ACA1_046640 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_046640
Entrez Gene ID	14922862
Full Name	ankyrin repeat-containing protein
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004347726.1	XP_004347776.1	ankyrin repeat-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_046640 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_046640 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc111341	XM_004347726.1 Latest version!	Acanthamoeba castellanii str. Neff ankyrin repeat-containing protein (ACA1_046640) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_046640 ( XM_004347726.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004347776.1 Acanthamoeba castellanii str. Neff ankyrin repeat-containing protein (ACA1_046640) mRNA, complete cds.

Price & Availability↑

CloneID	OAc111341
Clone ID Related Accession (Same CDS sequence)	XM_004347726.1
Accession Version	XM_004347726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	ankyrin repeat-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457606). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGGTCGAAT CCTTTCCCAA TGTGGAGACC CTGGCCTTGG CCTGCGCCGA ACTCGAGAGC 
TTAAACGGGA AGGGTTACTT CTCGCTGACT CAAGAGCTGA GCTATCTAAC GAACCTCAAG 
CACTTGGACA TTTCCTACGT CCCGGTGGAC AAGAGCGACG AGAGCCTAGC GACCAATTTG 
AACTTGAAGA CTCTCATCTC GATTCAAGAA ACCGGCCCTT ACGGGAACAG ACCAACGCGC 
GTCAGACCTC ATCACAGGGA CGCCATGACC GCCAAATACA TCTTCGCTCA GGGTGGCCTC 
GATGATCAGA GCCTGGTCTC GTCTGTGACC AATCCGGCTT GCTTTGACCT CGCCGGCCGC 
ACGCTTTTGC ATCGCGCCGC GGATGACCCC AAGCGCATTG CACAGCTGCG ACAGTTGGTG 
GACGAATCGG GCCACGTCGA CGCGCCGCTT GCTGCCGGAT TGGACAATGC TGTTGGCGTC 
ACGGACATGT ACAATCGGTT CATCCACAGC GCCAAGCGCA GGGTAAGCGA TCGGTTGGGC 
GGGTTGAGCT TCCCGTCGCC CGTCTCGTCC GAGAGACGCA ATCAGGCGAC AGCCATGCAA 
ACCAGACCCA TGCTGCTCAC ACCTCAGGCC GAAGCCCCCG GGTCGACCGC ACTTCACATA 
GCGTGCACGA ATGACAACCA CGAGGCGGTG GCGTACCTGC TGGCCAAGGG CGCTGACGTG 
AACGCTATGA ATGACATAGG GCAAACCCCG TTGATGTTGG CGTGTCACTA CGGCAACCTG 
CTGGCGGTTT CGGCATTGGT TGCCACCGGG CGCGTCCTCA TCAACCTCCA GGACAATCAG 
CTCTTCGAAC TTCTCTTTGG TGCGGGCGCC AACGTCAACC AGCCCAACTA CAATGGATTC 
TCGGCGCTTC ACTTGGCCTG CATGGCCCGC AACCCTGCTC TCGTGGTCTA CCTGCTCTCG 
CGCGGCGCCT CGGTGAGTGT GGGGATCAGC CCGCTTTTCT GCCTGGCTGG CGATGGCGAG 
CTCTCGGTTT TCGCCACCCA GTCACGGGAT GTCGGCGTCT TGGTCGACGT TCTTGTCGAC 
GCTGGCTGCC CGGTCAACGC GGTCAACGAG ACGGGCAAAA CGTTGCTGCA CGTGGCCTGC 
CAGGACCGCG ACGCAGACTT GGCCGAGGCC CTGCTGCGCC ATGCCGACGT CGACGTAAAC 
TTGGCCGATG CTGTCGGATG GGCAGAGCGT TCGCTCCGTC TTCGATGCCG TCCCGCCGCG 
CCGCCCTCGC CGCCGCGACC GGGCCTCGCA TCCATGCTTC TCAGTCGACA GGCCAACGTC 
AATGCCACCG CCACTGACCC CCGACCAGAA GAGGACAAGA AGGAGAAAAG CAAGGTTGTC 
TCCTTCATGC GCAAGCTCAT CAAATCAGGC GCGGACGTCA ACGCCAAGGA CGCTCGAGGG 
TGGACCGCCC TGCACGCAGT GTGCGCTTCG AGCCACCTCG AAGCCGTTGG TTTGCTGTTG 
GCCCATGGCG CCAACGTCAA TGCCCGCGAT TTCAAGTACT CGCACGCTCA GCTCCTCGGC 
CCTAACAACT CGGAGTTTGA AGTGCGAGCA CCGGCGATAG TGGGACGGTC CGGACCAATC 
CAGAGGCGGG AACAACCACC TGCGAGTGAG GCCGACTCGT GGACCATTTG CAAGTTGCTC 
CTCGGCCACG GTGCTCAGTT CGATGTTGTC GATGAAGTCG AGGGCGAGAC TGCGCTGCAC 
TTGTTGACGC GCGCCGGCAC CGGACCACAA AACGGTCTGC TGGCCTGGGC TGTCGTGCAG 
CTCAAGGACC ACGGGGTCTT CCACCTGCGA AATGCCAAGG GCGAGACTCT GCTGCACAGT 
GCGATTGCTT GCAACAACTC CAAGCTGGTG TCGATTCTCT TGCAGCACGG CGCCGACAAG 
AGCCTCAGGT CCCACCTGGG CGAAACTGCG CTCCACACGG CACTGCGCCT GCGCCTTCAG 
AGTGCGACGC GTATTCGAAG TCTGTCCGAA CAGCCCGCCG AGTCCTGCGA TTCGGCCCTC 
TGGCCCACCT CGGCCCAGAT CTCGCCGCAC CAAGCCTATT GGCCCACCAG CGAGGTCAGC 
GAGCAGAACA TCGTGGCCCT CGTGGCTCGC GGGCTGACCG AAGAAGAGTG CACTGCGAGG 
GACTGCTTCG GCCTCACCGC GCTCCACTGG GCCGTCGCTT TGGGTGCCCT GCCCCTGCCC 
CACTTCCTCT TCCTGTCCCA CGCCGCCGCG TGGCTCGGAG CGAATGCCCC GAGGGCAACG 
CAGGACAAGA GCCTGTCGCG CCTTGCGGTC CTGTCGCTCT TTATCGTGAA CCACATCCTG 
ACTCACTTCA AGGACCGAGT GATGGATGCA GTGGATGAGC GTGGCGACAC GGCGCTACAC 
AGGCTGGCCG AAATGCGGCG GATCACTACT CTCATGGCTG TTTCTTCTCA GGCCAGTTTG 
ATCGTAAGAG CTCAGATGCC GACATCGACG ACGTCTTCCT GCGGGAGGCC TTCGGGGAGC 
TCACTTCGGC GGCGTCGCAG ATCGAGCAAC GCCTCTTTTC GCGGGAGGGC ACGACGACGA 
TCGAGGAGGA GCCGCTCCAC AAGCGGCGGA CCTCCCCCGC AGCAGCAGCA GCTGTTGTGG 
AGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004347776.1
CDS	1..2649
Translation	MVESFPNVETLALACAELESLNGKGYFSLTQELSYLTNLKHLDISYVPVDKSDESLATNLNLKTLISIQETGPYGNRPTRVRPHHRDAMTAKYIFAQGGLDDQSLVSSVTNPACFDLAGRTLLHRAADDPKRIAQLRQLVDESGHVDAPLAAGLDNAVGVTDMYNRFIHSAKRRVSDRLGGLSFPSPVSSERRNQATAMQTRPMLLTPQAEAPGSTALHIACTNDNHEAVAYLLAKGADVNAMNDIGQTPLMLACHYGNLLAVSALVATGRVLINLQDNQLFELLFGAGANVNQPNYNGFSALHLACMARNPALVVYLLSRGASVSVGISPLFCLAGDGELSVFATQSRDVGVLVDVLVDAGCPVNAVNETGKTLLHVACQDRDADLAEALLRHADVDVNLADAVGWAERSLRLRCRPAAPPSPPRPGLASMLLSRQANVNATATDPRPEEDKKEKSKVVSFMRKLIKSGADVNAKDARGWTALHAVCASSHLEAVGLLLAHGANVNARDFKYSHAQLLGPNNSEFEVRAPAIVGRSGPIQRREQPPASEADSWTICKLLLGHGAQFDVVDEVEGETALHLLTRAGTGPQNGLLAWAVVQLKDHGVFHLRNAKGETLLHSAIACNNSKLVSILLQHGADKSLRSHLGETALHTALRLRLQSATRIRSLSEQPAESCDSALWPTSAQISPHQAYWPTSEVSEQNIVALVARGLTEEECTARDCFGLTALHWAVALGALPLPHFLFLSHAAAWLGANAPRATQDKSLSRLAVLSLFIVNHILTHFKDRVMDAVDERGDTALHRLAEMRRITTLMAVSSQASLIVRAQMPTSTTSSCGRPSGSSLRRRRRSSNASFRGRARRRSRRSRSTSGGPPPQQQQLLWSS

Target ORF information:

RefSeq Version	XM_004347726.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff ankyrin repeat-containing protein (ACA1_046640) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004347726.1

ORF Insert Sequence:

ATGGTCGAAT CCTTTCCCAA TGTGGAGACC CTGGCCTTGG CCTGCGCCGA ACTCGAGAGC 
TTAAACGGGA AGGGTTACTT CTCGCTGACT CAAGAGCTGA GCTATCTAAC GAACCTCAAG 
CACTTGGACA TTTCCTACGT CCCGGTGGAC AAGAGCGACG AGAGCCTAGC GACCAATTTG 
AACTTGAAGA CTCTCATCTC GATTCAAGAA ACCGGCCCTT ACGGGAACAG ACCAACGCGC 
GTCAGACCTC ATCACAGGGA CGCCATGACC GCCAAATACA TCTTCGCTCA GGGTGGCCTC 
GATGATCAGA GCCTGGTCTC GTCTGTGACC AATCCGGCTT GCTTTGACCT CGCCGGCCGC 
ACGCTTTTGC ATCGCGCCGC GGATGACCCC AAGCGCATTG CACAGCTGCG ACAGTTGGTG 
GACGAATCGG GCCACGTCGA CGCGCCGCTT GCTGCCGGAT TGGACAATGC TGTTGGCGTC 
ACGGACATGT ACAATCGGTT CATCCACAGC GCCAAGCGCA GGGTAAGCGA TCGGTTGGGC 
GGGTTGAGCT TCCCGTCGCC CGTCTCGTCC GAGAGACGCA ATCAGGCGAC AGCCATGCAA 
ACCAGACCCA TGCTGCTCAC ACCTCAGGCC GAAGCCCCCG GGTCGACCGC ACTTCACATA 
GCGTGCACGA ATGACAACCA CGAGGCGGTG GCGTACCTGC TGGCCAAGGG CGCTGACGTG 
AACGCTATGA ATGACATAGG GCAAACCCCG TTGATGTTGG CGTGTCACTA CGGCAACCTG 
CTGGCGGTTT CGGCATTGGT TGCCACCGGG CGCGTCCTCA TCAACCTCCA GGACAATCAG 
CTCTTCGAAC TTCTCTTTGG TGCGGGCGCC AACGTCAACC AGCCCAACTA CAATGGATTC 
TCGGCGCTTC ACTTGGCCTG CATGGCCCGC AACCCTGCTC TCGTGGTCTA CCTGCTCTCG 
CGCGGCGCCT CGGTGAGTGT GGGGATCAGC CCGCTTTTCT GCCTGGCTGG CGATGGCGAG 
CTCTCGGTTT TCGCCACCCA GTCACGGGAT GTCGGCGTCT TGGTCGACGT TCTTGTCGAC 
GCTGGCTGCC CGGTCAACGC GGTCAACGAG ACGGGCAAAA CGTTGCTGCA CGTGGCCTGC 
CAGGACCGCG ACGCAGACTT GGCCGAGGCC CTGCTGCGCC ATGCCGACGT CGACGTAAAC 
TTGGCCGATG CTGTCGGATG GGCAGAGCGT TCGCTCCGTC TTCGATGCCG TCCCGCCGCG 
CCGCCCTCGC CGCCGCGACC GGGCCTCGCA TCCATGCTTC TCAGTCGACA GGCCAACGTC 
AATGCCACCG CCACTGACCC CCGACCAGAA GAGGACAAGA AGGAGAAAAG CAAGGTTGTC 
TCCTTCATGC GCAAGCTCAT CAAATCAGGC GCGGACGTCA ACGCCAAGGA CGCTCGAGGG 
TGGACCGCCC TGCACGCAGT GTGCGCTTCG AGCCACCTCG AAGCCGTTGG TTTGCTGTTG 
GCCCATGGCG CCAACGTCAA TGCCCGCGAT TTCAAGTACT CGCACGCTCA GCTCCTCGGC 
CCTAACAACT CGGAGTTTGA AGTGCGAGCA CCGGCGATAG TGGGACGGTC CGGACCAATC 
CAGAGGCGGG AACAACCACC TGCGAGTGAG GCCGACTCGT GGACCATTTG CAAGTTGCTC 
CTCGGCCACG GTGCTCAGTT CGATGTTGTC GATGAAGTCG AGGGCGAGAC TGCGCTGCAC 
TTGTTGACGC GCGCCGGCAC CGGACCACAA AACGGTCTGC TGGCCTGGGC TGTCGTGCAG 
CTCAAGGACC ACGGGGTCTT CCACCTGCGA AATGCCAAGG GCGAGACTCT GCTGCACAGT 
GCGATTGCTT GCAACAACTC CAAGCTGGTG TCGATTCTCT TGCAGCACGG CGCCGACAAG 
AGCCTCAGGT CCCACCTGGG CGAAACTGCG CTCCACACGG CACTGCGCCT GCGCCTTCAG 
AGTGCGACGC GTATTCGAAG TCTGTCCGAA CAGCCCGCCG AGTCCTGCGA TTCGGCCCTC 
TGGCCCACCT CGGCCCAGAT CTCGCCGCAC CAAGCCTATT GGCCCACCAG CGAGGTCAGC 
GAGCAGAACA TCGTGGCCCT CGTGGCTCGC GGGCTGACCG AAGAAGAGTG CACTGCGAGG 
GACTGCTTCG GCCTCACCGC GCTCCACTGG GCCGTCGCTT TGGGTGCCCT GCCCCTGCCC 
CACTTCCTCT TCCTGTCCCA CGCCGCCGCG TGGCTCGGAG CGAATGCCCC GAGGGCAACG 
CAGGACAAGA GCCTGTCGCG CCTTGCGGTC CTGTCGCTCT TTATCGTGAA CCACATCCTG 
ACTCACTTCA AGGACCGAGT GATGGATGCA GTGGATGAGC GTGGCGACAC GGCGCTACAC 
AGGCTGGCCG AAATGCGGCG GATCACTACT CTCATGGCTG TTTCTTCTCA GGCCAGTTTG 
ATCGTAAGAG CTCAGATGCC GACATCGACG ACGTCTTCCT GCGGGAGGCC TTCGGGGAGC 
TCACTTCGGC GGCGTCGCAG ATCGAGCAAC GCCTCTTTTC GCGGGAGGGC ACGACGACGA 
TCGAGGAGGA GCCGCTCCAC AAGCGGCGGA CCTCCCCCGC AGCAGCAGCA GCTGTTGTGG 
AGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_046640 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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