chi cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	chi
Entrez Gene ID	100136076
Full Name	myeloid cell lineage chitinase
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124383.1	NP_001117855.1	chitinase precursor

Pathways from BioSystems
Gene Ontology

The following chi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00989	NM_001124383.1 Latest version!	Oncorhynchus mykiss myeloid cell lineage chitinase (chi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chi ( NM_001124383.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117855.1 Oncorhynchus mykiss myeloid cell lineage chitinase (chi), mRNA.

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CloneID	OOf00989
Clone ID Related Accession (Same CDS sequence)	NM_001124383.1
Accession Version	NM_001124383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Oncorhynchus mykiss(rainbow trout)
Product	chitinase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ535688.1. ##Evidence-Data-START## Transcript exon combination :: AJ535688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06640870, SAMN06640877 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAAGC TCACTTTACT TGCAGGTCTG TGCTTCGCCC TGTGCCAATT GGGCTCGACC 
TCCCAACTGG TCTGCTACTT TACCAACTGG TCCCAGTACA GACCTGGAAC TGGGAAGTTC 
CTGCCAGCTA ATGTTGACCC TCACCTGTGT ACTCACCTGA TCTACGCCTT CTCCATTATC 
AACCCTGCCA ATGAGCTTGC CACCTATGAG TGGAATGATG AGACCCTCTA CAGTTCCTTC 
AATGGACTTA AAGACAGAAA TCCTCAGCTC AAAACACTTT TGGCTGTCGG TGGATGGAAG 
TTTGGCACAC AACAGTTCAG TATCATGGTT TCATCACCTG ACAATCGGCT GAAGTTCATC 
CAGTCTTCGA TCAGTTTCCT TCGGACGCAT AGTTTTGACG GGCTGGACCT GGACTGGGAG 
TACCCTGGAG CACGGGGCAG CCCTCCAGAG GACAAGCAGA GATTCACACT GTTGTGCAAG 
GAGCTCCTCG AGGCCTTTGA GGCTGAGGGC AAGGCAGTCA GCCGCCCCAG GCTGCTGCTT 
ACGGCTGCCG TGGCTGCTGG GAAAGGGACC ATTGATTCTG GCTATGAAAT TGCAGAAATT 
GCTAAATACC TGGACTTCAT TAGCGTGATG ACCTATGACT TCCATGGATC CTGGGAGACC 
TTCACAGGAC ACAACAGCCC TCTCTACCAG GGATCCCATG ACACAGGAGA CCATATCTAC 
TTGAACACAG ATTTTGCCAT GAAATACTGG CGAGATCAGG GAGCCCCTGT TGAGAAGCTG 
ATGATGGGAT TTGCTACGTA TGGACGTTCG TTCCAGCTCG CCTCTGTAGA TAGTGGGGTC 
GGAGCGTCAG CCAACGGTGC TGCTGCAGCT GGACCTTTCA CCAGGGAAGC TGGTTTCTGG 
TCTTATTATG AGATCTGTAC TTTCCTCCAA GGAGCCAGTT TCCAGTGGAT TGAGGATCAA 
AAGGTTCCCT ATGCCTCTAA AGGAAATCAG TGGGTAGGAT TTGACAATAG GGAGAGCTAT 
GACACAAAGG TCGGCTACCT TAAGGAAAAT GGCTTTGGAG GGGCCATGGT TTGGAATCTG 
GACCTGGATG ACTTTGCTGG ACAGTCATGT GGACAGGGCA ACTATCCCCT GATCAGCCAT 
CTTCAAAAAC TCCTCAACAT TGAACGACCT CCCCTGCCAC CTACACACAC CCCAATGCCA 
GGGGAACCAC CCACAGTCAA GGCCACAACT AAGGCCAGTG GTAGTTTCTG TGCAGGCAGG 
GCTGATGGGC TGTATGTCAA AGCAGATTCC CCGAGCTCTT TCTATAACTG TGCTAACGGC 
ATCACCTGGA TCCAGAGCTG CCCCGCTGGC CTCGTCTTCA GTGACAGCTG CAAATGCTGC 
AACTGGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117855.1
CDS	39..1430
Translation	MSKLTLLAGLCFALCQLGSTSQLVCYFTNWSQYRPGTGKFLPANVDPHLCTHLIYAFSIINPANELATYEWNDETLYSSFNGLKDRNPQLKTLLAVGGWKFGTQQFSIMVSSPDNRLKFIQSSISFLRTHSFDGLDLDWEYPGARGSPPEDKQRFTLLCKELLEAFEAEGKAVSRPRLLLTAAVAAGKGTIDSGYEIAEIAKYLDFISVMTYDFHGSWETFTGHNSPLYQGSHDTGDHIYLNTDFAMKYWRDQGAPVEKLMMGFATYGRSFQLASVDSGVGASANGAAAAGPFTREAGFWSYYEICTFLQGASFQWIEDQKVPYASKGNQWVGFDNRESYDTKVGYLKENGFGGAMVWNLDLDDFAGQSCGQGNYPLISHLQKLLNIERPPLPPTHTPMPGEPPTVKATTKASGSFCAGRADGLYVKADSPSSFYNCANGITWIQSCPAGLVFSDSCKCCNWP

Target ORF information:

RefSeq Version	NM_001124383.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss myeloid cell lineage chitinase (chi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124383.1

ORF Insert Sequence:

ATGAGCAAGC TCACTTTACT TGCAGGTCTG TGCTTCGCCC TGTGCCAATT GGGCTCGACC 
TCCCAACTGG TCTGCTACTT TACCAACTGG TCCCAGTACA GACCTGGAAC TGGGAAGTTC 
CTGCCAGCTA ATGTTGACCC TCACCTGTGT ACTCACCTGA TCTACGCCTT CTCCATTATC 
AACCCTGCCA ATGAGCTTGC CACCTATGAG TGGAATGATG AGACCCTCTA CAGTTCCTTC 
AATGGACTTA AAGACAGAAA TCCTCAGCTC AAAACACTTT TGGCTGTCGG TGGATGGAAG 
TTTGGCACAC AACAGTTCAG TATCATGGTT TCATCACCTG ACAATCGGCT GAAGTTCATC 
CAGTCTTCGA TCAGTTTCCT TCGGACGCAT AGTTTTGACG GGCTGGACCT GGACTGGGAG 
TACCCTGGAG CACGGGGCAG CCCTCCAGAG GACAAGCAGA GATTCACACT GTTGTGCAAG 
GAGCTCCTCG AGGCCTTTGA GGCTGAGGGC AAGGCAGTCA GCCGCCCCAG GCTGCTGCTT 
ACGGCTGCCG TGGCTGCTGG GAAAGGGACC ATTGATTCTG GCTATGAAAT TGCAGAAATT 
GCTAAATACC TGGACTTCAT TAGCGTGATG ACCTATGACT TCCATGGATC CTGGGAGACC 
TTCACAGGAC ACAACAGCCC TCTCTACCAG GGATCCCATG ACACAGGAGA CCATATCTAC 
TTGAACACAG ATTTTGCCAT GAAATACTGG CGAGATCAGG GAGCCCCTGT TGAGAAGCTG 
ATGATGGGAT TTGCTACGTA TGGACGTTCG TTCCAGCTCG CCTCTGTAGA TAGTGGGGTC 
GGAGCGTCAG CCAACGGTGC TGCTGCAGCT GGACCTTTCA CCAGGGAAGC TGGTTTCTGG 
TCTTATTATG AGATCTGTAC TTTCCTCCAA GGAGCCAGTT TCCAGTGGAT TGAGGATCAA 
AAGGTTCCCT ATGCCTCTAA AGGAAATCAG TGGGTAGGAT TTGACAATAG GGAGAGCTAT 
GACACAAAGG TCGGCTACCT TAAGGAAAAT GGCTTTGGAG GGGCCATGGT TTGGAATCTG 
GACCTGGATG ACTTTGCTGG ACAGTCATGT GGACAGGGCA ACTATCCCCT GATCAGCCAT 
CTTCAAAAAC TCCTCAACAT TGAACGACCT CCCCTGCCAC CTACACACAC CCCAATGCCA 
GGGGAACCAC CCACAGTCAA GGCCACAACT AAGGCCAGTG GTAGTTTCTG TGCAGGCAGG 
GCTGATGGGC TGTATGTCAA AGCAGATTCC CCGAGCTCTT TCTATAACTG TGCTAACGGC 
ATCACCTGGA TCCAGAGCTG CCCCGCTGGC CTCGTCTTCA GTGACAGCTG CAAATGCTGC 
AACTGGCCCT AG