smg8 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smg8
Entrez Gene ID	107386629
Full Name	SMG8, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015961156.1	XP_015816642.1	protein SMG8

Pathways from BioSystems
Gene Ontology

The following smg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo18153	XM_015961156.1 Latest version!	Nothobranchius furzeri SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg8 ( XM_015961156.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015816642.1 Nothobranchius furzeri SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.

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CloneID	ONo18153
Clone ID Related Accession (Same CDS sequence)	XM_015961156.1
Accession Version	XM_015961156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	protein SMG8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAA CGAACATGTC TCTCCCCGTG AGTGTTGGAA CTCTGCTGCA GTCGGGCCTG 
CCGGAGGACG CTGCTTACCG GGACGACGGC CTCTGTGTTG TCGGTATTTT CGGGAAAAAC 
AACACGCAGC CCGGACCGCT TAAAGACTCT CTTATCAACA TATTGGAAGA CAAACATATC 
TTCTCCGTGT TCGGTGAACC AGAGAACGAC GACACAGCAG GCAGCCACAT CCAGGCCTTC 
TACAACCAGG AGAACCGGGT ACTGTTCCTG CTGTTGTCCT CCGTGTCCGA CAGCCGGCAG 
CTGCTCCGGG CCTGTCGGTC TCTGAGCGCT GGGATCAGCC ACTCCGACGC TCACGACTTC 
TGGAAAGGGC TGGACCGACA GCACTGTCTC CACCTGCTCT ACATGTTTTC AGTGTGCCAC 
GTCCTGCTGC TGGTCCACCC CAGTCAGACC TTCGACGTGA CCTACGACAA GCTCTTCAGA 
GCCCTGGACG CCCTGCGGCA GAAGGTGCTG CCCCTGATCC GGGCTGCCAT CAAGGACTGT 
CCTGTATCCA AAGAGTGGAA GCTGAACTGC AGACCCTGTC CCCCACGGCT GCTTTTTGTC 
TTCCAGATGA CCGCCACTCT CAAGGTTTCT GCAAATGGAT TTGAGTCTGG AGGAAATCCC 
GACAAACCCA AAAAGCATTC TCCCAGGAGG AGGCTGCAGC ATGCTCTGGA GGATCAGATT 
TACCGGATCT TCCGTAAAAG CAGAGTTCTG ACCAACCAGA GCAGTAACTG TTTGTTCACC 
GTGCCTGCAA ACCAGGCGTT TGTGTACGTG ATGCCAACAG CAGACAAGGA CCCAGTAGGT 
ACTTTGCTCA GCCAACTGCA GTCAAACTGC ATGCTGTCTG AGCAGGACTC CCCCGTGCCC 
GTGTCAGGGC CGAGGCGATA CCAGCAGATG CGCCGATCGG CCCGGCAGCC CTGCAGCGGT 
GGCGAGCTGC GTGGCTTATC GTTGGGCGGT CAGCTAGTGG ATTGCAGCTT GAGGGAATTC 
CTTTGGCAGC ACGTTGAACT GGTGCTGACC AAGAAAGGTT TTGATGACAG CGTTGGTCGC 
AACCCACAGC CTTCACACTT TGAGCTGCCG ACGTACACCA AATGGATCCA GGTCTCCTCC 
AGACTCTACC AAGTCCTAAT CACCAACACG GATGAAGAAC TGGCTGAGTT GGCCACAAAA 
GTCCAAAGCC AGGTGAAGGT TTTGGATGGA TTTCTTGATG CTGATACAAA GTTTTCAGAA 
AACAGGTGCC AGAAAGCTCT GCCACTGGCT CACAGCGCCT ACCAGTCTAA CCTTCCACAC 
AACTACACCA CAACAGTACA CAAAAACCAG CTGGCTCAGG CCCTGCGGGT GTACAGCCAG 
CACGCCCGAG GCGTGGCTTT CCAGCGGTAT GCCCTGCAGC TCCACGAGGA CTGTTACAAG 
TTCTGGAGCA ATGGCCACCA GCTGTGTGAA GAGAGAAGTC TCACAGACCA GCACTGTGTT 
CACAAGTTCC ACCTCCTGCC TCAACCTGGA GAGAAAGCAG ACCTAGATCA CAACCCACCC 
ATCATGAACC ACAACAGCAG GGGGCGCTCC ACCAGCTCCT GTAACTGTGG GCGGAAACAG 
GCTCCTCGGG AGGATCCCTT TGACATCCAG TCTGCAAATT ACGACTTCTA CCAGATGCTG 
GAGGAGAAGT GCTGTGGGAA GCTGGAGAGG ATCGAGTTTC CTGTGTTCCA GCCCAGCACT 
CCGGACCCAG CGCCAGCCAG CAACCAGACT CAGCGGCTCC CATCTGAAAC AGACACAGAG 
AGGCAGAAGG AAGCGAGCAC AGCTCAGAGC CACACACCTG GAGAAACCAG TCTCAGCCTG 
GCTCTCAGTC TGGGTCAGTC CACAGACAGC CTGGAGCCCT ACGGGGATGG AGACGCACCT 
GAAAACCAGG TTCAGCCCAA GAGGCCTAGC CTCGTGGACC GTCAGCCCTC CACTGTGGAG 
TACTTACCAG GTATGATGCA CTCCAGCTGT CCAAAAGGCC TTCTCCCAAA GTTCTCCAGC 
TGGTCACTGA TCAAACTGGG CCAAGCGAAG TCTTACAACT GCCACACTGG TCTGGAACAG 
CCAGGATTCC TCCCCGGCTC TTCCTTCCTC TTGCCGTGGG ACTTGGTGAT CCGGTCTCGT 
TCGGTGGAGG ACGCAGGAGT GATGGAGCCT TTGGACGGAG GCGCGTCATC ATGGCCAGCT 
CCCAACAAGA GCCTGGTTGG AAAGCGAGGC AGCACCGGTG GGCTCGGTAG GAGTCGCAGG 
AGGGACGACA TGGCTCGGGT CTTTGTAGGG TTTGAGTACG AGGACAGCAG AGGACGACGC 
TTCATTAGCT GTGGGCCTGA TAAGATAGTA AAGGCTCTGG GGCCAGGGGG CGCCAAAGAT 
CCCGCCACCC GAGTCTTAAA CACAGACATG CCGCTCTACG TCCCATCTCC ATCACAGGGG 
CGAGGCCTGA AGCCGCACTT CGCACAGCTG ACTCGTCTCT TTATTGTGGT TCCCGACACG 
CCGCTGGAGA TCACGCTGAA CCCCCAGGTC CAACCCGGAC TTCCTCCTTG CCCCATCTTT 
CACCCGGAGC AGCCGGAGGT TGTGTTGCCC CCCGACAGCT TCTGGGTTCT GCGGTTTCCT 
TATTCCTACG CGACTGAGCG CGGCCCCTTG TTCCCCCCCA AAGAGAACCA ACCCCTCAAC 
AACTACAAGG TGCTGCGAGG CATCCTGAGA GCCTCCACAG CCAACCCTCC GCCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015816642.1
CDS	163..2922
Translation	MKETNMSLPVSVGTLLQSGLPEDAAYRDDGLCVVGIFGKNNTQPGPLKDSLINILEDKHIFSVFGEPENDDTAGSHIQAFYNQENRVLFLLLSSVSDSRQLLRACRSLSAGISHSDAHDFWKGLDRQHCLHLLYMFSVCHVLLLVHPSQTFDVTYDKLFRALDALRQKVLPLIRAAIKDCPVSKEWKLNCRPCPPRLLFVFQMTATLKVSANGFESGGNPDKPKKHSPRRRLQHALEDQIYRIFRKSRVLTNQSSNCLFTVPANQAFVYVMPTADKDPVGTLLSQLQSNCMLSEQDSPVPVSGPRRYQQMRRSARQPCSGGELRGLSLGGQLVDCSLREFLWQHVELVLTKKGFDDSVGRNPQPSHFELPTYTKWIQVSSRLYQVLITNTDEELAELATKVQSQVKVLDGFLDADTKFSENRCQKALPLAHSAYQSNLPHNYTTTVHKNQLAQALRVYSQHARGVAFQRYALQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHLLPQPGEKADLDHNPPIMNHNSRGRSTSSCNCGRKQAPREDPFDIQSANYDFYQMLEEKCCGKLERIEFPVFQPSTPDPAPASNQTQRLPSETDTERQKEASTAQSHTPGETSLSLALSLGQSTDSLEPYGDGDAPENQVQPKRPSLVDRQPSTVEYLPGMMHSSCPKGLLPKFSSWSLIKLGQAKSYNCHTGLEQPGFLPGSSFLLPWDLVIRSRSVEDAGVMEPLDGGASSWPAPNKSLVGKRGSTGGLGRSRRRDDMARVFVGFEYEDSRGRRFISCGPDKIVKALGPGGAKDPATRVLNTDMPLYVPSPSQGRGLKPHFAQLTRLFIVVPDTPLEITLNPQVQPGLPPCPIFHPEQPEVVLPPDSFWVLRFPYSYATERGPLFPPKENQPLNNYKVLRGILRASTANPPPQ

Target ORF information:

RefSeq Version	XM_015961156.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015961156.1

ORF Insert Sequence:

ATGAAGGAAA CGAACATGTC TCTCCCCGTG AGTGTTGGAA CTCTGCTGCA GTCGGGCCTG 
CCGGAGGACG CTGCTTACCG GGACGACGGC CTCTGTGTTG TCGGTATTTT CGGGAAAAAC 
AACACGCAGC CCGGACCGCT TAAAGACTCT CTTATCAACA TATTGGAAGA CAAACATATC 
TTCTCCGTGT TCGGTGAACC AGAGAACGAC GACACAGCAG GCAGCCACAT CCAGGCCTTC 
TACAACCAGG AGAACCGGGT ACTGTTCCTG CTGTTGTCCT CCGTGTCCGA CAGCCGGCAG 
CTGCTCCGGG CCTGTCGGTC TCTGAGCGCT GGGATCAGCC ACTCCGACGC TCACGACTTC 
TGGAAAGGGC TGGACCGACA GCACTGTCTC CACCTGCTCT ACATGTTTTC AGTGTGCCAC 
GTCCTGCTGC TGGTCCACCC CAGTCAGACC TTCGACGTGA CCTACGACAA GCTCTTCAGA 
GCCCTGGACG CCCTGCGGCA GAAGGTGCTG CCCCTGATCC GGGCTGCCAT CAAGGACTGT 
CCTGTATCCA AAGAGTGGAA GCTGAACTGC AGACCCTGTC CCCCACGGCT GCTTTTTGTC 
TTCCAGATGA CCGCCACTCT CAAGGTTTCT GCAAATGGAT TTGAGTCTGG AGGAAATCCC 
GACAAACCCA AAAAGCATTC TCCCAGGAGG AGGCTGCAGC ATGCTCTGGA GGATCAGATT 
TACCGGATCT TCCGTAAAAG CAGAGTTCTG ACCAACCAGA GCAGTAACTG TTTGTTCACC 
GTGCCTGCAA ACCAGGCGTT TGTGTACGTG ATGCCAACAG CAGACAAGGA CCCAGTAGGT 
ACTTTGCTCA GCCAACTGCA GTCAAACTGC ATGCTGTCTG AGCAGGACTC CCCCGTGCCC 
GTGTCAGGGC CGAGGCGATA CCAGCAGATG CGCCGATCGG CCCGGCAGCC CTGCAGCGGT 
GGCGAGCTGC GTGGCTTATC GTTGGGCGGT CAGCTAGTGG ATTGCAGCTT GAGGGAATTC 
CTTTGGCAGC ACGTTGAACT GGTGCTGACC AAGAAAGGTT TTGATGACAG CGTTGGTCGC 
AACCCACAGC CTTCACACTT TGAGCTGCCG ACGTACACCA AATGGATCCA GGTCTCCTCC 
AGACTCTACC AAGTCCTAAT CACCAACACG GATGAAGAAC TGGCTGAGTT GGCCACAAAA 
GTCCAAAGCC AGGTGAAGGT TTTGGATGGA TTTCTTGATG CTGATACAAA GTTTTCAGAA 
AACAGGTGCC AGAAAGCTCT GCCACTGGCT CACAGCGCCT ACCAGTCTAA CCTTCCACAC 
AACTACACCA CAACAGTACA CAAAAACCAG CTGGCTCAGG CCCTGCGGGT GTACAGCCAG 
CACGCCCGAG GCGTGGCTTT CCAGCGGTAT GCCCTGCAGC TCCACGAGGA CTGTTACAAG 
TTCTGGAGCA ATGGCCACCA GCTGTGTGAA GAGAGAAGTC TCACAGACCA GCACTGTGTT 
CACAAGTTCC ACCTCCTGCC TCAACCTGGA GAGAAAGCAG ACCTAGATCA CAACCCACCC 
ATCATGAACC ACAACAGCAG GGGGCGCTCC ACCAGCTCCT GTAACTGTGG GCGGAAACAG 
GCTCCTCGGG AGGATCCCTT TGACATCCAG TCTGCAAATT ACGACTTCTA CCAGATGCTG 
GAGGAGAAGT GCTGTGGGAA GCTGGAGAGG ATCGAGTTTC CTGTGTTCCA GCCCAGCACT 
CCGGACCCAG CGCCAGCCAG CAACCAGACT CAGCGGCTCC CATCTGAAAC AGACACAGAG 
AGGCAGAAGG AAGCGAGCAC AGCTCAGAGC CACACACCTG GAGAAACCAG TCTCAGCCTG 
GCTCTCAGTC TGGGTCAGTC CACAGACAGC CTGGAGCCCT ACGGGGATGG AGACGCACCT 
GAAAACCAGG TTCAGCCCAA GAGGCCTAGC CTCGTGGACC GTCAGCCCTC CACTGTGGAG 
TACTTACCAG GTATGATGCA CTCCAGCTGT CCAAAAGGCC TTCTCCCAAA GTTCTCCAGC 
TGGTCACTGA TCAAACTGGG CCAAGCGAAG TCTTACAACT GCCACACTGG TCTGGAACAG 
CCAGGATTCC TCCCCGGCTC TTCCTTCCTC TTGCCGTGGG ACTTGGTGAT CCGGTCTCGT 
TCGGTGGAGG ACGCAGGAGT GATGGAGCCT TTGGACGGAG GCGCGTCATC ATGGCCAGCT 
CCCAACAAGA GCCTGGTTGG AAAGCGAGGC AGCACCGGTG GGCTCGGTAG GAGTCGCAGG 
AGGGACGACA TGGCTCGGGT CTTTGTAGGG TTTGAGTACG AGGACAGCAG AGGACGACGC 
TTCATTAGCT GTGGGCCTGA TAAGATAGTA AAGGCTCTGG GGCCAGGGGG CGCCAAAGAT 
CCCGCCACCC GAGTCTTAAA CACAGACATG CCGCTCTACG TCCCATCTCC ATCACAGGGG 
CGAGGCCTGA AGCCGCACTT CGCACAGCTG ACTCGTCTCT TTATTGTGGT TCCCGACACG 
CCGCTGGAGA TCACGCTGAA CCCCCAGGTC CAACCCGGAC TTCCTCCTTG CCCCATCTTT 
CACCCGGAGC AGCCGGAGGT TGTGTTGCCC CCCGACAGCT TCTGGGTTCT GCGGTTTCCT 
TATTCCTACG CGACTGAGCG CGGCCCCTTG TTCCCCCCCA AAGAGAACCA ACCCCTCAAC 
AACTACAAGG TGCTGCGAGG CATCCTGAGA GCCTCCACAG CCAACCCTCC GCCCCAGTGA