VFPFJ_00912 cDNA ORF clone, Purpureocillium lilacinum

Gene
Clones
gRNAs

Summary

Gene Symbol	VFPFJ_00912
Entrez Gene ID	28883046
Full Name	ribosome biogenesis protein
Gene Type	protein-coding
Organism	Purpureocillium lilacinum

mRNA and Protein(s)

mRNA	Protein	Name
XM_018317997.1	XP_018183522.1	ribosome biogenesis protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Biosynthesis of Antibiotic Leucinostatins in Bio-control Fungus Purpureocillium lilacinum and Their Inhibition on Phytophthora Revealed by Genome Mining.
Wang G, Liu Z, Lin R, Li E, Mao Z, Ling J, Yang Y, Yin WB, Xie B
PLoS pathogens12(7)e1005685(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VFPFJ_00912 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VFPFJ_00912 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPu98731	XM_018317997.1 Latest version!	Purpureocillium lilacinum ribosome biogenesis protein (VFPFJ_00912), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VFPFJ_00912 ( XM_018317997.1 ) cDNA ORF clone, Purpureocillium lilacinum -> XP_018183522.1 Purpureocillium lilacinum ribosome biogenesis protein (VFPFJ_00912), partial mRNA.

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CloneID	OPu98731
Clone ID Related Accession (Same CDS sequence)	XM_018317997.1
Accession Version	XM_018317997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Purpureocillium lilacinum
Product	ribosome biogenesis protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_017264030). COMPLETENESS: incomplete on both ends.

ATGGAGCAGC AAGTCCACAA GCCGCACCGC AAGTCCAAGG AGAAGAAGCA GAAGCACACG 
GGGGACCACA ACCCCAAGGC TTTTGCCTTT TCCAAGCCGG GCAAGCTAGC GAAAACTGCC 
GCGCGGTCGC AAGATATCAA AGAACGGCGC TTCCATGTCC CCCAGGTCGA CCGCCTCCCC 
GATGAGGCCC CCCCGCGGCT CGTGGCCATC GTCGGCCCCC CCGGCGTCGG AAAGACGACT 
CTCCTTAAGT CCCTGATCCG ACGATATGCG AAAGAGACCA TCTCGGACCC CCAAGGTCCC 
ATCACCGTCG TCACGTCCAA GAAGCAGCGC CTGACGTTCA TCGAGTGCCC CAACGAGCTC 
GAAGCCATGG TCGACATCGC CAAGGTCGCC GACATTGTCC TCCTCATGAT TGACGGCAAC 
TACGGCTTCG AGATGGAGAC GATGGAGTTC CTCAACGTCC TGGCCTCGAC GGGCATGCCG 
GGCAACGTCT TTGGCATCCT CACACATCTC GATCTGTTCC GCAAGCCGCA GGCGCTCAAG 
GACGCAAAGA AGCGGCTGAA GCGCCGGCTC TGGACGGAGC TGTACCAAGG CGCGCATCTG 
TTTTATCTTT CTGGTGTCAT GAACGGCCGA TACCCCGATC GCGAAATCCA CAACCTCTCG 
CGCTTCCTCT CCGTCATGAA GAACCCGCGT CCCCTCATCT GGCGCAACTC TCACCCATAC 
ACCATCATCG ACAGCTTCCG CGACATCACT CACCCTGCCA ACATCGAGAA AGACCCGAAG 
TGCGATCGGT CCATTGTTCT GTCGGGGTAC TTGCGCGGCA CGAATTTTGC GGCACAAGAT 
CAGCGTGTCC ATGTCCCTGG ACTGGGTGAC TTTACCGTCG CGAACATGGA GGTGCTTCCG 
GATCCGTGCC CCACACCCGC CATGGAGCAG GCGCTCGCCA AAATCACAGG CAAGACTGGG 
CGCAGGAGAC TGGACGAGAA GGAGAAGAAG CTCCACGCTC CCATGTCGGA CCGAAGCGGC 
CTGAAGATCG ATGGCGACGC AATCTGGATC ACAAGAGAGA AGGGCTTCAA CTTCGACAAG 
GACAACGATG ACGAAGAGCG TGGCGAAGGC GAGGAGATGA TTGTGGGACT TCAAGCCGAG 
CGGAAGCTCC TGGGTCAGAC GGACGAAGGC GTGCAGCTGT TTTCTGGCGG CGAGAAGCTG 
AAAACCATCG CCGAGGAGGA AGACACGGGC AGGAAATCTC AAAGAAAGGC GCGATTTGCG 
GACCGGGAAG AAGACGACGA CGAAGATGCC GAGGATGACG AGGGCTTTGT CAGCGGCAGC 
GGCGACGAGG GCTCAGACGT CGATGACGAC GAGTTCAGCG AAGCCAAGGT CGGCAAGCTC 
TTTCGCAAGG ATGCCGAAAA GGCTGATGGC GACGAAGACA TCGCCTTTGC GGACAGCGAC 
TCTGACTTGG GCTCAATCGA TGGCCTTGAC GAGGTGGAGG ATGACGAAGA CTACGATTCC 
GATGAGGAGG CTGCTGCTCT GAGATGGAAA GACGACTTGA CTGAGCGGGC AAGAATACTC 
AACAATAGGC GGCGTTCATA CCACACAGGC GACCTGGCGA GACTCATGTA CGACACGTCA 
TTGAGCGCGG AAGAGGCGCT TAAGCGATGG CGCGGCGACC ATGGCGAGTC AGAAGAGGAA 
GACATTGAAA AGGACTCGGA CGACGAGGAC TTCTTCAAGA AGGCGGCACA GGAAAAGGAG 
GATATGATCG AGGACAGGTC AATACCCCTC TACGACTACG AGGACCTGGC CGCGAAGTGG 
GCCGAGAGGG ACACCGTCGA CAAGCTGCGC AAGAGATTCA CGACTGCTTC ACTGGGCAGG 
GATGATGAGG ACGGCGACGA CGATGAAGGC GACGATGACG GAGACGACGA GAGCGACGAA 
GGCGACGGTG TCTTTGAGGA TCTGGAGGCT GCCGACACCG AGGCCCCCGC GCAACCATCG 
GCGGAGGACA TCGCCGCAGA GCGCGAAAAG AACGCCAAGA GGAAAGAGGA GCTCAAGATG 
CGCTTCGAGG AGGAGGACCG CGAAGGTTTC CTCAACGACA AGGCCAATGC CCGGCGCGAA 
GGCGGCGACA TCCAAGAGTT TGGCGAGGAC GAGTGGTACG ATGCGCAAAA GGCCATGATC 
CAGAAGCAGC TCGACATCAA CAAGGAAGAG TTTGAGACGC TGGACGAGCG GCAACGGGCG 
GCCGTCGAGG GATACCGGGC AGGCAAATAC GCCAAGATTG TCCTCGAGAA CGTGCCGGCC 
GAGTTCGTCA CCAAGTTCAA CGCCAGATTG CCCATCATCG TGGGGGGCCT GACTCCGACC 
GAGGACCGAT GGGGCTTCGT GCAGGTACGC ATCAAGCGGC ATCGATGGCA CAAGAAGATC 
CTCAAGACCA ACGACCCCCT CATCTTCTCC CTGGGCTGGA GGCGGTTCCA GACCCTGCCC 
ATCTACAGCA TCTCGGACTC GCGGACGCGA AACCGCATGC TCAAGTACAC GCCCGAGCAC 
ATGCACTGCT TCGGTACCGT CTACGCGCCG CTCATTGCGC CCAACACTGG CTTTGTCTGC 
TTCAACAGCT TCTCCCCCGA GACCCCAGGC TTCAGGATAG CTGCGACGGG CACGGTGCTC 
AGCGTGGACG AGTCGACCGA GATTGTCAAG AAGCTCAAGC TCACGGGCGT GCCGTACAAG 
ATCTTCAAGA ACACGGCCTT TATCAAGGAC ATGTTCAACT CGTCGCTGGA GATTGCCAAG 
TTTGAGGGCG CGTCCATCAA GACGGTGTCC GGCGTGCGTG GTCAGATCAA GCGCGCGCTG 
TCGAAACCCG AGGGCCACTT CCGCGCGACG TTTGAGGACA AGATCCTGCT CAGCGACATT 
GTCTTCCTGC GGGCGTGGTA CCCCATCAAG CCGCATCGGT TCTACAACCC GGCGTCCAAC 
CTGGTCGGGT GGCAGCCGAT GCGCCTCACG GGCGAGGTGC GGCGGGACGA GGGCGTGGCG 
ACGCCGCAGC TCAAGAACAG CCAGTACCGC AAGATCGAGC GCGAGAACCG GCACTTCAAC 
CCGCTGCGGG TGCCGCGGGC GCTGGCGGCG GAGCTGCCGT ACAAGTCGCA GATTGTGCAG 
ACCAAGAAGC AGAAGCGCGA GACGTACATG CAGAAGCGGG CGGTGGTGCT GGCGCCGGGG 
TCCGAGGAGA AGAAGGCGCG GGCGCTGATG CAGCAGCTGC TGACGGTGCG CAACGACGCG 
GCGGCGAAGC GGCGGGCCAA GAAGGACGAG AACCGCGCCG CCTTCAAGAA GAAGATGGCG 
GACAACCTGG AGAAGAAGGA GGCGCGGGAG AAGCGCGAGA GCAAGGAGTT TTGGCGCAAG 
AACGGGCGCA AGAGGGGCGC GGGCGAGGAG GGCGGCGGCG GCGGCAAGAG GCGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018183522.1
CDS	1..3420
Translation	MEQQVHKPHRKSKEKKQKHTGDHNPKAFAFSKPGKLAKTAARSQDIKERRFHVPQVDRLPDEAPPRLVAIVGPPGVGKTTLLKSLIRRYAKETISDPQGPITVVTSKKQRLTFIECPNELEAMVDIAKVADIVLLMIDGNYGFEMETMEFLNVLASTGMPGNVFGILTHLDLFRKPQALKDAKKRLKRRLWTELYQGAHLFYLSGVMNGRYPDREIHNLSRFLSVMKNPRPLIWRNSHPYTIIDSFRDITHPANIEKDPKCDRSIVLSGYLRGTNFAAQDQRVHVPGLGDFTVANMEVLPDPCPTPAMEQALAKITGKTGRRRLDEKEKKLHAPMSDRSGLKIDGDAIWITREKGFNFDKDNDDEERGEGEEMIVGLQAERKLLGQTDEGVQLFSGGEKLKTIAEEEDTGRKSQRKARFADREEDDDEDAEDDEGFVSGSGDEGSDVDDDEFSEAKVGKLFRKDAEKADGDEDIAFADSDSDLGSIDGLDEVEDDEDYDSDEEAAALRWKDDLTERARILNNRRRSYHTGDLARLMYDTSLSAEEALKRWRGDHGESEEEDIEKDSDDEDFFKKAAQEKEDMIEDRSIPLYDYEDLAAKWAERDTVDKLRKRFTTASLGRDDEDGDDDEGDDDGDDESDEGDGVFEDLEAADTEAPAQPSAEDIAAEREKNAKRKEELKMRFEEEDREGFLNDKANARREGGDIQEFGEDEWYDAQKAMIQKQLDINKEEFETLDERQRAAVEGYRAGKYAKIVLENVPAEFVTKFNARLPIIVGGLTPTEDRWGFVQVRIKRHRWHKKILKTNDPLIFSLGWRRFQTLPIYSISDSRTRNRMLKYTPEHMHCFGTVYAPLIAPNTGFVCFNSFSPETPGFRIAATGTVLSVDESTEIVKKLKLTGVPYKIFKNTAFIKDMFNSSLEIAKFEGASIKTVSGVRGQIKRALSKPEGHFRATFEDKILLSDIVFLRAWYPIKPHRFYNPASNLVGWQPMRLTGEVRRDEGVATPQLKNSQYRKIERENRHFNPLRVPRALAAELPYKSQIVQTKKQKRETYMQKRAVVLAPGSEEKKARALMQQLLTVRNDAAAKRRAKKDENRAAFKKKMADNLEKKEAREKRESKEFWRKNGRKRGAGEEGGGGGKRRK

Target ORF information:

RefSeq Version	XM_018317997.1
Organism	Purpureocillium lilacinum
Definition	Purpureocillium lilacinum ribosome biogenesis protein (VFPFJ_00912), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018317997.1

ORF Insert Sequence:

ATGGAGCAGC AAGTCCACAA GCCGCACCGC AAGTCCAAGG AGAAGAAGCA GAAGCACACG 
GGGGACCACA ACCCCAAGGC TTTTGCCTTT TCCAAGCCGG GCAAGCTAGC GAAAACTGCC 
GCGCGGTCGC AAGATATCAA AGAACGGCGC TTCCATGTCC CCCAGGTCGA CCGCCTCCCC 
GATGAGGCCC CCCCGCGGCT CGTGGCCATC GTCGGCCCCC CCGGCGTCGG AAAGACGACT 
CTCCTTAAGT CCCTGATCCG ACGATATGCG AAAGAGACCA TCTCGGACCC CCAAGGTCCC 
ATCACCGTCG TCACGTCCAA GAAGCAGCGC CTGACGTTCA TCGAGTGCCC CAACGAGCTC 
GAAGCCATGG TCGACATCGC CAAGGTCGCC GACATTGTCC TCCTCATGAT TGACGGCAAC 
TACGGCTTCG AGATGGAGAC GATGGAGTTC CTCAACGTCC TGGCCTCGAC GGGCATGCCG 
GGCAACGTCT TTGGCATCCT CACACATCTC GATCTGTTCC GCAAGCCGCA GGCGCTCAAG 
GACGCAAAGA AGCGGCTGAA GCGCCGGCTC TGGACGGAGC TGTACCAAGG CGCGCATCTG 
TTTTATCTTT CTGGTGTCAT GAACGGCCGA TACCCCGATC GCGAAATCCA CAACCTCTCG 
CGCTTCCTCT CCGTCATGAA GAACCCGCGT CCCCTCATCT GGCGCAACTC TCACCCATAC 
ACCATCATCG ACAGCTTCCG CGACATCACT CACCCTGCCA ACATCGAGAA AGACCCGAAG 
TGCGATCGGT CCATTGTTCT GTCGGGGTAC TTGCGCGGCA CGAATTTTGC GGCACAAGAT 
CAGCGTGTCC ATGTCCCTGG ACTGGGTGAC TTTACCGTCG CGAACATGGA GGTGCTTCCG 
GATCCGTGCC CCACACCCGC CATGGAGCAG GCGCTCGCCA AAATCACAGG CAAGACTGGG 
CGCAGGAGAC TGGACGAGAA GGAGAAGAAG CTCCACGCTC CCATGTCGGA CCGAAGCGGC 
CTGAAGATCG ATGGCGACGC AATCTGGATC ACAAGAGAGA AGGGCTTCAA CTTCGACAAG 
GACAACGATG ACGAAGAGCG TGGCGAAGGC GAGGAGATGA TTGTGGGACT TCAAGCCGAG 
CGGAAGCTCC TGGGTCAGAC GGACGAAGGC GTGCAGCTGT TTTCTGGCGG CGAGAAGCTG 
AAAACCATCG CCGAGGAGGA AGACACGGGC AGGAAATCTC AAAGAAAGGC GCGATTTGCG 
GACCGGGAAG AAGACGACGA CGAAGATGCC GAGGATGACG AGGGCTTTGT CAGCGGCAGC 
GGCGACGAGG GCTCAGACGT CGATGACGAC GAGTTCAGCG AAGCCAAGGT CGGCAAGCTC 
TTTCGCAAGG ATGCCGAAAA GGCTGATGGC GACGAAGACA TCGCCTTTGC GGACAGCGAC 
TCTGACTTGG GCTCAATCGA TGGCCTTGAC GAGGTGGAGG ATGACGAAGA CTACGATTCC 
GATGAGGAGG CTGCTGCTCT GAGATGGAAA GACGACTTGA CTGAGCGGGC AAGAATACTC 
AACAATAGGC GGCGTTCATA CCACACAGGC GACCTGGCGA GACTCATGTA CGACACGTCA 
TTGAGCGCGG AAGAGGCGCT TAAGCGATGG CGCGGCGACC ATGGCGAGTC AGAAGAGGAA 
GACATTGAAA AGGACTCGGA CGACGAGGAC TTCTTCAAGA AGGCGGCACA GGAAAAGGAG 
GATATGATCG AGGACAGGTC AATACCCCTC TACGACTACG AGGACCTGGC CGCGAAGTGG 
GCCGAGAGGG ACACCGTCGA CAAGCTGCGC AAGAGATTCA CGACTGCTTC ACTGGGCAGG 
GATGATGAGG ACGGCGACGA CGATGAAGGC GACGATGACG GAGACGACGA GAGCGACGAA 
GGCGACGGTG TCTTTGAGGA TCTGGAGGCT GCCGACACCG AGGCCCCCGC GCAACCATCG 
GCGGAGGACA TCGCCGCAGA GCGCGAAAAG AACGCCAAGA GGAAAGAGGA GCTCAAGATG 
CGCTTCGAGG AGGAGGACCG CGAAGGTTTC CTCAACGACA AGGCCAATGC CCGGCGCGAA 
GGCGGCGACA TCCAAGAGTT TGGCGAGGAC GAGTGGTACG ATGCGCAAAA GGCCATGATC 
CAGAAGCAGC TCGACATCAA CAAGGAAGAG TTTGAGACGC TGGACGAGCG GCAACGGGCG 
GCCGTCGAGG GATACCGGGC AGGCAAATAC GCCAAGATTG TCCTCGAGAA CGTGCCGGCC 
GAGTTCGTCA CCAAGTTCAA CGCCAGATTG CCCATCATCG TGGGGGGCCT GACTCCGACC 
GAGGACCGAT GGGGCTTCGT GCAGGTACGC ATCAAGCGGC ATCGATGGCA CAAGAAGATC 
CTCAAGACCA ACGACCCCCT CATCTTCTCC CTGGGCTGGA GGCGGTTCCA GACCCTGCCC 
ATCTACAGCA TCTCGGACTC GCGGACGCGA AACCGCATGC TCAAGTACAC GCCCGAGCAC 
ATGCACTGCT TCGGTACCGT CTACGCGCCG CTCATTGCGC CCAACACTGG CTTTGTCTGC 
TTCAACAGCT TCTCCCCCGA GACCCCAGGC TTCAGGATAG CTGCGACGGG CACGGTGCTC 
AGCGTGGACG AGTCGACCGA GATTGTCAAG AAGCTCAAGC TCACGGGCGT GCCGTACAAG 
ATCTTCAAGA ACACGGCCTT TATCAAGGAC ATGTTCAACT CGTCGCTGGA GATTGCCAAG 
TTTGAGGGCG CGTCCATCAA GACGGTGTCC GGCGTGCGTG GTCAGATCAA GCGCGCGCTG 
TCGAAACCCG AGGGCCACTT CCGCGCGACG TTTGAGGACA AGATCCTGCT CAGCGACATT 
GTCTTCCTGC GGGCGTGGTA CCCCATCAAG CCGCATCGGT TCTACAACCC GGCGTCCAAC 
CTGGTCGGGT GGCAGCCGAT GCGCCTCACG GGCGAGGTGC GGCGGGACGA GGGCGTGGCG 
ACGCCGCAGC TCAAGAACAG CCAGTACCGC AAGATCGAGC GCGAGAACCG GCACTTCAAC 
CCGCTGCGGG TGCCGCGGGC GCTGGCGGCG GAGCTGCCGT ACAAGTCGCA GATTGTGCAG 
ACCAAGAAGC AGAAGCGCGA GACGTACATG CAGAAGCGGG CGGTGGTGCT GGCGCCGGGG 
TCCGAGGAGA AGAAGGCGCG GGCGCTGATG CAGCAGCTGC TGACGGTGCG CAACGACGCG 
GCGGCGAAGC GGCGGGCCAA GAAGGACGAG AACCGCGCCG CCTTCAAGAA GAAGATGGCG 
GACAACCTGG AGAAGAAGGA GGCGCGGGAG AAGCGCGAGA GCAAGGAGTT TTGGCGCAAG 
AACGGGCGCA AGAGGGGCGC GGGCGAGGAG GGCGGCGGCG GCGGCAAGAG GCGGAAGTAG