LOC25484582 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25484582
Entrez Gene ID	25484582
Full Name	heavy metal transport/detoxification superfamily protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013613433.1	XP_013468887.1	heavy metal transport/detoxification superfamily protein
XM_024781516.1	XP_024637284.1	heavy metal-associated isoprenylated plant protein 30 isoform X2
XM_013613433.2	XP_013468887.1	heavy metal-associated isoprenylated plant protein 30 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25484582 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25484582 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe212221	XM_013613433.2 Latest version!	Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OMe257946	XM_024781516.1 Latest version!	Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMe212221	XM_013613433.1	Medicago truncatula heavy metal transport/detoxification superfamily protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25484582 ( XM_013613433.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468887.1 Medicago truncatula heavy metal transport/detoxification superfamily protein mRNA.

Price & Availability↑

CloneID	OMe212221
Clone ID Related Accession (Same CDS sequence)	XM_013613433.1 , XM_013613433.2
Accession Version	XM_013613433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	heavy metal transport/detoxification superfamily protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAAGTT AGTAACATGT TTAATGATGA TAATGTTCAT 
GCTTGCTCTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468887.1
CDS	94..651
Translation	MASLLYRTFGYQISSFVNRWFIQPSHHHNHSHNNNHKTNKRFNMPKGRPISLQTVELKVRMCCTGCERVVKNAIYKLKGIDSVKVDLEMERVTVTGYVERNKVLKAVRRSGKRAEFWPYPNPPLYFTSASHYYKDTTNEFKESYNYYRHGYNLPDRHGTIHVTQRGDDQVSNMFNDDNVHACSLM

Target ORF information:

RefSeq Version	XM_013613433.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula heavy metal transport/detoxification superfamily protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613433.1

ORF Insert Sequence:

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAAGTT AGTAACATGT TTAATGATGA TAATGTTCAT 
GCTTGCTCTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25484582 ( XM_013613433.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468887.1 Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe212221
Clone ID Related Accession (Same CDS sequence)	XM_013613433.1 , XM_013613433.2
Accession Version	XM_013613433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	heavy metal-associated isoprenylated plant protein 30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013613433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAAGTT AGTAACATGT TTAATGATGA TAATGTTCAT 
GCTTGCTCTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468887.1
CDS	117..674
Translation	MASLLYRTFGYQISSFVNRWFIQPSHHHNHSHNNNHKTNKRFNMPKGRPISLQTVELKVRMCCTGCERVVKNAIYKLKGIDSVKVDLEMERVTVTGYVERNKVLKAVRRSGKRAEFWPYPNPPLYFTSASHYYKDTTNEFKESYNYYRHGYNLPDRHGTIHVTQRGDDQVSNMFNDDNVHACSLM

Target ORF information:

RefSeq Version	XM_013613433.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613433.2

ORF Insert Sequence:

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAAGTT AGTAACATGT TTAATGATGA TAATGTTCAT 
GCTTGCTCTC TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25484582 ( XM_024781516.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024637284.1 Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe257946
Clone ID Related Accession (Same CDS sequence)	XM_024781516.1
Accession Version	XM_024781516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	heavy metal-associated isoprenylated plant protein 30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAATGT GTATTATATG CTCATTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024637284.1
CDS	117..650
Translation	MASLLYRTFGYQISSFVNRWFIQPSHHHNHSHNNNHKTNKRFNMPKGRPISLQTVELKVRMCCTGCERVVKNAIYKLKGIDSVKVDLEMERVTVTGYVERNKVLKAVRRSGKRAEFWPYPNPPLYFTSASHYYKDTTNEFKESYNYYRHGYNLPDRHGTIHVTQRGDDQCVLYAHYR

Target ORF information:

RefSeq Version	XM_024781516.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula heavy metal-associated isoprenylated plant protein 30 (LOC25484582), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024781516.1

ORF Insert Sequence:

ATGGCTTCCT TATTATATAG AACTTTTGGC TATCAAATCT CTTCATTTGT AAACCGTTGG 
TTTATACAAC CATCTCATCA TCATAATCAT AGTCATAATA ATAATCACAA AACCAATAAG 
AGGTTCAACA TGCCGAAGGG TCGACCGATT TCCTTACAGA CTGTTGAGCT GAAAGTAAGG 
ATGTGTTGTA CAGGTTGTGA AAGAGTTGTT AAAAACGCTA TCTACAAGCT TAAAGGCATA 
GATTCGGTGA AAGTGGACTT AGAGATGGAA AGGGTAACAG TTACAGGATA TGTTGAGAGG 
AACAAAGTGC TTAAAGCAGT TAGGAGATCA GGAAAAAGAG CTGAATTTTG GCCATACCCA 
AATCCACCAC TTTACTTCAC ATCAGCTAGT CATTACTACA AAGATACAAC CAATGAGTTC 
AAAGAAAGTT ACAATTACTA TAGACATGGT TACAATCTCC CTGATAGGCA TGGAACCATT 
CATGTTACTC AACGTGGAGA TGACCAATGT GTATTATATG CTCATTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25484582 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム