATCCA ATGGAGGTTT GTAAACAGAG TTCAGAAACA AATGAATGCT 
TTTTTGGAGG GATTCACAGA ACTCCTACCT ACTGATTTGA TTAAAATTTT TGATGAAAAT 
GAGCTGGAGT TACTAATGTG TGGCCTTGGA GATGTTGATG TTAATGACTG GAGACAACAT 
ACGATCTACA AGAACGGATA CTGTCCGAAC CATCCTTTAA TCCAGTGGTT CTGGAAGGCT 
GTGTTACTGA TGGATGCTGA GAAACGTATC CGGTTACTGC AGTTTGTTAC GGGGACATCT 
CGAGTGCCTA TGAATGGATT TGCTGAACTT TACGGTTCAA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGATAAATTG CCCAGAGCTC ACACATGCTT TAATCGCCTT 
GACTTACCTC CTTATGAATC TTTTGAAGAT TTACGAGAGA AACTTCTTAT GGCAGTGGAA 
AATGCTCAAG GATTTGAAGG AGTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054251.1
CDS	284..4033
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198466.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198466.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA ATAATAAAAT TACCCAGTGG GAAGATCCCA GATTGCAGAA CCCAGCTATT 
ACTGGTCCAG CTGTTCCCTA TTCCAGAGAG TTCAAACAGA AATATGACTA CTTCAGAAAG 
AAATTAAAGA AACCAGCTGA TCTACCAAAC AGATTTGAGA TGAAACTCCA CAGAAATAAT 
ATTTTTGAGG AGTCCTATAG AAGAATCATG TCCGTGAAGA GACCCGATGT ATTAAAAGCC 
AGGCTCTGGA TTGAATTTGA AGCGGAAAAA GGACTTGATT ATGGAGGTGT GGCCAGAGAA 
TGGTTCTTTC TGTTGTCCAA GGAAATGTTT AATCCTTATT ATGGTCTTTT TGAATATTCG 
GCAACGGACA ACTATACTCT TCAGATTAAT CCAAATTCAG GCCTTTGTAA TGAAGATCAT 
CTGTCCTATT TCACATTTAT TGGCCGGGTT GCTGGGCTGG CAGTGTATCA TGGGAAGTTA 
TTAGATGGCT TTTTCATCAG GCCTTTTTAT AAGATGATGC TGGGGAAACC AATAACTCTG 
AAAGACATGG AATCAGTGGA TAGTGAATAT TACAATTCCT TGAAATGGAT CCTGGAAAAT 
GACCCAACAG AGCTGGATCT CATGTTTTGC ATAGATGAGG AAAACTTTGG ACAGACTTAT 
CAAGTGGACT TGAAGCCTAA TGGATCAGAA ATCATGGTAA CAAATGAGAA CAAACGGGAA 
TATATAGACT TAGTCATCCA ATGGAGGTTT GTAAACAGAG TTCAGAAACA AATGAATGCT 
TTTTTGGAGG GATTCACAGA ACTCCTACCT ACTGATTTGA TTAAAATTTT TGATGAAAAT 
GAGCTGGAGT TACTAATGTG TGGCCTTGGA GATGTTGATG TTAATGACTG GAGACAACAT 
ACGATCTACA AGAACGGATA CTGTCCGAAC CATCCTTTAA TCCAGTGGTT CTGGAAGGCT 
GTGTTACTGA TGGATGCTGA GAAACGTATC CGGTTACTGC AGTTTGTTAC GGGGACATCT 
CGAGTGCCTA TGAATGGATT TGCTGAACTT TACGGTTCAA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGATAAATTG CCCAGAGCTC ACACATGCTT TAATCGCCTT 
GACTTACCTC CTTATGAATC TTTTGAAGAT TTACGAGAGA AACTTCTTAT GGCAGTGGAA 
AATGCTCAAG GATTTGAAGG AGTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198467.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054252.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAl83309
Clone ID Related Accession (Same CDS sequence)	XM_025198467.1
Accession Version	XM_025198467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCTTGCA GAAGATGGTG CAGTTGGGTC AGCAACAAAC 
AGTAACAACC ATCTCAGTGA ACCTCAGATA AGACGGCCTC GTAGCCTCAG TTCACCCACA 
GTAACTTTAT CTGCTCCACT TGAGGGACTC TCCAAGGACT CTCCTGCACG CCGAGCTGTA 
AAGGATACCC TTTCGAATCC TCAGTCTCCC CAGCCCTCAC CTTATAATTC TCCTAAACCA 
CAACACAAAG TGACACAGAG CTTCTTGCCC CCTGGCTGGG AGATGCGAAT AGCTCCAAAT 
GGCAGGCCCT TCTTCATTGA TCACAATACT AAAACCACTA CCTGGGAAGA TCCACGACTG 
AAATTTCCAG TACATTTGAG ATCAAAAGCT TCTTTGAACC CTAATGATTT GGGCCCTCTT 
CCTCCTGGTT GGGAGGAAAG AATACATTTG GATGGAAGAA CGTTTTATAT AGACCATAGA 
AGCCAGGTGT TGATATGTGG AGACATTGAT ACCAGAGACT TAGTGCCCTC ATACGATAAT 
AAAATTACCC AGTGGGAAGA TCCCAGATTG CAGAACCCAG CTATTACTGG TCCAGCTGTT 
CCCTATTCCA GAGAGTTCAA ACAGAAATAT GACTACTTCA GAAAGAAATT AAAGAAACCA 
GCTGATCTAC CAAACAGATT TGAGATGAAA CTCCACAGAA ATAATATTTT TGAGGAGTCC 
TATAGAAGAA TCATGTCCGT GAAGAGACCC GATGTATTAA AAGCCAGGCT CTGGATTGAA 
TTTGAAGCGG AAAAAGGACT TGATTATGGA GGTGTGGCCA GAGAATGGTT CTTTCTGTTG 
TCCAAGGAAA TGTTTAATCC TTATTATGGT CTTTTTGAAT ATTCGGCAAC GGACAACTAT 
ACTCTTCAGA TTAATCCAAA TTCAGGCCTT TGTAATGAAG ATCATCTGTC CTATTTCACA 
TTTATTGGCC GGGTTGCTGG GCTGGCAGTG TATCATGGGA AGTTATTAGA TGGCTTTTTC 
ATCAGGCCTT TTTATAAGAT GATGCTGGGG AAACCAATAA CTCTGAAAGA CATGGAATCA 
GTGGATAGTG AATATTACAA TTCCTTGAAA TGGATCCTGG AAAATGACCC AACAGAGCTG 
GATCTCATGT TTTGCATAGA TGAGGAAAAC TTTGGACAGA CTTATCAAGT GGACTTGAAG 
CCTAATGGAT CAGAAATCAT GGTAACAAAT GAGAACAAAC GGGAATATAT AGACTTAGTC 
ATCCAATGGA GGTTTGTAAA CAGAGTTCAG AAACAAATGA ATGCTTTTTT GGAGGGATTC 
ACAGAACTCC TACCTACTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTACTA 
ATGTGTGGCC TTGGAGATGT TGATGTTAAT GACTGGAGAC AACATACGAT CTACAAGAAC 
GGATACTGTC CGAACCATCC TTTAATCCAG TGGTTCTGGA AGGCTGTGTT ACTGATGGAT 
GCTGAGAAAC GTATCCGGTT ACTGCAGTTT GTTACGGGGA CATCTCGAGT GCCTATGAAT 
GGATTTGCTG AACTTTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGATA AATTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCTTAT 
GAATCTTTTG AAGATTTACG AGAGAAACTT CTTATGGCAG TGGAAAATGC TCAAGGATTT 
GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054252.1
CDS	287..4021
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198467.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198467.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCTTGCA GAAGATGGTG CAGTTGGGTC AGCAACAAAC 
AGTAACAACC ATCTCAGTGA ACCTCAGATA AGACGGCCTC GTAGCCTCAG TTCACCCACA 
GTAACTTTAT CTGCTCCACT TGAGGGACTC TCCAAGGACT CTCCTGCACG CCGAGCTGTA 
AAGGATACCC TTTCGAATCC TCAGTCTCCC CAGCCCTCAC CTTATAATTC TCCTAAACCA 
CAACACAAAG TGACACAGAG CTTCTTGCCC CCTGGCTGGG AGATGCGAAT AGCTCCAAAT 
GGCAGGCCCT TCTTCATTGA TCACAATACT AAAACCACTA CCTGGGAAGA TCCACGACTG 
AAATTTCCAG TACATTTGAG ATCAAAAGCT TCTTTGAACC CTAATGATTT GGGCCCTCTT 
CCTCCTGGTT GGGAGGAAAG AATACATTTG GATGGAAGAA CGTTTTATAT AGACCATAGA 
AGCCAGGTGT TGATATGTGG AGACATTGAT ACCAGAGACT TAGTGCCCTC ATACGATAAT 
AAAATTACCC AGTGGGAAGA TCCCAGATTG CAGAACCCAG CTATTACTGG TCCAGCTGTT 
CCCTATTCCA GAGAGTTCAA ACAGAAATAT GACTACTTCA GAAAGAAATT AAAGAAACCA 
GCTGATCTAC CAAACAGATT TGAGATGAAA CTCCACAGAA ATAATATTTT TGAGGAGTCC 
TATAGAAGAA TCATGTCCGT GAAGAGACCC GATGTATTAA AAGCCAGGCT CTGGATTGAA 
TTTGAAGCGG AAAAAGGACT TGATTATGGA GGTGTGGCCA GAGAATGGTT CTTTCTGTTG 
TCCAAGGAAA TGTTTAATCC TTATTATGGT CTTTTTGAAT ATTCGGCAAC GGACAACTAT 
ACTCTTCAGA TTAATCCAAA TTCAGGCCTT TGTAATGAAG ATCATCTGTC CTATTTCACA 
TTTATTGGCC GGGTTGCTGG GCTGGCAGTG TATCATGGGA AGTTATTAGA TGGCTTTTTC 
ATCAGGCCTT TTTATAAGAT GATGCTGGGG AAACCAATAA CTCTGAAAGA CATGGAATCA 
GTGGATAGTG AATATTACAA TTCCTTGAAA TGGATCCTGG AAAATGACCC AACAGAGCTG 
GATCTCATGT TTTGCATAGA TGAGGAAAAC TTTGGACAGA CTTATCAAGT GGACTTGAAG 
CCTAATGGAT CAGAAATCAT GGTAACAAAT GAGAACAAAC GGGAATATAT AGACTTAGTC 
ATCCAATGGA GGTTTGTAAA CAGAGTTCAG AAACAAATGA ATGCTTTTTT GGAGGGATTC 
ACAGAACTCC TACCTACTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTACTA 
ATGTGTGGCC TTGGAGATGT TGATGTTAAT GACTGGAGAC AACATACGAT CTACAAGAAC 
GGATACTGTC CGAACCATCC TTTAATCCAG TGGTTCTGGA AGGCTGTGTT ACTGATGGAT 
GCTGAGAAAC GTATCCGGTT ACTGCAGTTT GTTACGGGGA CATCTCGAGT GCCTATGAAT 
GGATTTGCTG AACTTTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGATA AATTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCTTAT 
GAATCTTTTG AAGATTTACG AGAGAAACTT CTTATGGCAG TGGAAAATGC TCAAGGATTT 
GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198468.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054253.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAl83310
Clone ID Related Accession (Same CDS sequence)	XM_025198468.1
Accession Version	XM_025198468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCTTGCA GAAGATGGTG CAGTTGGGTC AGCAACAAAC 
AGTAACAACC ATCTCAGTGA ACCTCAGATA AGACGGCCTC GTAGCCTCAG TTCACCCACA 
GTAACTTTAT CTGCTCCACT TGAGGGACTC TCCAAGGACT CTCCTGCACG CCGAGCTGTA 
AAGGATACCC TTTCGAATCC TCAGTCTCCC CAGCCCTCAC CTTATAATTC TCCTAAACCA 
CAACACAAAG TGACACAGAG CTTCTTGCCC CCTGGCTGGG AGATGCGAAT AGCTCCAAAT 
GGCAGGCCCT TCTTCATTGA TCACAATACT AAAACCACTA CCTGGGAAGA TCCACGACTG 
AAATTTCCAG TACATTTGAG ATCAAAAGCT TCTTTGAACC CTAATGATTT GGGCCCTCTT 
CCTCCTGGTT GGGAGGAAAG AATACATTTG GATGGAAGAA CGTTTTATAT AGACCATAGA 
AGCCAGGTGT TGATATGTGG AGACATTGAT ACCAGAGACT TAGTGCCCTC ATACGATAAT 
AAAATTACCC AGTGGGAAGA TCCCAGATTG CAGAACCCAG CTATTACTGG TCCAGCTGTT 
CCCTATTCCA GAGAGTTCAA ACAGAAATAT GACTACTTCA GAAAGAAATT AAAGAAACCA 
GCTGATCTAC CAAACAGATT TGAGATGAAA CTCCACAGAA ATAATATTTT TGAGGAGTCC 
TATAGAAGAA TCATGTCCGT GAAGAGACCC GATGTATTAA AAGCCAGGCT CTGGATTGAA 
TTTGAAGCGG AAAAAGGACT TGATTATGGA GGTGTGGCCA GAGAATGGTT CTTTCTGTTG 
TCCAAGGAAA TGTTTAATCC TTATTATGGT CTTTTTGAAT ATTCGGCAAC GGACAACTAT 
ACTCTTCAGA TTAATCCAAA TTCAGGCCTT TGTAATGAAG ATCATCTGTC CTATTTCACA 
TTTATTGGCC GGGTTGCTGG GCTGGCAGTG TATCATGGGA AGTTATTAGA TGGCTTTTTC 
ATCAGGCCTT TTTATAAGAT GATGCTGGGG AAACCAATAA CTCTGAAAGA CATGGAATCA 
GTGGATAGTG AATATTACAA TTCCTTGAAA TGGATCCTGG AAAATGACCC AACAGAGCTG 
GATCTCATGT TTTGCATAGA TGAGGAAAAC TTTGGACAGA CTTATCAAGT GGACTTGAAG 
CCTAATGGAT CAGAAATCAT GGTAACAAAT GAGAACAAAC GGGAATATAT AGACTTAGTC 
ATCCAATGGA GGTTTGTAAA CAGAGTTCAG AAACAAATGA ATGCTTTTTT GGAGGGATTC 
ACAGAACTCC TACCTACTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTACTA 
ATGTGTGGCC TTGGAGATGT TGATGTTAAT GACTGGAGAC AACATACGAT CTACAAGAAC 
GGATACTGTC CGAACCATCC TTTAATCCAG TGGTTCTGGA AGGCTGTGTT ACTGATGGAT 
GCTGAGAAAC GTATCCGGTT ACTGCAGTTT GTTACGGGGA CATCTCGAGT GCCTATGAAT 
GGATTTGCTG AACTTTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGATA AATTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCTTAT 
GAATCTTTTG AAGATTTACG AGAGAAACTT CTTATGGCAG TGGAAAATGC TCAAGGATTT 
GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054253.1
CDS	286..3960
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPDKLPRAHTCFNRLDLPPYESFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025198468.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198468.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCTTGCA GAAGATGGTG CAGTTGGGTC AGCAACAAAC 
AGTAACAACC ATCTCAGTGA ACCTCAGATA AGACGGCCTC GTAGCCTCAG TTCACCCACA 
GTAACTTTAT CTGCTCCACT TGAGGGACTC TCCAAGGACT CTCCTGCACG CCGAGCTGTA 
AAGGATACCC TTTCGAATCC TCAGTCTCCC CAGCCCTCAC CTTATAATTC TCCTAAACCA 
CAACACAAAG TGACACAGAG CTTCTTGCCC CCTGGCTGGG AGATGCGAAT AGCTCCAAAT 
GGCAGGCCCT TCTTCATTGA TCACAATACT AAAACCACTA CCTGGGAAGA TCCACGACTG 
AAATTTCCAG TACATTTGAG ATCAAAAGCT TCTTTGAACC CTAATGATTT GGGCCCTCTT 
CCTCCTGGTT GGGAGGAAAG AATACATTTG GATGGAAGAA CGTTTTATAT AGACCATAGA 
AGCCAGGTGT TGATATGTGG AGACATTGAT ACCAGAGACT TAGTGCCCTC ATACGATAAT 
AAAATTACCC AGTGGGAAGA TCCCAGATTG CAGAACCCAG CTATTACTGG TCCAGCTGTT 
CCCTATTCCA GAGAGTTCAA ACAGAAATAT GACTACTTCA GAAAGAAATT AAAGAAACCA 
GCTGATCTAC CAAACAGATT TGAGATGAAA CTCCACAGAA ATAATATTTT TGAGGAGTCC 
TATAGAAGAA TCATGTCCGT GAAGAGACCC GATGTATTAA AAGCCAGGCT CTGGATTGAA 
TTTGAAGCGG AAAAAGGACT TGATTATGGA GGTGTGGCCA GAGAATGGTT CTTTCTGTTG 
TCCAAGGAAA TGTTTAATCC TTATTATGGT CTTTTTGAAT ATTCGGCAAC GGACAACTAT 
ACTCTTCAGA TTAATCCAAA TTCAGGCCTT TGTAATGAAG ATCATCTGTC CTATTTCACA 
TTTATTGGCC GGGTTGCTGG GCTGGCAGTG TATCATGGGA AGTTATTAGA TGGCTTTTTC 
ATCAGGCCTT TTTATAAGAT GATGCTGGGG AAACCAATAA CTCTGAAAGA CATGGAATCA 
GTGGATAGTG AATATTACAA TTCCTTGAAA TGGATCCTGG AAAATGACCC AACAGAGCTG 
GATCTCATGT TTTGCATAGA TGAGGAAAAC TTTGGACAGA CTTATCAAGT GGACTTGAAG 
CCTAATGGAT CAGAAATCAT GGTAACAAAT GAGAACAAAC GGGAATATAT AGACTTAGTC 
ATCCAATGGA GGTTTGTAAA CAGAGTTCAG AAACAAATGA ATGCTTTTTT GGAGGGATTC 
ACAGAACTCC TACCTACTGA TTTGATTAAA ATTTTTGATG AAAATGAGCT GGAGTTACTA 
ATGTGTGGCC TTGGAGATGT TGATGTTAAT GACTGGAGAC AACATACGAT CTACAAGAAC 
GGATACTGTC CGAACCATCC TTTAATCCAG TGGTTCTGGA AGGCTGTGTT ACTGATGGAT 
GCTGAGAAAC GTATCCGGTT ACTGCAGTTT GTTACGGGGA CATCTCGAGT GCCTATGAAT 
GGATTTGCTG AACTTTACGG TTCAAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGATA AATTGCCCAG AGCTCACACA TGCTTTAATC GCCTTGACTT ACCTCCTTAT 
GAATCTTTTG AAGATTTACG AGAGAAACTT CTTATGGCAG TGGAAAATGC TCAAGGATTT 
GAAGGAGTGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025198469.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054254.1 Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

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CloneID	OAl83311
Clone ID Related Accession (Same CDS sequence)	XM_025198469.1
Accession Version	XM_025198469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841922.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054254.1
CDS	285..3884
Translation	MAHRLRFHFGSGRSNTAPESEILDREREDDFFMAFHTLPRRNSPHPFSRHGADYGGSDLQEVGTLKRSTSMFIPQLLNSIDVRPTRSSSMHISLQRKAVNGYADECGAPDSPDETLPCKFSDFRENLTSPMESRSSSPSSPEVTPENSPPRGPEELGQQVNGAASCNHSIFCTIPSSNQSDAAASTAPDDEASEAASGLNISGVRIQHRASSADMHQVTLLPFGSEAQHNVPASEPRRWSLQHLPDMSANSGKKLFVLQLQQPQASGTGIGGDYNFGFTGTKGDRLVRYPRIRLERSTSYPVQPRPEQISPVEDGANSELHGNGRNGSEISRSNSVERIPQGQGGCTFKIRPDQNTRQQHFRILVTRGPEEQNQAPAQESPGTPGPTAARATTRDDFLGQVDVPLNHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEDENNDQRDDSEHGWEVVDSNDSASHRQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPTLIDMASESDNNIRQINQEAAHRRFRSRRHISEDLEPEPMDSGDIPEPWETISEEVSASGDSLSLSLPPPPASPVSRTSPQELSDELNRRLQVTPDSNGEQLSSLIQRDPSSRLRSCSVTDAVAEQSQLSLQSGPSRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGAVGSATNSNNHLSEPQIRRPRSLSSPTVTLSAPLEGLSKDSPARRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHLRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHRSQVLICGDIDTRDLVPSYDNKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADLPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFEAEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVYHGKLLDGFFIRPFYKMMLGKPITLKDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPTDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNGYCPNHPLIQWFWKL

Target ORF information:

RefSeq Version	XM_025198469.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198469.1

ORF Insert Sequence:

ATGGCCCATC GGCTGCGGTT TCATTTTGGC TCTGGACGAA GCAACACAGC CCCTGAATCG 
GAGATCCTAG ACCGCGAAAG AGAAGATGAC TTTTTCATGG CATTCCATAC TTTGCCCCGA 
AGAAACAGTC CTCATCCTTT CTCCCGACAT GGAGCGGATT ATGGTGGCAG CGATTTACAA 
GAAGTGGGCA CCTTAAAAAG GAGCACGTCC ATGTTTATTC CTCAGCTGCT GAACAGTATT 
GATGTGCGCC CAACAAGGAG CTCATCTATG CACATTTCCC TTCAGCGCAA AGCGGTGAAT 
GGCTATGCTG ATGAGTGCGG AGCCCCAGAT TCTCCAGATG AGACGCTTCC ATGTAAATTC 
TCAGACTTTA GGGAGAATCT GACATCACCC ATGGAAAGTC GTTCTTCCTC GCCAAGCAGT 
CCAGAAGTGA CACCAGAAAA TTCACCGCCC AGGGGGCCTG AAGAACTAGG GCAACAGGTT 
AACGGAGCTG CTTCCTGTAA CCATTCAATA TTTTGTACAA TTCCTTCCAG TAACCAGAGT 
GACGCCGCTG CATCTACTGC CCCAGACGAT GAGGCAAGTG AAGCAGCTTC AGGTTTAAAC 
ATAAGTGGTG TACGGATTCA GCATCGTGCT TCCAGCGCCG ATATGCATCA GGTAACACTT 
TTGCCCTTTG GGTCTGAGGC TCAGCACAAT GTTCCTGCCT CTGAACCGAG GCGCTGGTCT 
TTGCAGCATC TGCCAGATAT GTCAGCGAAT TCAGGAAAAA AGCTCTTTGT TCTCCAGTTG 
CAGCAGCCAC AGGCCAGCGG TACGGGTATA GGAGGCGATT ACAATTTTGG TTTTACTGGA 
ACAAAAGGAG ACAGGTTGGT GAGATATCCA CGGATACGGC TGGAAAGGAG CACTTCCTAC 
CCGGTCCAGC CACGACCAGA ACAGATTAGC CCTGTGGAAG ATGGAGCAAA TTCAGAACTT 
CATGGAAATG GCAGAAATGG GTCAGAAATA TCTAGAAGCA ATTCAGTAGA AAGGATTCCT 
CAAGGACAAG GGGGATGCAC GTTTAAAATC AGACCAGATC AAAACACAAG GCAGCAACAT 
TTCAGAATTC TTGTAACCCG TGGTCCTGAA GAACAAAATC AAGCTCCTGC TCAGGAGAGC 
CCTGGTACAC CTGGTCCTAC AGCGGCCAGG GCCACAACTA GAGACGACTT CCTTGGTCAA 
GTAGACGTCC CACTTAATCA TCTTCCGACA GAAGACCCAA CCATGGAACG GCCATACACG 
TTTAAGGATT TTCTTCTCAG ACCAAGAAGC CACAAATCTC GGGTAAAGGG TTTCTTGAGA 
CTGAAGATGG CCTATATGCC GAAAAATGGA GGACAAGAGG ATGAAAATAA TGATCAAAGA 
GATGACTCGG AGCATGGGTG GGAAGTGGTT GATTCCAATG ATTCTGCATC TCATCGCCAA 
GAGGAGTTAC CGCCACCTCC ACTGCCTCCT GGATGGGAAG AAAAAGTGGA CAATCTTGGT 
CGTACTTACT ATGTCAACCA CAACAACAGG ACAACTCAGT GGCACCGGCC AACTTTAATT 
GATATGGCAT CTGAATCAGA CAACAACATT AGACAAATAA ACCAAGAGGC GGCTCATAGA 
CGCTTTCGAT CTCGGAGACA CATTAGTGAA GATTTGGAGC CAGAGCCCAT GGACAGTGGA 
GATATACCTG AACCCTGGGA AACTATTTCA GAGGAAGTGA GTGCAAGTGG AGATTCCTTA 
AGCCTGTCCT TGCCACCTCC CCCGGCCTCT CCAGTTTCTC GGACCAGTCC CCAGGAGCTG 
TCTGATGAAT TGAACAGAAG GCTCCAAGTC ACTCCTGATT CCAATGGGGA ACAACTCAGT 
TCTTTGATAC AAAGAGATCC TTCATCAAGA TTAAGGTCTT GCAGCGTAAC AGATGCAGTT 
GCTGAACAGT CTCAATTATC ACTGCAGAGT GGTCCATCTA GGAGAGCTCG TTCTTCAACT 
GTCACAGGTG GTGAAGAACC AACGCCATCA GTGGCCTATG TGCATACAAC ACCGGGCTTA 
CCTTCAGGCT GGGAAGAAAG GAAAGATGCT AAGGGACGTA CTTATTATGT CAATCATAAC 
AATCGAACCA CAACTTGGAC TCGGCCCATT ATGCAGCTTG CAGAAGATGG TGCAGTTGGG 
TCAGCAACAA ACAGTAACAA CCATCTCAGT GAACCTCAGA TAAGACGGCC TCGTAGCCTC 
AGTTCACCCA CAGTAACTTT ATCTGCTCCA CTTGAGGGAC TCTCCAAGGA CTCTCCTGCA 
CGCCGAGCTG TAAAGGATAC CCTTTCGAAT CCTCAGTCTC CCCAGCCCTC ACCTTATAAT 
TCTCCTAAAC CACAACACAA AGTGACACAG AGCTTCTTGC CCCCTGGCTG GGAGATGCGA 
ATAGCTCCAA ATGGCAGGCC CTTCTTCATT GATCACAATA CTAAAACCAC TACCTGGGAA 
GATCCACGAC TGAAATTTCC AGTACATTTG AGATCAAAAG CTTCTTTGAA CCCTAATGAT 
TTGGGCCCTC TTCCTCCTGG TTGGGAGGAA AGAATACATT TGGATGGAAG AACGTTTTAT 
ATAGACCATA GAAGCCAGGT GTTGATATGT GGAGACATTG ATACCAGAGA CTTAGTGCCC 
TCATACGATA ATAAAATTAC CCAGTGGGAA GATCCCAGAT TGCAGAACCC AGCTATTACT 
GGTCCAGCTG TTCCCTATTC CAGAGAGTTC AAACAGAAAT ATGACTACTT CAGAAAGAAA 
TTAAAGAAAC CAGCTGATCT ACCAAACAGA TTTGAGATGA AACTCCACAG AAATAATATT 
TTTGAGGAGT CCTATAGAAG AATCATGTCC GTGAAGAGAC CCGATGTATT AAAAGCCAGG 
CTCTGGATTG AATTTGAAGC GGAAAAAGGA CTTGATTATG GAGGTGTGGC CAGAGAATGG 
TTCTTTCTGT TGTCCAAGGA AATGTTTAAT CCTTATTATG GTCTTTTTGA ATATTCGGCA 
ACGGACAACT ATACTCTTCA GATTAATCCA AATTCAGGCC TTTGTAATGA AGATCATCTG 
TCCTATTTCA CATTTATTGG CCGGGTTGCT GGGCTGGCAG TGTATCATGG GAAGTTATTA 
GATGGCTTTT TCATCAGGCC TTTTTATAAG ATGATGCTGG GGAAACCAAT AACTCTGAAA 
GACATGGAAT CAGTGGATAG TGAATATTAC AATTCCTTGA AATGGATCCT GGAAAATGAC 
CCAACAGAGC TGGATCTCAT GTTTTGCATA GATGAGGAAA ACTTTGGACA GACTTATCAA 
GTGGACTTGA AGCCTAATGG ATCAGAAATC ATGGTAACAA ATGAGAACAA ACGGGAATAT 
ATAGACTTAG TCATCCAATG GAGGTTTGTA AACAGAGTTC AGAAACAAAT GAATGCTTTT 
TTGGAGGGAT TCACAGAACT CCTACCTACT GATTTGATTA AAATTTTTGA TGAAAATGAG 
CTGGAGTTAC TAATGTGTGG CCTTGGAGAT GTTGATGTTA ATGACTGGAG ACAACATACG 
ATCTACAAGA ACGGATACTG TCCGAACCAT CCTTTAATCC AGTGGTTCTG GAAGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEDD4L
Entrez Gene ID	102377323
Full Name	neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEDD4L cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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