wwp1 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wwp1
Entrez Gene ID	102223052
Full Name	WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005808364.2	XP_005808421.1	NEDD4-like E3 ubiquitin-protein ligase WWP1
XM_005808364.3	XP_005808421.1	NEDD4-like E3 ubiquitin-protein ligase WWP1

Pathways from BioSystems
Gene Ontology

The following wwp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wwp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi13662	XM_005808364.3 Latest version!	Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OXi13662	XM_005808364.2	Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wwp1 ( XM_005808364.2 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005808421.1 Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Price & Availability↑

CloneID	OXi13662
Clone ID Related Accession (Same CDS sequence)	XM_005808364.2 , XM_005808364.3
Accession Version	XM_005808364.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Xiphophorus maculatus(Southern platyfish)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372400.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005808364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG CCTCATCGAG AGCAGAGACT AGCCATAACC ACAGACGCAC ATCACAGCTC 
CATGCCATTG TTTCATGTGC CAAACTCAAG AGGAAGAAGA GCGTTTTCGG GGCGGCCATT 
TACGTGGAGG TGACAGCAGA GGGAGAGACC CGCCGCACGG CAAAATCTCA CAGCTCCTCC 
AACCCGAAAT GGGACGAGAG GCTGACTCTG ACTGTAGCGC CACACACGCA GGTGGATTTC 
AAAGTATGGA GTCACCACAC CCTGAAAGCA GACGCTCTGC TGGGCAAAGC TACGCTGGAT 
CTCCTTCAGG CTTTGGAGCG GCATGACAGA AAATTGGAGA ATGTGAAGGA GGTGCTGAAG 
CTGAGTGTGG AACAGAAAGG GCTTTCTGTG CCTGTTGGCG AGCTGACTGT GTACCTTGAT 
GGCCTGACTG TCACTGACCA GGAGGAATTG GCGCCACTGA CCAATGGCAA CGCAGCAAAT 
GGCACCAAAG TCCAGCAGAA TGGTGATGCC ATTCATGAAA ACGGAGACTC CTCTTCCTCT 
TCTTCAAGGG CTGCAAACAG CACCGTGAAT GGAACAGACT TGGGTCAGAG GTCAGGCTCC 
TGCTCAGCCT CCAGCGGTGC AGATGGTCCG GTGCCTTCCA GCCCTGCTCT CGATCACACC 
ACCGGTATGA TCAACGGAGA TCCAACCCCC AACTCCACCC CAGTGCACCA ACCTCCAGAC 
AGCAGCCCAG ACACCAGAAT GGTAAACGGG GAATCCTCTG AGACTGTTCC CAGGCAATCT 
TCTGCTTCCA GAAGGGAAAC GCAGCCCTCT GCAGGTGAAA CTGAGGAGAG CTCTCACGAT 
GCTGCCCCGC CAAACGCTGA AACATCACCA GCCAGCACTT CCCGGCCCCC TCCGGCTCCC 
ACACCTTCCT CCGCCTCGTC TCCTGCTGCT AAACCTGCCA ACGGCGCCGC TTCCTCTTCC 
TCCTCCGCCG CCGCTGCTTC CACCACGTCA GCCTCTGCAA CCCAGGGAGA ACCCACCGTG 
ACGACGTCCT CATCGTTGTC CTCCTCCCCA GCGCCGGGAG AAGCGGGGGC TTCAGGAGCA 
GGCAGCAGTA GCAGCTCAAC AACAACAACA ACTGATGGGG TGAAACCCAG ACAACAGGTT 
TCTAATGCTC CAGCAGGAGA TCCTCTACCA CCAGGGTGGG AGCAGAGGAA AGATCCACAT 
GGGAGAACTT ACTATGTAGA CCACAACACC AGAACTACTA CTTGGGAAAG ACCACAGCCG 
CTTCCTCCAG GCTGGGAGCG CCGAGTGGAC GATCGGGGTC GGATCTATTA CGTGGACCAC 
AACACCCGGA CCACCACTTG GCAGCGGCCC ACCATGGAAT CGGTGCGCAA CTTTGAGCAG 
TGGCAGAGCC AGCGCAGCCA GCTGCAGGGA GCTATGCATC AGTTTAACCA GAGATACCTC 
TACTCTGCAT CTATGATGTC TGCTGAGAAT GACCCTCTGG GCCCTCTACC TCCTGGTTGG 
GAGAGGCGTG TGGACTCCAA TGACAGAGTT TACTTTGTGA ACCACAATAC CAAGACAACA 
CAGTGGGAAG ACCCTCGAAC CCAAGGGCTA CAGAATGAGG ATCCTCTGCC TGAAGGATGG 
GAAATCCGGT ACACGAGGGA AGGAGTTCGC TACTTTGTGG ATCACAACAC TCGGACGACC 
ACTTTCAGCG ACCCGCGCAC CGGGAAGTCT TCTGTCACCA AAGGCCCACA GATTGCATAC 
GAGCGCAGCT TCAGGTGGAA GCTCGCTCAC TTCCGTTACC TGTGTCAGTC TAACGCTCTG 
CCGAGCCACG TGAAAATCAC CGTCTCCAGA CAGACGCTGT TCGAAGACTC TTTTCAGCAA 
ATCATGGCTC TGAAGCCATA CGACCTACGG AGGAGGCTGT ACGTCATCTT CAGAGGCGAG 
GAGGGTCTGG ACTACGGTGG CTTAGCCAGG GAGTGGTTCT TCCTCTTGTC CCACGAGGTG 
CTGAACCCCA TGTACTGCCT GTTTGAGTAC GCAGGAAAGA GCAACTACTG TCTGCAGATC 
AACCCGGCGT CGGCCATCAA CCCAGATCAC CTCTCCTACT TCTGCTTCAT TGGCCGCTTC 
ATCGCGATGG CACTTTTCCA CGGGAAGTTC ATTGACACAG GCTTCTCTTT GCCGTTCTAC 
AAACGAATGC TGAACAAGAA GCTCATCCTG AAGGACCTGG AGTCCATCGA CCCGGAGTTC 
TACAACTCTC TCATCTGGAT TAGGGACAAC AACATCGAGG AATGTGGGCT GGAGATGTAC 
TTCTCCGTGG ACATGGAGAT CTTGGGGAAG ATCTCGTCTC ATGACCTGAA ACCCGATGGC 
GCCAACATCC TGGTGACGGA GGAGAACAAG GAGGAGTACA TCAGTCTGAT GGCAGAGTGG 
AGGTTCTCCC GTGGGGTGGA AGGTCAAACC AAAGCTTTTC TGGACGGCTT CAACGAAGTG 
GTCCCGCTTC AGTGGCTGCA GTATTTCGAT GAGAAGGAGC TGGAGGTGAT GCTGTGCGGC 
ATGCAGGAGG TGGACCTGCA GGACTGGCAG AGAAACACCG TGTACCGCCA CTACACCCGG 
AACAGCAAGC AGATCATCTG GTTCTGGCAG CTGGTGAAAG AAGTGGACAA CGAAGTGCGT 
CTGCGGCTCA TGCAGTTTGT GACGGGAACC TGCCGGCTTC CTCTGGGCGG CTTCGCTGAG 
CTGATGGGAA GCAACGGGCC GCAGAAGTTC TGCATCGAGA AAGTGGGGAA GGACACATGG 
CTTCCCCGTA GTCACACATG TTTTAACCGC CTGGACTTGC CTCCCTATAA AAGCTACGAA 
CAGCTGAAGG AGAAGCTGCT CTTCGCCATC GAGGAAACCG AAGGATTTGG CCAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005808421.1
CDS	139..3018
Translation	MATASSRAETSHNHRRTSQLHAIVSCAKLKRKKSVFGAAIYVEVTAEGETRRTAKSHSSSNPKWDERLTLTVAPHTQVDFKVWSHHTLKADALLGKATLDLLQALERHDRKLENVKEVLKLSVEQKGLSVPVGELTVYLDGLTVTDQEELAPLTNGNAANGTKVQQNGDAIHENGDSSSSSSRAANSTVNGTDLGQRSGSCSASSGADGPVPSSPALDHTTGMINGDPTPNSTPVHQPPDSSPDTRMVNGESSETVPRQSSASRRETQPSAGETEESSHDAAPPNAETSPASTSRPPPAPTPSSASSPAAKPANGAASSSSSAAAASTTSASATQGEPTVTTSSSLSSSPAPGEAGASGAGSSSSSTTTTTDGVKPRQQVSNAPAGDPLPPGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYSASMMSAENDPLGPLPPGWERRVDSNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSSVTKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASAINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKISSHDLKPDGANILVTEENKEEYISLMAEWRFSRGVEGQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_005808364.2
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005808364.2

ORF Insert Sequence:

ATGGCCACGG CCTCATCGAG AGCAGAGACT AGCCATAACC ACAGACGCAC ATCACAGCTC 
CATGCCATTG TTTCATGTGC CAAACTCAAG AGGAAGAAGA GCGTTTTCGG GGCGGCCATT 
TACGTGGAGG TGACAGCAGA GGGAGAGACC CGCCGCACGG CAAAATCTCA CAGCTCCTCC 
AACCCGAAAT GGGACGAGAG GCTGACTCTG ACTGTAGCGC CACACACGCA GGTGGATTTC 
AAAGTATGGA GTCACCACAC CCTGAAAGCA GACGCTCTGC TGGGCAAAGC TACGCTGGAT 
CTCCTTCAGG CTTTGGAGCG GCATGACAGA AAATTGGAGA ATGTGAAGGA GGTGCTGAAG 
CTGAGTGTGG AACAGAAAGG GCTTTCTGTG CCTGTTGGCG AGCTGACTGT GTACCTTGAT 
GGCCTGACTG TCACTGACCA GGAGGAATTG GCGCCACTGA CCAATGGCAA CGCAGCAAAT 
GGCACCAAAG TCCAGCAGAA TGGTGATGCC ATTCATGAAA ACGGAGACTC CTCTTCCTCT 
TCTTCAAGGG CTGCAAACAG CACCGTGAAT GGAACAGACT TGGGTCAGAG GTCAGGCTCC 
TGCTCAGCCT CCAGCGGTGC AGATGGTCCG GTGCCTTCCA GCCCTGCTCT CGATCACACC 
ACCGGTATGA TCAACGGAGA TCCAACCCCC AACTCCACCC CAGTGCACCA ACCTCCAGAC 
AGCAGCCCAG ACACCAGAAT GGTAAACGGG GAATCCTCTG AGACTGTTCC CAGGCAATCT 
TCTGCTTCCA GAAGGGAAAC GCAGCCCTCT GCAGGTGAAA CTGAGGAGAG CTCTCACGAT 
GCTGCCCCGC CAAACGCTGA AACATCACCA GCCAGCACTT CCCGGCCCCC TCCGGCTCCC 
ACACCTTCCT CCGCCTCGTC TCCTGCTGCT AAACCTGCCA ACGGCGCCGC TTCCTCTTCC 
TCCTCCGCCG CCGCTGCTTC CACCACGTCA GCCTCTGCAA CCCAGGGAGA ACCCACCGTG 
ACGACGTCCT CATCGTTGTC CTCCTCCCCA GCGCCGGGAG AAGCGGGGGC TTCAGGAGCA 
GGCAGCAGTA GCAGCTCAAC AACAACAACA ACTGATGGGG TGAAACCCAG ACAACAGGTT 
TCTAATGCTC CAGCAGGAGA TCCTCTACCA CCAGGGTGGG AGCAGAGGAA AGATCCACAT 
GGGAGAACTT ACTATGTAGA CCACAACACC AGAACTACTA CTTGGGAAAG ACCACAGCCG 
CTTCCTCCAG GCTGGGAGCG CCGAGTGGAC GATCGGGGTC GGATCTATTA CGTGGACCAC 
AACACCCGGA CCACCACTTG GCAGCGGCCC ACCATGGAAT CGGTGCGCAA CTTTGAGCAG 
TGGCAGAGCC AGCGCAGCCA GCTGCAGGGA GCTATGCATC AGTTTAACCA GAGATACCTC 
TACTCTGCAT CTATGATGTC TGCTGAGAAT GACCCTCTGG GCCCTCTACC TCCTGGTTGG 
GAGAGGCGTG TGGACTCCAA TGACAGAGTT TACTTTGTGA ACCACAATAC CAAGACAACA 
CAGTGGGAAG ACCCTCGAAC CCAAGGGCTA CAGAATGAGG ATCCTCTGCC TGAAGGATGG 
GAAATCCGGT ACACGAGGGA AGGAGTTCGC TACTTTGTGG ATCACAACAC TCGGACGACC 
ACTTTCAGCG ACCCGCGCAC CGGGAAGTCT TCTGTCACCA AAGGCCCACA GATTGCATAC 
GAGCGCAGCT TCAGGTGGAA GCTCGCTCAC TTCCGTTACC TGTGTCAGTC TAACGCTCTG 
CCGAGCCACG TGAAAATCAC CGTCTCCAGA CAGACGCTGT TCGAAGACTC TTTTCAGCAA 
ATCATGGCTC TGAAGCCATA CGACCTACGG AGGAGGCTGT ACGTCATCTT CAGAGGCGAG 
GAGGGTCTGG ACTACGGTGG CTTAGCCAGG GAGTGGTTCT TCCTCTTGTC CCACGAGGTG 
CTGAACCCCA TGTACTGCCT GTTTGAGTAC GCAGGAAAGA GCAACTACTG TCTGCAGATC 
AACCCGGCGT CGGCCATCAA CCCAGATCAC CTCTCCTACT TCTGCTTCAT TGGCCGCTTC 
ATCGCGATGG CACTTTTCCA CGGGAAGTTC ATTGACACAG GCTTCTCTTT GCCGTTCTAC 
AAACGAATGC TGAACAAGAA GCTCATCCTG AAGGACCTGG AGTCCATCGA CCCGGAGTTC 
TACAACTCTC TCATCTGGAT TAGGGACAAC AACATCGAGG AATGTGGGCT GGAGATGTAC 
TTCTCCGTGG ACATGGAGAT CTTGGGGAAG ATCTCGTCTC ATGACCTGAA ACCCGATGGC 
GCCAACATCC TGGTGACGGA GGAGAACAAG GAGGAGTACA TCAGTCTGAT GGCAGAGTGG 
AGGTTCTCCC GTGGGGTGGA AGGTCAAACC AAAGCTTTTC TGGACGGCTT CAACGAAGTG 
GTCCCGCTTC AGTGGCTGCA GTATTTCGAT GAGAAGGAGC TGGAGGTGAT GCTGTGCGGC 
ATGCAGGAGG TGGACCTGCA GGACTGGCAG AGAAACACCG TGTACCGCCA CTACACCCGG 
AACAGCAAGC AGATCATCTG GTTCTGGCAG CTGGTGAAAG AAGTGGACAA CGAAGTGCGT 
CTGCGGCTCA TGCAGTTTGT GACGGGAACC TGCCGGCTTC CTCTGGGCGG CTTCGCTGAG 
CTGATGGGAA GCAACGGGCC GCAGAAGTTC TGCATCGAGA AAGTGGGGAA GGACACATGG 
CTTCCCCGTA GTCACACATG TTTTAACCGC CTGGACTTGC CTCCCTATAA AAGCTACGAA 
CAGCTGAAGG AGAAGCTGCT CTTCGCCATC GAGGAAACCG AAGGATTTGG CCAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wwp1 ( XM_005808364.3 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005808421.1 Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Price & Availability↑

CloneID	OXi13662
Clone ID Related Accession (Same CDS sequence)	XM_005808364.2 , XM_005808364.3
Accession Version	XM_005808364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-21
Organism	Xiphophorus maculatus(southern platyfish)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 22, 2017 this sequence version replaced XM_005808364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG CCTCATCGAG AGCAGAGACT AGCCATAACC ACAGACGCAC ATCACAGCTC 
CATGCCATTG TTTCATGTGC CAAACTCAAG AGGAAGAAGA GCGTTTTCGG GGCGGCCATT 
TACGTGGAGG TGACAGCAGA GGGAGAGACC CGCCGCACGG CAAAATCTCA CAGCTCCTCC 
AACCCGAAAT GGGACGAGAG GCTGACTCTG ACTGTAGCGC CACACACGCA GGTGGATTTC 
AAAGTATGGA GTCACCACAC CCTGAAAGCA GACGCTCTGC TGGGCAAAGC TACGCTGGAT 
CTCCTTCAGG CTTTGGAGCG GCATGACAGA AAATTGGAGA ATGTGAAGGA GGTGCTGAAG 
CTGAGTGTGG AACAGAAAGG GCTTTCTGTG CCTGTTGGCG AGCTGACTGT GTACCTTGAT 
GGCCTGACTG TCACTGACCA GGAGGAATTG GCGCCACTGA CCAATGGCAA CGCAGCAAAT 
GGCACCAAAG TCCAGCAGAA TGGTGATGCC ATTCATGAAA ACGGAGACTC CTCTTCCTCT 
TCTTCAAGGG CTGCAAACAG CACCGTGAAT GGAACAGACT TGGGTCAGAG GTCAGGCTCC 
TGCTCAGCCT CCAGCGGTGC AGATGGTCCG GTGCCTTCCA GCCCTGCTCT CGATCACACC 
ACCGGTATGA TCAACGGAGA TCCAACCCCC AACTCCACCC CAGTGCACCA ACCTCCAGAC 
AGCAGCCCAG ACACCAGAAT GGTAAACGGG GAATCCTCTG AGACTGTTCC CAGGCAATCT 
TCTGCTTCCA GAAGGGAAAC GCAGCCCTCT GCAGGTGAAA CTGAGGAGAG CTCTCACGAT 
GCTGCCCCGC CAAACGCTGA AACATCACCA GCCAGCACTT CCCGGCCCCC TCCGGCTCCC 
ACACCTTCCT CCGCCTCGTC TCCTGCTGCT AAACCTGCCA ACGGCGCCGC TTCCTCTTCC 
TCCTCCGCCG CCGCTGCTTC CACCACGTCA GCCTCTGCAA CCCAGGGAGA ACCCACCGTG 
ACGACGTCCT CATCGTTGTC CTCCTCCCCA GCGCCGGGAG AAGCGGGGGC TTCAGGAGCA 
GGCAGCAGTA GCAGCTCAAC AACAACAACA ACTGATGGGG TGAAACCCAG ACAACAGGTT 
TCTAATGCTC CAGCAGGAGA TCCTCTACCA CCAGGGTGGG AGCAGAGGAA AGATCCACAT 
GGGAGAACTT ACTATGTAGA CCACAACACC AGAACTACTA CTTGGGAAAG ACCACAGCCG 
CTTCCTCCAG GCTGGGAGCG CCGAGTGGAC GATCGGGGTC GGATCTATTA CGTGGACCAC 
AACACCCGGA CCACCACTTG GCAGCGGCCC ACCATGGAAT CGGTGCGCAA CTTTGAGCAG 
TGGCAGAGCC AGCGCAGCCA GCTGCAGGGA GCTATGCATC AGTTTAACCA GAGATACCTC 
TACTCTGCAT CTATGATGTC TGCTGAGAAT GACCCTCTGG GCCCTCTACC TCCTGGTTGG 
GAGAGGCGTG TGGACTCCAA TGACAGAGTT TACTTTGTGA ACCACAATAC CAAGACAACA 
CAGTGGGAAG ACCCTCGAAC CCAAGGGCTA CAGAATGAGG ATCCTCTGCC TGAAGGATGG 
GAAATCCGGT ACACGAGGGA AGGAGTTCGC TACTTTGTGG ATCACAACAC TCGGACGACC 
ACTTTCAGCG ACCCGCGCAC CGGGAAGTCT TCTGTCACCA AAGGCCCACA GATTGCATAC 
GAGCGCAGCT TCAGGTGGAA GCTCGCTCAC TTCCGTTACC TGTGTCAGTC TAACGCTCTG 
CCGAGCCACG TGAAAATCAC CGTCTCCAGA CAGACGCTGT TCGAAGACTC TTTTCAGCAA 
ATCATGGCTC TGAAGCCATA CGACCTACGG AGGAGGCTGT ACGTCATCTT CAGAGGCGAG 
GAGGGTCTGG ACTACGGTGG CTTAGCCAGG GAGTGGTTCT TCCTCTTGTC CCACGAGGTG 
CTGAACCCCA TGTACTGCCT GTTTGAGTAC GCAGGAAAGA GCAACTACTG TCTGCAGATC 
AACCCGGCGT CGGCCATCAA CCCAGATCAC CTCTCCTACT TCTGCTTCAT TGGCCGCTTC 
ATCGCGATGG CACTTTTCCA CGGGAAGTTC ATTGACACAG GCTTCTCTTT GCCGTTCTAC 
AAACGAATGC TGAACAAGAA GCTCATCCTG AAGGACCTGG AGTCCATCGA CCCGGAGTTC 
TACAACTCTC TCATCTGGAT TAGGGACAAC AACATCGAGG AATGTGGGCT GGAGATGTAC 
TTCTCCGTGG ACATGGAGAT CTTGGGGAAG ATCTCGTCTC ATGACCTGAA ACCCGATGGC 
GCCAACATCC TGGTGACGGA GGAGAACAAG GAGGAGTACA TCAGTCTGAT GGCAGAGTGG 
AGGTTCTCCC GTGGGGTGGA AGGTCAAACC AAAGCTTTTC TGGACGGCTT CAACGAAGTG 
GTCCCGCTTC AGTGGCTGCA GTATTTCGAT GAGAAGGAGC TGGAGGTGAT GCTGTGCGGC 
ATGCAGGAGG TGGACCTGCA GGACTGGCAG AGAAACACCG TGTACCGCCA CTACACCCGG 
AACAGCAAGC AGATCATCTG GTTCTGGCAG CTGGTGAAAG AAGTGGACAA CGAAGTGCGT 
CTGCGGCTCA TGCAGTTTGT GACGGGAACC TGCCGGCTTC CTCTGGGCGG CTTCGCTGAG 
CTGATGGGAA GCAACGGGCC GCAGAAGTTC TGCATCGAGA AAGTGGGGAA GGACACATGG 
CTTCCCCGTA GTCACACATG TTTTAACCGC CTGGACTTGC CTCCCTATAA AAGCTACGAA 
CAGCTGAAGG AGAAGCTGCT CTTCGCCATC GAGGAAACCG AAGGATTTGG CCAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005808421.1
CDS	230..3109
Translation	MATASSRAETSHNHRRTSQLHAIVSCAKLKRKKSVFGAAIYVEVTAEGETRRTAKSHSSSNPKWDERLTLTVAPHTQVDFKVWSHHTLKADALLGKATLDLLQALERHDRKLENVKEVLKLSVEQKGLSVPVGELTVYLDGLTVTDQEELAPLTNGNAANGTKVQQNGDAIHENGDSSSSSSRAANSTVNGTDLGQRSGSCSASSGADGPVPSSPALDHTTGMINGDPTPNSTPVHQPPDSSPDTRMVNGESSETVPRQSSASRRETQPSAGETEESSHDAAPPNAETSPASTSRPPPAPTPSSASSPAAKPANGAASSSSSAAAASTTSASATQGEPTVTTSSSLSSSPAPGEAGASGAGSSSSSTTTTTDGVKPRQQVSNAPAGDPLPPGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRIYYVDHNTRTTTWQRPTMESVRNFEQWQSQRSQLQGAMHQFNQRYLYSASMMSAENDPLGPLPPGWERRVDSNDRVYFVNHNTKTTQWEDPRTQGLQNEDPLPEGWEIRYTREGVRYFVDHNTRTTTFSDPRTGKSSVTKGPQIAYERSFRWKLAHFRYLCQSNALPSHVKITVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKSNYCLQINPASAINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKKLILKDLESIDPEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKISSHDLKPDGANILVTEENKEEYISLMAEWRFSRGVEGQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLQDWQRNTVYRHYTRNSKQIIWFWQLVKEVDNEVRLRLMQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_005808364.3
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus WW domain containing E3 ubiquitin protein ligase 1 (wwp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005808364.3

ORF Insert Sequence:

ATGGCCACGG CCTCATCGAG AGCAGAGACT AGCCATAACC ACAGACGCAC ATCACAGCTC 
CATGCCATTG TTTCATGTGC CAAACTCAAG AGGAAGAAGA GCGTTTTCGG GGCGGCCATT 
TACGTGGAGG TGACAGCAGA GGGAGAGACC CGCCGCACGG CAAAATCTCA CAGCTCCTCC 
AACCCGAAAT GGGACGAGAG GCTGACTCTG ACTGTAGCGC CACACACGCA GGTGGATTTC 
AAAGTATGGA GTCACCACAC CCTGAAAGCA GACGCTCTGC TGGGCAAAGC TACGCTGGAT 
CTCCTTCAGG CTTTGGAGCG GCATGACAGA AAATTGGAGA ATGTGAAGGA GGTGCTGAAG 
CTGAGTGTGG AACAGAAAGG GCTTTCTGTG CCTGTTGGCG AGCTGACTGT GTACCTTGAT 
GGCCTGACTG TCACTGACCA GGAGGAATTG GCGCCACTGA CCAATGGCAA CGCAGCAAAT 
GGCACCAAAG TCCAGCAGAA TGGTGATGCC ATTCATGAAA ACGGAGACTC CTCTTCCTCT 
TCTTCAAGGG CTGCAAACAG CACCGTGAAT GGAACAGACT TGGGTCAGAG GTCAGGCTCC 
TGCTCAGCCT CCAGCGGTGC AGATGGTCCG GTGCCTTCCA GCCCTGCTCT CGATCACACC 
ACCGGTATGA TCAACGGAGA TCCAACCCCC AACTCCACCC CAGTGCACCA ACCTCCAGAC 
AGCAGCCCAG ACACCAGAAT GGTAAACGGG GAATCCTCTG AGACTGTTCC CAGGCAATCT 
TCTGCTTCCA GAAGGGAAAC GCAGCCCTCT GCAGGTGAAA CTGAGGAGAG CTCTCACGAT 
GCTGCCCCGC CAAACGCTGA AACATCACCA GCCAGCACTT CCCGGCCCCC TCCGGCTCCC 
ACACCTTCCT CCGCCTCGTC TCCTGCTGCT AAACCTGCCA ACGGCGCCGC TTCCTCTTCC 
TCCTCCGCCG CCGCTGCTTC CACCACGTCA GCCTCTGCAA CCCAGGGAGA ACCCACCGTG 
ACGACGTCCT CATCGTTGTC CTCCTCCCCA GCGCCGGGAG AAGCGGGGGC TTCAGGAGCA 
GGCAGCAGTA GCAGCTCAAC AACAACAACA ACTGATGGGG TGAAACCCAG ACAACAGGTT 
TCTAATGCTC CAGCAGGAGA TCCTCTACCA CCAGGGTGGG AGCAGAGGAA AGATCCACAT 
GGGAGAACTT ACTATGTAGA CCACAACACC AGAACTACTA CTTGGGAAAG ACCACAGCCG 
CTTCCTCCAG GCTGGGAGCG CCGAGTGGAC GATCGGGGTC GGATCTATTA CGTGGACCAC 
AACACCCGGA CCACCACTTG GCAGCGGCCC ACCATGGAAT CGGTGCGCAA CTTTGAGCAG 
TGGCAGAGCC AGCGCAGCCA GCTGCAGGGA GCTATGCATC AGTTTAACCA GAGATACCTC 
TACTCTGCAT CTATGATGTC TGCTGAGAAT GACCCTCTGG GCCCTCTACC TCCTGGTTGG 
GAGAGGCGTG TGGACTCCAA TGACAGAGTT TACTTTGTGA ACCACAATAC CAAGACAACA 
CAGTGGGAAG ACCCTCGAAC CCAAGGGCTA CAGAATGAGG ATCCTCTGCC TGAAGGATGG 
GAAATCCGGT ACACGAGGGA AGGAGTTCGC TACTTTGTGG ATCACAACAC TCGGACGACC 
ACTTTCAGCG ACCCGCGCAC CGGGAAGTCT TCTGTCACCA AAGGCCCACA GATTGCATAC 
GAGCGCAGCT TCAGGTGGAA GCTCGCTCAC TTCCGTTACC TGTGTCAGTC TAACGCTCTG 
CCGAGCCACG TGAAAATCAC CGTCTCCAGA CAGACGCTGT TCGAAGACTC TTTTCAGCAA 
ATCATGGCTC TGAAGCCATA CGACCTACGG AGGAGGCTGT ACGTCATCTT CAGAGGCGAG 
GAGGGTCTGG ACTACGGTGG CTTAGCCAGG GAGTGGTTCT TCCTCTTGTC CCACGAGGTG 
CTGAACCCCA TGTACTGCCT GTTTGAGTAC GCAGGAAAGA GCAACTACTG TCTGCAGATC 
AACCCGGCGT CGGCCATCAA CCCAGATCAC CTCTCCTACT TCTGCTTCAT TGGCCGCTTC 
ATCGCGATGG CACTTTTCCA CGGGAAGTTC ATTGACACAG GCTTCTCTTT GCCGTTCTAC 
AAACGAATGC TGAACAAGAA GCTCATCCTG AAGGACCTGG AGTCCATCGA CCCGGAGTTC 
TACAACTCTC TCATCTGGAT TAGGGACAAC AACATCGAGG AATGTGGGCT GGAGATGTAC 
TTCTCCGTGG ACATGGAGAT CTTGGGGAAG ATCTCGTCTC ATGACCTGAA ACCCGATGGC 
GCCAACATCC TGGTGACGGA GGAGAACAAG GAGGAGTACA TCAGTCTGAT GGCAGAGTGG 
AGGTTCTCCC GTGGGGTGGA AGGTCAAACC AAAGCTTTTC TGGACGGCTT CAACGAAGTG 
GTCCCGCTTC AGTGGCTGCA GTATTTCGAT GAGAAGGAGC TGGAGGTGAT GCTGTGCGGC 
ATGCAGGAGG TGGACCTGCA GGACTGGCAG AGAAACACCG TGTACCGCCA CTACACCCGG 
AACAGCAAGC AGATCATCTG GTTCTGGCAG CTGGTGAAAG AAGTGGACAA CGAAGTGCGT 
CTGCGGCTCA TGCAGTTTGT GACGGGAACC TGCCGGCTTC CTCTGGGCGG CTTCGCTGAG 
CTGATGGGAA GCAACGGGCC GCAGAAGTTC TGCATCGAGA AAGTGGGGAA GGACACATGG 
CTTCCCCGTA GTCACACATG TTTTAACCGC CTGGACTTGC CTCCCTATAA AAGCTACGAA 
CAGCTGAAGG AGAAGCTGCT CTTCGCCATC GAGGAAACCG AAGGATTTGG CCAAGAATAG