LOC25481978 cDNA ORF clone, Medicago truncatula(barrel medic)

The following LOC25481978 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25481978 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe208729 XM_013610405.1
Latest version!
Medicago truncatula nodulin MtN21/EamA-like transporter family protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe208729
    Clone ID Related Accession (Same CDS sequence) XM_013610405.1
    Accession Version XM_013610405.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-08-24
    Organism Medicago truncatula(barrel medic)
    Product nodulin MtN21/EamA-like transporter family protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACTCAGT TATTATGGGA CGGAGGAAAT ATTATCTTTG GTTTAACAAT AGCAGTAGCT 
    TTTATAAATG AAACCGACAA AAGAAACACA CTGAAATCAA GAGAACTGAG AAAAGTTGTA
    GCTCAGCTTT TTGGATATAA AGGACTTGAA TACACAACAC CTACTCTTGC TTCATCTCTT
    TCCAACCTTA TACCTGCTTT TACATTCATC CTAGCTATCA TTTTCAGGAT GGAGAAGGTA
    AAATTAAGAT GGAAAAGCAG TCAAGCAAAA ATCTTGGGTT CTACGGTATC AATATTAGGT
    GCACTCCTAG TTGTTCTTTA TAAGGGTCCA ATAATCATAC CCTCACCATC AACACAATCA
    CCACCTATTA TTCATTCTCC AATCACTTCT TCAACAACAG AATCAAATTG GATTCTTGGT
    GGATCTCTAC TTGTTATTGA GATTCTTATT GTTCCAATAT GGTACATTAT TCAGGTCAGA
    GAGAAGCACT TACATGAATA CGTTGAATTG GTTCAAGCAA AAATGAAAGA GTTCGACTCT
    ATTGAAATAC AACATGTTTC CGTGAACATA GCGCTCGTGA TGAGAGGAAA AAGGAAAATG
    TGGATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013465859.1
    CDS1..609
    Translation

    Target ORF information:

    RefSeq Version XM_013610405.1
    Organism Medicago truncatula(barrel medic)
    Definition Medicago truncatula nodulin MtN21/EamA-like transporter family protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013610405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACTCAGT TATTATGGGA CGGAGGAAAT ATTATCTTTG GTTTAACAAT AGCAGTAGCT 
    TTTATAAATG AAACCGACAA AAGAAACACA CTGAAATCAA GAGAACTGAG AAAAGTTGTA
    GCTCAGCTTT TTGGATATAA AGGACTTGAA TACACAACAC CTACTCTTGC TTCATCTCTT
    TCCAACCTTA TACCTGCTTT TACATTCATC CTAGCTATCA TTTTCAGGAT GGAGAAGGTA
    AAATTAAGAT GGAAAAGCAG TCAAGCAAAA ATCTTGGGTT CTACGGTATC AATATTAGGT
    GCACTCCTAG TTGTTCTTTA TAAGGGTCCA ATAATCATAC CCTCACCATC AACACAATCA
    CCACCTATTA TTCATTCTCC AATCACTTCT TCAACAACAG AATCAAATTG GATTCTTGGT
    GGATCTCTAC TTGTTATTGA GATTCTTATT GTTCCAATAT GGTACATTAT TCAGGTCAGA
    GAGAAGCACT TACATGAATA CGTTGAATTG GTTCAAGCAA AAATGAAAGA GTTCGACTCT
    ATTGAAATAC AACATGTTTC CGTGAACATA GCGCTCGTGA TGAGAGGAAA AAGGAAAATG
    TGGATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC genomics15312(2014 Apr)
    Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD


    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature480(7378)520-4(2011 Nov)
    Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA