LOC11414513 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11414513
Entrez Gene ID	11414513
Full Name	MAP kinase kinase kinase-like protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003588959.2	XP_003589007.2	MAP kinase kinase kinase-like protein
XM_013610441.1	XP_013465895.1	MAP kinase kinase kinase-like protein
XM_003588959.3	XP_003589007.2	probable serine/threonine-protein kinase SIS8

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11414513 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11414513 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe207639	XM_003588959.3 Latest version!	Medicago truncatula probable serine/threonine-protein kinase SIS8 (LOC11414513), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe208824	XM_013610441.1 Latest version!	Medicago truncatula MAP kinase kinase kinase-like protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMe207639	XM_003588959.2	Medicago truncatula MAP kinase kinase kinase-like protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11414513 ( XM_003588959.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003589007.2 Medicago truncatula MAP kinase kinase kinase-like protein mRNA.

Price & Availability↑

CloneID	OMe207639
Clone ID Related Accession (Same CDS sequence)	XM_003588959.2 , XM_003588959.3
Accession Version	XM_003588959.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	MAP kinase kinase kinase-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). On Aug 25, 2015 this sequence version replaced XM_003588959.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTCTGTG ATTTTGGTCT GTCACGCATG AAGCACCATA CATATTTGTC ATCGAAGTCA 
TGTGCTGGAA CGCCTGAGTG GATGGCGCCA GAAGTTTTAC GCAACGAGCC TGCCAATGAG 
AAGTGTGATG TATATAGTTT TGGTGTGATC TTGTGGGAGT TAACTACCAC GAAAATCCCA 
TGGCATGGCA TGAACCCAAT GCAGGTTGTT GGAGCTGTTG GATTTCAAAA TAAACGTCTT 
GAGATTCCAG AAGAAATGGA TCCCGGAGTA GCTCAAATAA TACGTGATTG CTGGCAAACG 
GAGCCACACT TGCGGCCATC CTTCTCACAG CTGATGTCTC GTCTTTACCG TCTTCGCCAA 
TTGGTTGCCC GTAAAACGGG CTCTACACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003589007.2
CDS	188..2980
Translation	MPKMKQLLRKLHIGGGAPTSPINNHNTIANANANANVALPLSHSDSLTTTPSPSPSPSPSPTVVQNPRNDGAGVNDFNLLQEEEFQVQLALAISASDSDPKDVDESAQIDAAKQISLGYSASLTDTPALVQFQSLRYWNYNVIAYDEKVMDGFYDVYGIDASLIERGKMPLLVDLKTVPTSRNVDYEVISVNRVVDVELSQLEEKARALFEECSVTELGLFLSGLIQKLADVVVNRMGGPVGSADNIMTKWAMRSRELRDSLRTVVLPLGCLDVGLSRHRALLFKVLADRINIPCMLVKGSYYTGTDDGAVNLIKADDGSEYIIDMMGAPGTLIPAEVPSSQIQNYGFAGRDFAEIAGQHNNLYPMLGDETDVLGVLSDCTVGRVQTKELIKIGSQTKPDEINHAKVNEAGRFKHTDAYGSSSHPESSHAENMHVKNVSQYVLSAAKNPEFASKLHTILLESGASPPSDLFSDMNSRHGGLDTVQADQNRLLLSYDKSLLLPQGVGSAGNTRLCQAVAEQQKELHTDAIEFYDSLQSSCTRNAFATVSGKDSDIEQSNPLIVDFACLNTHKTCKEKCPESSLPKTVLSCKRHNGVECFCDDDESGPRNEAGASSNNIELGNDSVIQINETVIGDCVLYDDDKTKKVHPILGEDTQWEIQWEDLVVGERIGIGSYGEVYRADCNGTEVAVKKFLDQDVSGDALDQFKSEIEIMLRLRHPNVVLFMGAITRPPHFSILTEFLPRGSLYRILHRPNLVLDEKRRLRMALDVAKGMNYLHTSHPPVVHRDLKTPNLLVDRNWVVKVCDFGLSRMKHHTYLSSKSCAGTPEWMAPEVLRNEPANEKCDVYSFGVILWELTTTKIPWHGMNPMQVVGAVGFQNKRLEIPEEMDPGVAQIIRDCWQTEPHLRPSFSQLMSRLYRLRQLVARKTGSTH

Target ORF information:

RefSeq Version	XM_003588959.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula MAP kinase kinase kinase-like protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588959.2

ORF Insert Sequence:

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTCTGTG ATTTTGGTCT GTCACGCATG AAGCACCATA CATATTTGTC ATCGAAGTCA 
TGTGCTGGAA CGCCTGAGTG GATGGCGCCA GAAGTTTTAC GCAACGAGCC TGCCAATGAG 
AAGTGTGATG TATATAGTTT TGGTGTGATC TTGTGGGAGT TAACTACCAC GAAAATCCCA 
TGGCATGGCA TGAACCCAAT GCAGGTTGTT GGAGCTGTTG GATTTCAAAA TAAACGTCTT 
GAGATTCCAG AAGAAATGGA TCCCGGAGTA GCTCAAATAA TACGTGATTG CTGGCAAACG 
GAGCCACACT TGCGGCCATC CTTCTCACAG CTGATGTCTC GTCTTTACCG TCTTCGCCAA 
TTGGTTGCCC GTAAAACGGG CTCTACACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11414513 ( XM_003588959.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003589007.2 Medicago truncatula probable serine/threonine-protein kinase SIS8 (LOC11414513), mRNA.

Price & Availability↑

CloneID	OMe207639
Clone ID Related Accession (Same CDS sequence)	XM_003588959.2 , XM_003588959.3
Accession Version	XM_003588959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	probable serine/threonine-protein kinase SIS8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016407.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003588959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTCTGTG ATTTTGGTCT GTCACGCATG AAGCACCATA CATATTTGTC ATCGAAGTCA 
TGTGCTGGAA CGCCTGAGTG GATGGCGCCA GAAGTTTTAC GCAACGAGCC TGCCAATGAG 
AAGTGTGATG TATATAGTTT TGGTGTGATC TTGTGGGAGT TAACTACCAC GAAAATCCCA 
TGGCATGGCA TGAACCCAAT GCAGGTTGTT GGAGCTGTTG GATTTCAAAA TAAACGTCTT 
GAGATTCCAG AAGAAATGGA TCCCGGAGTA GCTCAAATAA TACGTGATTG CTGGCAAACG 
GAGCCACACT TGCGGCCATC CTTCTCACAG CTGATGTCTC GTCTTTACCG TCTTCGCCAA 
TTGGTTGCCC GTAAAACGGG CTCTACACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003589007.2
CDS	205..2997
Translation	MPKMKQLLRKLHIGGGAPTSPINNHNTIANANANANVALPLSHSDSLTTTPSPSPSPSPSPTVVQNPRNDGAGVNDFNLLQEEEFQVQLALAISASDSDPKDVDESAQIDAAKQISLGYSASLTDTPALVQFQSLRYWNYNVIAYDEKVMDGFYDVYGIDASLIERGKMPLLVDLKTVPTSRNVDYEVISVNRVVDVELSQLEEKARALFEECSVTELGLFLSGLIQKLADVVVNRMGGPVGSADNIMTKWAMRSRELRDSLRTVVLPLGCLDVGLSRHRALLFKVLADRINIPCMLVKGSYYTGTDDGAVNLIKADDGSEYIIDMMGAPGTLIPAEVPSSQIQNYGFAGRDFAEIAGQHNNLYPMLGDETDVLGVLSDCTVGRVQTKELIKIGSQTKPDEINHAKVNEAGRFKHTDAYGSSSHPESSHAENMHVKNVSQYVLSAAKNPEFASKLHTILLESGASPPSDLFSDMNSRHGGLDTVQADQNRLLLSYDKSLLLPQGVGSAGNTRLCQAVAEQQKELHTDAIEFYDSLQSSCTRNAFATVSGKDSDIEQSNPLIVDFACLNTHKTCKEKCPESSLPKTVLSCKRHNGVECFCDDDESGPRNEAGASSNNIELGNDSVIQINETVIGDCVLYDDDKTKKVHPILGEDTQWEIQWEDLVVGERIGIGSYGEVYRADCNGTEVAVKKFLDQDVSGDALDQFKSEIEIMLRLRHPNVVLFMGAITRPPHFSILTEFLPRGSLYRILHRPNLVLDEKRRLRMALDVAKGMNYLHTSHPPVVHRDLKTPNLLVDRNWVVKVCDFGLSRMKHHTYLSSKSCAGTPEWMAPEVLRNEPANEKCDVYSFGVILWELTTTKIPWHGMNPMQVVGAVGFQNKRLEIPEEMDPGVAQIIRDCWQTEPHLRPSFSQLMSRLYRLRQLVARKTGSTH

Target ORF information:

RefSeq Version	XM_003588959.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula probable serine/threonine-protein kinase SIS8 (LOC11414513), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588959.3

ORF Insert Sequence:

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTCTGTG ATTTTGGTCT GTCACGCATG AAGCACCATA CATATTTGTC ATCGAAGTCA 
TGTGCTGGAA CGCCTGAGTG GATGGCGCCA GAAGTTTTAC GCAACGAGCC TGCCAATGAG 
AAGTGTGATG TATATAGTTT TGGTGTGATC TTGTGGGAGT TAACTACCAC GAAAATCCCA 
TGGCATGGCA TGAACCCAAT GCAGGTTGTT GGAGCTGTTG GATTTCAAAA TAAACGTCTT 
GAGATTCCAG AAGAAATGGA TCCCGGAGTA GCTCAAATAA TACGTGATTG CTGGCAAACG 
GAGCCACACT TGCGGCCATC CTTCTCACAG CTGATGTCTC GTCTTTACCG TCTTCGCCAA 
TTGGTTGCCC GTAAAACGGG CTCTACACAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11414513 ( XM_013610441.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013465895.1 Medicago truncatula MAP kinase kinase kinase-like protein mRNA.

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CloneID	OMe208824
Clone ID Related Accession (Same CDS sequence)	XM_013610441.1
Accession Version	XM_013610441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	MAP kinase kinase kinase-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016407). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTAATAG TCATCATACT TAATTTCTTT GTCTTCGGAA TTTACCTCAC AATAGCTGAA 
TATAAAAGTA ACATCAAATC AATTGAGTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013465895.1
CDS	188..2680
Translation	MPKMKQLLRKLHIGGGAPTSPINNHNTIANANANANVALPLSHSDSLTTTPSPSPSPSPSPTVVQNPRNDGAGVNDFNLLQEEEFQVQLALAISASDSDPKDVDESAQIDAAKQISLGYSASLTDTPALVQFQSLRYWNYNVIAYDEKVMDGFYDVYGIDASLIERGKMPLLVDLKTVPTSRNVDYEVISVNRVVDVELSQLEEKARALFEECSVTELGLFLSGLIQKLADVVVNRMGGPVGSADNIMTKWAMRSRELRDSLRTVVLPLGCLDVGLSRHRALLFKVLADRINIPCMLVKGSYYTGTDDGAVNLIKADDGSEYIIDMMGAPGTLIPAEVPSSQIQNYGFAGRDFAEIAGQHNNLYPMLGDETDVLGVLSDCTVGRVQTKELIKIGSQTKPDEINHAKVNEAGRFKHTDAYGSSSHPESSHAENMHVKNVSQYVLSAAKNPEFASKLHTILLESGASPPSDLFSDMNSRHGGLDTVQADQNRLLLSYDKSLLLPQGVGSAGNTRLCQAVAEQQKELHTDAIEFYDSLQSSCTRNAFATVSGKDSDIEQSNPLIVDFACLNTHKTCKEKCPESSLPKTVLSCKRHNGVECFCDDDESGPRNEAGASSNNIELGNDSVIQINETVIGDCVLYDDDKTKKVHPILGEDTQWEIQWEDLVVGERIGIGSYGEVYRADCNGTEVAVKKFLDQDVSGDALDQFKSEIEIMLRLRHPNVVLFMGAITRPPHFSILTEFLPRGSLYRILHRPNLVLDEKRRLRMALDVAKGMNYLHTSHPPVVHRDLKTPNLLVDRNWVVKVIVIILNFFVFGIYLTIAEYKSNIKSIEC

Target ORF information:

RefSeq Version	XM_013610441.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula MAP kinase kinase kinase-like protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013610441.1

ORF Insert Sequence:

ATGCCTAAAA TGAAGCAACT CCTTCGAAAG CTTCATATCG GTGGTGGCGC TCCTACTTCT 
CCAATCAACA ATCACAATAC AATCGCAAAC GCAAACGCAA ACGCAAACGT AGCATTGCCA 
CTTTCTCACT CCGATTCTCT CACTACAACT CCTTCTCCTT CGCCATCTCC TTCTCCGTCT 
CCGACGGTTG TTCAGAATCC GCGAAACGAC GGCGCCGGTG TGAACGACTT TAATCTGTTA 
CAGGAAGAAG AGTTTCAGGT TCAATTAGCG CTTGCGATTA GTGCTTCTGA TTCCGATCCG 
AAGGATGTTG ATGAATCTGC TCAGATCGAT GCTGCTAAAC AGATCAGCCT TGGCTATTCT 
GCTTCTCTTA CGGATACTCC TGCACTTGTT CAGTTTCAAT CGCTTCGTTA TTGGAATTAT 
AATGTCATTG CTTATGATGA GAAAGTGATG GATGGGTTTT ATGATGTCTA TGGGATCGAT 
GCAAGTTTAA TTGAACGTGG AAAGATGCCA TTGTTGGTGG ATCTGAAAAC TGTGCCCACC 
TCACGAAATG TTGATTATGA AGTAATCTCG GTGAACCGTG TTGTTGATGT CGAGCTAAGT 
CAGCTTGAGG AAAAAGCCCG TGCCTTGTTT GAAGAGTGTT CTGTTACCGA ACTAGGACTG 
TTTTTGAGTG GCTTAATCCA GAAACTTGCT GATGTTGTTG TTAATAGAAT GGGTGGACCA 
GTTGGTAGTG CTGATAATAT CATGACAAAG TGGGCTATGA GGAGCCGTGA ATTGCGAGAT 
TCTTTAAGAA CTGTCGTCCT TCCTCTTGGC TGTCTAGATG TTGGACTTTC ACGTCACCGA 
GCCCTACTTT TTAAGGTACT TGCTGATAGG ATTAATATTC CCTGCATGCT TGTAAAAGGG 
AGCTATTACA CAGGAACCGA TGATGGGGCT GTGAATTTGA TTAAAGCTGA CGATGGAAGT 
GAATACATTA TTGATATGAT GGGTGCTCCT GGTACTCTTA TTCCTGCTGA GGTGCCCAGC 
AGTCAGATCC AAAACTATGG TTTTGCTGGT AGGGATTTTG CAGAGATTGC AGGACAACAT 
AACAACTTAT ATCCCATGCT TGGTGATGAA ACTGATGTGC TGGGGGTATT GTCTGATTGT 
ACCGTAGGGA GGGTCCAGAC AAAAGAATTA ATAAAAATTG GAAGTCAAAC AAAACCAGAT 
GAAATAAATC ATGCTAAGGT AAATGAAGCA GGGAGATTTA AGCATACAGA TGCGTATGGA 
TCCTCATCAC ATCCAGAGTC ATCACATGCA GAAAACATGC ATGTAAAAAA TGTGTCACAG 
TATGTTCTCA GTGCCGCAAA GAACCCAGAG TTTGCCTCAA AACTGCACAC TATTCTTTTA 
GAAAGTGGTG CATCACCTCC TTCTGATCTT TTTTCAGATA TGAATTCCCG ACATGGGGGT 
TTAGATACTG TTCAAGCTGA CCAAAATAGG CTGTTATTAA GTTATGACAA GTCTCTCTTA 
CTCCCTCAAG GAGTGGGCTC TGCTGGTAAT ACTAGGTTAT GTCAAGCAGT GGCCGAACAG 
CAAAAAGAAT TACACACTGA TGCCATTGAA TTTTATGATT CTTTGCAAAG TAGTTGTACA 
AGGAATGCTT TTGCAACTGT TTCCGGTAAA GATAGCGATA TTGAGCAGTC TAATCCATTA 
ATTGTTGACT TTGCTTGTCT TAATACACAC AAGACATGTA AAGAAAAGTG TCCGGAGTCT 
TCCTTGCCCA AGACAGTCCT TTCTTGCAAA AGGCATAATG GTGTTGAATG TTTTTGTGAT 
GATGATGAAA GTGGTCCTAG AAATGAGGCT GGAGCTTCTT CGAATAACAT TGAACTTGGG 
AATGATTCAG TCATTCAAAT AAACGAGACG GTCATTGGAG ATTGTGTCCT ATATGATGAC 
GATAAAACCA AGAAAGTTCA CCCAATACTT GGTGAAGACA CACAATGGGA AATTCAATGG 
GAGGATCTTG TCGTTGGCGA GCGTATTGGT ATTGGTTCTT ACGGTGAAGT TTACCGTGCT 
GATTGCAATG GCACTGAAGT TGCTGTAAAG AAGTTTCTAG ACCAAGACGT CTCTGGCGAT 
GCACTAGATC AGTTCAAATC GGAAATTGAG ATTATGCTAA GGCTGCGGCA TCCTAATGTT 
GTGCTCTTCA TGGGAGCAAT TACTCGTCCT CCTCATTTCT CTATCTTGAC AGAGTTTCTT 
CCTAGAGGCA GTTTATATAG GATATTGCAT CGTCCCAATC TTGTACTTGA TGAGAAGAGG 
CGGTTGCGTA TGGCTCTTGA TGTGGCTAAG GGAATGAATT ACTTGCACAC CAGCCATCCT 
CCCGTTGTCC ATCGAGATTT AAAGACTCCA AACCTTCTTG TTGACAGAAA TTGGGTTGTA 
AAGGTAATAG TCATCATACT TAATTTCTTT GTCTTCGGAA TTTACCTCAC AATAGCTGAA 
TATAAAAGTA ACATCAAATC AATTGAGTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11414513 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム