LOC11423130 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11423130
Entrez Gene ID	11423130
Full Name	Non-lysosomal glucosylceramidase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013613154.1	XP_013468608.1	Non-lysosomal glucosylceramidase
XM_003590653.3	XP_003590701.2	non-lysosomal glucosylceramidase isoform X1
XM_003590653.2	XP_003590701.2	Non-lysosomal glucosylceramidase
XM_013613154.2	XP_013468608.1	non-lysosomal glucosylceramidase isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11423130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11423130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe207899	XM_003590653.3 Latest version!	Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe211933	XM_013613154.2 Latest version!	Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe207899	XM_003590653.2	Medicago truncatula Non-lysosomal glucosylceramidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe211933	XM_013613154.1	Medicago truncatula Non-lysosomal glucosylceramidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11423130 ( XM_003590653.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003590701.2 Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe207899
Clone ID Related Accession (Same CDS sequence)	XM_003590653.3 , XM_003590653.2
Accession Version	XM_003590653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	non-lysosomal glucosylceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003590653.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTGTGAAAA AGATTGCAAC GAAAACAAAA 
TGCTTTAGCA GTTCTGTGTT TAATTGTGCT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003590701.2
CDS	400..3375
Translation	MRDFERMVSGNLFHSRKNSWPTEEYINKTTLQLFDIDNAAPPEQAWRRRLNSHANLLKEFRVTFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFVSREGGNKSFASVLAPGQHEGVGACRKADDQGISSWGWNLNGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHKTAKDNPPVTFSIAACETQNVSVSVLPCFGLSDRSSVTAKGMWTKMVKDGQFDRENFSSGPSMPSSPGETLCAAVAASAWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTKFYGTSERAAVHLAHDALTHYTRWEEEIAKWQDPILKDEKLPEWYKFTLFNELYFLVAGGTIWIDSTLLSSNKRNNSQDQLEESENAVVRITEAKVDCRKREVVECTTDNSYDSTAHRGHNHLDEKHNRDISRENGTVNTLGKGNSANTPHHSTMKNLQHDDDNDDGGRFLYLEGVEYVMWCTYDVHFYASFALLMLFPRIELNIQRDFAQAVLCEDGRKVKFLAEGNWGTRKVYGAVPHDLGTHDPWHEMNAYNIHDTSKWKDLNPKFVLQVYRDFSATGDLQFGVDVWPAVRAAMEYMEQFDRDADGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMALQLGDRDFAETCKRKFLKAKPVYEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPSLFDDFKIKSSLRKVFDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREIWAGVTYGVAATMILAGMEEEAFTTAEGIFLAGWSEEGSGYWFQTPEAFTIDGHYRSLIYMRPLSIWGMQYALTMPKAVLEAPKINFMDRIHLSPVSGGLHKETGVKKIATKTKCFSSSVFNCAC

Target ORF information:

RefSeq Version	XM_003590653.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003590653.3

ORF Insert Sequence:

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTGTGAAAA AGATTGCAAC GAAAACAAAA 
TGCTTTAGCA GTTCTGTGTT TAATTGTGCT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11423130 ( XM_003590653.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003590701.2 Medicago truncatula Non-lysosomal glucosylceramidase mRNA.

Price & Availability↑

CloneID	OMe207899
Clone ID Related Accession (Same CDS sequence)	XM_003590653.3 , XM_003590653.2
Accession Version	XM_003590653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	Non-lysosomal glucosylceramidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). On Aug 25, 2015 this sequence version replaced XM_003590653.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTGTGAAAA AGATTGCAAC GAAAACAAAA 
TGCTTTAGCA GTTCTGTGTT TAATTGTGCT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003590701.2
CDS	406..3381
Translation	MRDFERMVSGNLFHSRKNSWPTEEYINKTTLQLFDIDNAAPPEQAWRRRLNSHANLLKEFRVTFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFVSREGGNKSFASVLAPGQHEGVGACRKADDQGISSWGWNLNGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHKTAKDNPPVTFSIAACETQNVSVSVLPCFGLSDRSSVTAKGMWTKMVKDGQFDRENFSSGPSMPSSPGETLCAAVAASAWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTKFYGTSERAAVHLAHDALTHYTRWEEEIAKWQDPILKDEKLPEWYKFTLFNELYFLVAGGTIWIDSTLLSSNKRNNSQDQLEESENAVVRITEAKVDCRKREVVECTTDNSYDSTAHRGHNHLDEKHNRDISRENGTVNTLGKGNSANTPHHSTMKNLQHDDDNDDGGRFLYLEGVEYVMWCTYDVHFYASFALLMLFPRIELNIQRDFAQAVLCEDGRKVKFLAEGNWGTRKVYGAVPHDLGTHDPWHEMNAYNIHDTSKWKDLNPKFVLQVYRDFSATGDLQFGVDVWPAVRAAMEYMEQFDRDADGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMALQLGDRDFAETCKRKFLKAKPVYEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPSLFDDFKIKSSLRKVFDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREIWAGVTYGVAATMILAGMEEEAFTTAEGIFLAGWSEEGSGYWFQTPEAFTIDGHYRSLIYMRPLSIWGMQYALTMPKAVLEAPKINFMDRIHLSPVSGGLHKETGVKKIATKTKCFSSSVFNCAC

Target ORF information:

RefSeq Version	XM_003590653.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula Non-lysosomal glucosylceramidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003590653.2

ORF Insert Sequence:

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTGTGAAAA AGATTGCAAC GAAAACAAAA 
TGCTTTAGCA GTTCTGTGTT TAATTGTGCT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11423130 ( XM_013613154.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468608.1 Medicago truncatula Non-lysosomal glucosylceramidase mRNA.

Price & Availability↑

CloneID	OMe211933
Clone ID Related Accession (Same CDS sequence)	XM_013613154.1 , XM_013613154.2
Accession Version	XM_013613154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	Non-lysosomal glucosylceramidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTAACCTCT TCACACTGTT ACTAACCCAA 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468608.1
CDS	406..3354
Translation	MRDFERMVSGNLFHSRKNSWPTEEYINKTTLQLFDIDNAAPPEQAWRRRLNSHANLLKEFRVTFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFVSREGGNKSFASVLAPGQHEGVGACRKADDQGISSWGWNLNGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHKTAKDNPPVTFSIAACETQNVSVSVLPCFGLSDRSSVTAKGMWTKMVKDGQFDRENFSSGPSMPSSPGETLCAAVAASAWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTKFYGTSERAAVHLAHDALTHYTRWEEEIAKWQDPILKDEKLPEWYKFTLFNELYFLVAGGTIWIDSTLLSSNKRNNSQDQLEESENAVVRITEAKVDCRKREVVECTTDNSYDSTAHRGHNHLDEKHNRDISRENGTVNTLGKGNSANTPHHSTMKNLQHDDDNDDGGRFLYLEGVEYVMWCTYDVHFYASFALLMLFPRIELNIQRDFAQAVLCEDGRKVKFLAEGNWGTRKVYGAVPHDLGTHDPWHEMNAYNIHDTSKWKDLNPKFVLQVYRDFSATGDLQFGVDVWPAVRAAMEYMEQFDRDADGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMALQLGDRDFAETCKRKFLKAKPVYEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPSLFDDFKIKSSLRKVFDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREIWAGVTYGVAATMILAGMEEEAFTTAEGIFLAGWSEEGSGYWFQTPEAFTIDGHYRSLIYMRPLSIWGMQYALTMPKAVLEAPKINFMDRIHLSPVSGGLHKETGNLFTLLLTQHS

Target ORF information:

RefSeq Version	XM_013613154.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula Non-lysosomal glucosylceramidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613154.1

ORF Insert Sequence:

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTAACCTCT TCACACTGTT ACTAACCCAA 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11423130 ( XM_013613154.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013468608.1 Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe211933
Clone ID Related Accession (Same CDS sequence)	XM_013613154.1 , XM_013613154.2
Accession Version	XM_013613154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	non-lysosomal glucosylceramidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013613154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTAACCTCT TCACACTGTT ACTAACCCAA 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013468608.1
CDS	400..3348
Translation	MRDFERMVSGNLFHSRKNSWPTEEYINKTTLQLFDIDNAAPPEQAWRRRLNSHANLLKEFRVTFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFVSREGGNKSFASVLAPGQHEGVGACRKADDQGISSWGWNLNGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHKTAKDNPPVTFSIAACETQNVSVSVLPCFGLSDRSSVTAKGMWTKMVKDGQFDRENFSSGPSMPSSPGETLCAAVAASAWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTKFYGTSERAAVHLAHDALTHYTRWEEEIAKWQDPILKDEKLPEWYKFTLFNELYFLVAGGTIWIDSTLLSSNKRNNSQDQLEESENAVVRITEAKVDCRKREVVECTTDNSYDSTAHRGHNHLDEKHNRDISRENGTVNTLGKGNSANTPHHSTMKNLQHDDDNDDGGRFLYLEGVEYVMWCTYDVHFYASFALLMLFPRIELNIQRDFAQAVLCEDGRKVKFLAEGNWGTRKVYGAVPHDLGTHDPWHEMNAYNIHDTSKWKDLNPKFVLQVYRDFSATGDLQFGVDVWPAVRAAMEYMEQFDRDADGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMALQLGDRDFAETCKRKFLKAKPVYEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPSLFDDFKIKSSLRKVFDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREIWAGVTYGVAATMILAGMEEEAFTTAEGIFLAGWSEEGSGYWFQTPEAFTIDGHYRSLIYMRPLSIWGMQYALTMPKAVLEAPKINFMDRIHLSPVSGGLHKETGNLFTLLLTQHS

Target ORF information:

RefSeq Version	XM_013613154.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula non-lysosomal glucosylceramidase (LOC11423130), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013613154.2

ORF Insert Sequence:

ATGAGAGATT TTGAAAGAAT GGTTAGCGGC AACTTATTTC ATTCTAGAAA GAATTCGTGG 
CCTACTGAAG AGTATATCAA TAAAACCACT TTACAATTGT TTGATATTGA TAATGCTGCT 
CCTCCTGAGC AAGCTTGGAG AAGAAGATTA AACAGCCACG CCAATCTTCT TAAAGAATTT 
AGAGTAACTT TTATGGAAGC TATAAAGATG GTTCGATTAG GTATACGCAT GTGGTCATAT 
GTTAGGGAAG AGGCTTCTCA TGGAAGGAAA GCACCCATAG ATCCCTTCAC TCGAGAAAGT 
TGCAAGCCAT CTGCATCTCA AGGGGTTCCA CTTGGAGGGA TGGGGAGTGG CAGCATATCA 
AGAGGATTTA GAGGAGAATT CAGACAATGG CAAATCATTC CTGGTCTATG TGAACCATCA 
CCTGTCATGG CCAACCAGTT CTCTATTTTT GTAAGTAGAG AGGGAGGAAA CAAAAGTTTT 
GCATCAGTTT TGGCTCCTGG TCAGCATGAA GGTGTAGGAG CTTGCAGGAA AGCTGATGAT 
CAGGGTATAT CATCATGGGG ATGGAATCTA AATGGCCAGC ACTCAACTTA CCATGCTTTG 
TTTCCAAGAG CTTGGACTGT TTATGATGGT GAGCCAGACC CAGAACTAAA AATATCATGT 
CGGCAGATAT CCCCTTTCAT ACCACATAAT TACAGAGAAA GCAGTCTACC TGCAGCCGTT 
TTTGTCTATA CGTTGGTAAA CACTGGTAAA GAAAGAGCTA AAGTCAGCCT TTTATTTACT 
TGGGCGAATT CTATTGGTGG GAACTCTCAC TTGTCAGGAG ATCATGTGAA TGAACCTTTC 
ATAGCTGAAG ATGGTGTCTC CGGCGTACTT CTATATCACA AGACAGCGAA AGACAACCCC 
CCTGTTACTT TTTCAATTGC TGCATGTGAA ACACAGAATG TTAGTGTTTC TGTTTTGCCA 
TGTTTTGGGC TGTCCGACCG AAGTAGCGTA ACAGCAAAAG GAATGTGGAC TAAAATGGTG 
AAGGATGGGC AATTTGATCG GGAAAATTTC TCTTCTGGAC CCAGCATGCC CTCATCACCT 
GGAGAGACAC TATGTGCTGC TGTTGCAGCT TCTGCATGGG TGGAACCCCA TGGAAAATGT 
ACCGTTGCAT TTAGTCTTGC ATGGTCATCT CCGAAAGTGA AGTTTGTGAA AGGCAGCACT 
TTCAACAGGA GATATACAAA ATTTTATGGG ACTTCTGAGA GAGCTGCAGT GCACCTGGCT 
CATGATGCAT TGACACATTA TACACGGTGG GAAGAAGAGA TTGCGAAATG GCAGGATCCT 
ATTCTTAAGG ATGAGAAGCT ACCAGAATGG TACAAGTTTA CATTATTTAA CGAACTCTAC 
TTTCTTGTTG CTGGAGGAAC AATTTGGATA GACTCTACTT TGCTATCTTC AAATAAGAGA 
AATAATAGCC AGGATCAGCT AGAAGAGTCG GAAAATGCAG TAGTTCGAAT AACTGAAGCT 
AAAGTGGACT GCAGAAAAAG GGAAGTTGTC GAGTGTACGA CAGATAATTC CTATGATTCT 
ACTGCTCATA GGGGACATAA TCACTTGGAT GAAAAACATA ATAGGGATAT ATCCCGTGAA 
AATGGAACCG TGAATACTCT CGGAAAAGGA AACTCTGCTA ACACTCCACA TCATTCAACA 
ATGAAGAATC TACAACATGA TGATGATAAT GATGATGGTG GAAGGTTTTT GTACTTGGAA 
GGTGTGGAAT ATGTCATGTG GTGCACATAT GATGTGCACT TCTATGCTTC ATTTGCACTT 
CTTATGCTCT TTCCTCGTAT CGAATTAAAT ATACAACGCG ATTTTGCTCA AGCTGTCCTG 
TGCGAAGATG GAAGGAAAGT AAAGTTTCTG GCAGAGGGAA ACTGGGGAAC TCGTAAGGTT 
TATGGGGCTG TCCCACATGA TTTGGGGACA CATGATCCAT GGCATGAAAT GAATGCTTAT 
AACATTCATG ATACTAGTAA GTGGAAAGAC CTGAACCCAA AATTTGTACT TCAGGTGTAT 
CGAGATTTTT CTGCAACAGG TGATTTGCAA TTTGGTGTCG ATGTGTGGCC TGCTGTCCGA 
GCTGCAATGG AGTACATGGA GCAATTCGAT AGAGATGCTG ATGGTCTTAT TGAGAATGAC 
GGTTTCCCTG ATCAAACATA TGATACATGG ACTGTCCATG GTGTGAGTGC TTACTGTGGT 
GGTCTTTGGC TTGCTGCTCT TCAAGCTGCA GCTGCAATGG CCCTTCAACT AGGTGACAGA 
GATTTTGCGG AAACATGCAA AAGGAAGTTT CTGAAGGCTA AGCCAGTATA TGAACAGAAG 
CTGTGGAATG GTTCATATTT TAACTATGAC AGTGGATCAA GTAGTAACAG TAAATCCATT 
CAAGCCGATC AATTGGCTGG GCAATGGTAT ACAGCATCCT CAGGGCTTCC CTCTCTTTTT 
GATGATTTCA AGATCAAAAG TTCTCTCCGG AAGGTTTTTG ATTTCAATGT GATGAAAGTT 
AAAGGCGGCA GAATGGGTGC TGTAAATGGC ATGCATCCCA ACGGTAAGGT GGATGAAACC 
TGTATGCAGT CTCGAGAGAT ATGGGCAGGT GTGACCTACG GTGTTGCTGC AACAATGATA 
CTTGCAGGAA TGGAAGAAGA GGCCTTCACA ACTGCTGAGG GTATATTTCT AGCAGGCTGG 
TCAGAAGAAG GATCCGGATA CTGGTTTCAG ACTCCAGAGG CATTTACAAT TGACGGGCAC 
TATCGGTCCC TCATATATAT GAGGCCACTA TCAATTTGGG GCATGCAATA TGCATTAACT 
ATGCCAAAGG CAGTACTTGA GGCCCCTAAA ATCAACTTCA TGGACAGAAT CCACCTATCT 
CCTGTTAGTG GAGGATTACA TAAAGAAACA GGTAACCTCT TCACACTGTT ACTAACCCAA 
CACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11423130 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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