AFAP1 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFAP1
Entrez Gene ID	102375340
Full Name	actin filament associated protein 1
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014527232.1	XP_014382718.1	actin filament-associated protein 1
XM_014527232.2	XP_014382718.1	actin filament-associated protein 1

Pathways from BioSystems
Gene Ontology

The following AFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl11924	XM_014527232.2 Latest version!	Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OAl11924	XM_014527232.1	Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFAP1 ( XM_014527232.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_014382718.1 Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.

Price & Availability↑

CloneID	OAl11924
Clone ID Related Accession (Same CDS sequence)	XM_014527232.1 , XM_014527232.2
Accession Version	XM_014527232.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	actin filament-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841871.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA GCTACGTCTG TTTCTTGAAC TTTTGGACCA TGAATATCTA 
ACATCCACTG TCAGAGAGAA AAAGGCAGTA ATAACTAACA TTCTCCTGAG GATACAGTCA 
TCAAAAGGTT TTGAAATAAA AGAACATGTA CAAAAACAAG AAGTCTCCAA TAGTTTACCT 
GCCCCTCCTC AGATGCCTCT GCCAGAGATA CCTCAGCAAT GGCTGCCCCC AGATAATGGG 
CCTCCACCAT TACCAACATC ATCCCTTCCA GAAGGCTACT ATGAAGAGGC AGTGCCACTT 
AGTCCTGGAA AGGCCCCCGA ATACATCACT TCCAATTATG ACTCGGATGC CATGAGCAGC 
TCTTATGAAT CATATGATGA AGAGGAGGAG GATGGGAAGG GGAAAAAAAT GAGACATCAA 
TGGCCTTCTG AGGAGGCCTC TATGGACCTA GTGAAGGATG CTAAGATCTG TGCCTTCCTC 
CTAAGAAAGA AGCGTTTTGG ACAATGGACC AAATTACTGT GTGTTATCAA AGAAAACAAA 
CTACTGTGTT ATAAAAGTTC GAAGGATCAG CAACCTCAGA TGGAACTGCT CTTGAATGAC 
TGCAGCATCA CATATATTCC CAAAGACAGC AAAAAGAAGA AGCATGAGCT GAAAATCAGT 
CATCAAGGAA CAGATGCACT GGTTCTAGCA GTACAAAGCA AAGAGCAGGC AGAGCAGTGG 
TTGAAGGTAA TAAAAGAAGT TTGCAACAAC TGTACAGGAA CTATAGACTC AGATGGACCA 
TCATCTAGCT CACCAGTACA CAAGACAGAA CTGGAAAAGA AATTGTCTTC TGAGAGACCA 
AGTTCAGATG GGGAGGGTGC TATAGAAAAT GGAATCACTG CTGTGTGTAA TGGAAAAGAA 
CAAGTGAAGA GGAAAAAGAG TTCAAAGTCA GAGGCCAAGG GTACTGTAAC TAAAGTAACA 
GGGAAAAAAA TAACGAAGAT CATTGGTTTA GGAAAGAAAA AACCATCAAC AGATGAACAG 
ACCTCATCGG CTGAGGAAGA TGTTCCAACA TGTGGGTATC TCAATGTGCT CTCAAATAAC 
CGCTGGCGTG AGCGCTGGTG CAGAGTGAAA GATAATAAGC TCATTTTCCA CAAGGACAGG 
ACAGATCTGA AGACCCATAT TGTTTCTATC CCCCTACGTG GTTGTGAAGT CATTCCAGGA 
TTGGATTCCA AGCATCCCTT AACGTTCCGT CTGCTTCGAA ATGGGCAGGA AGTTGCAGTA 
TTAGAGGCCT CATCATCTGA AGACATGGGC AGATGGATTG GAATTCTGTT AGCTGAAACT 
GGTTCATCTA CAGACCCTGG AGCTCTGCAC TATGACTACA TTGATGTGGA GATGACTGCA 
AGTGTCATAC AAGCTGCAAA GCAGACCTTC TGCTTTATGA ACAGACGTGT TATAACTACT 
AATCCATACC GGGGAAGCAC TGCCAATGGC TACGCATGTC CCAGTGGAAT GGCACTTCAT 
TACGATGATG TTCCCTGCAT AAATGGATCG TGGGAACCAG AAGATGGCTT TCCTACTTCT 
CGTAACAGAA ACATCGGAGA AGAAATGCTT TATGATAACG CAGGCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC ACGCTATCCA CCAGCTGACA GAAAAGCCAA TAGGTTATCT 
ACTGACAAAC TATCCTCTAA CCATTACAAA TACCCTGTTT CATCCAGTCT GTCTTCTGCT 
CAGTCTATCA CTAACACCTC TGCTGTGGGG AGGGGATCTG TCTCTCAGTT CAAAAGCAAG 
AAACCCCCTG CAACTGCAAA TGGTGTCACA GGAAAAGGGA GAGTTTTAAA CAACCAACCA 
AAGAAATCTG AATTAGTTTC ATGTGTGAAA CGTACAGCAT CTAATGCTGA TCAATACAAA 
TATGGCAAGA ACCGGGTGGA AGCAGATGCA AAGAGGTTAC AAAGTAAGGA GGAGGAGTTA 
TTAAAGAGGA AAGAAGCTCT GCGGAATAGG TTGGCCCAAC TCCGAAAAGA AAGGAAAGAC 
TTACGAGCTG CCATTGAAGT CAATGCTGGC AGGAAAACCC AAGTGATTCT TGAAGATAAG 
TTAAAGAAGT TGGAAGAGGA ATGTAAGCTG AAGGAGAGTG AACGTGTCAA TTTGGAGTTA 
GAACTGACTG AGGTGAAAGA GAGCTTGAAG AAAGCCTTGG CTGGAGGCAT TACACTAGGA 
TTGGCCATTG AGCCCAAATC AGGAACCTCA AGCCCACAGT CTCCAATATT CAAGCACCGA 
ACTATGGAAA ACTCCCCTAT CTCCAGTTGT GATACAAGTG ATACTGAATG CTCAGTACCT 
GTGAATAGTG CAGCAGCTCT GAAGAGACCT TCCTCATCAA GTAATTCTCC TTGTCGAGGC 
CATGTACTTA GAAAAGCAAA GGAATGGGAG ATGAAAAATG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014382718.1
CDS	1..2448
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFEIKEHVQKQEVSNSLPAPPQMPLPEIPQQWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKMRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKENKLLCYKSSKDQQPQMELLLNDCSITYIPKDSKKKKHELKISHQGTDALVLAVQSKEQAEQWLKVIKEVCNNCTGTIDSDGPSSSSPVHKTELEKKLSSERPSSDGEGAIENGITAVCNGKEQVKRKKSSKSEAKGTVTKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMTASVIQAAKQTFCFMNRRVITTNPYRGSTANGYACPSGMALHYDDVPCINGSWEPEDGFPTSRNRNIGEEMLYDNAGLYDNLPSPKIFARYPPADRKANRLSTDKLSSNHYKYPVSSSLSSAQSITNTSAVGRGSVSQFKSKKPPATANGVTGKGRVLNNQPKKSELVSCVKRTASNADQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVILEDKLKKLEEECKLKESERVNLELELTEVKESLKKALAGGITLGLAIEPKSGTSSPQSPIFKHRTMENSPISSCDTSDTECSVPVNSAAALKRPSSSSNSPCRGHVLRKAKEWEMKNGT

Target ORF information:

RefSeq Version	XM_014527232.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014527232.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA GCTACGTCTG TTTCTTGAAC TTTTGGACCA TGAATATCTA 
ACATCCACTG TCAGAGAGAA AAAGGCAGTA ATAACTAACA TTCTCCTGAG GATACAGTCA 
TCAAAAGGTT TTGAAATAAA AGAACATGTA CAAAAACAAG AAGTCTCCAA TAGTTTACCT 
GCCCCTCCTC AGATGCCTCT GCCAGAGATA CCTCAGCAAT GGCTGCCCCC AGATAATGGG 
CCTCCACCAT TACCAACATC ATCCCTTCCA GAAGGCTACT ATGAAGAGGC AGTGCCACTT 
AGTCCTGGAA AGGCCCCCGA ATACATCACT TCCAATTATG ACTCGGATGC CATGAGCAGC 
TCTTATGAAT CATATGATGA AGAGGAGGAG GATGGGAAGG GGAAAAAAAT GAGACATCAA 
TGGCCTTCTG AGGAGGCCTC TATGGACCTA GTGAAGGATG CTAAGATCTG TGCCTTCCTC 
CTAAGAAAGA AGCGTTTTGG ACAATGGACC AAATTACTGT GTGTTATCAA AGAAAACAAA 
CTACTGTGTT ATAAAAGTTC GAAGGATCAG CAACCTCAGA TGGAACTGCT CTTGAATGAC 
TGCAGCATCA CATATATTCC CAAAGACAGC AAAAAGAAGA AGCATGAGCT GAAAATCAGT 
CATCAAGGAA CAGATGCACT GGTTCTAGCA GTACAAAGCA AAGAGCAGGC AGAGCAGTGG 
TTGAAGGTAA TAAAAGAAGT TTGCAACAAC TGTACAGGAA CTATAGACTC AGATGGACCA 
TCATCTAGCT CACCAGTACA CAAGACAGAA CTGGAAAAGA AATTGTCTTC TGAGAGACCA 
AGTTCAGATG GGGAGGGTGC TATAGAAAAT GGAATCACTG CTGTGTGTAA TGGAAAAGAA 
CAAGTGAAGA GGAAAAAGAG TTCAAAGTCA GAGGCCAAGG GTACTGTAAC TAAAGTAACA 
GGGAAAAAAA TAACGAAGAT CATTGGTTTA GGAAAGAAAA AACCATCAAC AGATGAACAG 
ACCTCATCGG CTGAGGAAGA TGTTCCAACA TGTGGGTATC TCAATGTGCT CTCAAATAAC 
CGCTGGCGTG AGCGCTGGTG CAGAGTGAAA GATAATAAGC TCATTTTCCA CAAGGACAGG 
ACAGATCTGA AGACCCATAT TGTTTCTATC CCCCTACGTG GTTGTGAAGT CATTCCAGGA 
TTGGATTCCA AGCATCCCTT AACGTTCCGT CTGCTTCGAA ATGGGCAGGA AGTTGCAGTA 
TTAGAGGCCT CATCATCTGA AGACATGGGC AGATGGATTG GAATTCTGTT AGCTGAAACT 
GGTTCATCTA CAGACCCTGG AGCTCTGCAC TATGACTACA TTGATGTGGA GATGACTGCA 
AGTGTCATAC AAGCTGCAAA GCAGACCTTC TGCTTTATGA ACAGACGTGT TATAACTACT 
AATCCATACC GGGGAAGCAC TGCCAATGGC TACGCATGTC CCAGTGGAAT GGCACTTCAT 
TACGATGATG TTCCCTGCAT AAATGGATCG TGGGAACCAG AAGATGGCTT TCCTACTTCT 
CGTAACAGAA ACATCGGAGA AGAAATGCTT TATGATAACG CAGGCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC ACGCTATCCA CCAGCTGACA GAAAAGCCAA TAGGTTATCT 
ACTGACAAAC TATCCTCTAA CCATTACAAA TACCCTGTTT CATCCAGTCT GTCTTCTGCT 
CAGTCTATCA CTAACACCTC TGCTGTGGGG AGGGGATCTG TCTCTCAGTT CAAAAGCAAG 
AAACCCCCTG CAACTGCAAA TGGTGTCACA GGAAAAGGGA GAGTTTTAAA CAACCAACCA 
AAGAAATCTG AATTAGTTTC ATGTGTGAAA CGTACAGCAT CTAATGCTGA TCAATACAAA 
TATGGCAAGA ACCGGGTGGA AGCAGATGCA AAGAGGTTAC AAAGTAAGGA GGAGGAGTTA 
TTAAAGAGGA AAGAAGCTCT GCGGAATAGG TTGGCCCAAC TCCGAAAAGA AAGGAAAGAC 
TTACGAGCTG CCATTGAAGT CAATGCTGGC AGGAAAACCC AAGTGATTCT TGAAGATAAG 
TTAAAGAAGT TGGAAGAGGA ATGTAAGCTG AAGGAGAGTG AACGTGTCAA TTTGGAGTTA 
GAACTGACTG AGGTGAAAGA GAGCTTGAAG AAAGCCTTGG CTGGAGGCAT TACACTAGGA 
TTGGCCATTG AGCCCAAATC AGGAACCTCA AGCCCACAGT CTCCAATATT CAAGCACCGA 
ACTATGGAAA ACTCCCCTAT CTCCAGTTGT GATACAAGTG ATACTGAATG CTCAGTACCT 
GTGAATAGTG CAGCAGCTCT GAAGAGACCT TCCTCATCAA GTAATTCTCC TTGTCGAGGC 
CATGTACTTA GAAAAGCAAA GGAATGGGAG ATGAAAAATG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1 ( XM_014527232.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_014382718.1 Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.

Price & Availability↑

CloneID	OAl11924
Clone ID Related Accession (Same CDS sequence)	XM_014527232.1 , XM_014527232.2
Accession Version	XM_014527232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	actin filament-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841871.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014527232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA GCTACGTCTG TTTCTTGAAC TTTTGGACCA TGAATATCTA 
ACATCCACTG TCAGAGAGAA AAAGGCAGTA ATAACTAACA TTCTCCTGAG GATACAGTCA 
TCAAAAGGTT TTGAAATAAA AGAACATGTA CAAAAACAAG AAGTCTCCAA TAGTTTACCT 
GCCCCTCCTC AGATGCCTCT GCCAGAGATA CCTCAGCAAT GGCTGCCCCC AGATAATGGG 
CCTCCACCAT TACCAACATC ATCCCTTCCA GAAGGCTACT ATGAAGAGGC AGTGCCACTT 
AGTCCTGGAA AGGCCCCCGA ATACATCACT TCCAATTATG ACTCGGATGC CATGAGCAGC 
TCTTATGAAT CATATGATGA AGAGGAGGAG GATGGGAAGG GGAAAAAAAT GAGACATCAA 
TGGCCTTCTG AGGAGGCCTC TATGGACCTA GTGAAGGATG CTAAGATCTG TGCCTTCCTC 
CTAAGAAAGA AGCGTTTTGG ACAATGGACC AAATTACTGT GTGTTATCAA AGAAAACAAA 
CTACTGTGTT ATAAAAGTTC GAAGGATCAG CAACCTCAGA TGGAACTGCT CTTGAATGAC 
TGCAGCATCA CATATATTCC CAAAGACAGC AAAAAGAAGA AGCATGAGCT GAAAATCAGT 
CATCAAGGAA CAGATGCACT GGTTCTAGCA GTACAAAGCA AAGAGCAGGC AGAGCAGTGG 
TTGAAGGTAA TAAAAGAAGT TTGCAACAAC TGTACAGGAA CTATAGACTC AGATGGACCA 
TCATCTAGCT CACCAGTACA CAAGACAGAA CTGGAAAAGA AATTGTCTTC TGAGAGACCA 
AGTTCAGATG GGGAGGGTGC TATAGAAAAT GGAATCACTG CTGTGTGTAA TGGAAAAGAA 
CAAGTGAAGA GGAAAAAGAG TTCAAAGTCA GAGGCCAAGG GTACTGTAAC TAAAGTAACA 
GGGAAAAAAA TAACGAAGAT CATTGGTTTA GGAAAGAAAA AACCATCAAC AGATGAACAG 
ACCTCATCGG CTGAGGAAGA TGTTCCAACA TGTGGGTATC TCAATGTGCT CTCAAATAAC 
CGCTGGCGTG AGCGCTGGTG CAGAGTGAAA GATAATAAGC TCATTTTCCA CAAGGACAGG 
ACAGATCTGA AGACCCATAT TGTTTCTATC CCCCTACGTG GTTGTGAAGT CATTCCAGGA 
TTGGATTCCA AGCATCCCTT AACGTTCCGT CTGCTTCGAA ATGGGCAGGA AGTTGCAGTA 
TTAGAGGCCT CATCATCTGA AGACATGGGC AGATGGATTG GAATTCTGTT AGCTGAAACT 
GGTTCATCTA CAGACCCTGG AGCTCTGCAC TATGACTACA TTGATGTGGA GATGACTGCA 
AGTGTCATAC AAGCTGCAAA GCAGACCTTC TGCTTTATGA ACAGACGTGT TATAACTACT 
AATCCATACC GGGGAAGCAC TGCCAATGGC TACGCATGTC CCAGTGGAAT GGCACTTCAT 
TACGATGATG TTCCCTGCAT AAATGGATCG TGGGAACCAG AAGATGGCTT TCCTACTTCT 
CGTAACAGAA ACATCGGAGA AGAAATGCTT TATGATAACG CAGGCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC ACGCTATCCA CCAGCTGACA GAAAAGCCAA TAGGTTATCT 
ACTGACAAAC TATCCTCTAA CCATTACAAA TACCCTGTTT CATCCAGTCT GTCTTCTGCT 
CAGTCTATCA CTAACACCTC TGCTGTGGGG AGGGGATCTG TCTCTCAGTT CAAAAGCAAG 
AAACCCCCTG CAACTGCAAA TGGTGTCACA GGAAAAGGGA GAGTTTTAAA CAACCAACCA 
AAGAAATCTG AATTAGTTTC ATGTGTGAAA CGTACAGCAT CTAATGCTGA TCAATACAAA 
TATGGCAAGA ACCGGGTGGA AGCAGATGCA AAGAGGTTAC AAAGTAAGGA GGAGGAGTTA 
TTAAAGAGGA AAGAAGCTCT GCGGAATAGG TTGGCCCAAC TCCGAAAAGA AAGGAAAGAC 
TTACGAGCTG CCATTGAAGT CAATGCTGGC AGGAAAACCC AAGTGATTCT TGAAGATAAG 
TTAAAGAAGT TGGAAGAGGA ATGTAAGCTG AAGGAGAGTG AACGTGTCAA TTTGGAGTTA 
GAACTGACTG AGGTGAAAGA GAGCTTGAAG AAAGCCTTGG CTGGAGGCAT TACACTAGGA 
TTGGCCATTG AGCCCAAATC AGGAACCTCA AGCCCACAGT CTCCAATATT CAAGCACCGA 
ACTATGGAAA ACTCCCCTAT CTCCAGTTGT GATACAAGTG ATACTGAATG CTCAGTACCT 
GTGAATAGTG CAGCAGCTCT GAAGAGACCT TCCTCATCAA GTAATTCTCC TTGTCGAGGC 
CATGTACTTA GAAAAGCAAA GGAATGGGAG ATGAAAAATG GAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014382718.1
CDS	58..2505
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFEIKEHVQKQEVSNSLPAPPQMPLPEIPQQWLPPDNGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKMRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKENKLLCYKSSKDQQPQMELLLNDCSITYIPKDSKKKKHELKISHQGTDALVLAVQSKEQAEQWLKVIKEVCNNCTGTIDSDGPSSSSPVHKTELEKKLSSERPSSDGEGAIENGITAVCNGKEQVKRKKSSKSEAKGTVTKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMTASVIQAAKQTFCFMNRRVITTNPYRGSTANGYACPSGMALHYDDVPCINGSWEPEDGFPTSRNRNIGEEMLYDNAGLYDNLPSPKIFARYPPADRKANRLSTDKLSSNHYKYPVSSSLSSAQSITNTSAVGRGSVSQFKSKKPPATANGVTGKGRVLNNQPKKSELVSCVKRTASNADQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVILEDKLKKLEEECKLKESERVNLELELTEVKESLKKALAGGITLGLAIEPKSGTSSPQSPIFKHRTMENSPISSCDTSDTECSVPVNSAAALKRPSSSSNSPCRGHVLRKAKEWEMKNGT

Target ORF information:

RefSeq Version	XM_014527232.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis actin filament associated protein 1 (AFAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014527232.2

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA GCTACGTCTG TTTCTTGAAC TTTTGGACCA TGAATATCTA 
ACATCCACTG TCAGAGAGAA AAAGGCAGTA ATAACTAACA TTCTCCTGAG GATACAGTCA 
TCAAAAGGTT TTGAAATAAA AGAACATGTA CAAAAACAAG AAGTCTCCAA TAGTTTACCT 
GCCCCTCCTC AGATGCCTCT GCCAGAGATA CCTCAGCAAT GGCTGCCCCC AGATAATGGG 
CCTCCACCAT TACCAACATC ATCCCTTCCA GAAGGCTACT ATGAAGAGGC AGTGCCACTT 
AGTCCTGGAA AGGCCCCCGA ATACATCACT TCCAATTATG ACTCGGATGC CATGAGCAGC 
TCTTATGAAT CATATGATGA AGAGGAGGAG GATGGGAAGG GGAAAAAAAT GAGACATCAA 
TGGCCTTCTG AGGAGGCCTC TATGGACCTA GTGAAGGATG CTAAGATCTG TGCCTTCCTC 
CTAAGAAAGA AGCGTTTTGG ACAATGGACC AAATTACTGT GTGTTATCAA AGAAAACAAA 
CTACTGTGTT ATAAAAGTTC GAAGGATCAG CAACCTCAGA TGGAACTGCT CTTGAATGAC 
TGCAGCATCA CATATATTCC CAAAGACAGC AAAAAGAAGA AGCATGAGCT GAAAATCAGT 
CATCAAGGAA CAGATGCACT GGTTCTAGCA GTACAAAGCA AAGAGCAGGC AGAGCAGTGG 
TTGAAGGTAA TAAAAGAAGT TTGCAACAAC TGTACAGGAA CTATAGACTC AGATGGACCA 
TCATCTAGCT CACCAGTACA CAAGACAGAA CTGGAAAAGA AATTGTCTTC TGAGAGACCA 
AGTTCAGATG GGGAGGGTGC TATAGAAAAT GGAATCACTG CTGTGTGTAA TGGAAAAGAA 
CAAGTGAAGA GGAAAAAGAG TTCAAAGTCA GAGGCCAAGG GTACTGTAAC TAAAGTAACA 
GGGAAAAAAA TAACGAAGAT CATTGGTTTA GGAAAGAAAA AACCATCAAC AGATGAACAG 
ACCTCATCGG CTGAGGAAGA TGTTCCAACA TGTGGGTATC TCAATGTGCT CTCAAATAAC 
CGCTGGCGTG AGCGCTGGTG CAGAGTGAAA GATAATAAGC TCATTTTCCA CAAGGACAGG 
ACAGATCTGA AGACCCATAT TGTTTCTATC CCCCTACGTG GTTGTGAAGT CATTCCAGGA 
TTGGATTCCA AGCATCCCTT AACGTTCCGT CTGCTTCGAA ATGGGCAGGA AGTTGCAGTA 
TTAGAGGCCT CATCATCTGA AGACATGGGC AGATGGATTG GAATTCTGTT AGCTGAAACT 
GGTTCATCTA CAGACCCTGG AGCTCTGCAC TATGACTACA TTGATGTGGA GATGACTGCA 
AGTGTCATAC AAGCTGCAAA GCAGACCTTC TGCTTTATGA ACAGACGTGT TATAACTACT 
AATCCATACC GGGGAAGCAC TGCCAATGGC TACGCATGTC CCAGTGGAAT GGCACTTCAT 
TACGATGATG TTCCCTGCAT AAATGGATCG TGGGAACCAG AAGATGGCTT TCCTACTTCT 
CGTAACAGAA ACATCGGAGA AGAAATGCTT TATGATAACG CAGGCCTGTA CGATAACTTG 
CCGTCTCCGA AAATCTTTGC ACGCTATCCA CCAGCTGACA GAAAAGCCAA TAGGTTATCT 
ACTGACAAAC TATCCTCTAA CCATTACAAA TACCCTGTTT CATCCAGTCT GTCTTCTGCT 
CAGTCTATCA CTAACACCTC TGCTGTGGGG AGGGGATCTG TCTCTCAGTT CAAAAGCAAG 
AAACCCCCTG CAACTGCAAA TGGTGTCACA GGAAAAGGGA GAGTTTTAAA CAACCAACCA 
AAGAAATCTG AATTAGTTTC ATGTGTGAAA CGTACAGCAT CTAATGCTGA TCAATACAAA 
TATGGCAAGA ACCGGGTGGA AGCAGATGCA AAGAGGTTAC AAAGTAAGGA GGAGGAGTTA 
TTAAAGAGGA AAGAAGCTCT GCGGAATAGG TTGGCCCAAC TCCGAAAAGA AAGGAAAGAC 
TTACGAGCTG CCATTGAAGT CAATGCTGGC AGGAAAACCC AAGTGATTCT TGAAGATAAG 
TTAAAGAAGT TGGAAGAGGA ATGTAAGCTG AAGGAGAGTG AACGTGTCAA TTTGGAGTTA 
GAACTGACTG AGGTGAAAGA GAGCTTGAAG AAAGCCTTGG CTGGAGGCAT TACACTAGGA 
TTGGCCATTG AGCCCAAATC AGGAACCTCA AGCCCACAGT CTCCAATATT CAAGCACCGA 
ACTATGGAAA ACTCCCCTAT CTCCAGTTGT GATACAAGTG ATACTGAATG CTCAGTACCT 
GTGAATAGTG CAGCAGCTCT GAAGAGACCT TCCTCATCAA GTAATTCTCC TTGTCGAGGC 
CATGTACTTA GAAAAGCAAA GGAATGGGAG ATGAAAAATG GAACCTAA