MIEF2 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIEF2
Entrez Gene ID	102371131
Full Name	mitochondrial elongation factor 2
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006024055.1	XP_006024117.1	mitochondrial dynamics protein MID49

Pathways from BioSystems
Gene Ontology

The following MIEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl18599	XM_006024055.1 Latest version!	Alligator sinensis mitochondrial elongation factor 2 (MIEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIEF2 ( XM_006024055.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006024117.1 Alligator sinensis mitochondrial elongation factor 2 (MIEF2), mRNA.

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CloneID	OAl18599
Clone ID Related Accession (Same CDS sequence)	XM_006024055.1
Accession Version	XM_006024055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	mitochondrial dynamics protein MID49
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842091.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGCTGAGT TTATACACAA GCAGGGGAAG AGGCAGGACG ACCGTGGGCT GGGCAGTGTC 
ATCGACTTCC TCTTGGCCAA CACCAGGCTG GTGCTGGGGG TGAGCGGTGC TGCTGTGCTG 
GCCATTGCGA CTCTGGTGGT GAAACGGATG ATCGACCGAG CCACCAGCTC GCCTGACGAG 
GACGATGCCA AAGCAGAGCA GAAGTCCATT GAGGAGAGCT GGCAGGATCT GAATTTGCTC 
AAGGCACCGC AGAAGCCTCC CAAGAAGCCA AGCAGGGAGG ACCTCAGCGA GCCTCTGCTG 
CCTCTGCCTG TAACCCAGCC TGCCCAAGAG CTATGCAAAC ATCCTGCCTC CCTGGAGACT 
CTCTGCATAG AATCCAGACC TCTGCTTTGC CTCACGTTGC AGGAGAAGCT CCTCTCTTAC 
TATAACAACC ACGTCGCCAT CCCAGAGCCG GAAGCAGCCC TGGGGAGGCA GCTTGCAAAG 
GATGTCTGTG TGGAACTGCA GAATTTCCTG CAAAACAAGT ACCCAGAACT GCCTTTCGGC 
AGGATGGTTC TCAGCGGTTC CCTCCACAAT GACCTCCAGG TGGTTGCTGC CGACCATGTT 
GACTTCCTGC TGCCTATGGC ACTTGAAGCT GCGCTCTGGA AGCTGATCCC TGGGGAGGAC 
ACTGTTGTGA AAACCCCCCA GTTCTGGATG ATTAGACGGA CCAACTTGGA GTACTTCCCG 
AGGGGGAGCA GTCCCTGGGA CCGATTCCTG GTGGGTGGGT ACCTCTCGTC CAATGCGCTC 
AGTGAGACGA TCCATAAAAT ACTGGAGACC TCCATCAACT GGCCTGCCAT AGGCAGCATG 
CTGGAGTGCA TCATCAGACC GACGGTGGCT CCTGAGGACC TGAAGCTGGA GGTCGAGCAT 
TGTCAGGTTC AGTTGAATGT AGCCCTCTAT CCAGTGGTCA AGGTGGAGGA CAAGGTTCTT 
CTGGCCAAGC CTCCCAGGGG ACTTGTGGAA AACCTCTGGC AACAAAGCTT TTATGCAGCT 
GAGGTCTCCA GACTCAAAGA TCTGGATGCT GGAGACTCGG GGGTCAGGCA GCTCTGCCTC 
AAAATCTGGA AGGCGGTCTG CAGAGACCAC CCCTCCTTGG GCAGACTGAC TGGCAGTCAC 
TTGACTCACG TCCTCCTCCA CCTCAGTGAA ATGGAGCTGG ACTGGGCAGA GGCCACCTTG 
GCCGCTAGGT TCCAGCAAAT GATCGAGGAG CTGGTGGGCT ACCTGGAGAA AGGCTTTCTC 
CCTTGCTATT TCAACAGCAG AATCAACCTG TTCCATGAGC TGCTGGAAGA GGAAATCGAT 
GAGATGGGGA CAGGGCTCTC GCTCCCAGAT TTGGGCCCCA ATTCTGTGCT GCGTCAGAAC 
ACACCGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006024117.1
CDS	82..1473
Translation	MAEFIHKQGKRQDDRGLGSVIDFLLANTRLVLGVSGAAVLAIATLVVKRMIDRATSSPDEDDAKAEQKSIEESWQDLNLLKAPQKPPKKPSREDLSEPLLPLPVTQPAQELCKHPASLETLCIESRPLLCLTLQEKLLSYYNNHVAIPEPEAALGRQLAKDVCVELQNFLQNKYPELPFGRMVLSGSLHNDLQVVAADHVDFLLPMALEAALWKLIPGEDTVVKTPQFWMIRRTNLEYFPRGSSPWDRFLVGGYLSSNALSETIHKILETSINWPAIGSMLECIIRPTVAPEDLKLEVEHCQVQLNVALYPVVKVEDKVLLAKPPRGLVENLWQQSFYAAEVSRLKDLDAGDSGVRQLCLKIWKAVCRDHPSLGRLTGSHLTHVLLHLSEMELDWAEATLAARFQQMIEELVGYLEKGFLPCYFNSRINLFHELLEEEIDEMGTGLSLPDLGPNSVLRQNTPS

Target ORF information:

RefSeq Version	XM_006024055.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis mitochondrial elongation factor 2 (MIEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006024055.1

ORF Insert Sequence:

ATGGCTGAGT TTATACACAA GCAGGGGAAG AGGCAGGACG ACCGTGGGCT GGGCAGTGTC 
ATCGACTTCC TCTTGGCCAA CACCAGGCTG GTGCTGGGGG TGAGCGGTGC TGCTGTGCTG 
GCCATTGCGA CTCTGGTGGT GAAACGGATG ATCGACCGAG CCACCAGCTC GCCTGACGAG 
GACGATGCCA AAGCAGAGCA GAAGTCCATT GAGGAGAGCT GGCAGGATCT GAATTTGCTC 
AAGGCACCGC AGAAGCCTCC CAAGAAGCCA AGCAGGGAGG ACCTCAGCGA GCCTCTGCTG 
CCTCTGCCTG TAACCCAGCC TGCCCAAGAG CTATGCAAAC ATCCTGCCTC CCTGGAGACT 
CTCTGCATAG AATCCAGACC TCTGCTTTGC CTCACGTTGC AGGAGAAGCT CCTCTCTTAC 
TATAACAACC ACGTCGCCAT CCCAGAGCCG GAAGCAGCCC TGGGGAGGCA GCTTGCAAAG 
GATGTCTGTG TGGAACTGCA GAATTTCCTG CAAAACAAGT ACCCAGAACT GCCTTTCGGC 
AGGATGGTTC TCAGCGGTTC CCTCCACAAT GACCTCCAGG TGGTTGCTGC CGACCATGTT 
GACTTCCTGC TGCCTATGGC ACTTGAAGCT GCGCTCTGGA AGCTGATCCC TGGGGAGGAC 
ACTGTTGTGA AAACCCCCCA GTTCTGGATG ATTAGACGGA CCAACTTGGA GTACTTCCCG 
AGGGGGAGCA GTCCCTGGGA CCGATTCCTG GTGGGTGGGT ACCTCTCGTC CAATGCGCTC 
AGTGAGACGA TCCATAAAAT ACTGGAGACC TCCATCAACT GGCCTGCCAT AGGCAGCATG 
CTGGAGTGCA TCATCAGACC GACGGTGGCT CCTGAGGACC TGAAGCTGGA GGTCGAGCAT 
TGTCAGGTTC AGTTGAATGT AGCCCTCTAT CCAGTGGTCA AGGTGGAGGA CAAGGTTCTT 
CTGGCCAAGC CTCCCAGGGG ACTTGTGGAA AACCTCTGGC AACAAAGCTT TTATGCAGCT 
GAGGTCTCCA GACTCAAAGA TCTGGATGCT GGAGACTCGG GGGTCAGGCA GCTCTGCCTC 
AAAATCTGGA AGGCGGTCTG CAGAGACCAC CCCTCCTTGG GCAGACTGAC TGGCAGTCAC 
TTGACTCACG TCCTCCTCCA CCTCAGTGAA ATGGAGCTGG ACTGGGCAGA GGCCACCTTG 
GCCGCTAGGT TCCAGCAAAT GATCGAGGAG CTGGTGGGCT ACCTGGAGAA AGGCTTTCTC 
CCTTGCTATT TCAACAGCAG AATCAACCTG TTCCATGAGC TGCTGGAAGA GGAAATCGAT 
GAGATGGGGA CAGGGCTCTC GCTCCCAGAT TTGGGCCCCA ATTCTGTGCT GCGTCAGAAC 
ACACCGTCTT GA