ACA1_024200 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_024200
Entrez Gene ID	14914146
Full Name	WD domain, G-beta repeat-containing protein
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004335557.1	XP_004335605.1	WD domain, G-beta repeat-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_024200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_024200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc103504	XM_004335557.1 Latest version!	Acanthamoeba castellanii str. Neff WD domain, G-beta repeat-containing protein (ACA1_024200) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_024200 ( XM_004335557.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004335605.1 Acanthamoeba castellanii str. Neff WD domain, G-beta repeat-containing protein (ACA1_024200) mRNA, complete cds.

Price & Availability↑

CloneID	OAc103504
Clone ID Related Accession (Same CDS sequence)	XM_004335557.1
Accession Version	XM_004335557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	WD domain, G-beta repeat-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457328). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGGAAGATA AAACAAATCT GCATCCTACA AGCACGAGCC GAAGCGAACC TGCCGACAGC 
GAGAAGCGAC CCCAAGAGAA GCTGCATAAA TCGGAGGAGA CCGGTCCGAG TCGCACCACC 
GCCGCCAATC CCAACGCTCG ATTCGAATTG ATCAACATCA AGAAAGATGA GGGGAACTTC 
CTGTTTAGGG AGAAAAAGTA CGCGGCGGCC CTCGAACGAT ACATCGAGGC GATGCTCGAG 
CTCAAGGTCT ACCGACGAGT CTGGCGAGAA AATCGCTTGC GCAAGATGGC CGCCGGCCAA 
GATGGCGCGC CCTCCACGTG CAGCCTCCAG ACCGAGCACG TTCCGACGAG CAAGGTGCTC 
GGGAAGCGGA ACTGCAACGG CAACGACAAG GGCAAGGAGA AGATGGAGCT CGAAGGTGAA 
GAGGTCGCGA CAGCGGAAGA GAAGTGCGAG AAGGAGGGCA ATGCCCCGAT GCTCGTGGAG 
GGAGAAGAGA CGACGCAGAC CTGCACCTCC TCGTCCGGCA AGTCTGCAGA GAGCACCGTA 
ACGTTGATCG TGTCGCCCGC GACCTGCACC ACCGCGCCGC CGTCTGCGGG GGACGACACG 
ATGGTGGACT GGGACGGTCT GCGGCGGAAG CGGCGACGCC TCGACGAGGC CGAGTGCAAG 
AGCGACGCTG CGGCCGACGA CATGACGGTG GAGATCATCG ACAAGTCGGA CTACACCATG 
CCCAAGGACA TGGGCTGCCA TGTCTCTTCC TCTTCCTCTT CCTCCTGTTG CTCCTCTTCC 
TCCTCCTCCT TTACTTCTTC GCTCGCGGTG ACTGCGCCGG TGGTTCCTGA TTGGGGCAAG 
CAGCGGTCCT TCAAGGACCT CACCGAGTGC GAGAAGAACC TGAAGCGGCT GGAGATCATC 
ATTCGGCTCA ACGTGGCCGC CTGCGGGATT CGGCTAGGCC GCGGCCAGCA GGCCCTCGAG 
CAATGCCGCA AGGTGGTCCG CATGAACGAC CTCGAGTGCT GCGAGCCGCT ACTCTATGCC 
AAAGCCCTCT ATCGGTATTT CTACACCTTT CGTGGTCGGA AAGCGCAAAG CCTGAAGATC 
ATGGGACAAA ACAAAGAAGC CTGCGCGTGC CTGCGCCTGG CGGCCACTTT CTCCCCCGCC 
GATCGCAGTA TTCGTAACGA ACTGTCCGCG CTCGAACAAG CGGTGAAGAA GGGGAGCGTC 
GTGTGCAGGC TTCTCGAAAA GCTGCGCTGC AAGAAGCAAG ACGACGCGGA AGATGAACCG 
CAGACGTCTC CAGCGCCGGA AGCGCCGAAG AGTAACAAAT GTACATTTTT CAGTGGACTC 
TTCGGCGGAA GCTGCTTGGC GCGGACCCCG CTTTCGCTGC TGTGCAGGAG GGAGATCTCC 
AGTCGGGGGC TCACACCGCA GCAGAAGGCC GATGTGGCCT GCCGACAGTT GCCCAAAGGG 
CCTCCGTCTC TTGTGGATAA GTACTGCCGC CGGGCCTACG CGGGCCAGTG GAGCAGCGAC 
GGCGATCTGC TCTTCACCAC CTGTCAGGAC TGGAAGATTC GCATCTACAC CCACGACTCG 
AAGACTCTCA CGCGGCGATG TATAATCAAT GCCAAGAAGG GGCAGTGGAC GATCACTGAC 
GCGCTGATGA GCAGCGACAA GACCTTCCTG CTGTACTCGT CGATCACGCC CCTCGTGCAC 
GTGGTGCCCC TGCCCCAGAA CCTGAACGAC GTGAACCCCA AAGGCGACCA TCCCGTTCTC 
AATTTTGTCG GGGCACAGGG CAGCGATTAT TCGGGCATCT GGTGCATGGC CCTCGCTCCC 
GACGACAGGG AGGTCCTGGC GGGGACGTCC GATAGTTCGG TGTGCGTGTA CGATCTGGAG 
AAGAACCAGC TCGTGGAGCG CGCGGCGGCC CATGGTGACG ACGTCAATGC CATTTGCTAC 
GTGGATACCG ACAACGGTGT CGGGTCCAGC GCACACCTCC TGGCTTCGGC GTCCGACGAC 
GGCCTCATCT ACCTGTGGGA CAAGCGCTGC ATGACCGAGC CCGTGGGGAC CCTCTTTGGT 
CACACCGAAG GTCTCACATC TTTGGCCTCA AAAGGCGATG GGCGATATCT GCTGTCCAAC 
AGCAAGGACC AGACCATGAA GCTCTGGGAC CTGCGGAGAA TGGTCGACCG CCACTCCGCC 
CAGCCGCCCG TGAAGAAGTA CGTGTGGGAC TACCGAGGCA TGAGCTACCC CGGCCATCCC 
GACGTCGACA GACATCCGTG CGACCTGTCG GTGTCCACCT TCCTGGGCCA CTCCGTGTTG 
CGGACGCTCA TCCGTTGTAA ATTTTCTCCC CCTGCTTGGT CGGGCCAGCG ATATGCGTGT 
ACGGCATCGG CGGACGGCAA GGTGTACATA TATGACGTGG CCTCCACGCA GCTCGTCCGC 
GTTTACGATC CCACGGGAGG CAACGAAACC ACCGCGAGTG CGATTGTGCG GGACCTGTCC 
TGGCACCCCT ACCTGCCCTA TCTTGTTACT GCGGCGTGGA ACGGAACGGT GTCGCTCTTC 
TCCCACGAAG CCAAGCCCCC ATCGGCCGAC GAAGGCGAGG AGGAGCGCAT GGTGCCGGAC 
CGCTCGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004335605.1
CDS	1..2592
Translation	MEDKTNLHPTSTSRSEPADSEKRPQEKLHKSEETGPSRTTAANPNARFELINIKKDEGNFLFREKKYAAALERYIEAMLELKVYRRVWRENRLRKMAAGQDGAPSTCSLQTEHVPTSKVLGKRNCNGNDKGKEKMELEGEEVATAEEKCEKEGNAPMLVEGEETTQTCTSSSGKSAESTVTLIVSPATCTTAPPSAGDDTMVDWDGLRRKRRRLDEAECKSDAAADDMTVEIIDKSDYTMPKDMGCHVSSSSSSSCCSSSSSSFTSSLAVTAPVVPDWGKQRSFKDLTECEKNLKRLEIIIRLNVAACGIRLGRGQQALEQCRKVVRMNDLECCEPLLYAKALYRYFYTFRGRKAQSLKIMGQNKEACACLRLAATFSPADRSIRNELSALEQAVKKGSVVCRLLEKLRCKKQDDAEDEPQTSPAPEAPKSNKCTFFSGLFGGSCLARTPLSLLCRREISSRGLTPQQKADVACRQLPKGPPSLVDKYCRRAYAGQWSSDGDLLFTTCQDWKIRIYTHDSKTLTRRCIINAKKGQWTITDALMSSDKTFLLYSSITPLVHVVPLPQNLNDVNPKGDHPVLNFVGAQGSDYSGIWCMALAPDDREVLAGTSDSSVCVYDLEKNQLVERAAAHGDDVNAICYVDTDNGVGSSAHLLASASDDGLIYLWDKRCMTEPVGTLFGHTEGLTSLASKGDGRYLLSNSKDQTMKLWDLRRMVDRHSAQPPVKKYVWDYRGMSYPGHPDVDRHPCDLSVSTFLGHSVLRTLIRCKFSPPAWSGQRYACTASADGKVYIYDVASTQLVRVYDPTGGNETTASAIVRDLSWHPYLPYLVTAAWNGTVSLFSHEAKPPSADEGEEERMVPDRSS

Target ORF information:

RefSeq Version	XM_004335557.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff WD domain, G-beta repeat-containing protein (ACA1_024200) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004335557.1

ORF Insert Sequence:

ATGGAAGATA AAACAAATCT GCATCCTACA AGCACGAGCC GAAGCGAACC TGCCGACAGC 
GAGAAGCGAC CCCAAGAGAA GCTGCATAAA TCGGAGGAGA CCGGTCCGAG TCGCACCACC 
GCCGCCAATC CCAACGCTCG ATTCGAATTG ATCAACATCA AGAAAGATGA GGGGAACTTC 
CTGTTTAGGG AGAAAAAGTA CGCGGCGGCC CTCGAACGAT ACATCGAGGC GATGCTCGAG 
CTCAAGGTCT ACCGACGAGT CTGGCGAGAA AATCGCTTGC GCAAGATGGC CGCCGGCCAA 
GATGGCGCGC CCTCCACGTG CAGCCTCCAG ACCGAGCACG TTCCGACGAG CAAGGTGCTC 
GGGAAGCGGA ACTGCAACGG CAACGACAAG GGCAAGGAGA AGATGGAGCT CGAAGGTGAA 
GAGGTCGCGA CAGCGGAAGA GAAGTGCGAG AAGGAGGGCA ATGCCCCGAT GCTCGTGGAG 
GGAGAAGAGA CGACGCAGAC CTGCACCTCC TCGTCCGGCA AGTCTGCAGA GAGCACCGTA 
ACGTTGATCG TGTCGCCCGC GACCTGCACC ACCGCGCCGC CGTCTGCGGG GGACGACACG 
ATGGTGGACT GGGACGGTCT GCGGCGGAAG CGGCGACGCC TCGACGAGGC CGAGTGCAAG 
AGCGACGCTG CGGCCGACGA CATGACGGTG GAGATCATCG ACAAGTCGGA CTACACCATG 
CCCAAGGACA TGGGCTGCCA TGTCTCTTCC TCTTCCTCTT CCTCCTGTTG CTCCTCTTCC 
TCCTCCTCCT TTACTTCTTC GCTCGCGGTG ACTGCGCCGG TGGTTCCTGA TTGGGGCAAG 
CAGCGGTCCT TCAAGGACCT CACCGAGTGC GAGAAGAACC TGAAGCGGCT GGAGATCATC 
ATTCGGCTCA ACGTGGCCGC CTGCGGGATT CGGCTAGGCC GCGGCCAGCA GGCCCTCGAG 
CAATGCCGCA AGGTGGTCCG CATGAACGAC CTCGAGTGCT GCGAGCCGCT ACTCTATGCC 
AAAGCCCTCT ATCGGTATTT CTACACCTTT CGTGGTCGGA AAGCGCAAAG CCTGAAGATC 
ATGGGACAAA ACAAAGAAGC CTGCGCGTGC CTGCGCCTGG CGGCCACTTT CTCCCCCGCC 
GATCGCAGTA TTCGTAACGA ACTGTCCGCG CTCGAACAAG CGGTGAAGAA GGGGAGCGTC 
GTGTGCAGGC TTCTCGAAAA GCTGCGCTGC AAGAAGCAAG ACGACGCGGA AGATGAACCG 
CAGACGTCTC CAGCGCCGGA AGCGCCGAAG AGTAACAAAT GTACATTTTT CAGTGGACTC 
TTCGGCGGAA GCTGCTTGGC GCGGACCCCG CTTTCGCTGC TGTGCAGGAG GGAGATCTCC 
AGTCGGGGGC TCACACCGCA GCAGAAGGCC GATGTGGCCT GCCGACAGTT GCCCAAAGGG 
CCTCCGTCTC TTGTGGATAA GTACTGCCGC CGGGCCTACG CGGGCCAGTG GAGCAGCGAC 
GGCGATCTGC TCTTCACCAC CTGTCAGGAC TGGAAGATTC GCATCTACAC CCACGACTCG 
AAGACTCTCA CGCGGCGATG TATAATCAAT GCCAAGAAGG GGCAGTGGAC GATCACTGAC 
GCGCTGATGA GCAGCGACAA GACCTTCCTG CTGTACTCGT CGATCACGCC CCTCGTGCAC 
GTGGTGCCCC TGCCCCAGAA CCTGAACGAC GTGAACCCCA AAGGCGACCA TCCCGTTCTC 
AATTTTGTCG GGGCACAGGG CAGCGATTAT TCGGGCATCT GGTGCATGGC CCTCGCTCCC 
GACGACAGGG AGGTCCTGGC GGGGACGTCC GATAGTTCGG TGTGCGTGTA CGATCTGGAG 
AAGAACCAGC TCGTGGAGCG CGCGGCGGCC CATGGTGACG ACGTCAATGC CATTTGCTAC 
GTGGATACCG ACAACGGTGT CGGGTCCAGC GCACACCTCC TGGCTTCGGC GTCCGACGAC 
GGCCTCATCT ACCTGTGGGA CAAGCGCTGC ATGACCGAGC CCGTGGGGAC CCTCTTTGGT 
CACACCGAAG GTCTCACATC TTTGGCCTCA AAAGGCGATG GGCGATATCT GCTGTCCAAC 
AGCAAGGACC AGACCATGAA GCTCTGGGAC CTGCGGAGAA TGGTCGACCG CCACTCCGCC 
CAGCCGCCCG TGAAGAAGTA CGTGTGGGAC TACCGAGGCA TGAGCTACCC CGGCCATCCC 
GACGTCGACA GACATCCGTG CGACCTGTCG GTGTCCACCT TCCTGGGCCA CTCCGTGTTG 
CGGACGCTCA TCCGTTGTAA ATTTTCTCCC CCTGCTTGGT CGGGCCAGCG ATATGCGTGT 
ACGGCATCGG CGGACGGCAA GGTGTACATA TATGACGTGG CCTCCACGCA GCTCGTCCGC 
GTTTACGATC CCACGGGAGG CAACGAAACC ACCGCGAGTG CGATTGTGCG GGACCTGTCC 
TGGCACCCCT ACCTGCCCTA TCTTGTTACT GCGGCGTGGA ACGGAACGGT GTCGCTCTTC 
TCCCACGAAG CCAAGCCCCC ATCGGCCGAC GAAGGCGAGG AGGAGCGCAT GGTGCCGGAC 
CGCTCGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_024200 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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