LOC110989330 cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110989330
Entrez Gene ID	110989330
Full Name	stonustoxin subunit beta-like
Gene Type	protein-coding
Organism	Acanthaster planci(crown-of-thorns starfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022253637.1	XP_022109329.1	stonustoxin subunit beta-like
XM_022253638.1	XP_022109330.1	stonustoxin subunit beta-like

Pathways from BioSystems
Gene Ontology

The following LOC110989330 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110989330 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc172521	XM_022253638.1 Latest version!	Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAc172521	XM_022253637.1 Latest version!	Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110989330 ( XM_022253637.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022109329.1 Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc172521
Clone ID Related Accession (Same CDS sequence)	XM_022253637.1 , XM_022253638.1
Accession Version	XM_022253637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	stonustoxin subunit beta-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091638.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC AAGGCGTGTT AGAGCTGGCT ACCTTAGGGC GTCCCATGCA GCTGGGTATG 
CTTTACGATT GCCGTAGAGA CAGTCTGATT CCTGGATTAA CTCTCTGGGA TCCAGCTGTT 
TTGCAGAATG ATCGTGACGT GCGTCCTCAG CCCAGCACAC AGTACGACAT CATGTCATCT 
GACAGAGTGG ACGACAAGGC ATCCTCTCTC AACATCAGCG CGTCGTTGAG GGCTAGCTTC 
TTGGGTGGAC TCATAGAGGT CAAAGGCTCG GCCAAGTTCC TTCACGACAA AAAGTCTTCT 
ATTAAACAAG CTCGCCTTAC ACTGCATTAC AAAACTACCA CAAGGTTTGA GCAGTTGACA 
ATGAAGCATC TAGGTGCACA CAATGTTCAG CATTCTGACA TATTTGACCA AGGTACAGCT 
ACTCATGTTG TAACAGGTAT CCTCTATGGG GCCCATGCAT TTTTCGTATT CGATAGGGAC 
TGTACGAGAG AGGAAGAAAT CAAAGATGTG CAGGGGCGAA TGCATGGCAT GGTGAAAAGA 
ATAGCTTCAT TTGAAGCGGG TGGGGAGGGG TCTCTAGAGG AGGGTGGACG AGAGGGTGTT 
GATAAGATGC GCTGTAGTTT CCACGGTGAC TTTAACCTAG AAAATAACCC GGCAAGTTAC 
CAAGACGCTG TCAGCGTCTA TACGAAACTG CCCTTAATGC TTGGCAGGGA CGGGGAATAT 
GCGGTTCCCA TACGGGCTTG GCTATATCCT TTAGATAAGT TGGATCAAAA AGCTGCCAAG 
TTGACTCGTC AAATAAGTGT CAACTTAATA AGTCAATCCC AGGAGATTTT GGAAGGCTTG 
GAGGACGTGG GGGTTAAATG CGACGACATG ATTATGTCGG ACATTTGCAG AGATTTCCCC 
CAGTTTCGGG AGAAGTTCGG TCCGTTTAAA CAGAAGATCG TCCAGTATAA ATTGTATTTA 
CAAGAGTATC TGGTGCAAGC ATTGCCGGCA ATCCGAGGGG GCAGTGTTGA GGAGAACACG 
TTAGCTGATT TTATCAGAAA TTCAGTCCAG TCACCGTTTG GTCTGGAACA GCTTAATGGC 
TGGGTCACTA GCAAGGAGAA AGAGATGAAT GTCGTCAAAG CTTACATGAA ACTCTTTTCG 
GGTATTCAAA TTATCAGGTC TCAGGCAGAC TTAGACGAGC TTGTATTAGA CCCGTTGGTG 
AGCAACGCTG TCTGCTTTGT CTTCCCTCTC GATGGACCTG AACAATACTT TGAGGCCTTG 
TCCCAGCATT TGCAATCTGC CGATGGACCT GCATCTAATT TGAGCATACA TTCGGACTGG 
TTTGCTGATA AGTCCGCATC TGCAAATATG AGATCTGATG CCAGAGGTTT TGCAGAGTTT 
TGCCGGGCCA ATGCTGGTGC CGAAACCAAC GAAACTGTTT TTGCAGTCAC AGGATTTGGC 
GCAGACGTAG GAGGGACAGG TTCGATTTTG CTGTACGAAG AGGGCGTACT AACAACAAAG 
GGTTTTAAAC CACCAAGTAA ACCCGGAAAG CCGTCGATAC AGAGCAAGAC CAATGATGAA 
GTAACTCTCG AGTGGTTACC TCCGGAAATG GGCCTGTCGG AGGTGGAGCG CTATGAGGTG 
CAATACCAAG AAACAGGAAA GGGTCTACGA TCGACAAGCC CATGGGATTG GCAGAAATCA 
TGTACTAGAT CGGACACGAG ACAAATCACC ATCCAAAACT TAAAGCCTCT CACAACCTAT 
GAGTTTCAAG TTGTCGCTGT TTGTCGAGTT GGTGTCAGTC AGGTGAGTGA AAGTAGTGAT 
CCATGTACAA CACTTCCTGC TGGTCCTCCG ATACGTGTTC GCAAGCATGC AGTGTCTGAA 
ACATGTCTGA CCATCGTTTG GGATCCGCCG GCTGTATTGG GTGAAGGTGT GCAGGTTGAG 
AATTACGTGG TCAGATACAC ACAGGTTTCA GCGCTTTCCA TTGCTGGTCC AAATCAAGAT 
GTTGTGAAAT CCACGAGCGC TGATACATGC CTGTTCATTA TTGATGACCT CAAACCCAAC 
GAACCATACA GAATTTGGGT GTCCGCGCAG TGTGGCAGTG CTGGAAATGG CGTGGAAAGT 
GAGCCACTAG AAATCGCCAC GGATCAAGCT CACCAGCCTG CAAAGTATGC GAAACGAATA 
GCTAAGACTG CCGAAGTACT CTCAGAAGGA TGCGAGTCAA CTGGTACACC AACTATTCTG 
AAGATTCCCT TAGACCAACA TTCCACCGGC TCGGGTGGAA ACTACCTAAG CTTCAGCTTC 
GGCAAGAAAA CTAGCATGGT TCAGCAGCAC AAAGTGATAA TGGTTGTTGG AGCCACGGGT 
GCTGGTAAGA GCACTCTAAT CAATGGGATG ATCAACTACA TCCTTAAAGT AGACTGGAAA 
GATGGGTTTC GGTTCAAGAT AGTTGACCAG CGTGGAGAGG GACCAGAGTC ACAAGCGCAC 
AGTCAAACCC AAATCATATC GGCATACACC GTTTACGAAA ACAAACACCT CAACGTCCCA 
TACACATTGA CAATCATTGA CACGCCAGGA TTTGGAGACA CTAGAGGCAT TGAGCGTGAC 
CGGGCCATCG TTGATCAGAT ACGAGATTTT TTCTCTCTTT CAGAGGCACA CAAAGTGGAT 
CACATAGACG CGATTGGCTT CGTTGCTCAG TCTTCGCAAG CACGCCTGAC CCACACTCAA 
CGATATGTTT TTGATTCCAT CCTCTCCGTC TTTGGTAAAG ACATCTCGTC TAACATACTT 
CTTCTTGTCA CTTTCTGTGA CGGGCAAAAG CCCCCGGTTG TCGATGCGGT TAAAGAAGCA 
GACATTCCAT GCAGTAACAG CATGTTCAAA TTCAACAACT CAGCTCTTTT TGCGAGCAAT 
GGAATTTCCA AAGGGTTAGA AACAGGGGGA AAGGCTTTCA CAGATACCAA TGACATTACC 
AAGAAGATAA TTTCGACAGG ATGTTCTGGC GGATGGGAAT GCAAAGCATG TGGAACTTCT 
TCAACGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022109329.1
CDS	777..3788
Translation	MAEQGVLELATLGRPMQLGMLYDCRRDSLIPGLTLWDPAVLQNDRDVRPQPSTQYDIMSSDRVDDKASSLNISASLRASFLGGLIEVKGSAKFLHDKKSSIKQARLTLHYKTTTRFEQLTMKHLGAHNVQHSDIFDQGTATHVVTGILYGAHAFFVFDRDCTREEEIKDVQGRMHGMVKRIASFEAGGEGSLEEGGREGVDKMRCSFHGDFNLENNPASYQDAVSVYTKLPLMLGRDGEYAVPIRAWLYPLDKLDQKAAKLTRQISVNLISQSQEILEGLEDVGVKCDDMIMSDICRDFPQFREKFGPFKQKIVQYKLYLQEYLVQALPAIRGGSVEENTLADFIRNSVQSPFGLEQLNGWVTSKEKEMNVVKAYMKLFSGIQIIRSQADLDELVLDPLVSNAVCFVFPLDGPEQYFEALSQHLQSADGPASNLSIHSDWFADKSASANMRSDARGFAEFCRANAGAETNETVFAVTGFGADVGGTGSILLYEEGVLTTKGFKPPSKPGKPSIQSKTNDEVTLEWLPPEMGLSEVERYEVQYQETGKGLRSTSPWDWQKSCTRSDTRQITIQNLKPLTTYEFQVVAVCRVGVSQVSESSDPCTTLPAGPPIRVRKHAVSETCLTIVWDPPAVLGEGVQVENYVVRYTQVSALSIAGPNQDVVKSTSADTCLFIIDDLKPNEPYRIWVSAQCGSAGNGVESEPLEIATDQAHQPAKYAKRIAKTAEVLSEGCESTGTPTILKIPLDQHSTGSGGNYLSFSFGKKTSMVQQHKVIMVVGATGAGKSTLINGMINYILKVDWKDGFRFKIVDQRGEGPESQAHSQTQIISAYTVYENKHLNVPYTLTIIDTPGFGDTRGIERDRAIVDQIRDFFSLSEAHKVDHIDAIGFVAQSSQARLTHTQRYVFDSILSVFGKDISSNILLLVTFCDGQKPPVVDAVKEADIPCSNSMFKFNNSALFASNGISKGLETGGKAFTDTNDITKKIISTGCSGGWECKACGTSSTP

Target ORF information:

RefSeq Version	XM_022253637.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253637.1

ORF Insert Sequence:

ATGGCAGAAC AAGGCGTGTT AGAGCTGGCT ACCTTAGGGC GTCCCATGCA GCTGGGTATG 
CTTTACGATT GCCGTAGAGA CAGTCTGATT CCTGGATTAA CTCTCTGGGA TCCAGCTGTT 
TTGCAGAATG ATCGTGACGT GCGTCCTCAG CCCAGCACAC AGTACGACAT CATGTCATCT 
GACAGAGTGG ACGACAAGGC ATCCTCTCTC AACATCAGCG CGTCGTTGAG GGCTAGCTTC 
TTGGGTGGAC TCATAGAGGT CAAAGGCTCG GCCAAGTTCC TTCACGACAA AAAGTCTTCT 
ATTAAACAAG CTCGCCTTAC ACTGCATTAC AAAACTACCA CAAGGTTTGA GCAGTTGACA 
ATGAAGCATC TAGGTGCACA CAATGTTCAG CATTCTGACA TATTTGACCA AGGTACAGCT 
ACTCATGTTG TAACAGGTAT CCTCTATGGG GCCCATGCAT TTTTCGTATT CGATAGGGAC 
TGTACGAGAG AGGAAGAAAT CAAAGATGTG CAGGGGCGAA TGCATGGCAT GGTGAAAAGA 
ATAGCTTCAT TTGAAGCGGG TGGGGAGGGG TCTCTAGAGG AGGGTGGACG AGAGGGTGTT 
GATAAGATGC GCTGTAGTTT CCACGGTGAC TTTAACCTAG AAAATAACCC GGCAAGTTAC 
CAAGACGCTG TCAGCGTCTA TACGAAACTG CCCTTAATGC TTGGCAGGGA CGGGGAATAT 
GCGGTTCCCA TACGGGCTTG GCTATATCCT TTAGATAAGT TGGATCAAAA AGCTGCCAAG 
TTGACTCGTC AAATAAGTGT CAACTTAATA AGTCAATCCC AGGAGATTTT GGAAGGCTTG 
GAGGACGTGG GGGTTAAATG CGACGACATG ATTATGTCGG ACATTTGCAG AGATTTCCCC 
CAGTTTCGGG AGAAGTTCGG TCCGTTTAAA CAGAAGATCG TCCAGTATAA ATTGTATTTA 
CAAGAGTATC TGGTGCAAGC ATTGCCGGCA ATCCGAGGGG GCAGTGTTGA GGAGAACACG 
TTAGCTGATT TTATCAGAAA TTCAGTCCAG TCACCGTTTG GTCTGGAACA GCTTAATGGC 
TGGGTCACTA GCAAGGAGAA AGAGATGAAT GTCGTCAAAG CTTACATGAA ACTCTTTTCG 
GGTATTCAAA TTATCAGGTC TCAGGCAGAC TTAGACGAGC TTGTATTAGA CCCGTTGGTG 
AGCAACGCTG TCTGCTTTGT CTTCCCTCTC GATGGACCTG AACAATACTT TGAGGCCTTG 
TCCCAGCATT TGCAATCTGC CGATGGACCT GCATCTAATT TGAGCATACA TTCGGACTGG 
TTTGCTGATA AGTCCGCATC TGCAAATATG AGATCTGATG CCAGAGGTTT TGCAGAGTTT 
TGCCGGGCCA ATGCTGGTGC CGAAACCAAC GAAACTGTTT TTGCAGTCAC AGGATTTGGC 
GCAGACGTAG GAGGGACAGG TTCGATTTTG CTGTACGAAG AGGGCGTACT AACAACAAAG 
GGTTTTAAAC CACCAAGTAA ACCCGGAAAG CCGTCGATAC AGAGCAAGAC CAATGATGAA 
GTAACTCTCG AGTGGTTACC TCCGGAAATG GGCCTGTCGG AGGTGGAGCG CTATGAGGTG 
CAATACCAAG AAACAGGAAA GGGTCTACGA TCGACAAGCC CATGGGATTG GCAGAAATCA 
TGTACTAGAT CGGACACGAG ACAAATCACC ATCCAAAACT TAAAGCCTCT CACAACCTAT 
GAGTTTCAAG TTGTCGCTGT TTGTCGAGTT GGTGTCAGTC AGGTGAGTGA AAGTAGTGAT 
CCATGTACAA CACTTCCTGC TGGTCCTCCG ATACGTGTTC GCAAGCATGC AGTGTCTGAA 
ACATGTCTGA CCATCGTTTG GGATCCGCCG GCTGTATTGG GTGAAGGTGT GCAGGTTGAG 
AATTACGTGG TCAGATACAC ACAGGTTTCA GCGCTTTCCA TTGCTGGTCC AAATCAAGAT 
GTTGTGAAAT CCACGAGCGC TGATACATGC CTGTTCATTA TTGATGACCT CAAACCCAAC 
GAACCATACA GAATTTGGGT GTCCGCGCAG TGTGGCAGTG CTGGAAATGG CGTGGAAAGT 
GAGCCACTAG AAATCGCCAC GGATCAAGCT CACCAGCCTG CAAAGTATGC GAAACGAATA 
GCTAAGACTG CCGAAGTACT CTCAGAAGGA TGCGAGTCAA CTGGTACACC AACTATTCTG 
AAGATTCCCT TAGACCAACA TTCCACCGGC TCGGGTGGAA ACTACCTAAG CTTCAGCTTC 
GGCAAGAAAA CTAGCATGGT TCAGCAGCAC AAAGTGATAA TGGTTGTTGG AGCCACGGGT 
GCTGGTAAGA GCACTCTAAT CAATGGGATG ATCAACTACA TCCTTAAAGT AGACTGGAAA 
GATGGGTTTC GGTTCAAGAT AGTTGACCAG CGTGGAGAGG GACCAGAGTC ACAAGCGCAC 
AGTCAAACCC AAATCATATC GGCATACACC GTTTACGAAA ACAAACACCT CAACGTCCCA 
TACACATTGA CAATCATTGA CACGCCAGGA TTTGGAGACA CTAGAGGCAT TGAGCGTGAC 
CGGGCCATCG TTGATCAGAT ACGAGATTTT TTCTCTCTTT CAGAGGCACA CAAAGTGGAT 
CACATAGACG CGATTGGCTT CGTTGCTCAG TCTTCGCAAG CACGCCTGAC CCACACTCAA 
CGATATGTTT TTGATTCCAT CCTCTCCGTC TTTGGTAAAG ACATCTCGTC TAACATACTT 
CTTCTTGTCA CTTTCTGTGA CGGGCAAAAG CCCCCGGTTG TCGATGCGGT TAAAGAAGCA 
GACATTCCAT GCAGTAACAG CATGTTCAAA TTCAACAACT CAGCTCTTTT TGCGAGCAAT 
GGAATTTCCA AAGGGTTAGA AACAGGGGGA AAGGCTTTCA CAGATACCAA TGACATTACC 
AAGAAGATAA TTTCGACAGG ATGTTCTGGC GGATGGGAAT GCAAAGCATG TGGAACTTCT 
TCAACGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110989330 ( XM_022253638.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022109330.1 Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc172521
Clone ID Related Accession (Same CDS sequence)	XM_022253637.1 , XM_022253638.1
Accession Version	XM_022253638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	stonustoxin subunit beta-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091638.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC AAGGCGTGTT AGAGCTGGCT ACCTTAGGGC GTCCCATGCA GCTGGGTATG 
CTTTACGATT GCCGTAGAGA CAGTCTGATT CCTGGATTAA CTCTCTGGGA TCCAGCTGTT 
TTGCAGAATG ATCGTGACGT GCGTCCTCAG CCCAGCACAC AGTACGACAT CATGTCATCT 
GACAGAGTGG ACGACAAGGC ATCCTCTCTC AACATCAGCG CGTCGTTGAG GGCTAGCTTC 
TTGGGTGGAC TCATAGAGGT CAAAGGCTCG GCCAAGTTCC TTCACGACAA AAAGTCTTCT 
ATTAAACAAG CTCGCCTTAC ACTGCATTAC AAAACTACCA CAAGGTTTGA GCAGTTGACA 
ATGAAGCATC TAGGTGCACA CAATGTTCAG CATTCTGACA TATTTGACCA AGGTACAGCT 
ACTCATGTTG TAACAGGTAT CCTCTATGGG GCCCATGCAT TTTTCGTATT CGATAGGGAC 
TGTACGAGAG AGGAAGAAAT CAAAGATGTG CAGGGGCGAA TGCATGGCAT GGTGAAAAGA 
ATAGCTTCAT TTGAAGCGGG TGGGGAGGGG TCTCTAGAGG AGGGTGGACG AGAGGGTGTT 
GATAAGATGC GCTGTAGTTT CCACGGTGAC TTTAACCTAG AAAATAACCC GGCAAGTTAC 
CAAGACGCTG TCAGCGTCTA TACGAAACTG CCCTTAATGC TTGGCAGGGA CGGGGAATAT 
GCGGTTCCCA TACGGGCTTG GCTATATCCT TTAGATAAGT TGGATCAAAA AGCTGCCAAG 
TTGACTCGTC AAATAAGTGT CAACTTAATA AGTCAATCCC AGGAGATTTT GGAAGGCTTG 
GAGGACGTGG GGGTTAAATG CGACGACATG ATTATGTCGG ACATTTGCAG AGATTTCCCC 
CAGTTTCGGG AGAAGTTCGG TCCGTTTAAA CAGAAGATCG TCCAGTATAA ATTGTATTTA 
CAAGAGTATC TGGTGCAAGC ATTGCCGGCA ATCCGAGGGG GCAGTGTTGA GGAGAACACG 
TTAGCTGATT TTATCAGAAA TTCAGTCCAG TCACCGTTTG GTCTGGAACA GCTTAATGGC 
TGGGTCACTA GCAAGGAGAA AGAGATGAAT GTCGTCAAAG CTTACATGAA ACTCTTTTCG 
GGTATTCAAA TTATCAGGTC TCAGGCAGAC TTAGACGAGC TTGTATTAGA CCCGTTGGTG 
AGCAACGCTG TCTGCTTTGT CTTCCCTCTC GATGGACCTG AACAATACTT TGAGGCCTTG 
TCCCAGCATT TGCAATCTGC CGATGGACCT GCATCTAATT TGAGCATACA TTCGGACTGG 
TTTGCTGATA AGTCCGCATC TGCAAATATG AGATCTGATG CCAGAGGTTT TGCAGAGTTT 
TGCCGGGCCA ATGCTGGTGC CGAAACCAAC GAAACTGTTT TTGCAGTCAC AGGATTTGGC 
GCAGACGTAG GAGGGACAGG TTCGATTTTG CTGTACGAAG AGGGCGTACT AACAACAAAG 
GGTTTTAAAC CACCAAGTAA ACCCGGAAAG CCGTCGATAC AGAGCAAGAC CAATGATGAA 
GTAACTCTCG AGTGGTTACC TCCGGAAATG GGCCTGTCGG AGGTGGAGCG CTATGAGGTG 
CAATACCAAG AAACAGGAAA GGGTCTACGA TCGACAAGCC CATGGGATTG GCAGAAATCA 
TGTACTAGAT CGGACACGAG ACAAATCACC ATCCAAAACT TAAAGCCTCT CACAACCTAT 
GAGTTTCAAG TTGTCGCTGT TTGTCGAGTT GGTGTCAGTC AGGTGAGTGA AAGTAGTGAT 
CCATGTACAA CACTTCCTGC TGGTCCTCCG ATACGTGTTC GCAAGCATGC AGTGTCTGAA 
ACATGTCTGA CCATCGTTTG GGATCCGCCG GCTGTATTGG GTGAAGGTGT GCAGGTTGAG 
AATTACGTGG TCAGATACAC ACAGGTTTCA GCGCTTTCCA TTGCTGGTCC AAATCAAGAT 
GTTGTGAAAT CCACGAGCGC TGATACATGC CTGTTCATTA TTGATGACCT CAAACCCAAC 
GAACCATACA GAATTTGGGT GTCCGCGCAG TGTGGCAGTG CTGGAAATGG CGTGGAAAGT 
GAGCCACTAG AAATCGCCAC GGATCAAGCT CACCAGCCTG CAAAGTATGC GAAACGAATA 
GCTAAGACTG CCGAAGTACT CTCAGAAGGA TGCGAGTCAA CTGGTACACC AACTATTCTG 
AAGATTCCCT TAGACCAACA TTCCACCGGC TCGGGTGGAA ACTACCTAAG CTTCAGCTTC 
GGCAAGAAAA CTAGCATGGT TCAGCAGCAC AAAGTGATAA TGGTTGTTGG AGCCACGGGT 
GCTGGTAAGA GCACTCTAAT CAATGGGATG ATCAACTACA TCCTTAAAGT AGACTGGAAA 
GATGGGTTTC GGTTCAAGAT AGTTGACCAG CGTGGAGAGG GACCAGAGTC ACAAGCGCAC 
AGTCAAACCC AAATCATATC GGCATACACC GTTTACGAAA ACAAACACCT CAACGTCCCA 
TACACATTGA CAATCATTGA CACGCCAGGA TTTGGAGACA CTAGAGGCAT TGAGCGTGAC 
CGGGCCATCG TTGATCAGAT ACGAGATTTT TTCTCTCTTT CAGAGGCACA CAAAGTGGAT 
CACATAGACG CGATTGGCTT CGTTGCTCAG TCTTCGCAAG CACGCCTGAC CCACACTCAA 
CGATATGTTT TTGATTCCAT CCTCTCCGTC TTTGGTAAAG ACATCTCGTC TAACATACTT 
CTTCTTGTCA CTTTCTGTGA CGGGCAAAAG CCCCCGGTTG TCGATGCGGT TAAAGAAGCA 
GACATTCCAT GCAGTAACAG CATGTTCAAA TTCAACAACT CAGCTCTTTT TGCGAGCAAT 
GGAATTTCCA AAGGGTTAGA AACAGGGGGA AAGGCTTTCA CAGATACCAA TGACATTACC 
AAGAAGATAA TTTCGACAGG ATGTTCTGGC GGATGGGAAT GCAAAGCATG TGGAACTTCT 
TCAACGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022109330.1
CDS	164..3175
Translation	MAEQGVLELATLGRPMQLGMLYDCRRDSLIPGLTLWDPAVLQNDRDVRPQPSTQYDIMSSDRVDDKASSLNISASLRASFLGGLIEVKGSAKFLHDKKSSIKQARLTLHYKTTTRFEQLTMKHLGAHNVQHSDIFDQGTATHVVTGILYGAHAFFVFDRDCTREEEIKDVQGRMHGMVKRIASFEAGGEGSLEEGGREGVDKMRCSFHGDFNLENNPASYQDAVSVYTKLPLMLGRDGEYAVPIRAWLYPLDKLDQKAAKLTRQISVNLISQSQEILEGLEDVGVKCDDMIMSDICRDFPQFREKFGPFKQKIVQYKLYLQEYLVQALPAIRGGSVEENTLADFIRNSVQSPFGLEQLNGWVTSKEKEMNVVKAYMKLFSGIQIIRSQADLDELVLDPLVSNAVCFVFPLDGPEQYFEALSQHLQSADGPASNLSIHSDWFADKSASANMRSDARGFAEFCRANAGAETNETVFAVTGFGADVGGTGSILLYEEGVLTTKGFKPPSKPGKPSIQSKTNDEVTLEWLPPEMGLSEVERYEVQYQETGKGLRSTSPWDWQKSCTRSDTRQITIQNLKPLTTYEFQVVAVCRVGVSQVSESSDPCTTLPAGPPIRVRKHAVSETCLTIVWDPPAVLGEGVQVENYVVRYTQVSALSIAGPNQDVVKSTSADTCLFIIDDLKPNEPYRIWVSAQCGSAGNGVESEPLEIATDQAHQPAKYAKRIAKTAEVLSEGCESTGTPTILKIPLDQHSTGSGGNYLSFSFGKKTSMVQQHKVIMVVGATGAGKSTLINGMINYILKVDWKDGFRFKIVDQRGEGPESQAHSQTQIISAYTVYENKHLNVPYTLTIIDTPGFGDTRGIERDRAIVDQIRDFFSLSEAHKVDHIDAIGFVAQSSQARLTHTQRYVFDSILSVFGKDISSNILLLVTFCDGQKPPVVDAVKEADIPCSNSMFKFNNSALFASNGISKGLETGGKAFTDTNDITKKIISTGCSGGWECKACGTSSTP

Target ORF information:

RefSeq Version	XM_022253638.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci stonustoxin subunit beta-like (LOC110989330), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253638.1

ORF Insert Sequence:

ATGGCAGAAC AAGGCGTGTT AGAGCTGGCT ACCTTAGGGC GTCCCATGCA GCTGGGTATG 
CTTTACGATT GCCGTAGAGA CAGTCTGATT CCTGGATTAA CTCTCTGGGA TCCAGCTGTT 
TTGCAGAATG ATCGTGACGT GCGTCCTCAG CCCAGCACAC AGTACGACAT CATGTCATCT 
GACAGAGTGG ACGACAAGGC ATCCTCTCTC AACATCAGCG CGTCGTTGAG GGCTAGCTTC 
TTGGGTGGAC TCATAGAGGT CAAAGGCTCG GCCAAGTTCC TTCACGACAA AAAGTCTTCT 
ATTAAACAAG CTCGCCTTAC ACTGCATTAC AAAACTACCA CAAGGTTTGA GCAGTTGACA 
ATGAAGCATC TAGGTGCACA CAATGTTCAG CATTCTGACA TATTTGACCA AGGTACAGCT 
ACTCATGTTG TAACAGGTAT CCTCTATGGG GCCCATGCAT TTTTCGTATT CGATAGGGAC 
TGTACGAGAG AGGAAGAAAT CAAAGATGTG CAGGGGCGAA TGCATGGCAT GGTGAAAAGA 
ATAGCTTCAT TTGAAGCGGG TGGGGAGGGG TCTCTAGAGG AGGGTGGACG AGAGGGTGTT 
GATAAGATGC GCTGTAGTTT CCACGGTGAC TTTAACCTAG AAAATAACCC GGCAAGTTAC 
CAAGACGCTG TCAGCGTCTA TACGAAACTG CCCTTAATGC TTGGCAGGGA CGGGGAATAT 
GCGGTTCCCA TACGGGCTTG GCTATATCCT TTAGATAAGT TGGATCAAAA AGCTGCCAAG 
TTGACTCGTC AAATAAGTGT CAACTTAATA AGTCAATCCC AGGAGATTTT GGAAGGCTTG 
GAGGACGTGG GGGTTAAATG CGACGACATG ATTATGTCGG ACATTTGCAG AGATTTCCCC 
CAGTTTCGGG AGAAGTTCGG TCCGTTTAAA CAGAAGATCG TCCAGTATAA ATTGTATTTA 
CAAGAGTATC TGGTGCAAGC ATTGCCGGCA ATCCGAGGGG GCAGTGTTGA GGAGAACACG 
TTAGCTGATT TTATCAGAAA TTCAGTCCAG TCACCGTTTG GTCTGGAACA GCTTAATGGC 
TGGGTCACTA GCAAGGAGAA AGAGATGAAT GTCGTCAAAG CTTACATGAA ACTCTTTTCG 
GGTATTCAAA TTATCAGGTC TCAGGCAGAC TTAGACGAGC TTGTATTAGA CCCGTTGGTG 
AGCAACGCTG TCTGCTTTGT CTTCCCTCTC GATGGACCTG AACAATACTT TGAGGCCTTG 
TCCCAGCATT TGCAATCTGC CGATGGACCT GCATCTAATT TGAGCATACA TTCGGACTGG 
TTTGCTGATA AGTCCGCATC TGCAAATATG AGATCTGATG CCAGAGGTTT TGCAGAGTTT 
TGCCGGGCCA ATGCTGGTGC CGAAACCAAC GAAACTGTTT TTGCAGTCAC AGGATTTGGC 
GCAGACGTAG GAGGGACAGG TTCGATTTTG CTGTACGAAG AGGGCGTACT AACAACAAAG 
GGTTTTAAAC CACCAAGTAA ACCCGGAAAG CCGTCGATAC AGAGCAAGAC CAATGATGAA 
GTAACTCTCG AGTGGTTACC TCCGGAAATG GGCCTGTCGG AGGTGGAGCG CTATGAGGTG 
CAATACCAAG AAACAGGAAA GGGTCTACGA TCGACAAGCC CATGGGATTG GCAGAAATCA 
TGTACTAGAT CGGACACGAG ACAAATCACC ATCCAAAACT TAAAGCCTCT CACAACCTAT 
GAGTTTCAAG TTGTCGCTGT TTGTCGAGTT GGTGTCAGTC AGGTGAGTGA AAGTAGTGAT 
CCATGTACAA CACTTCCTGC TGGTCCTCCG ATACGTGTTC GCAAGCATGC AGTGTCTGAA 
ACATGTCTGA CCATCGTTTG GGATCCGCCG GCTGTATTGG GTGAAGGTGT GCAGGTTGAG 
AATTACGTGG TCAGATACAC ACAGGTTTCA GCGCTTTCCA TTGCTGGTCC AAATCAAGAT 
GTTGTGAAAT CCACGAGCGC TGATACATGC CTGTTCATTA TTGATGACCT CAAACCCAAC 
GAACCATACA GAATTTGGGT GTCCGCGCAG TGTGGCAGTG CTGGAAATGG CGTGGAAAGT 
GAGCCACTAG AAATCGCCAC GGATCAAGCT CACCAGCCTG CAAAGTATGC GAAACGAATA 
GCTAAGACTG CCGAAGTACT CTCAGAAGGA TGCGAGTCAA CTGGTACACC AACTATTCTG 
AAGATTCCCT TAGACCAACA TTCCACCGGC TCGGGTGGAA ACTACCTAAG CTTCAGCTTC 
GGCAAGAAAA CTAGCATGGT TCAGCAGCAC AAAGTGATAA TGGTTGTTGG AGCCACGGGT 
GCTGGTAAGA GCACTCTAAT CAATGGGATG ATCAACTACA TCCTTAAAGT AGACTGGAAA 
GATGGGTTTC GGTTCAAGAT AGTTGACCAG CGTGGAGAGG GACCAGAGTC ACAAGCGCAC 
AGTCAAACCC AAATCATATC GGCATACACC GTTTACGAAA ACAAACACCT CAACGTCCCA 
TACACATTGA CAATCATTGA CACGCCAGGA TTTGGAGACA CTAGAGGCAT TGAGCGTGAC 
CGGGCCATCG TTGATCAGAT ACGAGATTTT TTCTCTCTTT CAGAGGCACA CAAAGTGGAT 
CACATAGACG CGATTGGCTT CGTTGCTCAG TCTTCGCAAG CACGCCTGAC CCACACTCAA 
CGATATGTTT TTGATTCCAT CCTCTCCGTC TTTGGTAAAG ACATCTCGTC TAACATACTT 
CTTCTTGTCA CTTTCTGTGA CGGGCAAAAG CCCCCGGTTG TCGATGCGGT TAAAGAAGCA 
GACATTCCAT GCAGTAACAG CATGTTCAAA TTCAACAACT CAGCTCTTTT TGCGAGCAAT 
GGAATTTCCA AAGGGTTAGA AACAGGGGGA AAGGCTTTCA CAGATACCAA TGACATTACC 
AAGAAGATAA TTTCGACAGG ATGTTCTGGC GGATGGGAAT GCAAAGCATG TGGAACTTCT 
TCAACGCCCT GA