atp8b4 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp8b4
Entrez Gene ID	101074349
Full Name	ATPase phospholipid transporting 8B4 (putative)
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011607650.2	XP_011605952.2	probable phospholipid-transporting ATPase IM
XM_011607650.1	XP_011605952.1	probable phospholipid-transporting ATPase IM

Pathways from BioSystems
Gene Ontology

The following atp8b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp8b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo57400	XM_011607650.2 Latest version!	Takifugu rubripes ATPase phospholipid transporting 8B4 (putative) (atp8b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo28243	XM_011607650.1 Latest version!	Takifugu rubripes ATPase, class I, type 8B, member 4 (atp8b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp8b4 ( XM_011607650.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605952.2 Takifugu rubripes ATPase phospholipid transporting 8B4 (putative) (atp8b4), mRNA.

Price & Availability↑

CloneID	OTo57400
Clone ID Related Accession (Same CDS sequence)	XM_011607650.2
Accession Version	XM_011607650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	probable phospholipid-transporting ATPase IM
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCT GGAGGAGAAA CGCCACCGTC AAGAAGGAAC GGCGTGTGAA AGCGAACGCC 
CGGGACCACA ACAGAAACTT CTCCTATGCT GACAATCACA TCAAAACGTC CAAGTACAAC 
GTCTTCACCT TCCTGCCGCT CAATCTGTTC GAGCAGTTCC AGAGAGTCGC CAACGCTTAC 
TTCTTAGTGC TGCTGATACT GCAGTTGATC CCTCAAATTT CATCCCTCTC CTGGTTCACC 
ACCATCGTGC CTTTGGTGCT GGTGCTGGTC ACCTCGGCCG TCAAGGACGC CAGAGACGAC 
TACTTCCGAC ACAAGAGTGA TCGGCAAGTC AACAACCGCC AGTCGCAGGT CATCATCGGG 
GGACGCTTGC AGAACGAGAA GTGGAAGAAC ATTCAAGTGG GCGACGTCAT CAAGCTGGAG 
AATAATCAGA GTGTTGCCGC GGACGTCCTC CTTCTCTGCA GCAGCGAACC CTGCGGACTC 
TGTTACATCG AGACCGCGGC GCTCGATGGA GAGACCAATC TGAAAGTCCG CCACGCGCTG 
AGCGTCACCT CGGAGATGGG AGACGTTGCA GCCTTGATGG CCTTTGATGG AGAAGTCATC 
TGTGAAACTC CCAACAACAA GCTGGATAAA TTCACAGGAA CGTTGTGCTG GAGAGGGAGC 
AGATACCCTC TGGATATTGG AAAAATGCTG CTGCGAGGGT GCGTACTCAG GAACACGGAG 
TGGTGCTTTG GGATGGTTGT CTTTGCTGGA CAGCAAACCA AGCTAATGCA GAACTGTGGA 
AAGGCCACGT TTAAGAGGAC AAGTATCGAC AAACTGATGA ACACTCTGGT TTTACTGATC 
TTTGCCTTCC TTATCTTTAT GGGAGCTATT TTGGCCATTG GAAACACCAT TTGGGAGAGC 
TTGGTCGGAG TAAACTTCCA GGATTATTTG CCCTGGGACA CCGTTCAGAG GAACGCTGTT 
TTCTCCGGCT TCCTCACCTT CTGGTCCTAC ATCATCATCC TCAACACGGT GGTACCCATC 
TCTCTGTATG TCAGTATGGA GATTCTGAGG CTCGGCCACA GTTACTTCAT CAACTGGGAC 
CGGAGGATGT ACCATGCCAA GACGGACACT CCGGCAGAAG CTCGCACCAC CACCCTGAAC 
GAGGAGCTGG GCCAGGTGGA CTTCATCTTT ACGGACAAGA CGGGCACCCT CACGCAGAAC 
ATCATGGTCT TCAGAAAGTG CTCCATCAAT GGAAAAACAT ATGGGGATGT TTTTGATGAG 
TTCAACCAGA AGGTGGAAAT TACAGAGAAA ACTGTCGGCG TGGACTTCTC CTTCAACCCT 
CTCCGTGACC CAAGGTTTCA GTTTTATGAT AACAGCCTGC TGGAGGCCAT CGAGCTAGAG 
GAACCCGCCG TGCAGGAGTT CTTCAGGCTG CTGGCGGTGT GTCACACTGT CATGGCGGAG 
GAGAAGACTG AAGGGCGTTT GCTGTATCAG GCTCAGTCAC CCGACGAGGG AGCTCTGGTC 
ACAGCAGCAC GCAACTTTGG GTTCGTCTTC CGCTCCCGGA CCCCCGAGAC CATCAGCGTG 
TGTGAGATGG GACGAGCCGT GACCTACCAG CTGCTGGCCA TACTGGACTT CGACAACGTG 
CGAAAGAGAA TGAGCGTCAT TGTGAGAAAC CCAGAGGGTG ACATTAAACT CTACTCCAAA 
GGGGCTGACA CCATCATGTT TGACCTTCTG GATCCTTCCT GTGAAAACCT CATGCATGTC 
ACCTCAGACC ACCTCGGCGA ATTTGCAGCA GATGGGCTTC GCACGTTAGC TCTGGCTTAC 
AAGGACCTGG ATGAAGAGGA TTTTGGAGTC TGGATGCAGA AGCTTCACTC TGCCAGCACT 
GTCATCGAGG ACCGTGAGGC TCAGCTGGCG GTTACGTACG AGGAGATCGA ACGCGGCTTG 
AAGCTTCTAG GCGCCACAGC AATCGAGGAC AAACTTCAGG AAGGAGTCCC AGAAACAATC 
GCCAGCCTCC ATCTCACTGA CATTAAGATC TGGATCCTCA CTGGGGACAA ACTCGAGACG 
GCCGTGAACA TCGGCTACTC CTGCAGCATG CTGCGAGACG ACATGGAGGA GGTGTTTGTC 
GTTTCCGGTG CGACGTCACA AGACGTGCAG CATCAGCTCA GGGAGGCTAA AGGTCAAATT 
TTGGCCACAA GTCGAGCAAG CTGGAGAGAA GATGGTGGGG GGCCAGACGC GGCTGCAGAT 
CAGCCTCTTT CCAAAGAAGC CGTTACAGAA GAGTTCGCGT TAGTCATCAA CGGACACAGT 
CTGGCTCACG CTCTGGAGCC ACGGCTGGAG CTGCTGTTCC TGGACGTGGC CTGCCTGTGC 
AAATCCATCA TCTGCAGCCG GGTGACACCG CTGCAGAAGG CCCAGGTGGT GGAGCTGGTG 
AAGAGGTGCA AGAGGGCCGT CACGCTGGCC ATTGGAGACG GCGCCAATGA CGTCAGCATG 
ATCCAGACTG CTCACATCGG CATCGGCATC AGCGGTCAGG AGGGGATGCA GGCGGTTCTG 
GCCTCAGATT ACTCCTTCGC TCAGTTTCGG TACCTGCAGA GGCTCCTGCT GGTACACGGA 
CGCTGGTCCT ACTTCCGCAT TTGCCACTTC CTGTACTACT TCTTCTACAA GAACTTTGCC 
TTCACTCTGG TCCACTTCTG GTACAGCTTC TTCTGTGGTT TCTCAGCTCA GACTGTCTAT 
GACCAGTGGT TTATCACCCA CTTCAATGTC ATCTATACAT CTTTACCAGT TATGGGGATG 
GGACTTTTTG ATCAGGATGT CAGCGACCAG CACAGCCTTC GCTACCCAAG CTTGTACAAG 
CCGGGCCAGC AGAACCTTCT CTTCAACAGA CGGCAGTTCT TCCTGTGCGC GCTGCAGGGC 
CTGACAACAT CCTGCCTCCT ATTCTTCATT CCCTATGGAG CCTTCGCTGC CATGGAGAAG 
GAGGACGGCA CCCAGATTTC CGATCAGCAA ACATTTGCCG TCACCGTGGC AACATCACTG 
ATCCTTGTCG TGAGTGTGGA GATTGGACTG GAGAAGCACT ACTGGACAGC CATCAATCAG 
CTGTTCCTAG GGGGAAGTCT CACCATGTAC TTCGCCATTT TGTTCACGAT GCACAGCGAC 
GGCATGTTTG CAGTGAATCA GAACCATTTT CCATTTGTAG GGACATCACG CAACTGTCTG 
TCAGAGGCCA ACGTGTGGCT GGTGGTTGTG CTCACCACTG TGGTGTGTGT CGTGCCCAGT 
TTAACACTCA GGTTCCTGAG GGTTGACCTG TCCCCCACCA CAACAGATAA GGTTCGTCAT 
CTGCAACTAT CCAGAAAGAA GGAAAAACCG CAGGAGCACA TTCCGAAGCC GGTACGCAGG 
ACCAGTTTTC GCCGTTCTGC CTACGCCTTC TCCCACCAGC AGGGCTACGG GGAGCTGATT 
ACCTCGGGCA AAAACATGCG GACAAGCTCA GTCTCGTCCG TACGTTCCCC TCAGTCGCCA 
CCACACTCCA GCCTCAAATG GATGGAAAAC ATACTGAAGG AAAAGAACAA AGTGGCGTGT 
GTCTCTGGTG AAAATGTCAT GAACAACGGG GAAGTCGACA GGGAACAAGA CGCCAAAACA 
GTTGACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605952.2
CDS	721..4332
Translation	MDFWRRNATVKKERRVKANARDHNRNFSYADNHIKTSKYNVFTFLPLNLFEQFQRVANAYFLVLLILQLIPQISSLSWFTTIVPLVLVLVTSAVKDARDDYFRHKSDRQVNNRQSQVIIGGRLQNEKWKNIQVGDVIKLENNQSVAADVLLLCSSEPCGLCYIETAALDGETNLKVRHALSVTSEMGDVAALMAFDGEVICETPNNKLDKFTGTLCWRGSRYPLDIGKMLLRGCVLRNTEWCFGMVVFAGQQTKLMQNCGKATFKRTSIDKLMNTLVLLIFAFLIFMGAILAIGNTIWESLVGVNFQDYLPWDTVQRNAVFSGFLTFWSYIIILNTVVPISLYVSMEILRLGHSYFINWDRRMYHAKTDTPAEARTTTLNEELGQVDFIFTDKTGTLTQNIMVFRKCSINGKTYGDVFDEFNQKVEITEKTVGVDFSFNPLRDPRFQFYDNSLLEAIELEEPAVQEFFRLLAVCHTVMAEEKTEGRLLYQAQSPDEGALVTAARNFGFVFRSRTPETISVCEMGRAVTYQLLAILDFDNVRKRMSVIVRNPEGDIKLYSKGADTIMFDLLDPSCENLMHVTSDHLGEFAADGLRTLALAYKDLDEEDFGVWMQKLHSASTVIEDREAQLAVTYEEIERGLKLLGATAIEDKLQEGVPETIASLHLTDIKIWILTGDKLETAVNIGYSCSMLRDDMEEVFVVSGATSQDVQHQLREAKGQILATSRASWREDGGGPDAAADQPLSKEAVTEEFALVINGHSLAHALEPRLELLFLDVACLCKSIICSRVTPLQKAQVVELVKRCKRAVTLAIGDGANDVSMIQTAHIGIGISGQEGMQAVLASDYSFAQFRYLQRLLLVHGRWSYFRICHFLYYFFYKNFAFTLVHFWYSFFCGFSAQTVYDQWFITHFNVIYTSLPVMGMGLFDQDVSDQHSLRYPSLYKPGQQNLLFNRRQFFLCALQGLTTSCLLFFIPYGAFAAMEKEDGTQISDQQTFAVTVATSLILVVSVEIGLEKHYWTAINQLFLGGSLTMYFAILFTMHSDGMFAVNQNHFPFVGTSRNCLSEANVWLVVVLTTVVCVVPSLTLRFLRVDLSPTTTDKVRHLQLSRKKEKPQEHIPKPVRRTSFRRSAYAFSHQQGYGELITSGKNMRTSSVSSVRSPQSPPHSSLKWMENILKEKNKVACVSGENVMNNGEVDREQDAKTVDT

Target ORF information:

RefSeq Version	XM_011607650.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes ATPase phospholipid transporting 8B4 (putative) (atp8b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607650.2

ORF Insert Sequence:

ATGGACTTCT GGAGGAGAAA CGCCACCGTC AAGAAGGAAC GGCGTGTGAA AGCGAACGCC 
CGGGACCACA ACAGAAACTT CTCCTATGCT GACAATCACA TCAAAACGTC CAAGTACAAC 
GTCTTCACCT TCCTGCCGCT CAATCTGTTC GAGCAGTTCC AGAGAGTCGC CAACGCTTAC 
TTCTTAGTGC TGCTGATACT GCAGTTGATC CCTCAAATTT CATCCCTCTC CTGGTTCACC 
ACCATCGTGC CTTTGGTGCT GGTGCTGGTC ACCTCGGCCG TCAAGGACGC CAGAGACGAC 
TACTTCCGAC ACAAGAGTGA TCGGCAAGTC AACAACCGCC AGTCGCAGGT CATCATCGGG 
GGACGCTTGC AGAACGAGAA GTGGAAGAAC ATTCAAGTGG GCGACGTCAT CAAGCTGGAG 
AATAATCAGA GTGTTGCCGC GGACGTCCTC CTTCTCTGCA GCAGCGAACC CTGCGGACTC 
TGTTACATCG AGACCGCGGC GCTCGATGGA GAGACCAATC TGAAAGTCCG CCACGCGCTG 
AGCGTCACCT CGGAGATGGG AGACGTTGCA GCCTTGATGG CCTTTGATGG AGAAGTCATC 
TGTGAAACTC CCAACAACAA GCTGGATAAA TTCACAGGAA CGTTGTGCTG GAGAGGGAGC 
AGATACCCTC TGGATATTGG AAAAATGCTG CTGCGAGGGT GCGTACTCAG GAACACGGAG 
TGGTGCTTTG GGATGGTTGT CTTTGCTGGA CAGCAAACCA AGCTAATGCA GAACTGTGGA 
AAGGCCACGT TTAAGAGGAC AAGTATCGAC AAACTGATGA ACACTCTGGT TTTACTGATC 
TTTGCCTTCC TTATCTTTAT GGGAGCTATT TTGGCCATTG GAAACACCAT TTGGGAGAGC 
TTGGTCGGAG TAAACTTCCA GGATTATTTG CCCTGGGACA CCGTTCAGAG GAACGCTGTT 
TTCTCCGGCT TCCTCACCTT CTGGTCCTAC ATCATCATCC TCAACACGGT GGTACCCATC 
TCTCTGTATG TCAGTATGGA GATTCTGAGG CTCGGCCACA GTTACTTCAT CAACTGGGAC 
CGGAGGATGT ACCATGCCAA GACGGACACT CCGGCAGAAG CTCGCACCAC CACCCTGAAC 
GAGGAGCTGG GCCAGGTGGA CTTCATCTTT ACGGACAAGA CGGGCACCCT CACGCAGAAC 
ATCATGGTCT TCAGAAAGTG CTCCATCAAT GGAAAAACAT ATGGGGATGT TTTTGATGAG 
TTCAACCAGA AGGTGGAAAT TACAGAGAAA ACTGTCGGCG TGGACTTCTC CTTCAACCCT 
CTCCGTGACC CAAGGTTTCA GTTTTATGAT AACAGCCTGC TGGAGGCCAT CGAGCTAGAG 
GAACCCGCCG TGCAGGAGTT CTTCAGGCTG CTGGCGGTGT GTCACACTGT CATGGCGGAG 
GAGAAGACTG AAGGGCGTTT GCTGTATCAG GCTCAGTCAC CCGACGAGGG AGCTCTGGTC 
ACAGCAGCAC GCAACTTTGG GTTCGTCTTC CGCTCCCGGA CCCCCGAGAC CATCAGCGTG 
TGTGAGATGG GACGAGCCGT GACCTACCAG CTGCTGGCCA TACTGGACTT CGACAACGTG 
CGAAAGAGAA TGAGCGTCAT TGTGAGAAAC CCAGAGGGTG ACATTAAACT CTACTCCAAA 
GGGGCTGACA CCATCATGTT TGACCTTCTG GATCCTTCCT GTGAAAACCT CATGCATGTC 
ACCTCAGACC ACCTCGGCGA ATTTGCAGCA GATGGGCTTC GCACGTTAGC TCTGGCTTAC 
AAGGACCTGG ATGAAGAGGA TTTTGGAGTC TGGATGCAGA AGCTTCACTC TGCCAGCACT 
GTCATCGAGG ACCGTGAGGC TCAGCTGGCG GTTACGTACG AGGAGATCGA ACGCGGCTTG 
AAGCTTCTAG GCGCCACAGC AATCGAGGAC AAACTTCAGG AAGGAGTCCC AGAAACAATC 
GCCAGCCTCC ATCTCACTGA CATTAAGATC TGGATCCTCA CTGGGGACAA ACTCGAGACG 
GCCGTGAACA TCGGCTACTC CTGCAGCATG CTGCGAGACG ACATGGAGGA GGTGTTTGTC 
GTTTCCGGTG CGACGTCACA AGACGTGCAG CATCAGCTCA GGGAGGCTAA AGGTCAAATT 
TTGGCCACAA GTCGAGCAAG CTGGAGAGAA GATGGTGGGG GGCCAGACGC GGCTGCAGAT 
CAGCCTCTTT CCAAAGAAGC CGTTACAGAA GAGTTCGCGT TAGTCATCAA CGGACACAGT 
CTGGCTCACG CTCTGGAGCC ACGGCTGGAG CTGCTGTTCC TGGACGTGGC CTGCCTGTGC 
AAATCCATCA TCTGCAGCCG GGTGACACCG CTGCAGAAGG CCCAGGTGGT GGAGCTGGTG 
AAGAGGTGCA AGAGGGCCGT CACGCTGGCC ATTGGAGACG GCGCCAATGA CGTCAGCATG 
ATCCAGACTG CTCACATCGG CATCGGCATC AGCGGTCAGG AGGGGATGCA GGCGGTTCTG 
GCCTCAGATT ACTCCTTCGC TCAGTTTCGG TACCTGCAGA GGCTCCTGCT GGTACACGGA 
CGCTGGTCCT ACTTCCGCAT TTGCCACTTC CTGTACTACT TCTTCTACAA GAACTTTGCC 
TTCACTCTGG TCCACTTCTG GTACAGCTTC TTCTGTGGTT TCTCAGCTCA GACTGTCTAT 
GACCAGTGGT TTATCACCCA CTTCAATGTC ATCTATACAT CTTTACCAGT TATGGGGATG 
GGACTTTTTG ATCAGGATGT CAGCGACCAG CACAGCCTTC GCTACCCAAG CTTGTACAAG 
CCGGGCCAGC AGAACCTTCT CTTCAACAGA CGGCAGTTCT TCCTGTGCGC GCTGCAGGGC 
CTGACAACAT CCTGCCTCCT ATTCTTCATT CCCTATGGAG CCTTCGCTGC CATGGAGAAG 
GAGGACGGCA CCCAGATTTC CGATCAGCAA ACATTTGCCG TCACCGTGGC AACATCACTG 
ATCCTTGTCG TGAGTGTGGA GATTGGACTG GAGAAGCACT ACTGGACAGC CATCAATCAG 
CTGTTCCTAG GGGGAAGTCT CACCATGTAC TTCGCCATTT TGTTCACGAT GCACAGCGAC 
GGCATGTTTG CAGTGAATCA GAACCATTTT CCATTTGTAG GGACATCACG CAACTGTCTG 
TCAGAGGCCA ACGTGTGGCT GGTGGTTGTG CTCACCACTG TGGTGTGTGT CGTGCCCAGT 
TTAACACTCA GGTTCCTGAG GGTTGACCTG TCCCCCACCA CAACAGATAA GGTTCGTCAT 
CTGCAACTAT CCAGAAAGAA GGAAAAACCG CAGGAGCACA TTCCGAAGCC GGTACGCAGG 
ACCAGTTTTC GCCGTTCTGC CTACGCCTTC TCCCACCAGC AGGGCTACGG GGAGCTGATT 
ACCTCGGGCA AAAACATGCG GACAAGCTCA GTCTCGTCCG TACGTTCCCC TCAGTCGCCA 
CCACACTCCA GCCTCAAATG GATGGAAAAC ATACTGAAGG AAAAGAACAA AGTGGCGTGT 
GTCTCTGGTG AAAATGTCAT GAACAACGGG GAAGTCGACA GGGAACAAGA CGCCAAAACA 
GTTGACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp8b4 ( XM_011607650.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605952.1 Takifugu rubripes ATPase, class I, type 8B, member 4 (atp8b4), mRNA.

Price & Availability↑

CloneID	OTo28243
Clone ID Related Accession (Same CDS sequence)	XM_011607650.1
Accession Version	XM_011607650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	probable phospholipid-transporting ATPase IM
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCT GGAGGAGAAA CGCCACCGTC GAGAAGGAAC GGCGTGTGAA AGCGAACGCC 
CGGGACCACA ACAGAAACTT CTCCTATGCT GACAATCACA TCAAAACGTC CAAGTACAAC 
GTCTTCACCT TCCTGCCGCT CAATCTGTTC GAGCAGTTCC AGAGAGTCGC CAACGCTTAC 
TTCTTAGTGC TGCTGATACT GCAGTTGATC CCTCAAATTT CATCCCTCTC CTGGTTCACC 
ACCATCGTGC CTTTGGTGCT GGTGCTGGTC ACCTCGGCCG TCAAGGACGC CAGAGACGAC 
TACTTCCGAC ACAAGAGTGA TCGGCAAGTC AACAACCGCC AGTCGCAGGT CATCATCGGG 
GGACGCTTGC AGAATGAGAA GTGGAAGAAT ATTCAAGTGG GCGACGTCAT CAAGCTGGAG 
AATAATCAGA GTGTTGCCGC GGACGTCCTC CTTCTCTGCA GCAGCGAACC CTGCGGACTC 
TGTTACATCG AGACCGCGGC GCTCGATGGA GAGACCAATC TGAAAGTCCG CCACGCGCTG 
AGCGTCACCT CGGAGATGGG AGACGTTGCA GCCTTGATGG CCTTTGATGG AGAAGTCATC 
TGTGAAACTC CCAACAACAA GCTGGATAAA TTCACAGGAA CGTTGTGCTG GAGAGGGAGC 
AGATACCCTC TGGATATTGG CAAAATGCTG CTGCGAGGGT GCGTACTCAG GAACACGGAG 
TGGTGCTTTG GGATGGTTGT CTTTGCTGGA CAGCAAACCA AGCTAATGCA GAACTGTGGA 
AAGGCCACGT TTAAGAGGAC AAGTATCGAC AAACTGATGA ACACTCTGGT TTTACTGATC 
TTTGCCTTCC TTATCTTTAT GGGAGCTATT TTGGCCATTG GAAACACCAT TTGGGAGAGC 
TTGGTCGGAG TAAACTTCCA GGATTATTTG CCCTGGGACA CCGTTCAGAG GAACGCTGTT 
TTCTCCGGCT TCCTCACCTT CTGGTCCTAC ATCATCATCC TCAACACGGT GGTACCCATC 
TCTCTGTATG TCAGTATGGA GATTCTGAGG CTCGGCCACA GTTACTTCAT CAACTGGGAC 
CGGAGGATGT ACCATGCCAA GACGGACACT CCGGCAGAAG CTCGCACCAC CACCCTGAAC 
GAGGAGCTGG GCCAGGTGGA CTTCATCTTT ACGGACAAGA CGGGCACCCT CACGCAGAAC 
ATCATGGTCT TCAGAAAGTG CTCCATCAAT GGAAAAACAT ATGGGGATGT TTTTGATGAG 
TTCAACCAGA AGGTGGAAAT TACAGAGAAA ACTGTCGGCG TGGACTTCTC CTTCAACCCT 
CTCCGTGACC CAAGGTTTCA GTTTTATGAT AACAGCCTGC TGGAGGCCAT CGAGCTAGAG 
GAACCCGCCG TGCAGGAGTT CTTCAGGCTG CTGGCGGTGT GTCACACTGT CATGGCGGAG 
GAGAAGACTG AAGGGCGTTT GCTGTATCAG GCTCAGTCAC CCGACGAGGG AGCTCTGGTC 
ACAGCAGCAC GCAACTTCGG GTTCGTCTTC CGCTCCCGGA CCCCCGAGAC CATCAGCGTG 
TGTGAGATGG GACGAGCCGT GACCTACCAG CTGCTGGCCA TACTGGACTT CGACAACGTG 
CGGAAGAGAA TGAGCGTCAT TGTGAGAAAC CCAGAGGGTG ACATTAAACT CTACTCCAAA 
GGGGCTGACA CCATCATGTT TGACCTTCTG GATCCTTCCT GTGAAAACCT CATGCATGTC 
ACCTCAGACC ACCTCGGCGA ATTTGCAGCA GATGGGCTTC GCACGTTAGC TCTGGCTTAC 
AAGGACCTGG ATGAAGAGGA TTTTGGAGTC TGGATGCAGA AGCTTCACTC TGCCAGCACT 
GTCATCGAGG ACCGCGAGGC TCAGCTGGCG GTTACGTACG AGGAGATCGA ACGCGGCTTG 
AAGCTTCTAG GCGCCACAGC AATCGAGGAC AAACTTCAGG AAGGAGTCCC AGAAACAATC 
GCCAGCCTCC ATCTCGCTGA CATTAAGATC TGGATCCTCA CTGGGGACAA ACTCGAGACG 
GCCGTGAACA TCGGCTACTC CTGCAGCATG CTGCGAGACG ACATGGAGGA GGTGTTTGTC 
GTTTCCGGTG CGACGTCACA GGACGTGCAG CATCAGCTCA GGGAGGCTAA AGGTCAAATT 
TTGGCCACAA GTCGAGCAAG CTGGAGAGAA GATGGTGGGG GGCCAGACGC GGCTGCAGAT 
CAGCCTCTTT ACAAAGAAGC CGTTACAGAA GAGTTTGCGT TAGTCATCAA CGGACACAGT 
CTGGCTCACG CTCTGGAGCC ACGGCTGGAG CTGCTGTTCC TGGACGTGGC CTGCCTGTGC 
AAATCCATCA TCTGCAGCCG GGTGACGCCG CTGCAGAAGG CCCAGGTGGT GGAGCTGGTG 
AAGAGGTGCA AGAGGGCCGT CACGCTGGCC ATTGGAGACG GCGCCAATGA CGTCAGCATG 
ATCCAGACTG CTCACATCGG CATCGGCATC AGCGGTCAGG AGGGGATGCA GGCGGTTCTG 
GCCTCAGATT ACTCCTTCGC TCAGTTTCGG TACCTGCAGA GGCTCCTGCT GGTACACGGA 
CGCTGGTCCT ACTTCCGCGT TTGCCACTTC CTGTACTACT TCTTCTACAA GAACTTTGCC 
TTCACTCTGG TCCACTTCTG GTACAGCTTC TTCTGTGGTT TCTCAGCTCA GACTGTCTAT 
GACCAGTGGT TTATCACCCA CTTCAATGTC ATCTATACAT CTTTACCAGT TATGGGGATG 
GGACTTTTTG ATCAGGATGT CAGCGACCAG CACAGCCTTC GCTACCCAAG CTTGTACAAG 
CCGGGCCAGC AGAACCTTCT CTTCAACAGA CGGCAGTTCT TCCTGTGCGC GCTGCACGGC 
CTGACAACAT CCTGCCTCCT ATTCTTCATT CCCTATGGAG CCTTCGCTGC CATGGAGAAG 
GAGGACGGCA CCCAGATTTC CGATCAGCAA ACATTTGCCG TCACCGTGGC AACATCACTG 
ATCCTTGTCG TGAGTGTGGA GATTGGACTG GAGAAGCACT ACTGGACAGC CATCAATCAG 
CTGTTCCTGG GGGGAAGTCT CACCATGTAC TTCGCCATTT TGTTCACGAT GCACAGCGAC 
GGCATGTTTG CAGTGAATCA GAGCCATTTT CCATTTGTAG GGACATCACG CAACTGTCTG 
TCAGAGGCCA ACGTGTGGCT GGTGGTTGTG CTCACCACTG TGGTGTGTGT CGTGCCCAGT 
TTAACACTCA GGTTCCTGAG GGTTGACCTG TCCCCCACCA CAACAGATAA GGTTCGTCAT 
CTGCAACTAT CCAGAAAGAA GGAAAAACCG CAGGAGCACA TTCCGAAGCC GGTACGCAGG 
ACCAGTTCTC GCCGTTCTGC CTACGCCTTC TCCCACCAGC AGGGCTACGG GGAGCTGATT 
ACCTCGGGCA AAAACATGCG GACAAGCTCA GTCTCGTCCG TACGTTCCCC TCAGTCGCCA 
CCACACTCCA GCCTCAAATG GATGGAAAAC ATACTGAAGG AAAAGAACAA AGTGGCGTGT 
GTCTCTGGTG AAAATGTCAT GAACAACGGG GAAGTCGACA GGGAACAAGA CGCCAAAACA 
GTTGACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605952.1
CDS	69..3680
Translation	MDFWRRNATVEKERRVKANARDHNRNFSYADNHIKTSKYNVFTFLPLNLFEQFQRVANAYFLVLLILQLIPQISSLSWFTTIVPLVLVLVTSAVKDARDDYFRHKSDRQVNNRQSQVIIGGRLQNEKWKNIQVGDVIKLENNQSVAADVLLLCSSEPCGLCYIETAALDGETNLKVRHALSVTSEMGDVAALMAFDGEVICETPNNKLDKFTGTLCWRGSRYPLDIGKMLLRGCVLRNTEWCFGMVVFAGQQTKLMQNCGKATFKRTSIDKLMNTLVLLIFAFLIFMGAILAIGNTIWESLVGVNFQDYLPWDTVQRNAVFSGFLTFWSYIIILNTVVPISLYVSMEILRLGHSYFINWDRRMYHAKTDTPAEARTTTLNEELGQVDFIFTDKTGTLTQNIMVFRKCSINGKTYGDVFDEFNQKVEITEKTVGVDFSFNPLRDPRFQFYDNSLLEAIELEEPAVQEFFRLLAVCHTVMAEEKTEGRLLYQAQSPDEGALVTAARNFGFVFRSRTPETISVCEMGRAVTYQLLAILDFDNVRKRMSVIVRNPEGDIKLYSKGADTIMFDLLDPSCENLMHVTSDHLGEFAADGLRTLALAYKDLDEEDFGVWMQKLHSASTVIEDREAQLAVTYEEIERGLKLLGATAIEDKLQEGVPETIASLHLADIKIWILTGDKLETAVNIGYSCSMLRDDMEEVFVVSGATSQDVQHQLREAKGQILATSRASWREDGGGPDAAADQPLYKEAVTEEFALVINGHSLAHALEPRLELLFLDVACLCKSIICSRVTPLQKAQVVELVKRCKRAVTLAIGDGANDVSMIQTAHIGIGISGQEGMQAVLASDYSFAQFRYLQRLLLVHGRWSYFRVCHFLYYFFYKNFAFTLVHFWYSFFCGFSAQTVYDQWFITHFNVIYTSLPVMGMGLFDQDVSDQHSLRYPSLYKPGQQNLLFNRRQFFLCALHGLTTSCLLFFIPYGAFAAMEKEDGTQISDQQTFAVTVATSLILVVSVEIGLEKHYWTAINQLFLGGSLTMYFAILFTMHSDGMFAVNQSHFPFVGTSRNCLSEANVWLVVVLTTVVCVVPSLTLRFLRVDLSPTTTDKVRHLQLSRKKEKPQEHIPKPVRRTSSRRSAYAFSHQQGYGELITSGKNMRTSSVSSVRSPQSPPHSSLKWMENILKEKNKVACVSGENVMNNGEVDREQDAKTVDT

Target ORF information:

RefSeq Version	XM_011607650.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes ATPase, class I, type 8B, member 4 (atp8b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607650.1

ORF Insert Sequence:

ATGGACTTCT GGAGGAGAAA CGCCACCGTC GAGAAGGAAC GGCGTGTGAA AGCGAACGCC 
CGGGACCACA ACAGAAACTT CTCCTATGCT GACAATCACA TCAAAACGTC CAAGTACAAC 
GTCTTCACCT TCCTGCCGCT CAATCTGTTC GAGCAGTTCC AGAGAGTCGC CAACGCTTAC 
TTCTTAGTGC TGCTGATACT GCAGTTGATC CCTCAAATTT CATCCCTCTC CTGGTTCACC 
ACCATCGTGC CTTTGGTGCT GGTGCTGGTC ACCTCGGCCG TCAAGGACGC CAGAGACGAC 
TACTTCCGAC ACAAGAGTGA TCGGCAAGTC AACAACCGCC AGTCGCAGGT CATCATCGGG 
GGACGCTTGC AGAATGAGAA GTGGAAGAAT ATTCAAGTGG GCGACGTCAT CAAGCTGGAG 
AATAATCAGA GTGTTGCCGC GGACGTCCTC CTTCTCTGCA GCAGCGAACC CTGCGGACTC 
TGTTACATCG AGACCGCGGC GCTCGATGGA GAGACCAATC TGAAAGTCCG CCACGCGCTG 
AGCGTCACCT CGGAGATGGG AGACGTTGCA GCCTTGATGG CCTTTGATGG AGAAGTCATC 
TGTGAAACTC CCAACAACAA GCTGGATAAA TTCACAGGAA CGTTGTGCTG GAGAGGGAGC 
AGATACCCTC TGGATATTGG CAAAATGCTG CTGCGAGGGT GCGTACTCAG GAACACGGAG 
TGGTGCTTTG GGATGGTTGT CTTTGCTGGA CAGCAAACCA AGCTAATGCA GAACTGTGGA 
AAGGCCACGT TTAAGAGGAC AAGTATCGAC AAACTGATGA ACACTCTGGT TTTACTGATC 
TTTGCCTTCC TTATCTTTAT GGGAGCTATT TTGGCCATTG GAAACACCAT TTGGGAGAGC 
TTGGTCGGAG TAAACTTCCA GGATTATTTG CCCTGGGACA CCGTTCAGAG GAACGCTGTT 
TTCTCCGGCT TCCTCACCTT CTGGTCCTAC ATCATCATCC TCAACACGGT GGTACCCATC 
TCTCTGTATG TCAGTATGGA GATTCTGAGG CTCGGCCACA GTTACTTCAT CAACTGGGAC 
CGGAGGATGT ACCATGCCAA GACGGACACT CCGGCAGAAG CTCGCACCAC CACCCTGAAC 
GAGGAGCTGG GCCAGGTGGA CTTCATCTTT ACGGACAAGA CGGGCACCCT CACGCAGAAC 
ATCATGGTCT TCAGAAAGTG CTCCATCAAT GGAAAAACAT ATGGGGATGT TTTTGATGAG 
TTCAACCAGA AGGTGGAAAT TACAGAGAAA ACTGTCGGCG TGGACTTCTC CTTCAACCCT 
CTCCGTGACC CAAGGTTTCA GTTTTATGAT AACAGCCTGC TGGAGGCCAT CGAGCTAGAG 
GAACCCGCCG TGCAGGAGTT CTTCAGGCTG CTGGCGGTGT GTCACACTGT CATGGCGGAG 
GAGAAGACTG AAGGGCGTTT GCTGTATCAG GCTCAGTCAC CCGACGAGGG AGCTCTGGTC 
ACAGCAGCAC GCAACTTCGG GTTCGTCTTC CGCTCCCGGA CCCCCGAGAC CATCAGCGTG 
TGTGAGATGG GACGAGCCGT GACCTACCAG CTGCTGGCCA TACTGGACTT CGACAACGTG 
CGGAAGAGAA TGAGCGTCAT TGTGAGAAAC CCAGAGGGTG ACATTAAACT CTACTCCAAA 
GGGGCTGACA CCATCATGTT TGACCTTCTG GATCCTTCCT GTGAAAACCT CATGCATGTC 
ACCTCAGACC ACCTCGGCGA ATTTGCAGCA GATGGGCTTC GCACGTTAGC TCTGGCTTAC 
AAGGACCTGG ATGAAGAGGA TTTTGGAGTC TGGATGCAGA AGCTTCACTC TGCCAGCACT 
GTCATCGAGG ACCGCGAGGC TCAGCTGGCG GTTACGTACG AGGAGATCGA ACGCGGCTTG 
AAGCTTCTAG GCGCCACAGC AATCGAGGAC AAACTTCAGG AAGGAGTCCC AGAAACAATC 
GCCAGCCTCC ATCTCGCTGA CATTAAGATC TGGATCCTCA CTGGGGACAA ACTCGAGACG 
GCCGTGAACA TCGGCTACTC CTGCAGCATG CTGCGAGACG ACATGGAGGA GGTGTTTGTC 
GTTTCCGGTG CGACGTCACA GGACGTGCAG CATCAGCTCA GGGAGGCTAA AGGTCAAATT 
TTGGCCACAA GTCGAGCAAG CTGGAGAGAA GATGGTGGGG GGCCAGACGC GGCTGCAGAT 
CAGCCTCTTT ACAAAGAAGC CGTTACAGAA GAGTTTGCGT TAGTCATCAA CGGACACAGT 
CTGGCTCACG CTCTGGAGCC ACGGCTGGAG CTGCTGTTCC TGGACGTGGC CTGCCTGTGC 
AAATCCATCA TCTGCAGCCG GGTGACGCCG CTGCAGAAGG CCCAGGTGGT GGAGCTGGTG 
AAGAGGTGCA AGAGGGCCGT CACGCTGGCC ATTGGAGACG GCGCCAATGA CGTCAGCATG 
ATCCAGACTG CTCACATCGG CATCGGCATC AGCGGTCAGG AGGGGATGCA GGCGGTTCTG 
GCCTCAGATT ACTCCTTCGC TCAGTTTCGG TACCTGCAGA GGCTCCTGCT GGTACACGGA 
CGCTGGTCCT ACTTCCGCGT TTGCCACTTC CTGTACTACT TCTTCTACAA GAACTTTGCC 
TTCACTCTGG TCCACTTCTG GTACAGCTTC TTCTGTGGTT TCTCAGCTCA GACTGTCTAT 
GACCAGTGGT TTATCACCCA CTTCAATGTC ATCTATACAT CTTTACCAGT TATGGGGATG 
GGACTTTTTG ATCAGGATGT CAGCGACCAG CACAGCCTTC GCTACCCAAG CTTGTACAAG 
CCGGGCCAGC AGAACCTTCT CTTCAACAGA CGGCAGTTCT TCCTGTGCGC GCTGCACGGC 
CTGACAACAT CCTGCCTCCT ATTCTTCATT CCCTATGGAG CCTTCGCTGC CATGGAGAAG 
GAGGACGGCA CCCAGATTTC CGATCAGCAA ACATTTGCCG TCACCGTGGC AACATCACTG 
ATCCTTGTCG TGAGTGTGGA GATTGGACTG GAGAAGCACT ACTGGACAGC CATCAATCAG 
CTGTTCCTGG GGGGAAGTCT CACCATGTAC TTCGCCATTT TGTTCACGAT GCACAGCGAC 
GGCATGTTTG CAGTGAATCA GAGCCATTTT CCATTTGTAG GGACATCACG CAACTGTCTG 
TCAGAGGCCA ACGTGTGGCT GGTGGTTGTG CTCACCACTG TGGTGTGTGT CGTGCCCAGT 
TTAACACTCA GGTTCCTGAG GGTTGACCTG TCCCCCACCA CAACAGATAA GGTTCGTCAT 
CTGCAACTAT CCAGAAAGAA GGAAAAACCG CAGGAGCACA TTCCGAAGCC GGTACGCAGG 
ACCAGTTCTC GCCGTTCTGC CTACGCCTTC TCCCACCAGC AGGGCTACGG GGAGCTGATT 
ACCTCGGGCA AAAACATGCG GACAAGCTCA GTCTCGTCCG TACGTTCCCC TCAGTCGCCA 
CCACACTCCA GCCTCAAATG GATGGAAAAC ATACTGAAGG AAAAGAACAA AGTGGCGTGT 
GTCTCTGGTG AAAATGTCAT GAACAACGGG GAAGTCGACA GGGAACAAGA CGCCAAAACA 
GTTGACACCT GA