plekhg4b cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	plekhg4b
Entrez Gene ID	101078082
Full Name	pleckstrin homology and RhoGEF domain containing G4B
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011605704.1	XP_011604006.1	pleckstrin homology domain-containing family G member 4B isoform X3
XM_011605704.2	XP_011604006.2	pleckstrin homology domain-containing family G member 4B isoform X3
XM_011605705.1	XP_011604007.1	pleckstrin homology domain-containing family G member 4B isoform X4
XM_011605702.2	XP_011604004.2	pleckstrin homology domain-containing family G member 4B isoform X1
XM_011605703.2	XP_011604005.2	pleckstrin homology domain-containing family G member 4B isoform X2
XM_011605703.1	XP_011604005.1	pleckstrin homology domain-containing family G member 4B isoform X2
XM_011605702.1	XP_011604004.1	pleckstrin homology domain-containing family G member 4B isoform X1

Pathways from BioSystems
Gene Ontology

The following plekhg4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plekhg4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo69457	XM_011605702.2 Latest version!	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo69458	XM_011605703.2 Latest version!	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo69459	XM_011605704.2 Latest version!	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo30836	XM_011605702.1 Latest version!	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo30837	XM_011605703.1 Latest version!	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo30838	XM_011605704.1 Latest version!	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo30839	XM_011605705.1 Latest version!	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plekhg4b ( XM_011605702.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604004.2 Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X1, mRNA.

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CloneID	OTo69457
Clone ID Related Accession (Same CDS sequence)	XM_011605702.2
Accession Version	XM_011605702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	pleckstrin homology domain-containing family G member 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCA 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CAAACAAGAC CTCTCATATA GCAGTAGGCC TCTCTACTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604004.2
CDS	249..5465
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGASKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITANKTSHIAVGLSTVV

Target ORF information:

RefSeq Version	XM_011605702.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605702.2

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCA 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CAAACAAGAC CTCTCATATA GCAGTAGGCC TCTCTACTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605703.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604005.2 Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X2, mRNA.

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CloneID	OTo69458
Clone ID Related Accession (Same CDS sequence)	XM_011605703.2
Accession Version	XM_011605703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	pleckstrin homology domain-containing family G member 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCACTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604005.2
CDS	250..5463
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGASKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITANKTSHIAVGLSTVV

Target ORF information:

RefSeq Version	XM_011605703.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605703.2

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCACTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605704.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604006.2 Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo69459
Clone ID Related Accession (Same CDS sequence)	XM_011605704.2
Accession Version	XM_011605704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	pleckstrin homology domain-containing family G member 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011605704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCA 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604006.2
CDS	249..5426
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGASKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITAL

Target ORF information:

RefSeq Version	XM_011605704.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes pleckstrin homology domain-containing family G member 4B (LOC101078082), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605704.2

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAT 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCGC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGCTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCA 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605702.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604004.1 Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X1, mRNA.

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CloneID	OTo30836
Clone ID Related Accession (Same CDS sequence)	XM_011605702.1
Accession Version	XM_011605702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	pleckstrin homology domain-containing family G member 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCG 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CAAACAAGAC CTCTCATATA GCAGTAGGCC TCTCTACTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604004.1
CDS	294..5510
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGPSKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITANKTSHIAVGLSTVV

Target ORF information:

RefSeq Version	XM_011605702.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605702.1

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCG 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CAAACAAGAC CTCTCATATA GCAGTAGGCC TCTCTACTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605703.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604005.1 Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X2, mRNA.

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CloneID	OTo30837
Clone ID Related Accession (Same CDS sequence)	XM_011605703.1
Accession Version	XM_011605703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	pleckstrin homology domain-containing family G member 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCGCTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604005.1
CDS	294..5507
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGPSKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITANKTSHIAVGLSTVV

Target ORF information:

RefSeq Version	XM_011605703.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605703.1

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCGCTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605704.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604006.1 Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X3, mRNA.

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CloneID	OTo30838
Clone ID Related Accession (Same CDS sequence)	XM_011605704.1
Accession Version	XM_011605704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	pleckstrin homology domain-containing family G member 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCG 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604006.1
CDS	266..5443
Translation	MLLLGKMHSRAKSRSCDNYLSIKDAESLDSCIQSTLSALYRPFGVTAATVLWQLFSVVERQYRGDGLRCLIDFLLPSKRILQLIQQETCVRFRGLLFYHEGWPLCIHEKVILQLAPLHKVRLKQGDFYLQIVPLGRKAAKLVIKCLSASGQAITEIPITESMYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGPSKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITAL

Target ORF information:

RefSeq Version	XM_011605704.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605704.1

ORF Insert Sequence:

ATGCTGTTGC TGGGCAAAAT GCACTCCAGA GCAAAGTCCC GAAGCTGTGA CAACTACCTG 
AGCATTAAGG ACGCTGAGTC TTTGGACAGT TGTATCCAGA GCACCTTGTC GGCTCTGTAC 
CGACCCTTCG GCGTCACCGC TGCTACCGTC CTGTGGCAGC TCTTCAGCGT GGTGGAAAGG 
CAGTACAGGG GAGACGGCCT CCGCTGCCTC ATTGACTTCT TGCTGCCCTC CAAAAGGATA 
CTGCAGCTCA TCCAGCAGGA AACCTGTGTG AGGTTCAGAG GATTGCTGTT TTACCATGAA 
GGCTGGCCTC TCTGCATCCA CGAGAAGGTC ATCCTGCAGC TCGCCCCTCT GCACAAAGTG 
CGACTAAAGC AAGGAGACTT CTACCTCCAG ATTGTTCCGC TGGGTCGCAA GGCTGCCAAA 
CTGGTGATAA AATGCTTGTC GGCCAGCGGA CAAGCCATCA CGGAGATCCC CATCACGGAG 
AGCATGTACG GCAGCGTCTT CACAGCTGAG TTTCTGCAGA ATGTAACTCG GGACCGTAAC 
CTGCACCCGC TCCAGAACTG CCTGCTCACC ACCGGCACAG CCGTGTACAG GACGCCGTGG 
AAGAACGTGG TCAACCCACT GTTTGTCAGC AGCACGTCAG ACACCATCAT GCAAGCGCGC 
TGCAGCAGAG GCGCCTTCCG TGGTCATCTC AGCACCTGCA GCACCAGCGG ATCCACCGGG 
ACCCTGGACA GCCACCGCAG CTCGATAGAG TCGCTGCACT CTCAGGGGAC TGATTCTATC 
TTCTCTGAGC CCACCTCCCC AAACAGAAAC CCCATGGACC CAAATGGTGA GGTCCATGGT 
ACCAGTGATA CTGCAGTACC TATAATTAAA CTTGAAAGGT CTGGTGAAGA GTGTGAGACA 
GGTCAGGAGT CCGAAGACAA TGATGGGAGA GAGGGGGGGC CTGGGTCCAA GATGCTCTCT 
TTCACCACAG ACCTTAGCAA CCCAGGATGT CGTCGCCGTC CCCCAAGGGA CTCTGTAGCA 
TTTGAGAGCA GGAGACTTTT CAGGAAGTCT TACATGGAAG CCCTGCAGAA CCCCATGAGC 
CTTGGGTCCA GCTCTGAATC CATTCTGGAG GAACAGTCTG AGCACAGCAT TGCCCTGGGA 
GATAGAGCAG TGACACCTGG CAGCAGCCCT GAGGCCAGAA CTCCTTCTAG AGAACTCTTG 
TCGCGAAGGC TGGTTGGTCG GGGGTGGCTC AGCAGCGATG AATCCAGGCC CAGCACACCT 
TTGCTGTATT TACAAAGGGG GTTGAGGAGC GCTGAGAGGC GGGCGGAGAG ACGTTCAAAG 
TCACTGGAGA GAACAAACAA AGGCGGCCAC GTTAAAGGCC ACCGGGAAAG ATCATCCTCC 
GGAGGCACCG TCAACATTTC CCCGAAAAAA CTGATGAACG GCTATGCTCT GCGTTTCGGA 
AAGCTAGACG TCGAGGCGGC ACTTCCTGGG TCGGAGAGAA GGACCAACAA GGAGGAGTCA 
GGCCAGCGTG GCGACGGCGT CAGCGGCGAG GGCAGGCGAC ACAGACTCAT CAGCGAGGAT 
TCACACTCTC CTAAAAGTGC CAATGGATCA GCCATCACGC TGGCGCCCGT AACAAGGCCT 
TCATGCGAGA GCAGCTCAGC TCTCCCCAAA CTGGTGTCAG AGGTCAATCA GGAGCTCTTC 
ACATCGGGGG CCGTGATCCT GCCAGGAAGC AGAGATCGTG GTGGGAGGGC GGTGCTGCAG 
GTGTGCGCCA GAGCTCAGGT GTGGGCCGGG GAGAGTTGCA CGGTCAATGA CCTCACCTGT 
TTACTTGGTT ACTACTACTC CACACTGCGG AAGGAAAGGC GAGATCAAGG CCTGTCTGTT 
GTAATAGACG GCAGGAGGCA GCAGCCTGTT CCAGCTCTGT TGTTGTCTCT GTCTGAGTTG 
CAGGCATTGG TACCAAATGC ACTTTATTCG GTTCTCTTCC TGGTGGACAA AGAGACAGTG 
GCCAAACCAG AGAGAGACAT GAATGTGCAG ACTGAAACGT TGACATCTCT AAAAGCCTTG 
CTGAAGCACA TTGACCAAAA CCAGCTCACG CGAGACCTGG ACGGCACCTT CCACTACGAC 
CACAGCCACT GGATCCACTT CAGGCAGAAA ATCGAACCCT TCGCCAGCAG CTGTTCTGCA 
GCTATCTCCT CCCTGCAGCA GTCGATCGAC TCACTGGGCA ACAGCGGCAA CTTAAAGACG 
TCAAAGGAAG TGTCTGAGGT GCTGGAGCAG CAGCGACACC TCATGAAGAA GGTACTGGAC 
GACACGCAGC TAAACCGGCT GCGTCTAGAA GGAGGCACCG TCCTGGCCCG CATCAGGAAA 
GAAGAAGCCT GTGAGAATGA AAACTACAAA GATGCCATTG ACATGTTAAG TGCGCTGTAC 
AACCAAGTAG ACGAAGAAGT CCACAAACTT GTGATTCTGT CCAACAAGTG TCAGAAGGAG 
CTGGAGAGCC TGCTGGAGGT GCGCATGTTT GAAGAGCAAA CACAGCAGAT CAAAGTCTGG 
TTCAGCAGCG AGGGAGAGAA GCAGCTCGTA CCTCTGGAAT CACAAACTCT GTCGGTGGCC 
AAAATCAAGG AGATGAGAGC CAGTTTGGAG CACTTTCTGG AGGAGTCCGT GCACCAGCAG 
AGACATGGTC TGCAGCTGGT GAAGGAGTCC CCAGAGTCCC TTCCAGGCTC TGCCCTGATG 
GACTTTAAAC AACATCTGGG CTCCATTCTG AGCCGAGTAG AGAAAAGGAA GGACCACTTA 
GAAATCCTTA CAAATCTCTA TGAATTCTAT GACTCAGTGA ACAAGTGGAT GGAGCACTGC 
CAGGATTATT TCCATCAGCT GAGTCTGGAC AGCACAAGCT TGATTCTGTC GCCCTCTGTG 
GAGCAGACCC TCCAGGACTA CTACGCAGAA GCGTCCAAGT TTTCCATGGA TAACTTCAGC 
ACCCTCAACA ACATGGTGCT CTCCCTGGAA AGCCCTCAGC AGCTGCAGCA CTGGAACTCT 
GTGTGGCATA AATGCCAACA AACCAAACAG CAGCTGGAAG AAATTCTGGC CCGAGCCACG 
ACGGCCCAGG ACCCCACGGC CACCGACGTA AAGACCCCAG AGAGTGGCCC CAGCAAACCA 
GAGCAGCACG GAGCGAACGC GTGCGTGCTG CTACCTTTTG AGGACAGCAA GCCTCATTCT 
TCTGAGCCTT ACTCCAAGAG CCCCACCAGC TCCATCTCCT CCTCCTCGCA CTTCATTTTC 
CCCTCTGACT GTGACAGCAA ACTGAGGCAA AGTCCGTCCA TGTTTGAGGA CACGGACAGC 
GACTGCACCG TGGACTCGTC CGTCTCCTGC CACTCGGAGC CCATCTACAC CGGCACCGCC 
CGCCTCCGCA AGCACCCCAT GAAGAAGATC ATGAAGAAGA CCGTGAGCTA TGAGCTGAAC 
GCAAGGGACA GCGTCCACTC GGATGCCGGC CACATTCACG GCTACACGGG CGTGTACATC 
AAGGGTTTGG AGGTGACGAA CAACGTGTCC GCGGAGAAGA AGTTGCAGAG GCCCGACGTG 
ACGAGCCCGG CTTTGGGACG CAGCCGTAGC ATGTCCACAC CGTCCAGGGC GCACAGCAGA 
CACAGTGACG GAGATATTAA GAAACAGAGC AGCAGCAAAG TGCAGCACAT CATGGACGAG 
ATGATTTCCA CGGAGCGGGA GTACGTCCGC TCCCTCAGCT ACATCATCGA GCAGTATTTC 
CCAGAGATGG AGCGGCTGGA TTTGCCCCAG GACCTGCGAG GGAAGCGCAG CATCATTTTT 
GGAAACGTGG AGAAGCTGTG GGACTTTCAC ACTCAGTACT TCCTGAAGGA TCTGGAGTCG 
TGTGCTCACT GTCCACTCTC GATCAGCAGC TGTTTTCTCA GACATGAGGA TCAGTTTGGA 
ATGTATGCCC TCTACAGCAA GAATAAGCCG CAGTCTGACG CTCTGCTCAG CAGCCACGGG 
AATGAATTCT TCAAGAACAA ACAGATCGAG CTGGGCGACA AGATGGACTT AGCGTCCTAC 
TTGCTGAAGC CCATCCAGAG GATGAGTAAA TATGCTCTGC TGCTCAAAGA CCTGATCAAG 
GAGTGCAGTC AGTCTCAGGA GCAGGAGCTG AGTGACCTCC GCACGGCAGA GGACATGGTC 
AAATTTCAGC TTCGCCATGG CAATGACCTG CTGGCTATGG ATGCTATTCG TGGATGTGAT 
GTGAACCTGA AAGAGCAGGG TCAGCTCTGC TGCCAGGACG AGTTCATCGT CTGGTGCGGG 
AGGAGGAAGT ACCTCCGCCA TGTCTTCTTG TTTGAAGACC TCATCCTGTT CAGCAAGACC 
AAAAAGATCG AAGGAGGATA TGACATTTAC ATCTACAAAC AGTCTTTCAA GACGGCAGAG 
ATCGGTATGA CGGAGAACGT CGGGGACAGC GGCCTACGCT TCGAGATTTG GTTCCGCAGG 
AGGAAGTCAC AGGACACCTT CATACTCCAA GCCAGCAGTG CGGAGGTCAA GGCCGTGTGG 
ACAGCCATCA TTGGGAAGAT CCTGTGGAGA CAGGCGCTCA GAAATCGAGC CTTGCGCATG 
CAGGAGATGG TGTCCATGGG AATTGGAAGT AAGCCCTTCA TGGACATTAA GCCAAGCGAT 
GCGGCCATCA GCGACAGGGC CATTGATTAT ATCATGAAGG GGACAGAAGC CAGGACCAGG 
GCGTCCATTG CTGTGCCTTC CTTTGAACAC GCCACTTCCT TCAAGCGACC CCACTCCACC 
ATCTCCAACA GCAGCACCTC CTCCTCCAGC AGCCAGTCCT CCTCCTCGTT GCTGGGCTCG 
CTCAACCTCC ATCTTTACTC CTCCCCCTCT CTCCCACACA CACATCCGTA CCCGATGACC 
AACATCCCCT CCTTTGCTCA GTGGCCGTAC GACTGCATCG AGGAGGATGA GCTGGAGCAG 
GACAGTGGGA GTCAACCCTC TATGACCACT GAGAGCTCGG AGACCTCGTC GCAGTGCACC 
TCCAGTGACA GCGTGACTGG GCTGAGCACG CTGACGATAG CTGTGCATCC CAGCATCACC 
ATGGACTCTT TCAACAACAA CGAATCCTCG TCTTTCCTGT GCTCCTCCAC ACCCTCCTCC 
ACCATGGCTT CTCCTTTAAT GTTCCAAAAG GAGGAAGACC TCCAGCCCAA TGGCACCAAC 
TTCATCACAG CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg4b ( XM_011605705.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011604007.1 Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X4, mRNA.

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CloneID	OTo30839
Clone ID Related Accession (Same CDS sequence)	XM_011605705.1
Accession Version	XM_011605705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	pleckstrin homology domain-containing family G member 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGGCA GCGTCTTCAC AGCTGAGTTT CTGCAGAATG TAACTCGGGA CCGTAACCTG 
CACCCGCTCC AGAACTGCCT GCTCACCACC GGCACAGCCG TGTACAGGAC GCCGTGGAAG 
AACGTGGTCA ACCCACTGTT TGTCAGCAGC ACGTCAGACA CCATCATGCA AGCGCGCTGC 
AGCAGAGGCG CCTTCCGTGG TCATCTCAGC ACCTGCAGCA CCAGCGGATC CACCGGGACC 
CTGGACAGCC ACCGCAGCTC GATAGAGTCG CTGCACTCTC AGGGGACTGA TTCTATCTTC 
TCTGAGCCCA CCTCCCCAAA CAGAAACCCC ATGGACCCAA ATGGTGAGGT CCATGGTACC 
AGTGATACTG CAGTACCTAT AATTAAACTT GAAAGGTCTG GTGAAGAGTG TGAGACAGGT 
CAGGAGTCCG AAGACAATGA TGGGAGAGAG GGGGGGCCTG GGTCCAAGAT GCTCTCTTTC 
ACCACAGACC TTAGCAACCC AGGATGTCGT CGCCGTCCCC CAAGGGACTC TGTAGCATTT 
GAGAGCAGGA GACTTTTCAG GAAGTCTTAC ATGGAAGCCC TGCAGAACCC CATGAGCCTT 
GGGTCCAGCT CTGAATCCAT TCTGGAGGAA CAGTCTGAGC ACAGCATTGC CCTGGGAGAT 
AGAGCAGTGA CACCTGGCAG CAGCCCTGAG GCCAGAACTC CTTCTAGAGA ACTCTTGTCG 
CGAAGGCTGG TTGGTCGGGG GTGGCTCAGC AGCGATGAAT CCAGGCCCAG CACACCTTTG 
CTGTATTTAC AAAGGGGGTT GAGGAGCGCT GAGAGGCGGG CGGAGAGACG TTCAAAGTCA 
CTGGAGAGAA CAAACAAAGG CGGCCACGTT AAAGGCCACC GGGAAAGATC ATCCTCCGGA 
GGCACCGTCA ACATTTCCCC GAAAAAACTG ATGAACGGCT ATGCTCTGCG TTTCGGAAAG 
CTAGACGTCG AGGCGGCACT TCCTGGGTCG GAGAGAAGGA CCAACAAGGA GGAGTCAGGC 
CAGCGTGGCG ACGGCGTCAG CGGCGAGGGC AGGCGACACA GACTCATCAG CGAGGATTCA 
CACTCTCCTA AAAGTGCCAA TGGATCAGCC ATCACGCTGG CGCCCGTAAC AAGGCCTTCA 
TGCGAGAGCA GCTCAGCTCT CCCCAAACTG GTGTCAGAGG TCAATCAGGA GCTCTTCACA 
TCGGGGGCCG TGATCCTGCC AGGAAGCAGA GATCGTGGTG GGAGGGCGGT GCTGCAGGTG 
TGCGCCAGAG CTCAGGTGTG GGCCGGGGAG AGTTGCACGG TCAATGACCT CACCTGTTTA 
CTTGGTTACT ACTACTCCAC ACTGCGGAAG GAAAGGCGAG ATCAAGGCCT GTCTGTTGTA 
ATAGACGGCA GGAGGCAGCA GCCTGTTCCA GCTCTGTTGT TGTCTCTGTC TGAGTTGCAG 
GCATTGGTAC CAAATGCACT TTATTCGGTT CTCTTCCTGG TGGACAAAGA GACAGTGGCC 
AAACCAGAGA GAGACATGAA TGTGCAGACT GAAACGTTGA CATCTCTAAA AGCCTTGCTG 
AAGCACATTG ACCAAAACCA GCTCACGCGA GACCTGGACG GCACCTTCCA CTACGACCAC 
AGCCACTGGA TCCACTTCAG GCAGAAAATC GAACCCTTCG CCAGCAGCTG TTCTGCAGCT 
ATCTCCTCCC TGCAGCAGTC GATCGACTCA CTGGGCAACA GCGGCAACTT AAAGACGTCA 
AAGGAAGTGT CTGAGGTGCT GGAGCAGCAG CGACACCTCA TGAAGAAGGT ACTGGACGAC 
ACGCAGCTAA ACCGGCTGCG TCTAGAAGGA GGCACCGTCC TGGCCCGCAT CAGGAAAGAA 
GAAGCCTGTG AGAATGAAAA CTACAAAGAT GCCATTGACA TGTTAAGTGC GCTGTACAAC 
CAAGTAGACG AAGAAGTCCA CAAACTTGTG ATTCTGTCCA ACAAGTGTCA GAAGGAGCTG 
GAGAGCCTGC TGGAGGTGCG CATGTTTGAA GAGCAAACAC AGCAGATCAA AGTCTGGTTC 
AGCAGCGAGG GAGAGAAGCA GCTCGTACCT CTGGAATCAC AAACTCTGTC GGTGGCCAAA 
ATCAAGGAGA TGAGAGCCAG TTTGGAGCAC TTTCTGGAGG AGTCCGTGCA CCAGCAGAGA 
CATGGTCTGC AGCTGGTGAA GGAGTCCCCA GAGTCCCTTC CAGGCTCTGC CCTGATGGAC 
TTTAAACAAC ATCTGGGCTC CATTCTGAGC CGAGTAGAGA AAAGGAAGGA CCACTTAGAA 
ATCCTTACAA ATCTCTATGA ATTCTATGAC TCAGTGAACA AGTGGATGGA GCACTGCCAG 
GATTATTTCC ATCAGCTGAG TCTGGACAGC ACAAGCTTGA TTCTGTCGCC CTCTGTGGAG 
CAGACCCTCC AGGACTACTA CGCAGAAGCG TCCAAGTTTT CCATGGATAA CTTCAGCACC 
CTCAACAACA TGGTGCTCTC CCTGGAAAGC CCTCAGCAGC TGCAGCACTG GAACTCTGTG 
TGGCATAAAT GCCAACAAAC CAAACAGCAG CTGGAAGAAA TTCTGGCCCG AGCCACGACG 
GCCCAGGACC CCACGGCCAC CGACGTAAAG ACCCCAGAGA GTGGCCCCAG CAAACCAGAG 
CAGCACGGAG CGAACGCGTG CGTGCTGCTA CCTTTTGAGG ACAGCAAGCC TCATTCTTCT 
GAGCCTTACT CCAAGAGCCC CACCAGCTCC ATCTCCTCCT CCTCGCACTT CATTTTCCCC 
TCTGACTGTG ACAGCAAACT GAGGCAAAGT CCGTCCATGT TTGAGGACAC GGACAGCGAC 
TGCACCGTGG ACTCGTCCGT CTCCTGCCAC TCGGAGCCCA TCTACACCGG CACCGCCCGC 
CTCCGCAAGC ACCCCATGAA GAAGATCATG AAGAAGACCG TGAGCTATGA GCTGAACGCA 
AGGGACAGCG TCCACTCGGA TGCCGGCCAC ATTCACGGCT ACACGGGCGT GTACATCAAG 
GGTTTGGAGG TGACGAACAA CGTGTCCGCG GAGAAGAAGT TGCAGAGGCC CGACGTGACG 
AGCCCGGCTT TGGGACGCAG CCGTAGCATG TCCACACCGT CCAGGGCGCA CAGCAGACAC 
AGTGACGGAG ATATTAAGAA ACAGAGCAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCGCTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604007.1
CDS	455..5188
Translation	MYGSVFTAEFLQNVTRDRNLHPLQNCLLTTGTAVYRTPWKNVVNPLFVSSTSDTIMQARCSRGAFRGHLSTCSTSGSTGTLDSHRSSIESLHSQGTDSIFSEPTSPNRNPMDPNGEVHGTSDTAVPIIKLERSGEECETGQESEDNDGREGGPGSKMLSFTTDLSNPGCRRRPPRDSVAFESRRLFRKSYMEALQNPMSLGSSSESILEEQSEHSIALGDRAVTPGSSPEARTPSRELLSRRLVGRGWLSSDESRPSTPLLYLQRGLRSAERRAERRSKSLERTNKGGHVKGHRERSSSGGTVNISPKKLMNGYALRFGKLDVEAALPGSERRTNKEESGQRGDGVSGEGRRHRLISEDSHSPKSANGSAITLAPVTRPSCESSSALPKLVSEVNQELFTSGAVILPGSRDRGGRAVLQVCARAQVWAGESCTVNDLTCLLGYYYSTLRKERRDQGLSVVIDGRRQQPVPALLLSLSELQALVPNALYSVLFLVDKETVAKPERDMNVQTETLTSLKALLKHIDQNQLTRDLDGTFHYDHSHWIHFRQKIEPFASSCSAAISSLQQSIDSLGNSGNLKTSKEVSEVLEQQRHLMKKVLDDTQLNRLRLEGGTVLARIRKEEACENENYKDAIDMLSALYNQVDEEVHKLVILSNKCQKELESLLEVRMFEEQTQQIKVWFSSEGEKQLVPLESQTLSVAKIKEMRASLEHFLEESVHQQRHGLQLVKESPESLPGSALMDFKQHLGSILSRVEKRKDHLEILTNLYEFYDSVNKWMEHCQDYFHQLSLDSTSLILSPSVEQTLQDYYAEASKFSMDNFSTLNNMVLSLESPQQLQHWNSVWHKCQQTKQQLEEILARATTAQDPTATDVKTPESGPSKPEQHGANACVLLPFEDSKPHSSEPYSKSPTSSISSSSHFIFPSDCDSKLRQSPSMFEDTDSDCTVDSSVSCHSEPIYTGTARLRKHPMKKIMKKTVSYELNARDSVHSDAGHIHGYTGVYIKGLEVTNNVSAEKKLQRPDVTSPALGRSRSMSTPSRAHSRHSDGDIKKQSSSKVQHIMDEMISTEREYVRSLSYIIEQYFPEMERLDLPQDLRGKRSIIFGNVEKLWDFHTQYFLKDLESCAHCPLSISSCFLRHEDQFGMYALYSKNKPQSDALLSSHGNEFFKNKQIELGDKMDLASYLLKPIQRMSKYALLLKDLIKECSQSQEQELSDLRTAEDMVKFQLRHGNDLLAMDAIRGCDVNLKEQGQLCCQDEFIVWCGRRKYLRHVFLFEDLILFSKTKKIEGGYDIYIYKQSFKTAEIGMTENVGDSGLRFEIWFRRRKSQDTFILQASSAEVKAVWTAIIGKILWRQALRNRALRMQEMVSMGIGSKPFMDIKPSDAAISDRAIDYIMKGTEARTRASIAVPSFEHATSFKRPHSTISNSSTSSSSSQSSSSLLGSLNLHLYSSPSLPHTHPYPMTNIPSFAQWPYDCIEEDELEQDSGSQPSMTTESSETSSQCTSSDSVTGLSTLTIAVHPSITMDSFNNNESSSFLCSSTPSSTMASPLMFQKEEDLQPNGTNFITANKTSHIAVGLSTVV

Target ORF information:

RefSeq Version	XM_011605705.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (plekhg4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011605705.1

ORF Insert Sequence:

ATGTACGGCA GCGTCTTCAC AGCTGAGTTT CTGCAGAATG TAACTCGGGA CCGTAACCTG 
CACCCGCTCC AGAACTGCCT GCTCACCACC GGCACAGCCG TGTACAGGAC GCCGTGGAAG 
AACGTGGTCA ACCCACTGTT TGTCAGCAGC ACGTCAGACA CCATCATGCA AGCGCGCTGC 
AGCAGAGGCG CCTTCCGTGG TCATCTCAGC ACCTGCAGCA CCAGCGGATC CACCGGGACC 
CTGGACAGCC ACCGCAGCTC GATAGAGTCG CTGCACTCTC AGGGGACTGA TTCTATCTTC 
TCTGAGCCCA CCTCCCCAAA CAGAAACCCC ATGGACCCAA ATGGTGAGGT CCATGGTACC 
AGTGATACTG CAGTACCTAT AATTAAACTT GAAAGGTCTG GTGAAGAGTG TGAGACAGGT 
CAGGAGTCCG AAGACAATGA TGGGAGAGAG GGGGGGCCTG GGTCCAAGAT GCTCTCTTTC 
ACCACAGACC TTAGCAACCC AGGATGTCGT CGCCGTCCCC CAAGGGACTC TGTAGCATTT 
GAGAGCAGGA GACTTTTCAG GAAGTCTTAC ATGGAAGCCC TGCAGAACCC CATGAGCCTT 
GGGTCCAGCT CTGAATCCAT TCTGGAGGAA CAGTCTGAGC ACAGCATTGC CCTGGGAGAT 
AGAGCAGTGA CACCTGGCAG CAGCCCTGAG GCCAGAACTC CTTCTAGAGA ACTCTTGTCG 
CGAAGGCTGG TTGGTCGGGG GTGGCTCAGC AGCGATGAAT CCAGGCCCAG CACACCTTTG 
CTGTATTTAC AAAGGGGGTT GAGGAGCGCT GAGAGGCGGG CGGAGAGACG TTCAAAGTCA 
CTGGAGAGAA CAAACAAAGG CGGCCACGTT AAAGGCCACC GGGAAAGATC ATCCTCCGGA 
GGCACCGTCA ACATTTCCCC GAAAAAACTG ATGAACGGCT ATGCTCTGCG TTTCGGAAAG 
CTAGACGTCG AGGCGGCACT TCCTGGGTCG GAGAGAAGGA CCAACAAGGA GGAGTCAGGC 
CAGCGTGGCG ACGGCGTCAG CGGCGAGGGC AGGCGACACA GACTCATCAG CGAGGATTCA 
CACTCTCCTA AAAGTGCCAA TGGATCAGCC ATCACGCTGG CGCCCGTAAC AAGGCCTTCA 
TGCGAGAGCA GCTCAGCTCT CCCCAAACTG GTGTCAGAGG TCAATCAGGA GCTCTTCACA 
TCGGGGGCCG TGATCCTGCC AGGAAGCAGA GATCGTGGTG GGAGGGCGGT GCTGCAGGTG 
TGCGCCAGAG CTCAGGTGTG GGCCGGGGAG AGTTGCACGG TCAATGACCT CACCTGTTTA 
CTTGGTTACT ACTACTCCAC ACTGCGGAAG GAAAGGCGAG ATCAAGGCCT GTCTGTTGTA 
ATAGACGGCA GGAGGCAGCA GCCTGTTCCA GCTCTGTTGT TGTCTCTGTC TGAGTTGCAG 
GCATTGGTAC CAAATGCACT TTATTCGGTT CTCTTCCTGG TGGACAAAGA GACAGTGGCC 
AAACCAGAGA GAGACATGAA TGTGCAGACT GAAACGTTGA CATCTCTAAA AGCCTTGCTG 
AAGCACATTG ACCAAAACCA GCTCACGCGA GACCTGGACG GCACCTTCCA CTACGACCAC 
AGCCACTGGA TCCACTTCAG GCAGAAAATC GAACCCTTCG CCAGCAGCTG TTCTGCAGCT 
ATCTCCTCCC TGCAGCAGTC GATCGACTCA CTGGGCAACA GCGGCAACTT AAAGACGTCA 
AAGGAAGTGT CTGAGGTGCT GGAGCAGCAG CGACACCTCA TGAAGAAGGT ACTGGACGAC 
ACGCAGCTAA ACCGGCTGCG TCTAGAAGGA GGCACCGTCC TGGCCCGCAT CAGGAAAGAA 
GAAGCCTGTG AGAATGAAAA CTACAAAGAT GCCATTGACA TGTTAAGTGC GCTGTACAAC 
CAAGTAGACG AAGAAGTCCA CAAACTTGTG ATTCTGTCCA ACAAGTGTCA GAAGGAGCTG 
GAGAGCCTGC TGGAGGTGCG CATGTTTGAA GAGCAAACAC AGCAGATCAA AGTCTGGTTC 
AGCAGCGAGG GAGAGAAGCA GCTCGTACCT CTGGAATCAC AAACTCTGTC GGTGGCCAAA 
ATCAAGGAGA TGAGAGCCAG TTTGGAGCAC TTTCTGGAGG AGTCCGTGCA CCAGCAGAGA 
CATGGTCTGC AGCTGGTGAA GGAGTCCCCA GAGTCCCTTC CAGGCTCTGC CCTGATGGAC 
TTTAAACAAC ATCTGGGCTC CATTCTGAGC CGAGTAGAGA AAAGGAAGGA CCACTTAGAA 
ATCCTTACAA ATCTCTATGA ATTCTATGAC TCAGTGAACA AGTGGATGGA GCACTGCCAG 
GATTATTTCC ATCAGCTGAG TCTGGACAGC ACAAGCTTGA TTCTGTCGCC CTCTGTGGAG 
CAGACCCTCC AGGACTACTA CGCAGAAGCG TCCAAGTTTT CCATGGATAA CTTCAGCACC 
CTCAACAACA TGGTGCTCTC CCTGGAAAGC CCTCAGCAGC TGCAGCACTG GAACTCTGTG 
TGGCATAAAT GCCAACAAAC CAAACAGCAG CTGGAAGAAA TTCTGGCCCG AGCCACGACG 
GCCCAGGACC CCACGGCCAC CGACGTAAAG ACCCCAGAGA GTGGCCCCAG CAAACCAGAG 
CAGCACGGAG CGAACGCGTG CGTGCTGCTA CCTTTTGAGG ACAGCAAGCC TCATTCTTCT 
GAGCCTTACT CCAAGAGCCC CACCAGCTCC ATCTCCTCCT CCTCGCACTT CATTTTCCCC 
TCTGACTGTG ACAGCAAACT GAGGCAAAGT CCGTCCATGT TTGAGGACAC GGACAGCGAC 
TGCACCGTGG ACTCGTCCGT CTCCTGCCAC TCGGAGCCCA TCTACACCGG CACCGCCCGC 
CTCCGCAAGC ACCCCATGAA GAAGATCATG AAGAAGACCG TGAGCTATGA GCTGAACGCA 
AGGGACAGCG TCCACTCGGA TGCCGGCCAC ATTCACGGCT ACACGGGCGT GTACATCAAG 
GGTTTGGAGG TGACGAACAA CGTGTCCGCG GAGAAGAAGT TGCAGAGGCC CGACGTGACG 
AGCCCGGCTT TGGGACGCAG CCGTAGCATG TCCACACCGT CCAGGGCGCA CAGCAGACAC 
AGTGACGGAG ATATTAAGAA ACAGAGCAGC AGCAAAGTGC AGCACATCAT GGACGAGATG 
ATTTCCACGG AGCGGGAGTA CGTCCGCTCC CTCAGCTACA TCATCGAGCA GTATTTCCCA 
GAGATGGAGC GGCTGGATTT GCCCCAGGAC CTGCGAGGGA AGCGCAGCAT CATTTTTGGA 
AACGTGGAGA AGCTGTGGGA CTTTCACACT CAGTACTTCC TGAAGGATCT GGAGTCGTGT 
GCTCACTGTC CACTCTCGAT CAGCAGCTGT TTTCTCAGAC ATGAGGATCA GTTTGGAATG 
TATGCCCTCT ACAGCAAGAA TAAGCCGCAG TCTGACGCTC TGCTCAGCAG CCACGGGAAT 
GAATTCTTCA AGAACAAACA GATCGAGCTG GGCGACAAGA TGGACTTAGC GTCCTACTTG 
CTGAAGCCCA TCCAGAGGAT GAGTAAATAT GCTCTGCTGC TCAAAGACCT GATCAAGGAG 
TGCAGTCAGT CTCAGGAGCA GGAGCTGAGT GACCTCCGCA CGGCAGAGGA CATGGTCAAA 
TTTCAGCTTC GCCATGGCAA TGACCTGCTG GCTATGGATG CTATTCGTGG ATGTGATGTG 
AACCTGAAAG AGCAGGGTCA GCTCTGCTGC CAGGACGAGT TCATCGTCTG GTGCGGGAGG 
AGGAAGTACC TCCGCCATGT CTTCTTGTTT GAAGACCTCA TCCTGTTCAG CAAGACCAAA 
AAGATCGAAG GAGGATATGA CATTTACATC TACAAACAGT CTTTCAAGAC GGCAGAGATC 
GGTATGACGG AGAACGTCGG GGACAGCGGC CTACGCTTCG AGATTTGGTT CCGCAGGAGG 
AAGTCACAGG ACACCTTCAT ACTCCAAGCC AGCAGTGCGG AGGTCAAGGC CGTGTGGACA 
GCCATCATTG GGAAGATCCT GTGGAGACAG GCGCTCAGAA ATCGAGCCTT GCGCATGCAG 
GAGATGGTGT CCATGGGAAT TGGAAGTAAG CCCTTCATGG ACATTAAGCC AAGCGATGCG 
GCCATCAGCG ACAGGGCCAT TGATTATATC ATGAAGGGGA CAGAAGCCAG GACCAGGGCG 
TCCATTGCTG TGCCTTCCTT TGAACACGCC ACTTCCTTCA AGCGACCCCA CTCCACCATC 
TCCAACAGCA GCACCTCCTC CTCCAGCAGC CAGTCCTCCT CCTCGTTGCT GGGCTCGCTC 
AACCTCCATC TTTACTCCTC CCCCTCTCTC CCACACACAC ATCCGTACCC GATGACCAAC 
ATCCCCTCCT TTGCTCAGTG GCCGTACGAC TGCATCGAGG AGGATGAGCT GGAGCAGGAC 
AGTGGGAGTC AACCCTCTAT GACCACTGAG AGCTCGGAGA CCTCGTCGCA GTGCACCTCC 
AGTGACAGCG TGACTGGGCT GAGCACGCTG ACGATAGCTG TGCATCCCAG CATCACCATG 
GACTCTTTCA ACAACAACGA ATCCTCGTCT TTCCTGTGCT CCTCCACACC CTCCTCCACC 
ATGGCTTCTC CTTTAATGTT CCAAAAGGAG GAAGACCTCC AGCCCAATGG CACCAACTTC 
ATCACAGCAA ACAAGACCTC TCATATAGCA GTAGGCCTCT CTACTGTGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

plekhg4b cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム