LOC11414900 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11414900
Entrez Gene ID	11414900
Full Name	DNA mismatch repair protein PMS2, putative
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003591503.2	XP_003591551.1	DNA mismatch repair protein PMS2, putative
XM_003591503.3	XP_003591551.1	DNA mismatch repair protein PMS1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11414900 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11414900 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe214569	XM_003591503.3 Latest version!	Medicago truncatula DNA mismatch repair protein PMS1 (LOC11414900), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe214569	XM_003591503.2	Medicago truncatula DNA mismatch repair protein PMS2, putative mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11414900 ( XM_003591503.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003591551.1 Medicago truncatula DNA mismatch repair protein PMS2, putative mRNA.

Price & Availability↑

CloneID	OMe214569
Clone ID Related Accession (Same CDS sequence)	XM_003591503.2 , XM_003591503.3
Accession Version	XM_003591503.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	DNA mismatch repair protein PMS2, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). On Aug 25, 2015 this sequence version replaced XM_003591503.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGACGATCG AATCGCAAAT AATCAAACCA ATCGCAAAGG GAATCGTTCA CAGAATCTGT 
TCCGGTCAAG TCATCCTCGA CCTCTCCTCC GCCGTTAAAG AACTCGTCGA AAATAGCTTA 
GACGCCGGCG CCACCAGCAT CGAAATCTCT CTTAAAGACT TCGGTGAAGA ATGGTTTCAA 
GTCATCGATA ACGGTTGCGG CATTTCACCA AACAGTTTCA AGGTTCTTGG ACTTAAGCAT 
CATACTTCAA AACTATCTGA GTTTCATGAT CTTCAGTCTC TGACTACTTT TGGATTTAGA 
GGAGAGGCGT TGAGTTCTCT TTGTGCTTTG GGGAATTTGA CTATTGAAAC GAGAACTGTG 
AATGAACCGG TTGCGACGCA TTTGACATTT AATCATTCTG GTGTTCTTGT GGCGGAAAAG 
AAAATTGCGC GACAAATTGG AACGACTGTG ACGGTTAAGA AGTTGTTCTC GAGTCTTCCT 
GTTCGAAGCA AAGAGTTTAA GCGGAATATA CGTAAGGAAT ATGGAAAGTT GGCTTCTCTC 
TTGAATGCAT ATGCCCTTAT AGCAAAAGGT GTTCGATTTG GCTGCACGAA CACGACTGGC 
AAAAATGTAA AATCTGTGGT ACTGAAAACC CAAGGAAATG ATTCTCTAAA AGATAACATC 
ATAACAGTGC TTGGCATGAA CACTTTCAAC TGCTTGGAGC CCATGTCATT ATGTATATCT 
GAGAGCTGCA AAGTTGACGG TTTTCTTTCC AAGCCTGGAC TGGGTAACGG AAGGAATTTG 
GGAGATAGAC AGTATTTTTT TGTAAACGGT AGACCAGTGG ATATGCCAAA AATCGGAAAA 
CTTGTGAATG AGTTATATAG AAGTGCAAAC TCTAAACAAT ATCCCATTGC AATCATGAAT 
TTTACAGTTC CAACGAAAGC ATATGATGTC AATGTGACTC CTGATAAGAG GAAAATATTT 
TTTTCTGAAG AAACTTCCTT ATTGCAAGCC CTTAGAGAGG GCTTACAACA AATTTATTCT 
CCTGATAATG CCTCTTATGC TGTCAATGAA TTTATGCGGC CTGCCGCAAA AGAAGACTGT 
TTTGAGTTGC GTTCCTCTCA GAAGAAGTCT CCTATCGTAA CGAAACCAGC GTCCCTGAAT 
GTGGCGATTC CTCAGGAAGA GCATTATACT GAGTTCAATA CTGCTAGTAT TTCTCGGGAT 
AAGAATAATT CTGACCGCAA CGGTGGTAGT ATATCTCTTA ATGAGCATAA GGAGAAACAC 
ACTACTGATT CTAATAATGC TTCTGAATCT GATGATGGTG ATCTATTCTC ACATGTTGAA 
GAAGGGTTGA TTCGTGAGAG TGGTGGGGGT TTAATGGGCA AGGAGTTCAC ACTCAGAGCG 
CACAAAACTT TAAAGGGTGA CAAGAGTGGA AGACAGATGG CATCTACTCA TATTGCTTTG 
CGCAATCAAG CTACCCTGGT CTCAAGGACA GTTGAGAGTG GGGGCTCTTC TGATAAATAT 
TCATCTGATT CATCAAGACA TGTTCAGTCA ACCCTTAACA ATTTTGTTGC TGTAAGTAAA 
AGAAAACGTG ATGACATCAT TACAGCCTTA TCTGAAGTGC CTGTCCTTAG AAATCAAGCT 
CCGCAATGTA AATTGAAGAC TGTAAATACT GAAACCAATG ACCTGATAAC AAGATCATAT 
CTTCATTTGG ACCAAATTAA TGAAACTTCT ACGCCAAGTG AGATTGAAAA TTTGCAGCAA 
CGTAATCCTG ATGGCATCAA CCACAGCAGT GTAAATTCAC TGTCTTTTAT AGAAGACTCT 
ACTGACAGAG AACCTAACAT GAAACCACAT CAAGAGAATA AAACACATCT AGCTGATACA 
GCATCAGTTA CACCATCTAG CAACAATCTG ATAGATACTA CAGATGATGT TTTAGATTCT 
CCCAAGTCCT CTGGTCAGAA GATATTCTCC AACATGCAAT TTTCTTTTCA AGACCTTAAG 
AGTAGGAGAG AGAAGAGACT TTCTTTGGTA CAATCCAGCA AATACAGATA TGGAAAAGCT 
AATGGCAAAA GTCACTATAC GGCTGCAACT CTGGAGCTTT CACAGCCAGA CATTGAACAG 
CAGAAAGAAA GGGTTTTGGC AGCAGCAGCC ACCGAACTCG AAAGATTGTT CAAGAAGGAA 
TACTTCAGCA GAATGAAGGT GATTGGGCAA TTCAATCTTG GATTTATCAT TGGTAAATTG 
GATCAAGATT TATTCATTGT GGATCAGCAT GCTGCTGACG AGAAATACAA TTTTGAGTGT 
CTGTCCCAAT CAACCATATT GAACCAACAA CCTCTGCTTA GGCCAATAAG ATTGGAGTTA 
TCCCCTGAGG AAGAAATAGT TGCTTCGATT CACATGGACA TAATCAGGAA GAATGGATTT 
ACTTTGGAAG AGGATCTAAA TGCACCACCT GGATGCCGTT ATAAATTAAA GTCTGTCCCC 
TACAGTAAAA ACACCATGTT TGGAGTCGAA GATGTTAAGG ACCTGATTTC AACTCTATCT 
GATGGTGATG GTCATGGAGA ATGTTCCATT ATTGGTAGTT ATAGACAGGA TTCCTCAGAT 
TCAATCTGCC CTCCAAGAGT TCGTGCAATG TTGGCATCTC GAGCATGTCG ATCATCAATT 
ATGATTGGTG ATGCACTTGG AAGAAATGAA ATGCAGAAGA TACTTGAGCA TTTAGCGGAG 
CTGAAATCCC CGTGGAATTG TCCTCATGGC AGGCCGACCA TGCGGCATTT AGTTGACTTG 
ACGAAAATTC ATAAGAGGTC AGAATTAATA ATGCAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003591551.1
CDS	127..2928
Translation	MTIESQIIKPIAKGIVHRICSGQVILDLSSAVKELVENSLDAGATSIEISLKDFGEEWFQVIDNGCGISPNSFKVLGLKHHTSKLSEFHDLQSLTTFGFRGEALSSLCALGNLTIETRTVNEPVATHLTFNHSGVLVAEKKIARQIGTTVTVKKLFSSLPVRSKEFKRNIRKEYGKLASLLNAYALIAKGVRFGCTNTTGKNVKSVVLKTQGNDSLKDNIITVLGMNTFNCLEPMSLCISESCKVDGFLSKPGLGNGRNLGDRQYFFVNGRPVDMPKIGKLVNELYRSANSKQYPIAIMNFTVPTKAYDVNVTPDKRKIFFSEETSLLQALREGLQQIYSPDNASYAVNEFMRPAAKEDCFELRSSQKKSPIVTKPASLNVAIPQEEHYTEFNTASISRDKNNSDRNGGSISLNEHKEKHTTDSNNASESDDGDLFSHVEEGLIRESGGGLMGKEFTLRAHKTLKGDKSGRQMASTHIALRNQATLVSRTVESGGSSDKYSSDSSRHVQSTLNNFVAVSKRKRDDIITALSEVPVLRNQAPQCKLKTVNTETNDLITRSYLHLDQINETSTPSEIENLQQRNPDGINHSSVNSLSFIEDSTDREPNMKPHQENKTHLADTASVTPSSNNLIDTTDDVLDSPKSSGQKIFSNMQFSFQDLKSRREKRLSLVQSSKYRYGKANGKSHYTAATLELSQPDIEQQKERVLAAAATELERLFKKEYFSRMKVIGQFNLGFIIGKLDQDLFIVDQHAADEKYNFECLSQSTILNQQPLLRPIRLELSPEEEIVASIHMDIIRKNGFTLEEDLNAPPGCRYKLKSVPYSKNTMFGVEDVKDLISTLSDGDGHGECSIIGSYRQDSSDSICPPRVRAMLASRACRSSIMIGDALGRNEMQKILEHLAELKSPWNCPHGRPTMRHLVDLTKIHKRSELIMQE

Target ORF information:

RefSeq Version	XM_003591503.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula DNA mismatch repair protein PMS2, putative mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003591503.2

ORF Insert Sequence:

ATGACGATCG AATCGCAAAT AATCAAACCA ATCGCAAAGG GAATCGTTCA CAGAATCTGT 
TCCGGTCAAG TCATCCTCGA CCTCTCCTCC GCCGTTAAAG AACTCGTCGA AAATAGCTTA 
GACGCCGGCG CCACCAGCAT CGAAATCTCT CTTAAAGACT TCGGTGAAGA ATGGTTTCAA 
GTCATCGATA ACGGTTGCGG CATTTCACCA AACAGTTTCA AGGTTCTTGG ACTTAAGCAT 
CATACTTCAA AACTATCTGA GTTTCATGAT CTTCAGTCTC TGACTACTTT TGGATTTAGA 
GGAGAGGCGT TGAGTTCTCT TTGTGCTTTG GGGAATTTGA CTATTGAAAC GAGAACTGTG 
AATGAACCGG TTGCGACGCA TTTGACATTT AATCATTCTG GTGTTCTTGT GGCGGAAAAG 
AAAATTGCGC GACAAATTGG AACGACTGTG ACGGTTAAGA AGTTGTTCTC GAGTCTTCCT 
GTTCGAAGCA AAGAGTTTAA GCGGAATATA CGTAAGGAAT ATGGAAAGTT GGCTTCTCTC 
TTGAATGCAT ATGCCCTTAT AGCAAAAGGT GTTCGATTTG GCTGCACGAA CACGACTGGC 
AAAAATGTAA AATCTGTGGT ACTGAAAACC CAAGGAAATG ATTCTCTAAA AGATAACATC 
ATAACAGTGC TTGGCATGAA CACTTTCAAC TGCTTGGAGC CCATGTCATT ATGTATATCT 
GAGAGCTGCA AAGTTGACGG TTTTCTTTCC AAGCCTGGAC TGGGTAACGG AAGGAATTTG 
GGAGATAGAC AGTATTTTTT TGTAAACGGT AGACCAGTGG ATATGCCAAA AATCGGAAAA 
CTTGTGAATG AGTTATATAG AAGTGCAAAC TCTAAACAAT ATCCCATTGC AATCATGAAT 
TTTACAGTTC CAACGAAAGC ATATGATGTC AATGTGACTC CTGATAAGAG GAAAATATTT 
TTTTCTGAAG AAACTTCCTT ATTGCAAGCC CTTAGAGAGG GCTTACAACA AATTTATTCT 
CCTGATAATG CCTCTTATGC TGTCAATGAA TTTATGCGGC CTGCCGCAAA AGAAGACTGT 
TTTGAGTTGC GTTCCTCTCA GAAGAAGTCT CCTATCGTAA CGAAACCAGC GTCCCTGAAT 
GTGGCGATTC CTCAGGAAGA GCATTATACT GAGTTCAATA CTGCTAGTAT TTCTCGGGAT 
AAGAATAATT CTGACCGCAA CGGTGGTAGT ATATCTCTTA ATGAGCATAA GGAGAAACAC 
ACTACTGATT CTAATAATGC TTCTGAATCT GATGATGGTG ATCTATTCTC ACATGTTGAA 
GAAGGGTTGA TTCGTGAGAG TGGTGGGGGT TTAATGGGCA AGGAGTTCAC ACTCAGAGCG 
CACAAAACTT TAAAGGGTGA CAAGAGTGGA AGACAGATGG CATCTACTCA TATTGCTTTG 
CGCAATCAAG CTACCCTGGT CTCAAGGACA GTTGAGAGTG GGGGCTCTTC TGATAAATAT 
TCATCTGATT CATCAAGACA TGTTCAGTCA ACCCTTAACA ATTTTGTTGC TGTAAGTAAA 
AGAAAACGTG ATGACATCAT TACAGCCTTA TCTGAAGTGC CTGTCCTTAG AAATCAAGCT 
CCGCAATGTA AATTGAAGAC TGTAAATACT GAAACCAATG ACCTGATAAC AAGATCATAT 
CTTCATTTGG ACCAAATTAA TGAAACTTCT ACGCCAAGTG AGATTGAAAA TTTGCAGCAA 
CGTAATCCTG ATGGCATCAA CCACAGCAGT GTAAATTCAC TGTCTTTTAT AGAAGACTCT 
ACTGACAGAG AACCTAACAT GAAACCACAT CAAGAGAATA AAACACATCT AGCTGATACA 
GCATCAGTTA CACCATCTAG CAACAATCTG ATAGATACTA CAGATGATGT TTTAGATTCT 
CCCAAGTCCT CTGGTCAGAA GATATTCTCC AACATGCAAT TTTCTTTTCA AGACCTTAAG 
AGTAGGAGAG AGAAGAGACT TTCTTTGGTA CAATCCAGCA AATACAGATA TGGAAAAGCT 
AATGGCAAAA GTCACTATAC GGCTGCAACT CTGGAGCTTT CACAGCCAGA CATTGAACAG 
CAGAAAGAAA GGGTTTTGGC AGCAGCAGCC ACCGAACTCG AAAGATTGTT CAAGAAGGAA 
TACTTCAGCA GAATGAAGGT GATTGGGCAA TTCAATCTTG GATTTATCAT TGGTAAATTG 
GATCAAGATT TATTCATTGT GGATCAGCAT GCTGCTGACG AGAAATACAA TTTTGAGTGT 
CTGTCCCAAT CAACCATATT GAACCAACAA CCTCTGCTTA GGCCAATAAG ATTGGAGTTA 
TCCCCTGAGG AAGAAATAGT TGCTTCGATT CACATGGACA TAATCAGGAA GAATGGATTT 
ACTTTGGAAG AGGATCTAAA TGCACCACCT GGATGCCGTT ATAAATTAAA GTCTGTCCCC 
TACAGTAAAA ACACCATGTT TGGAGTCGAA GATGTTAAGG ACCTGATTTC AACTCTATCT 
GATGGTGATG GTCATGGAGA ATGTTCCATT ATTGGTAGTT ATAGACAGGA TTCCTCAGAT 
TCAATCTGCC CTCCAAGAGT TCGTGCAATG TTGGCATCTC GAGCATGTCG ATCATCAATT 
ATGATTGGTG ATGCACTTGG AAGAAATGAA ATGCAGAAGA TACTTGAGCA TTTAGCGGAG 
CTGAAATCCC CGTGGAATTG TCCTCATGGC AGGCCGACCA TGCGGCATTT AGTTGACTTG 
ACGAAAATTC ATAAGAGGTC AGAATTAATA ATGCAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11414900 ( XM_003591503.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003591551.1 Medicago truncatula DNA mismatch repair protein PMS1 (LOC11414900), mRNA.

Price & Availability↑

CloneID	OMe214569
Clone ID Related Accession (Same CDS sequence)	XM_003591503.2 , XM_003591503.3
Accession Version	XM_003591503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	DNA mismatch repair protein PMS1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003591503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATCG AATCGCAAAT AATCAAACCA ATCGCAAAGG GAATCGTTCA CAGAATCTGT 
TCCGGTCAAG TCATCCTCGA CCTCTCCTCC GCCGTTAAAG AACTCGTCGA AAATAGCTTA 
GACGCCGGCG CCACCAGCAT CGAAATCTCT CTTAAAGACT TCGGTGAAGA ATGGTTTCAA 
GTCATCGATA ACGGTTGCGG CATTTCACCA AACAGTTTCA AGGTTCTTGG ACTTAAGCAT 
CATACTTCAA AACTATCTGA GTTTCATGAT CTTCAGTCTC TGACTACTTT TGGATTTAGA 
GGAGAGGCGT TGAGTTCTCT TTGTGCTTTG GGGAATTTGA CTATTGAAAC GAGAACTGTG 
AATGAACCGG TTGCGACGCA TTTGACATTT AATCATTCTG GTGTTCTTGT GGCGGAAAAG 
AAAATTGCGC GACAAATTGG AACGACTGTG ACGGTTAAGA AGTTGTTCTC GAGTCTTCCT 
GTTCGAAGCA AAGAGTTTAA GCGGAATATA CGTAAGGAAT ATGGAAAGTT GGCTTCTCTC 
TTGAATGCAT ATGCCCTTAT AGCAAAAGGT GTTCGATTTG GCTGCACGAA CACGACTGGC 
AAAAATGTAA AATCTGTGGT ACTGAAAACC CAAGGAAATG ATTCTCTAAA AGATAACATC 
ATAACAGTGC TTGGCATGAA CACTTTCAAC TGCTTGGAGC CCATGTCATT ATGTATATCT 
GAGAGCTGCA AAGTTGACGG TTTTCTTTCC AAGCCTGGAC TGGGTAACGG AAGGAATTTG 
GGAGATAGAC AGTATTTTTT TGTAAACGGT AGACCAGTGG ATATGCCAAA AATCGGAAAA 
CTTGTGAATG AGTTATATAG AAGTGCAAAC TCTAAACAAT ATCCCATTGC AATCATGAAT 
TTTACAGTTC CAACGAAAGC ATATGATGTC AATGTGACTC CTGATAAGAG GAAAATATTT 
TTTTCTGAAG AAACTTCCTT ATTGCAAGCC CTTAGAGAGG GCTTACAACA AATTTATTCT 
CCTGATAATG CCTCTTATGC TGTCAATGAA TTTATGCGGC CTGCCGCAAA AGAAGACTGT 
TTTGAGTTGC GTTCCTCTCA GAAGAAGTCT CCTATCGTAA CGAAACCAGC GTCCCTGAAT 
GTGGCGATTC CTCAGGAAGA GCATTATACT GAGTTCAATA CTGCTAGTAT TTCTCGGGAT 
AAGAATAATT CTGACCGCAA CGGTGGTAGT ATATCTCTTA ATGAGCATAA GGAGAAACAC 
ACTACTGATT CTAATAATGC TTCTGAATCT GATGATGGTG ATCTATTCTC ACATGTTGAA 
GAAGGGTTGA TTCGTGAGAG TGGTGGGGGT TTAATGGGCA AGGAGTTCAC ACTCAGAGCG 
CACAAAACTT TAAAGGGTGA CAAGAGTGGA AGACAGATGG CATCTACTCA TATTGCTTTG 
CGCAATCAAG CTACCCTGGT CTCAAGGACA GTTGAGAGTG GGGGCTCTTC TGATAAATAT 
TCATCTGATT CATCAAGACA TGTTCAGTCA ACCCTTAACA ATTTTGTTGC TGTAAGTAAA 
AGAAAACGTG ATGACATCAT TACAGCCTTA TCTGAAGTGC CTGTCCTTAG AAATCAAGCT 
CCGCAATGTA AATTGAAGAC TGTAAATACT GAAACCAATG ACCTGATAAC AAGATCATAT 
CTTCATTTGG ACCAAATTAA TGAAACTTCT ACGCCAAGTG AGATTGAAAA TTTGCAGCAA 
CGTAATCCTG ATGGCATCAA CCACAGCAGT GTAAATTCAC TGTCTTTTAT AGAAGACTCT 
ACTGACAGAG AACCTAACAT GAAACCACAT CAAGAGAATA AAACACATCT AGCTGATACA 
GCATCAGTTA CACCATCTAG CAACAATCTG ATAGATACTA CAGATGATGT TTTAGATTCT 
CCCAAGTCCT CTGGTCAGAA GATATTCTCC AACATGCAAT TTTCTTTTCA AGACCTTAAG 
AGTAGGAGAG AGAAGAGACT TTCTTTGGTA CAATCCAGCA AATACAGATA TGGAAAAGCT 
AATGGCAAAA GTCACTATAC GGCTGCAACT CTGGAGCTTT CACAGCCAGA CATTGAACAG 
CAGAAAGAAA GGGTTTTGGC AGCAGCAGCC ACCGAACTCG AAAGATTGTT CAAGAAGGAA 
TACTTCAGCA GAATGAAGGT GATTGGGCAA TTCAATCTTG GATTTATCAT TGGTAAATTG 
GATCAAGATT TATTCATTGT GGATCAGCAT GCTGCTGACG AGAAATACAA TTTTGAGTGT 
CTGTCCCAAT CAACCATATT GAACCAACAA CCTCTGCTTA GGCCAATAAG ATTGGAGTTA 
TCCCCTGAGG AAGAAATAGT TGCTTCGATT CACATGGACA TAATCAGGAA GAATGGATTT 
ACTTTGGAAG AGGATCTAAA TGCACCACCT GGATGCCGTT ATAAATTAAA GTCTGTCCCC 
TACAGTAAAA ACACCATGTT TGGAGTCGAA GATGTTAAGG ACCTGATTTC AACTCTATCT 
GATGGTGATG GTCATGGAGA ATGTTCCATT ATTGGTAGTT ATAGACAGGA TTCCTCAGAT 
TCAATCTGCC CTCCAAGAGT TCGTGCAATG TTGGCATCTC GAGCATGTCG ATCATCAATT 
ATGATTGGTG ATGCACTTGG AAGAAATGAA ATGCAGAAGA TACTTGAGCA TTTAGCGGAG 
CTGAAATCCC CGTGGAATTG TCCTCATGGC AGGCCGACCA TGCGGCATTT AGTTGACTTG 
ACGAAAATTC ATAAGAGGTC AGAATTAATA ATGCAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003591551.1
CDS	158..2959
Translation	MTIESQIIKPIAKGIVHRICSGQVILDLSSAVKELVENSLDAGATSIEISLKDFGEEWFQVIDNGCGISPNSFKVLGLKHHTSKLSEFHDLQSLTTFGFRGEALSSLCALGNLTIETRTVNEPVATHLTFNHSGVLVAEKKIARQIGTTVTVKKLFSSLPVRSKEFKRNIRKEYGKLASLLNAYALIAKGVRFGCTNTTGKNVKSVVLKTQGNDSLKDNIITVLGMNTFNCLEPMSLCISESCKVDGFLSKPGLGNGRNLGDRQYFFVNGRPVDMPKIGKLVNELYRSANSKQYPIAIMNFTVPTKAYDVNVTPDKRKIFFSEETSLLQALREGLQQIYSPDNASYAVNEFMRPAAKEDCFELRSSQKKSPIVTKPASLNVAIPQEEHYTEFNTASISRDKNNSDRNGGSISLNEHKEKHTTDSNNASESDDGDLFSHVEEGLIRESGGGLMGKEFTLRAHKTLKGDKSGRQMASTHIALRNQATLVSRTVESGGSSDKYSSDSSRHVQSTLNNFVAVSKRKRDDIITALSEVPVLRNQAPQCKLKTVNTETNDLITRSYLHLDQINETSTPSEIENLQQRNPDGINHSSVNSLSFIEDSTDREPNMKPHQENKTHLADTASVTPSSNNLIDTTDDVLDSPKSSGQKIFSNMQFSFQDLKSRREKRLSLVQSSKYRYGKANGKSHYTAATLELSQPDIEQQKERVLAAAATELERLFKKEYFSRMKVIGQFNLGFIIGKLDQDLFIVDQHAADEKYNFECLSQSTILNQQPLLRPIRLELSPEEEIVASIHMDIIRKNGFTLEEDLNAPPGCRYKLKSVPYSKNTMFGVEDVKDLISTLSDGDGHGECSIIGSYRQDSSDSICPPRVRAMLASRACRSSIMIGDALGRNEMQKILEHLAELKSPWNCPHGRPTMRHLVDLTKIHKRSELIMQE

Target ORF information:

RefSeq Version	XM_003591503.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula DNA mismatch repair protein PMS1 (LOC11414900), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003591503.3

ORF Insert Sequence:

ATGACGATCG AATCGCAAAT AATCAAACCA ATCGCAAAGG GAATCGTTCA CAGAATCTGT 
TCCGGTCAAG TCATCCTCGA CCTCTCCTCC GCCGTTAAAG AACTCGTCGA AAATAGCTTA 
GACGCCGGCG CCACCAGCAT CGAAATCTCT CTTAAAGACT TCGGTGAAGA ATGGTTTCAA 
GTCATCGATA ACGGTTGCGG CATTTCACCA AACAGTTTCA AGGTTCTTGG ACTTAAGCAT 
CATACTTCAA AACTATCTGA GTTTCATGAT CTTCAGTCTC TGACTACTTT TGGATTTAGA 
GGAGAGGCGT TGAGTTCTCT TTGTGCTTTG GGGAATTTGA CTATTGAAAC GAGAACTGTG 
AATGAACCGG TTGCGACGCA TTTGACATTT AATCATTCTG GTGTTCTTGT GGCGGAAAAG 
AAAATTGCGC GACAAATTGG AACGACTGTG ACGGTTAAGA AGTTGTTCTC GAGTCTTCCT 
GTTCGAAGCA AAGAGTTTAA GCGGAATATA CGTAAGGAAT ATGGAAAGTT GGCTTCTCTC 
TTGAATGCAT ATGCCCTTAT AGCAAAAGGT GTTCGATTTG GCTGCACGAA CACGACTGGC 
AAAAATGTAA AATCTGTGGT ACTGAAAACC CAAGGAAATG ATTCTCTAAA AGATAACATC 
ATAACAGTGC TTGGCATGAA CACTTTCAAC TGCTTGGAGC CCATGTCATT ATGTATATCT 
GAGAGCTGCA AAGTTGACGG TTTTCTTTCC AAGCCTGGAC TGGGTAACGG AAGGAATTTG 
GGAGATAGAC AGTATTTTTT TGTAAACGGT AGACCAGTGG ATATGCCAAA AATCGGAAAA 
CTTGTGAATG AGTTATATAG AAGTGCAAAC TCTAAACAAT ATCCCATTGC AATCATGAAT 
TTTACAGTTC CAACGAAAGC ATATGATGTC AATGTGACTC CTGATAAGAG GAAAATATTT 
TTTTCTGAAG AAACTTCCTT ATTGCAAGCC CTTAGAGAGG GCTTACAACA AATTTATTCT 
CCTGATAATG CCTCTTATGC TGTCAATGAA TTTATGCGGC CTGCCGCAAA AGAAGACTGT 
TTTGAGTTGC GTTCCTCTCA GAAGAAGTCT CCTATCGTAA CGAAACCAGC GTCCCTGAAT 
GTGGCGATTC CTCAGGAAGA GCATTATACT GAGTTCAATA CTGCTAGTAT TTCTCGGGAT 
AAGAATAATT CTGACCGCAA CGGTGGTAGT ATATCTCTTA ATGAGCATAA GGAGAAACAC 
ACTACTGATT CTAATAATGC TTCTGAATCT GATGATGGTG ATCTATTCTC ACATGTTGAA 
GAAGGGTTGA TTCGTGAGAG TGGTGGGGGT TTAATGGGCA AGGAGTTCAC ACTCAGAGCG 
CACAAAACTT TAAAGGGTGA CAAGAGTGGA AGACAGATGG CATCTACTCA TATTGCTTTG 
CGCAATCAAG CTACCCTGGT CTCAAGGACA GTTGAGAGTG GGGGCTCTTC TGATAAATAT 
TCATCTGATT CATCAAGACA TGTTCAGTCA ACCCTTAACA ATTTTGTTGC TGTAAGTAAA 
AGAAAACGTG ATGACATCAT TACAGCCTTA TCTGAAGTGC CTGTCCTTAG AAATCAAGCT 
CCGCAATGTA AATTGAAGAC TGTAAATACT GAAACCAATG ACCTGATAAC AAGATCATAT 
CTTCATTTGG ACCAAATTAA TGAAACTTCT ACGCCAAGTG AGATTGAAAA TTTGCAGCAA 
CGTAATCCTG ATGGCATCAA CCACAGCAGT GTAAATTCAC TGTCTTTTAT AGAAGACTCT 
ACTGACAGAG AACCTAACAT GAAACCACAT CAAGAGAATA AAACACATCT AGCTGATACA 
GCATCAGTTA CACCATCTAG CAACAATCTG ATAGATACTA CAGATGATGT TTTAGATTCT 
CCCAAGTCCT CTGGTCAGAA GATATTCTCC AACATGCAAT TTTCTTTTCA AGACCTTAAG 
AGTAGGAGAG AGAAGAGACT TTCTTTGGTA CAATCCAGCA AATACAGATA TGGAAAAGCT 
AATGGCAAAA GTCACTATAC GGCTGCAACT CTGGAGCTTT CACAGCCAGA CATTGAACAG 
CAGAAAGAAA GGGTTTTGGC AGCAGCAGCC ACCGAACTCG AAAGATTGTT CAAGAAGGAA 
TACTTCAGCA GAATGAAGGT GATTGGGCAA TTCAATCTTG GATTTATCAT TGGTAAATTG 
GATCAAGATT TATTCATTGT GGATCAGCAT GCTGCTGACG AGAAATACAA TTTTGAGTGT 
CTGTCCCAAT CAACCATATT GAACCAACAA CCTCTGCTTA GGCCAATAAG ATTGGAGTTA 
TCCCCTGAGG AAGAAATAGT TGCTTCGATT CACATGGACA TAATCAGGAA GAATGGATTT 
ACTTTGGAAG AGGATCTAAA TGCACCACCT GGATGCCGTT ATAAATTAAA GTCTGTCCCC 
TACAGTAAAA ACACCATGTT TGGAGTCGAA GATGTTAAGG ACCTGATTTC AACTCTATCT 
GATGGTGATG GTCATGGAGA ATGTTCCATT ATTGGTAGTT ATAGACAGGA TTCCTCAGAT 
TCAATCTGCC CTCCAAGAGT TCGTGCAATG TTGGCATCTC GAGCATGTCG ATCATCAATT 
ATGATTGGTG ATGCACTTGG AAGAAATGAA ATGCAGAAGA TACTTGAGCA TTTAGCGGAG 
CTGAAATCCC CGTGGAATTG TCCTCATGGC AGGCCGACCA TGCGGCATTT AGTTGACTTG 
ACGAAAATTC ATAAGAGGTC AGAATTAATA ATGCAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11414900 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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