twist cDNA ORF clone, Saccoglossus kowalevskii

Gene
Clones
gRNAs

Summary

Gene Symbol	twist
Entrez Gene ID	100303576
Full Name	twist protein
Gene Type	protein-coding
Organism	Saccoglossus kowalevskii

mRNA and Protein(s)

mRNA	Protein	Name
NM_001165012.1	NP_001158484.1	twist protein

Pathways from BioSystems
Gene Ontology

The following twist gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twist cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSg18507	NM_001165012.1 Latest version!	Saccoglossus kowalevskii twist protein (twist), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twist ( NM_001165012.1 ) cDNA ORF clone, Saccoglossus kowalevskii -> NP_001158484.1 Saccoglossus kowalevskii twist protein (twist), mRNA.

Price & Availability↑

CloneID	OSg18507
Clone ID Related Accession (Same CDS sequence)	NM_001165012.1
Accession Version	NM_001165012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Saccoglossus kowalevskii
Product	twist protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU939749.1. ##Evidence-Data-START## Transcript exon combination :: EU939749.1, FF669980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SRS543992, SRS543998 [ECO:0000348] ##Evidence-Data-END##

ATGTGTACCA CGTCGTACTT ACCGGGCCAG TTGCTCCCCA TCATGGAAGT GATGTCCACA 
TTACACCACA GCAGCTCTAT TGAGGACAGT GATAGTAGTT TTAACAGTAG TTTGAACGCA 
TCACCACCGC CCCATGATAT GGGTAGCGAA GACAACGCGG ACTATTTCAA AGAGAAGCCG 
CGTTACACGC GGAAGAGGCG ATATCGGAAG TCGGGAGATG ATAGCGAACA TGGGGGTAAA 
ATGCGTCGAA AGCACGTGAC TGCACAGACC TACGAAGATC TTCAGAACCA GCGAGTCATG 
GCTAACGTGC GGGAGAGGCA ACGGACTCAG TCTCTGAACG AAGCATTTTC AGCACTCAGA 
AAAATCATTC CAACTTTGCC ATCGGACAAA CTGAGCAAAA TACAGACTCT GAAATTGGCA 
ACAAGATACA TAGACTTTTT GTATCAAGTG CTTCGAAGTG ATGAGGTCGA CACACGGCTA 
AACATGCCGA CCAGTTGTAG TTACGTAGCA CACGAGAGAC TAAGTTATGC CTTCTCGGTG 
TGGAGGATGG AAGGTGCGTG GAATATGGCC GGTAACAGCT CGCCCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158484.1
CDS	47..637
Translation	MCTTSYLPGQLLPIMEVMSTLHHSSSIEDSDSSFNSSLNASPPPHDMGSEDNADYFKEKPRYTRKRRYRKSGDDSEHGGKMRRKHVTAQTYEDLQNQRVMANVRERQRTQSLNEAFSALRKIIPTLPSDKLSKIQTLKLATRYIDFLYQVLRSDEVDTRLNMPTSCSYVAHERLSYAFSVWRMEGAWNMAGNSSPR

Target ORF information:

RefSeq Version	NM_001165012.1
Organism	Saccoglossus kowalevskii
Definition	Saccoglossus kowalevskii twist protein (twist), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165012.1

ORF Insert Sequence:

ATGTGTACCA CGTCGTACTT ACCGGGCCAG TTGCTCCCCA TCATGGAAGT GATGTCCACA 
TTACACCACA GCAGCTCTAT TGAGGACAGT GATAGTAGTT TTAACAGTAG TTTGAACGCA 
TCACCACCGC CCCATGATAT GGGTAGCGAA GACAACGCGG ACTATTTCAA AGAGAAGCCG 
CGTTACACGC GGAAGAGGCG ATATCGGAAG TCGGGAGATG ATAGCGAACA TGGGGGTAAA 
ATGCGTCGAA AGCACGTGAC TGCACAGACC TACGAAGATC TTCAGAACCA GCGAGTCATG 
GCTAACGTGC GGGAGAGGCA ACGGACTCAG TCTCTGAACG AAGCATTTTC AGCACTCAGA 
AAAATCATTC CAACTTTGCC ATCGGACAAA CTGAGCAAAA TACAGACTCT GAAATTGGCA 
ACAAGATACA TAGACTTTTT GTATCAAGTG CTTCGAAGTG ATGAGGTCGA CACACGGCTA 
AACATGCCGA CCAGTTGTAG TTACGTAGCA CACGAGAGAC TAAGTTATGC CTTCTCGGTG 
TGGAGGATGG AAGGTGCGTG GAATATGGCC GGTAACAGCT CGCCCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.