LOC101065814 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101065814
Entrez Gene ID	101065814
Full Name	steroid 21-hydroxylase
Synonyms	cyp21A2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003966207.1	XP_003966256.1	steroid 21-hydroxylase
XM_029838965.1	XP_029694825.1	steroid 21-hydroxylase

Pathways from BioSystems
Gene Ontology

The following LOC101065814 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101065814 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo05186	XM_003966207.1 Latest version!	Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OTo69626	XM_029838965.1 Latest version!	Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101065814 ( XM_003966207.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003966256.1 Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.

Price & Availability↑

CloneID	OTo05186
Clone ID Related Accession (Same CDS sequence)	XM_003966207.1
Accession Version	XM_003966207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	steroid 21-hydroxylase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGTGG AAGTACTTGC AGCTATCGGA GCTGCGGCCC TCCTTGTTTT GCTGCTGATG 
TTACTGAGGG ACAAATGTGT TAAGAAATAC CACGAGGAAA GTGAGTATTC AGCTGTGAAA 
CGGAACCTGC CGCTGTCCCT CTGGCAGCTC CCTCTCCGGC CCTCATCTCC TCCCATCCCT 
GGTCCTCCCT GTCGCTTTCT TATCGGCAAC ATGACGGAGC TGATGCACGA CCACCTGCCG 
ATACACCTGA CCAACCTGGC AAAGCGGTAC GGGAACATCT ACCGCCTTAA ATGTGGAAAC 
ACAACCATGA TTGTGCTCAA CAGCAGCGAC ATCATAAGAG AGGCGCTGGT GAAGAAATGG 
TCCGACTTTG CTGGGAGAGC TGTCTCATAC ACTGCTGATA TCGTGTCCGG CGGCGGGCGC 
AACATCTCTC TGGGGGACTA CACAGAGGAG TGGAAGGCCC TTCGCCGCCT CGTCCACGGC 
GCCCTGCAGC GCTGTTGCAA GCACTCGTTG CACAATGTGA TAGAGAGACA AGCGCTCCAA 
CTGAGAAAGG TGTTGGTTGA CTATCGGGGC GGCGCCGTGG ATCTGTCTGA GGATTTTACT 
GTGGCCGCCA GTAACGTCAT CATTACTTTG GTGTTTGGAA AAGAATACGA CAAAAGCTCC 
TCAGAGCTGC AACAGCTGCA CCGTTGTCTG AATGAGATTG TGGCTCTCTG GGGCTCCACT 
TGGATCTCTG CTCTTGATAC GTTCCCTCTG CTCAGAAAAT TCCCCAATCC CGTGTTTTCT 
CGTCTCCTGA GGGAGGTCAG CAGGAGAGAT GAAATCATCA GGAAACATCT CAACCAGTTC 
AAGTCAGAGG GGCACAGAAG GACAGATGTG ATCACAGGAT CTCTCCTTGA AGGGGTTGAC 
AGGCATCAGA CAGAACCTGG AGTGGTCCTG ACAGACATGC ATGTCCACAT GGCAACTGTA 
GATCTTCTCA TCGGGGGGTC CGAGACCACT GCAGCCTGGC TGAACTGGAC TGTGGCTTTC 
CTCTTGCACA GACCAGAGTT TCAGACAAAG GTCTACGAGG AGCTGTGCAC GGTGCTGGAG 
GGACGTTACC CCAAATACAG TGACAGACAG AGGCTCCCCA TCCTTTGCTC GCTCATACAT 
GAAGTTCTCA GGCTCAGGCC CGTCGCCCCA CTGGCGGTGC CGCACAAAGC CATCAGAGAC 
AGCAGTATTG CAGGTTACTT CATCCCCAGA AACACAATCA TCATCCCTAA TCTGTTTGGA 
GCTCACCACG ATCCCGAAGT TTGGTCGGAC CCATATAGCT TCAAACCTGA GCGCTTTCTG 
GAGGGAGGAG GGGGCTCCAC CCGCGCCCTG ATCCCCTTCG GAGGCGGAGC GCGACTCTGT 
CTGGGGGAGA CTGTGGCTAA GATGGAGCTC TTTCTCTTCA CTGCATACCT GCTGAGAGAT 
TTTTGCTTTG TCCTTCCCGA CAGTGAAGCA CCTCTGCCCG ACCTGAGGGG GGTGGCTAGC 
GTCGTGCTTA AAATCAAGTC CTTTACAGTC ATAGCACGTC CCCGGACTGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003966256.1
CDS	1..1557
Translation	MSVEVLAAIGAAALLVLLLMLLRDKCVKKYHEESEYSAVKRNLPLSLWQLPLRPSSPPIPGPPCRFLIGNMTELMHDHLPIHLTNLAKRYGNIYRLKCGNTTMIVLNSSDIIREALVKKWSDFAGRAVSYTADIVSGGGRNISLGDYTEEWKALRRLVHGALQRCCKHSLHNVIERQALQLRKVLVDYRGGAVDLSEDFTVAASNVIITLVFGKEYDKSSSELQQLHRCLNEIVALWGSTWISALDTFPLLRKFPNPVFSRLLREVSRRDEIIRKHLNQFKSEGHRRTDVITGSLLEGVDRHQTEPGVVLTDMHVHMATVDLLIGGSETTAAWLNWTVAFLLHRPEFQTKVYEELCTVLEGRYPKYSDRQRLPILCSLIHEVLRLRPVAPLAVPHKAIRDSSIAGYFIPRNTIIIPNLFGAHHDPEVWSDPYSFKPERFLEGGGGSTRALIPFGGGARLCLGETVAKMELFLFTAYLLRDFCFVLPDSEAPLPDLRGVASVVLKIKSFTVIARPRTGP

Target ORF information:

RefSeq Version	XM_003966207.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003966207.1

ORF Insert Sequence:

ATGTCAGTGG AAGTACTTGC AGCTATCGGA GCTGCGGCCC TCCTTGTTTT GCTGCTGATG 
TTACTGAGGG ACAAATGTGT TAAGAAATAC CACGAGGAAA GTGAGTATTC AGCTGTGAAA 
CGGAACCTGC CGCTGTCCCT CTGGCAGCTC CCTCTCCGGC CCTCATCTCC TCCCATCCCT 
GGTCCTCCCT GTCGCTTTCT TATCGGCAAC ATGACGGAGC TGATGCACGA CCACCTGCCG 
ATACACCTGA CCAACCTGGC AAAGCGGTAC GGGAACATCT ACCGCCTTAA ATGTGGAAAC 
ACAACCATGA TTGTGCTCAA CAGCAGCGAC ATCATAAGAG AGGCGCTGGT GAAGAAATGG 
TCCGACTTTG CTGGGAGAGC TGTCTCATAC ACTGCTGATA TCGTGTCCGG CGGCGGGCGC 
AACATCTCTC TGGGGGACTA CACAGAGGAG TGGAAGGCCC TTCGCCGCCT CGTCCACGGC 
GCCCTGCAGC GCTGTTGCAA GCACTCGTTG CACAATGTGA TAGAGAGACA AGCGCTCCAA 
CTGAGAAAGG TGTTGGTTGA CTATCGGGGC GGCGCCGTGG ATCTGTCTGA GGATTTTACT 
GTGGCCGCCA GTAACGTCAT CATTACTTTG GTGTTTGGAA AAGAATACGA CAAAAGCTCC 
TCAGAGCTGC AACAGCTGCA CCGTTGTCTG AATGAGATTG TGGCTCTCTG GGGCTCCACT 
TGGATCTCTG CTCTTGATAC GTTCCCTCTG CTCAGAAAAT TCCCCAATCC CGTGTTTTCT 
CGTCTCCTGA GGGAGGTCAG CAGGAGAGAT GAAATCATCA GGAAACATCT CAACCAGTTC 
AAGTCAGAGG GGCACAGAAG GACAGATGTG ATCACAGGAT CTCTCCTTGA AGGGGTTGAC 
AGGCATCAGA CAGAACCTGG AGTGGTCCTG ACAGACATGC ATGTCCACAT GGCAACTGTA 
GATCTTCTCA TCGGGGGGTC CGAGACCACT GCAGCCTGGC TGAACTGGAC TGTGGCTTTC 
CTCTTGCACA GACCAGAGTT TCAGACAAAG GTCTACGAGG AGCTGTGCAC GGTGCTGGAG 
GGACGTTACC CCAAATACAG TGACAGACAG AGGCTCCCCA TCCTTTGCTC GCTCATACAT 
GAAGTTCTCA GGCTCAGGCC CGTCGCCCCA CTGGCGGTGC CGCACAAAGC CATCAGAGAC 
AGCAGTATTG CAGGTTACTT CATCCCCAGA AACACAATCA TCATCCCTAA TCTGTTTGGA 
GCTCACCACG ATCCCGAAGT TTGGTCGGAC CCATATAGCT TCAAACCTGA GCGCTTTCTG 
GAGGGAGGAG GGGGCTCCAC CCGCGCCCTG ATCCCCTTCG GAGGCGGAGC GCGACTCTGT 
CTGGGGGAGA CTGTGGCTAA GATGGAGCTC TTTCTCTTCA CTGCATACCT GCTGAGAGAT 
TTTTGCTTTG TCCTTCCCGA CAGTGAAGCA CCTCTGCCCG ACCTGAGGGG GGTGGCTAGC 
GTCGTGCTTA AAATCAAGTC CTTTACAGTC ATAGCACGTC CCCGGACTGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101065814 ( XM_029838965.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029694825.1 Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.

Price & Availability↑

CloneID	OTo69626
Clone ID Related Accession (Same CDS sequence)	XM_029838965.1
Accession Version	XM_029838965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	steroid 21-hydroxylase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGG AAGTACTTGC AGCTATCGGA GCTGCGGCCC TCCTTGTTTT GCTGCTGATG 
TTACTGAGGG ACAAATGTGT TAAGAAATAC CACGAGGAAA CTGTGAAACG GAACCTGCCG 
CTGTCCCTCT GGCAGCTCCC TCTCCGGCCC TCATCTCCTC CCATCCCTGG TCCTCCCTGT 
CGCTTTCTTA TCGGCAACAT GACGGAGCTG ATGCACGACC ACCTGCCGAT ACACCTGACC 
AACCTGGCAA AGCGGTACGG GAACATCTAC CGCCTTAAAT GTGGAAACAC AACCATGATT 
GTGCTCAACA GCAGCGACAT CATAAGAGAG GCGCTGGTGA AGAAATGGTC CGACTTTGCT 
GGGAGAGCTG TCTCATACAC TGCTGATATC GTGTCCGGCG GCGGGCGCAA CATCTCTCTG 
GGGGACTACA CAGAGGAGTG GAAGGCCCTT CGCCGCCTCG TCCACGGCGC CCTGCAGCGC 
TGTTGCAAGC ACTCGTTGCA CAATGTGATA GAGAGACAAG CGCTCCAACT GAGAAAGGTG 
TTGGTTGACT ATCGGGGCGG CGCCGTGGAT CTGTCTGAGG ATTTTACTGT GGCCGCCAGT 
AACGTCATCA TTACTTTGGT GTTTGGAAAA GAATACGACA AAAGCTCCTC AGAGCTGCAA 
CAGCTGCACC GTTGTCTGAA TGAGATTGTG GCTCTCTGGG GCTCCACTTG GATCTCTGCT 
CTTGATACGT TCCCTCTGCT CAGAAAATTC CCCAATCCCG TGTTTTCTCG TCTCCTGAGG 
GAGGTCAGCA GGAGAGATGA AATCATCAGG AAACATCTCA ACCAGTTCAA GTCAGAGGGG 
CACAGAAGGA CAGATGTGAT CACAGGATCT CTCCTTGAAG GGGTTGACAG GCATCAGACA 
GAACCTGGAG TGGTCCTGAC AGACATGCAT GTCCACATGG CAACTGTAGA TCTTCTCATC 
GGGGGGTCCG AGACCACTGC AGCCTGGCTG AACTGGACTG TGGCTTTCCT CTTGCACAGA 
CCAGAGTTTC AGACAAAGGT CTACGAGGAG CTGTGCACGG TGCTGGAGGG ACGTTACCCC 
AAATACAGTG ACAGACAGAG GCTCCCCATC CTTTGCTCGC TCATACATGA AGTTCTCAGG 
CTCAGGCCCG TCGCCCCACT GGCGGTGCCG CACAAAGCCA TCAGAGACAG CAGTATTGCA 
GGTTACTTCA TCCCCAGAAA CACAATCATC ATCCCTAATC TGTTTGGAGC TCACCACGAT 
CCCGAAGTTT GGTCGGACCC ATATAGCTTC AAACCTGAGC GCTTTCTGGA GGGAGGAGGG 
GGCTCCACCC GCGCCCTGAT CCCCTTCGGA GGCGGAGCGC GACTCTGTCT GGGGGAGACT 
GTGGCTAAGA TGGAGCTCTT TCTCTTCACT GCATACCTGC TGAGAGATTT TTGCTTTGTC 
CTTCCCGACA GTGAAGCACC TCTGCCCGAC CTGAGGGGGG TGGCTAGCGT CGTGCTTAAA 
ATCAAGTCCT TTACAGTCAT AGCACGTCCC CGGACTGGGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029694825.1
CDS	10..1554
Translation	MSVEVLAAIGAAALLVLLLMLLRDKCVKKYHEETVKRNLPLSLWQLPLRPSSPPIPGPPCRFLIGNMTELMHDHLPIHLTNLAKRYGNIYRLKCGNTTMIVLNSSDIIREALVKKWSDFAGRAVSYTADIVSGGGRNISLGDYTEEWKALRRLVHGALQRCCKHSLHNVIERQALQLRKVLVDYRGGAVDLSEDFTVAASNVIITLVFGKEYDKSSSELQQLHRCLNEIVALWGSTWISALDTFPLLRKFPNPVFSRLLREVSRRDEIIRKHLNQFKSEGHRRTDVITGSLLEGVDRHQTEPGVVLTDMHVHMATVDLLIGGSETTAAWLNWTVAFLLHRPEFQTKVYEELCTVLEGRYPKYSDRQRLPILCSLIHEVLRLRPVAPLAVPHKAIRDSSIAGYFIPRNTIIIPNLFGAHHDPEVWSDPYSFKPERFLEGGGGSTRALIPFGGGARLCLGETVAKMELFLFTAYLLRDFCFVLPDSEAPLPDLRGVASVVLKIKSFTVIARPRTGP

Target ORF information:

RefSeq Version	XM_029838965.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes steroid 21-hydroxylase (LOC101065814), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029838965.1

ORF Insert Sequence:

ATGTCAGTGG AAGTACTTGC AGCTATCGGA GCTGCGGCCC TCCTTGTTTT GCTGCTGATG 
TTACTGAGGG ACAAATGTGT TAAGAAATAC CACGAGGAAA CTGTGAAACG GAACCTGCCG 
CTGTCCCTCT GGCAGCTCCC TCTCCGGCCC TCATCTCCTC CCATCCCTGG TCCTCCCTGT 
CGCTTTCTTA TCGGCAACAT GACGGAGCTG ATGCACGACC ACCTGCCGAT ACACCTGACC 
AACCTGGCAA AGCGGTACGG GAACATCTAC CGCCTTAAAT GTGGAAACAC AACCATGATT 
GTGCTCAACA GCAGCGACAT CATAAGAGAG GCGCTGGTGA AGAAATGGTC CGACTTTGCT 
GGGAGAGCTG TCTCATACAC TGCTGATATC GTGTCCGGCG GCGGGCGCAA CATCTCTCTG 
GGGGACTACA CAGAGGAGTG GAAGGCCCTT CGCCGCCTCG TCCACGGCGC CCTGCAGCGC 
TGTTGCAAGC ACTCGTTGCA CAATGTGATA GAGAGACAAG CGCTCCAACT GAGAAAGGTG 
TTGGTTGACT ATCGGGGCGG CGCCGTGGAT CTGTCTGAGG ATTTTACTGT GGCCGCCAGT 
AACGTCATCA TTACTTTGGT GTTTGGAAAA GAATACGACA AAAGCTCCTC AGAGCTGCAA 
CAGCTGCACC GTTGTCTGAA TGAGATTGTG GCTCTCTGGG GCTCCACTTG GATCTCTGCT 
CTTGATACGT TCCCTCTGCT CAGAAAATTC CCCAATCCCG TGTTTTCTCG TCTCCTGAGG 
GAGGTCAGCA GGAGAGATGA AATCATCAGG AAACATCTCA ACCAGTTCAA GTCAGAGGGG 
CACAGAAGGA CAGATGTGAT CACAGGATCT CTCCTTGAAG GGGTTGACAG GCATCAGACA 
GAACCTGGAG TGGTCCTGAC AGACATGCAT GTCCACATGG CAACTGTAGA TCTTCTCATC 
GGGGGGTCCG AGACCACTGC AGCCTGGCTG AACTGGACTG TGGCTTTCCT CTTGCACAGA 
CCAGAGTTTC AGACAAAGGT CTACGAGGAG CTGTGCACGG TGCTGGAGGG ACGTTACCCC 
AAATACAGTG ACAGACAGAG GCTCCCCATC CTTTGCTCGC TCATACATGA AGTTCTCAGG 
CTCAGGCCCG TCGCCCCACT GGCGGTGCCG CACAAAGCCA TCAGAGACAG CAGTATTGCA 
GGTTACTTCA TCCCCAGAAA CACAATCATC ATCCCTAATC TGTTTGGAGC TCACCACGAT 
CCCGAAGTTT GGTCGGACCC ATATAGCTTC AAACCTGAGC GCTTTCTGGA GGGAGGAGGG 
GGCTCCACCC GCGCCCTGAT CCCCTTCGGA GGCGGAGCGC GACTCTGTCT GGGGGAGACT 
GTGGCTAAGA TGGAGCTCTT TCTCTTCACT GCATACCTGC TGAGAGATTT TTGCTTTGTC 
CTTCCCGACA GTGAAGCACC TCTGCCCGAC CTGAGGGGGG TGGCTAGCGT CGTGCTTAAA 
ATCAAGTCCT TTACAGTCAT AGCACGTCCC CGGACTGGGC CTTAA