LOC101067191 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101067191
Entrez Gene ID	101067191
Full Name	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003975757.3	XP_003975806.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_003975757.2	XP_003975806.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Pathways from BioSystems
Gene Ontology

The following LOC101067191 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101067191 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo62004	XM_003975757.3 Latest version!	Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo14604	XM_003975757.2 Latest version!	Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101067191 ( XM_003975757.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003975806.1 Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.

Price & Availability↑

CloneID	OTo62004
Clone ID Related Accession (Same CDS sequence)	XM_003975757.3
Accession Version	XM_003975757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003975757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCA GACCATCCTC GGGAGAGCTA TGGGGGATGC ACTTGATGCC CCCCAGCATC 
CTGGTGGAGT GCCTGCTGCC AAATGGCATG ATTCTCACGT TGGAGTGCCT CCGGGAGGCC 
ACGTTGATCA CAGTCAAGCA CGAGCTCTTC AAAGAGGCCA GGAAATACCC TCTTTACCAT 
CTGCTGCAGG AGGAGAGCTC CTACATCTTC GTCAGCGTCA CCCAAGAGGC AGAACGGGAG 
GAGTTTTACG ACGAGACGAG GAGGTTGTGC GACCTGCGGC TCTTCCAGGC GTTCCTCAAA 
GTCATTGAGC CGGTGGGCAA CAGGGAAGAG AAGATTCTCA ACAGAGAGAT CGGTTTTGCC 
ATCGGGATGC CGATATGCGA ATTTGATCTG GTGAAGGATC CAGAGGTGCA GGACTTCAGG 
CGCAACATTT TAAATGTTTG CAAAGAGGCG GTGGATCTCA GAGATTGCAA CGGGCCCCAC 
AGTAGAGCTC TGTACGTCTA CCCTCCCAAC GTGGAGTCGT CTCCGGAGCT TCCCAGGCAC 
ATCTACAACA AGCTGGACAA AGGTCAAATC ATTGTGGTCA TATGGGTCAT TGTGTCGCCC 
ACCAACGACA AGCAGAAATA CACCCTGAAG ATCAACCACG ATTACGTCCC GGAGCAGGTG 
ATCGCCGAAG CCATCCGCAA GAAGACGCGC AGCATGTTGC TGTCCCAGGA ACAGCTGAAG 
ATGTGCGTGC AGGAGTACCA GGGAAAGTAC ATCCTCAAAG TGTGCGGATG TGATGAGTAC 
TTACTGGAGA AGTACCCTCT GAGTCAGTAC AAGTATGTCC GCAGCTGCAT CATGCTGGGG 
CGCATGCCCA ACCTGATGCT GATGGCCAAA GACAGCCTGT ATTCCCAGCT GCCCATGGAC 
ATCTTCACCA TACCGTCCTA CGCCAGGAGG ATCTCCACAG CCACGCCCTA CATGAACGGC 
GAGACGGCCA CCAAAGCCTT GTGGACGATC AACGGAACAC TGAGGATCAA GATTCTTTGT 
GCCACTTATG TCAATGTCAA CATCAGAGAC ATCGACAAGA TCTACGTGAG GACTGGGATT 
TACCATGGTG GAGAGCAGCT GTGCGACAAC GTCAACACCC AAAGAGTGCC CTGCTCCAAC 
CCCAGGTGGA ACGAGTGGCT CAGCTACGAC ATGTACATTC CAGACATCCC TCGCGCCGCC 
CGGCTCTGCC TGTCCATCTG CTCTGTGAAA GGCAGGAAGG GAGCTAAAGA GGAACACTGT 
CCTCTGGCCT GGGGCAACAT CAACATGTTT GACTACACCC ACACGCTGGT GGCAGGAAAA 
ATGGCTCTCA ACCTGTGGCC TGTCCCTCAC GGCCTGGAAG ACCTGCTCAA CCCCATCGGC 
GTCACGGGCT CTAACCCCAA CAAGGAAACT CCATGTCTGG AGCTGGAGTT TGAACACTTC 
GGCTGCCCAG TTAAGTACCC TGACATGACC ATCATCGAAG AGCACGCCAA CTGGAATATA 
TCCAGAGAGC TGGGCTTCAA CTACTGCCAC ACCGGTTTGA GCAGCAGATT AGCGCGCGAC 
AACCCGGTGA CCGACGCTGA CAATGACCAG CTCCGCCAGG TGTGCACCAG AGACCCGCTG 
TCTGAGATCA CTGAGCAGGA GAAGGACTTC CTGTGGAGGC ACAGACAAGA CTGCCTCAAT 
ATGCCAGAGA TTCTTCCCAA GATCCTCCTC GCTGTGAAAT GGAACTCCAG AGATGAGATC 
GCGCAGATGT ACTGCCTCCT GAAGGACTGG CCCGCCATCA GGCCAGAACA GGCCATGGAG 
CTGCTGGACT GTAACTTCCC CGACCCCATG ATCCGAGAGT TTGCTGTAAA GTGCCTTGAG 
AAATATCTAA CGGATGACAA ACTCTCCCAG TACCTCATTC AGCTGGTTCA GGTTCTTAAA 
TACGAGCAGT ACCTCGATAA CCCACTCGCA CGCTTCCTTC TGAAAAGAGC GTTAACCAAT 
CAGAGGATAG GACATTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA CAAGACGGTG 
AGCCAGCGCT TCGGCCTGCT GCTGGAGTCG TACTGCCGGG CCTGTGGCAT GTATCTGAAG 
CACCTGAGCA GGCAGGTGGA GGCCATGGAG AAGCTCATCA ATCTCACTGA TCTCCTCAAA 
CAGGAGAAGA AAGATGAGGC GCAGAAGGTT CAAATGAAGT TCCTGGTGGA GCAAATGCGA 
AGGCCCGACT ACATGGATGC CCTGCAGAGT TTCACATCCC CACTTAATCC AGCACATCAG 
CTGGGCAACC TCCGCCTGGA AGAATGCAGG ATGATGTCAT CCGCCAAGCG ACCTCTGTGG 
CTGAACTGGG AGAATCCAGA TATGATGTCA GAGCTGCTTT TCCAGAACAA CGAAATCATT 
TTCAAAAATG GTGACGATCT GAGACAAGAC ATGCTGACGC TGCAGATAAT TAGGATAATG 
GAGAACATCT GGCAGAACCA GGGCCTTGAT CTCAGGATGC TGCCGTACGG CTGCCTGTCC 
ATCGGCGACT GCGTGGGCTT GATCGAGGTG GTGAGGAACT CCCACACCAT CATGCAGATC 
CAGTGCAAAG GAGGCCTGAA GGGGGCGCTG CAGTTCAACA GCCACACGTT ACACCAGTGG 
CTCAAGGATA AAAACAAAGG AGAGATGTAC GACCAGGCCA TAGACCTGTT CACCCGCTCC 
TGCGCAGGCT ACTGCGTCGC TACTTTTATT CTGGGCATTG GGGACAGACA CAACAGCAAC 
ATCATGGTCA AGGATGACGG ACAGCTGTTC CATATCGACT TTGGCCATTT TCTGGACCAC 
AAAAAGAAGA AATTTGGGTA CAAGAGGGAG CGGGTGCCCT TCGTGCTGAC GCAGGACTTC 
CTGATTGTTA TTAGTAAAGG AGCTCAGGAG TGCACCAAGA CGAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC GTACCTGGCA ATCAGACAGC ACGCCAACCT CTTCATCAAC 
CTCTTCTCCA TGATGCTGGG CTCCGGGATG CCCGAGCTGC AGTCCTTCGA TGACATCGCC 
TACATCAGAA AGACTCTGGC CCTGGACAAA TCGGAGCAGG AGGCCCTGGA CTACTTCATG 
AAGCAGATGA ACGACGCTCA CCACGGCGGC TGGACTACTA AAATGGACTG GATCTTCCAC 
ACGATCCGCC AGCACGCCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975806.1
CDS	398..3604
Translation	MPPRPSSGELWGMHLMPPSILVECLLPNGMILTLECLREATLITVKHELFKEARKYPLYHLLQEESSYIFVSVTQEAEREEFYDETRRLCDLRLFQAFLKVIEPVGNREEKILNREIGFAIGMPICEFDLVKDPEVQDFRRNILNVCKEAVDLRDCNGPHSRALYVYPPNVESSPELPRHIYNKLDKGQIIVVIWVIVSPTNDKQKYTLKINHDYVPEQVIAEAIRKKTRSMLLSQEQLKMCVQEYQGKYILKVCGCDEYLLEKYPLSQYKYVRSCIMLGRMPNLMLMAKDSLYSQLPMDIFTIPSYARRISTATPYMNGETATKALWTINGTLRIKILCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLSYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNINMFDYTHTLVAGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFEHFGCPVKYPDMTIIEEHANWNISRELGFNYCHTGLSSRLARDNPVTDADNDQLRQVCTRDPLSEITEQEKDFLWRHRQDCLNMPEILPKILLAVKWNSRDEIAQMYCLLKDWPAIRPEQAMELLDCNFPDPMIREFAVKCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLARFLLKRALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDLLKQEKKDEAQKVQMKFLVEQMRRPDYMDALQSFTSPLNPAHQLGNLRLEECRMMSSAKRPLWLNWENPDMMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEMYDQAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKSEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRQHAMN

Target ORF information:

RefSeq Version	XM_003975757.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975757.3

ORF Insert Sequence:

ATGCCTCCCA GACCATCCTC GGGAGAGCTA TGGGGGATGC ACTTGATGCC CCCCAGCATC 
CTGGTGGAGT GCCTGCTGCC AAATGGCATG ATTCTCACGT TGGAGTGCCT CCGGGAGGCC 
ACGTTGATCA CAGTCAAGCA CGAGCTCTTC AAAGAGGCCA GGAAATACCC TCTTTACCAT 
CTGCTGCAGG AGGAGAGCTC CTACATCTTC GTCAGCGTCA CCCAAGAGGC AGAACGGGAG 
GAGTTTTACG ACGAGACGAG GAGGTTGTGC GACCTGCGGC TCTTCCAGGC GTTCCTCAAA 
GTCATTGAGC CGGTGGGCAA CAGGGAAGAG AAGATTCTCA ACAGAGAGAT CGGTTTTGCC 
ATCGGGATGC CGATATGCGA ATTTGATCTG GTGAAGGATC CAGAGGTGCA GGACTTCAGG 
CGCAACATTT TAAATGTTTG CAAAGAGGCG GTGGATCTCA GAGATTGCAA CGGGCCCCAC 
AGTAGAGCTC TGTACGTCTA CCCTCCCAAC GTGGAGTCGT CTCCGGAGCT TCCCAGGCAC 
ATCTACAACA AGCTGGACAA AGGTCAAATC ATTGTGGTCA TATGGGTCAT TGTGTCGCCC 
ACCAACGACA AGCAGAAATA CACCCTGAAG ATCAACCACG ATTACGTCCC GGAGCAGGTG 
ATCGCCGAAG CCATCCGCAA GAAGACGCGC AGCATGTTGC TGTCCCAGGA ACAGCTGAAG 
ATGTGCGTGC AGGAGTACCA GGGAAAGTAC ATCCTCAAAG TGTGCGGATG TGATGAGTAC 
TTACTGGAGA AGTACCCTCT GAGTCAGTAC AAGTATGTCC GCAGCTGCAT CATGCTGGGG 
CGCATGCCCA ACCTGATGCT GATGGCCAAA GACAGCCTGT ATTCCCAGCT GCCCATGGAC 
ATCTTCACCA TACCGTCCTA CGCCAGGAGG ATCTCCACAG CCACGCCCTA CATGAACGGC 
GAGACGGCCA CCAAAGCCTT GTGGACGATC AACGGAACAC TGAGGATCAA GATTCTTTGT 
GCCACTTATG TCAATGTCAA CATCAGAGAC ATCGACAAGA TCTACGTGAG GACTGGGATT 
TACCATGGTG GAGAGCAGCT GTGCGACAAC GTCAACACCC AAAGAGTGCC CTGCTCCAAC 
CCCAGGTGGA ACGAGTGGCT CAGCTACGAC ATGTACATTC CAGACATCCC TCGCGCCGCC 
CGGCTCTGCC TGTCCATCTG CTCTGTGAAA GGCAGGAAGG GAGCTAAAGA GGAACACTGT 
CCTCTGGCCT GGGGCAACAT CAACATGTTT GACTACACCC ACACGCTGGT GGCAGGAAAA 
ATGGCTCTCA ACCTGTGGCC TGTCCCTCAC GGCCTGGAAG ACCTGCTCAA CCCCATCGGC 
GTCACGGGCT CTAACCCCAA CAAGGAAACT CCATGTCTGG AGCTGGAGTT TGAACACTTC 
GGCTGCCCAG TTAAGTACCC TGACATGACC ATCATCGAAG AGCACGCCAA CTGGAATATA 
TCCAGAGAGC TGGGCTTCAA CTACTGCCAC ACCGGTTTGA GCAGCAGATT AGCGCGCGAC 
AACCCGGTGA CCGACGCTGA CAATGACCAG CTCCGCCAGG TGTGCACCAG AGACCCGCTG 
TCTGAGATCA CTGAGCAGGA GAAGGACTTC CTGTGGAGGC ACAGACAAGA CTGCCTCAAT 
ATGCCAGAGA TTCTTCCCAA GATCCTCCTC GCTGTGAAAT GGAACTCCAG AGATGAGATC 
GCGCAGATGT ACTGCCTCCT GAAGGACTGG CCCGCCATCA GGCCAGAACA GGCCATGGAG 
CTGCTGGACT GTAACTTCCC CGACCCCATG ATCCGAGAGT TTGCTGTAAA GTGCCTTGAG 
AAATATCTAA CGGATGACAA ACTCTCCCAG TACCTCATTC AGCTGGTTCA GGTTCTTAAA 
TACGAGCAGT ACCTCGATAA CCCACTCGCA CGCTTCCTTC TGAAAAGAGC GTTAACCAAT 
CAGAGGATAG GACATTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA CAAGACGGTG 
AGCCAGCGCT TCGGCCTGCT GCTGGAGTCG TACTGCCGGG CCTGTGGCAT GTATCTGAAG 
CACCTGAGCA GGCAGGTGGA GGCCATGGAG AAGCTCATCA ATCTCACTGA TCTCCTCAAA 
CAGGAGAAGA AAGATGAGGC GCAGAAGGTT CAAATGAAGT TCCTGGTGGA GCAAATGCGA 
AGGCCCGACT ACATGGATGC CCTGCAGAGT TTCACATCCC CACTTAATCC AGCACATCAG 
CTGGGCAACC TCCGCCTGGA AGAATGCAGG ATGATGTCAT CCGCCAAGCG ACCTCTGTGG 
CTGAACTGGG AGAATCCAGA TATGATGTCA GAGCTGCTTT TCCAGAACAA CGAAATCATT 
TTCAAAAATG GTGACGATCT GAGACAAGAC ATGCTGACGC TGCAGATAAT TAGGATAATG 
GAGAACATCT GGCAGAACCA GGGCCTTGAT CTCAGGATGC TGCCGTACGG CTGCCTGTCC 
ATCGGCGACT GCGTGGGCTT GATCGAGGTG GTGAGGAACT CCCACACCAT CATGCAGATC 
CAGTGCAAAG GAGGCCTGAA GGGGGCGCTG CAGTTCAACA GCCACACGTT ACACCAGTGG 
CTCAAGGATA AAAACAAAGG AGAGATGTAC GACCAGGCCA TAGACCTGTT CACCCGCTCC 
TGCGCAGGCT ACTGCGTCGC TACTTTTATT CTGGGCATTG GGGACAGACA CAACAGCAAC 
ATCATGGTCA AGGATGACGG ACAGCTGTTC CATATCGACT TTGGCCATTT TCTGGACCAC 
AAAAAGAAGA AATTTGGGTA CAAGAGGGAG CGGGTGCCCT TCGTGCTGAC GCAGGACTTC 
CTGATTGTTA TTAGTAAAGG AGCTCAGGAG TGCACCAAGA CGAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC GTACCTGGCA ATCAGACAGC ACGCCAACCT CTTCATCAAC 
CTCTTCTCCA TGATGCTGGG CTCCGGGATG CCCGAGCTGC AGTCCTTCGA TGACATCGCC 
TACATCAGAA AGACTCTGGC CCTGGACAAA TCGGAGCAGG AGGCCCTGGA CTACTTCATG 
AAGCAGATGA ACGACGCTCA CCACGGCGGC TGGACTACTA AAATGGACTG GATCTTCCAC 
ACGATCCGCC AGCACGCCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101067191 ( XM_003975757.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003975806.1 Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.

Price & Availability↑

CloneID	OTo14604
Clone ID Related Accession (Same CDS sequence)	XM_003975757.2
Accession Version	XM_003975757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071169.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003975757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCA GACCATCCTC GGGAGAGCTA TGGGGGATGC ACTTGATGCC CCCCAGCATC 
CTGGTGGAGT GCCTGCTGCC AAATGGCATG ATTCTCACGT TGGAGTGCCT CCGGGAGGCC 
ACGTTGATCA CAGTCAAGCA CGAGCTCTTC AAAGAGGCCA GGAAATACCC TCTTTACCAT 
CTGCTGCAGG AGGAGAGCTC CTACATCTTC GTCAGCGTCA CCCAAGAGGC AGAACGGGAG 
GAGTTTTACG ACGAGACGAG GAGGTTGTGC GACCTGCGGC TCTTCCAGGC GTTCCTCAAA 
GTCATTGAGC CGGTGGGCAA CAGGGAAGAG AAGATTCTCA ACAGAGAGAT CGGTTTTGCC 
ATCGGGATGC CGATATGCGA ATTTGATCTG GTGAAGGATC CAGAGGTGCA GGACTTCAGG 
CGCAACATTT TAAATGTTTG CAAAGAGGCG GTGGATCTCA GAGATTGCAA CGGGCCCCAC 
AGTAGAGCTC TGTACGTCTA CCCTCCCAAC GTGGAGTCGT CTCCGGAGCT TCCCAGGCAC 
ATCTACAACA AGCTGGACAA AGGTCAAATC ATTGTGGTCA TATGGGTCAT TGTGTCGCCC 
ACCAACGACA AGCAGAAATA CACCCTGAAG ATCAACCACG ATTACGTCCC GGAGCAGGTG 
ATCGCCGAAG CCATCCGCAA GAAGACGCGC AGCATGTTGC TGTCCCAGGA ACAGCTGAAG 
ATGTGCGTGC AGGAGTACCA GGGAAAGTAC ATCCTCAAAG TGTGCGGATG TGATGAGTAC 
TTACTGGAGA AGTACCCTCT GAGTCAGTAC AAGTATGTCC GCAGCTGCAT CATGCTGGGG 
CGCATGCCCA ACCTGATGCT GATGGCCAAA GACAGCCTGT ATTCCCAGCT GCCCATGGAC 
ATCTTCACCA TACCGTCCTA CGCCAGGAGG ATCTCCACAG CCACGCCCTA CATGAACGGC 
GAGACGGCCA CCAAAGCCTT GTGGACGATC AACGGAACAC TGAGGATTAA GATTCTTTGT 
GCCACTTATG TCAATGTCAA CATCAGAGAC ATCGACAAGA TCTACGTGAG GACTGGGATT 
TACCACGGTG GAGAGCAGCT GTGCGACAAC GTCAACACCC AAAGAGTGCC CTGCTCCAAC 
CCCAGGTGGA ACGAGTGGCT CAGCTACGAC ATGTACATTC CAGACATCCC TCGCGCCGCC 
CGGCTCTGCC TGTCCATCTG CTCTGTGAAA GGCAGGAAGG GAGCTAAAGA GGAACACTGT 
CCTCTGGCCT GGGGCAACAT CAACATGTTT GACTACACCC ACACGCTGGT GGCAGGAAAA 
ATGGCTCTCA ACCTGTGGCC TGTCCCTCAC GGCCTGGAAG ACCTGCTCAA CCCCATCGGC 
GTCACGGGCT CTAACCCCAA CAAGGAAACT CCATGTCTGG AGCTGGAGTT TGAACACTTC 
GGCTGCCCAG TTAAGTACCC TGACATGACC ATCATCGAAG AGCACGCCAA CTGGAATATA 
TCCAGAGAGC TGGGCTTCAA CTACTGCCAC ACCGGTTTGA GCAGCAGATT AGCGCGCGAC 
AACCCGGTGA CCGACGCTGA CAATGACCAG CTCCGCCAGG TGTGCACCAG AGACCCGCTG 
TCTGAGATCA CTGAGCAGGA GAAGGACTTC CTGTGGAGGC ACAGACAAGA CTGCCTCAAT 
ATGCCAGAGA TTCTTCCCAA GATCCTCCTC GCTGTGAAAT GGAACTCCAG AGATGAGATC 
GCGCAGATGT ACTGCCTCCT GAAGGACTGG CCCGCCATCA GGCCAGAACA GGCCATGGAG 
CTGCTGGACT GTAACTTCCC CGACCCCATG ATCCGAGAGT TTGCTGTAAA GTGCCTTGAG 
AAATATCTAA CGGATGACAA ACTCTCCCAG TACCTCATTC AGCTGGTTCA GGTTCTTAAA 
TACGAACAGT ACCTCGATAA CCCACTCGCA CGCTTCCTTC TGAAAAGAGC GTTAACCAAT 
CAGAGGATAG GACATTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA CAAGACGGTG 
AGCCAGCGCT TCGGCCTGCT GCTGGAGTCG TACTGCCGGG CCTGTGGCAT GTATCTGAAG 
CACCTGAGCA GGCAGGTGGA GGCCATGGAG AAGCTCATCA ATCTCACTGA TCTCCTCAAA 
CAGGAGAAGA AAGATGAGGC GCAGAAGGTT CAAATGAAGT TCCTGGTGGA GCAAATGCGA 
AGGCCCGACT ACATGGATGC CCTGCAGAGT TTCACATCCC CACTTAATCC AGCACATCAG 
CTGGGCAACC TCCGCCTGGA AGAATGCAGG ATGATGTCAT CCGCCAAGCG ACCTCTGTGG 
CTGAACTGGG AGAATCCAGA TATGATGTCA GAGCTGCTTT TCCAGAACAA CGAAATCATT 
TTCAAAAATG GTGACGATCT GAGACAAGAC ATGCTGACGC TGCAGATAAT TAGGATAATG 
GAGAACATCT GGCAGAACCA GGGCCTTGAT CTCAGGATGC TGCCGTACGG CTGCCTGTCC 
ATCGGCGACT GCGTGGGCTT GATCGAGGTG GTGAGGAACT CCCACACCAT CATGCAGATC 
CAGTGCAAAG GAGGCCTGAA GGGGGCGCTG CAGTTCAACA GCCACACGTT ACACCAGTGG 
CTCAAGGATA AAAACAAAGG AGAGATGTAC GACCAGGCCA TAGACCTGTT CACCCGCTCC 
TGCGCAGGCT ACTGCGTCGC TACTTTTATT CTGGGCATTG GGGACAGACA CAACAGCAAC 
ATCATGGTCA AGGATGACGG ACAGCTGTTC CATATCGACT TTGGCCATTT TCTGGACCAC 
AAAAAGAAGA AATTTGGGTA CAAGAGGGAG CGGGTGCCCT TCGTGCTGAC GCAGGACTTC 
CTGATTGTTA TTAGTAAAGG AGCTCAGGAG TGCACCAAGA CGAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC GTACCTGGCA ATCAGACAGC ACGCCAACCT CTTCATCAAC 
CTCTTCTCCA TGATGCTGGG CTCCGGGATG CCCGAGCTGC AGTCCTTCGA TGACATCGCC 
TACATCAGAA AGACTCTGGC CCTGGACAAA TCGGAGCAGG AGGCCCTGGA CTACTTCATG 
AAGCAGATGA ACGACGCTCA CCACGGCGGC TGGACTACTA AAATGGACTG GATCTTCCAC 
ACGATCCGCC AGCACGCCAT GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975806.1
CDS	457..3663
Translation	MPPRPSSGELWGMHLMPPSILVECLLPNGMILTLECLREATLITVKHELFKEARKYPLYHLLQEESSYIFVSVTQEAEREEFYDETRRLCDLRLFQAFLKVIEPVGNREEKILNREIGFAIGMPICEFDLVKDPEVQDFRRNILNVCKEAVDLRDCNGPHSRALYVYPPNVESSPELPRHIYNKLDKGQIIVVIWVIVSPTNDKQKYTLKINHDYVPEQVIAEAIRKKTRSMLLSQEQLKMCVQEYQGKYILKVCGCDEYLLEKYPLSQYKYVRSCIMLGRMPNLMLMAKDSLYSQLPMDIFTIPSYARRISTATPYMNGETATKALWTINGTLRIKILCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLSYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNINMFDYTHTLVAGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFEHFGCPVKYPDMTIIEEHANWNISRELGFNYCHTGLSSRLARDNPVTDADNDQLRQVCTRDPLSEITEQEKDFLWRHRQDCLNMPEILPKILLAVKWNSRDEIAQMYCLLKDWPAIRPEQAMELLDCNFPDPMIREFAVKCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLARFLLKRALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDLLKQEKKDEAQKVQMKFLVEQMRRPDYMDALQSFTSPLNPAHQLGNLRLEECRMMSSAKRPLWLNWENPDMMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEMYDQAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKSEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRQHAMN

Target ORF information:

RefSeq Version	XM_003975757.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (LOC101067191), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975757.2

ORF Insert Sequence:

ATGCCTCCCA GACCATCCTC GGGAGAGCTA TGGGGGATGC ACTTGATGCC CCCCAGCATC 
CTGGTGGAGT GCCTGCTGCC AAATGGCATG ATTCTCACGT TGGAGTGCCT CCGGGAGGCC 
ACGTTGATCA CAGTCAAGCA CGAGCTCTTC AAAGAGGCCA GGAAATACCC TCTTTACCAT 
CTGCTGCAGG AGGAGAGCTC CTACATCTTC GTCAGCGTCA CCCAAGAGGC AGAACGGGAG 
GAGTTTTACG ACGAGACGAG GAGGTTGTGC GACCTGCGGC TCTTCCAGGC GTTCCTCAAA 
GTCATTGAGC CGGTGGGCAA CAGGGAAGAG AAGATTCTCA ACAGAGAGAT CGGTTTTGCC 
ATCGGGATGC CGATATGCGA ATTTGATCTG GTGAAGGATC CAGAGGTGCA GGACTTCAGG 
CGCAACATTT TAAATGTTTG CAAAGAGGCG GTGGATCTCA GAGATTGCAA CGGGCCCCAC 
AGTAGAGCTC TGTACGTCTA CCCTCCCAAC GTGGAGTCGT CTCCGGAGCT TCCCAGGCAC 
ATCTACAACA AGCTGGACAA AGGTCAAATC ATTGTGGTCA TATGGGTCAT TGTGTCGCCC 
ACCAACGACA AGCAGAAATA CACCCTGAAG ATCAACCACG ATTACGTCCC GGAGCAGGTG 
ATCGCCGAAG CCATCCGCAA GAAGACGCGC AGCATGTTGC TGTCCCAGGA ACAGCTGAAG 
ATGTGCGTGC AGGAGTACCA GGGAAAGTAC ATCCTCAAAG TGTGCGGATG TGATGAGTAC 
TTACTGGAGA AGTACCCTCT GAGTCAGTAC AAGTATGTCC GCAGCTGCAT CATGCTGGGG 
CGCATGCCCA ACCTGATGCT GATGGCCAAA GACAGCCTGT ATTCCCAGCT GCCCATGGAC 
ATCTTCACCA TACCGTCCTA CGCCAGGAGG ATCTCCACAG CCACGCCCTA CATGAACGGC 
GAGACGGCCA CCAAAGCCTT GTGGACGATC AACGGAACAC TGAGGATTAA GATTCTTTGT 
GCCACTTATG TCAATGTCAA CATCAGAGAC ATCGACAAGA TCTACGTGAG GACTGGGATT 
TACCACGGTG GAGAGCAGCT GTGCGACAAC GTCAACACCC AAAGAGTGCC CTGCTCCAAC 
CCCAGGTGGA ACGAGTGGCT CAGCTACGAC ATGTACATTC CAGACATCCC TCGCGCCGCC 
CGGCTCTGCC TGTCCATCTG CTCTGTGAAA GGCAGGAAGG GAGCTAAAGA GGAACACTGT 
CCTCTGGCCT GGGGCAACAT CAACATGTTT GACTACACCC ACACGCTGGT GGCAGGAAAA 
ATGGCTCTCA ACCTGTGGCC TGTCCCTCAC GGCCTGGAAG ACCTGCTCAA CCCCATCGGC 
GTCACGGGCT CTAACCCCAA CAAGGAAACT CCATGTCTGG AGCTGGAGTT TGAACACTTC 
GGCTGCCCAG TTAAGTACCC TGACATGACC ATCATCGAAG AGCACGCCAA CTGGAATATA 
TCCAGAGAGC TGGGCTTCAA CTACTGCCAC ACCGGTTTGA GCAGCAGATT AGCGCGCGAC 
AACCCGGTGA CCGACGCTGA CAATGACCAG CTCCGCCAGG TGTGCACCAG AGACCCGCTG 
TCTGAGATCA CTGAGCAGGA GAAGGACTTC CTGTGGAGGC ACAGACAAGA CTGCCTCAAT 
ATGCCAGAGA TTCTTCCCAA GATCCTCCTC GCTGTGAAAT GGAACTCCAG AGATGAGATC 
GCGCAGATGT ACTGCCTCCT GAAGGACTGG CCCGCCATCA GGCCAGAACA GGCCATGGAG 
CTGCTGGACT GTAACTTCCC CGACCCCATG ATCCGAGAGT TTGCTGTAAA GTGCCTTGAG 
AAATATCTAA CGGATGACAA ACTCTCCCAG TACCTCATTC AGCTGGTTCA GGTTCTTAAA 
TACGAACAGT ACCTCGATAA CCCACTCGCA CGCTTCCTTC TGAAAAGAGC GTTAACCAAT 
CAGAGGATAG GACATTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA CAAGACGGTG 
AGCCAGCGCT TCGGCCTGCT GCTGGAGTCG TACTGCCGGG CCTGTGGCAT GTATCTGAAG 
CACCTGAGCA GGCAGGTGGA GGCCATGGAG AAGCTCATCA ATCTCACTGA TCTCCTCAAA 
CAGGAGAAGA AAGATGAGGC GCAGAAGGTT CAAATGAAGT TCCTGGTGGA GCAAATGCGA 
AGGCCCGACT ACATGGATGC CCTGCAGAGT TTCACATCCC CACTTAATCC AGCACATCAG 
CTGGGCAACC TCCGCCTGGA AGAATGCAGG ATGATGTCAT CCGCCAAGCG ACCTCTGTGG 
CTGAACTGGG AGAATCCAGA TATGATGTCA GAGCTGCTTT TCCAGAACAA CGAAATCATT 
TTCAAAAATG GTGACGATCT GAGACAAGAC ATGCTGACGC TGCAGATAAT TAGGATAATG 
GAGAACATCT GGCAGAACCA GGGCCTTGAT CTCAGGATGC TGCCGTACGG CTGCCTGTCC 
ATCGGCGACT GCGTGGGCTT GATCGAGGTG GTGAGGAACT CCCACACCAT CATGCAGATC 
CAGTGCAAAG GAGGCCTGAA GGGGGCGCTG CAGTTCAACA GCCACACGTT ACACCAGTGG 
CTCAAGGATA AAAACAAAGG AGAGATGTAC GACCAGGCCA TAGACCTGTT CACCCGCTCC 
TGCGCAGGCT ACTGCGTCGC TACTTTTATT CTGGGCATTG GGGACAGACA CAACAGCAAC 
ATCATGGTCA AGGATGACGG ACAGCTGTTC CATATCGACT TTGGCCATTT TCTGGACCAC 
AAAAAGAAGA AATTTGGGTA CAAGAGGGAG CGGGTGCCCT TCGTGCTGAC GCAGGACTTC 
CTGATTGTTA TTAGTAAAGG AGCTCAGGAG TGCACCAAGA CGAGGGAGTT TGAAAGGTTC 
CAGGAGATGT GTTATAAGGC GTACCTGGCA ATCAGACAGC ACGCCAACCT CTTCATCAAC 
CTCTTCTCCA TGATGCTGGG CTCCGGGATG CCCGAGCTGC AGTCCTTCGA TGACATCGCC 
TACATCAGAA AGACTCTGGC CCTGGACAAA TCGGAGCAGG AGGCCCTGGA CTACTTCATG 
AAGCAGATGA ACGACGCTCA CCACGGCGGC TGGACTACTA AAATGGACTG GATCTTCCAC 
ACGATCCGCC AGCACGCCAT GAACTAA