Recombinant CHO-K1 cells stably overexpress human TNF receptor superfamily member 9 (4-1BB) on the surface. The surface expression of 4-1BB is validated by FACS analysis.

human

OX40 (CD134) is a member of the TNFR/TNF superfamily. Costimulatory signals from OX40 to a conventional T cell promotes division and survival, augmenting the clonal expansion of effector and memory populations as they are being generated to antigens. OX40 additionally suppresses the differentiation and activity of Treg, further amplifying this process.

Human

Recombinant CHO-K1 cells stably overexpress Homo sapiens TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against human HVEM or to measure binding affinity between HVEM and anti-HVEM antibodies.

Human

Recombinant CHO-K1 cells stably overexpress Homo sapiens TNF receptor superfamily member 18 (GITR) on the surface. The surface expression of GITR is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against human GITR or to measure binding affinity between GITR and anti-GITR antibodies.

Mouse

Recombinant CHO-K1 cells stably overexpress Mus musculus TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against mouse HVEM or to measure binding affinity between HVEM and anti-HVEM antibodies.

Cyno

Recombinant CHO-K1 cells stably overexpress Macaca fascicularis TNF receptor superfamily member 14 (HVEM) on the surface. The surface expression of HVEM is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against cyno HVEM or to measure binding affinity between HVEM and anti- HVEM antibodies.

cyno

4-1BB is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), CD137 and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule. 4-1BB can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, 4-1BB expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Cyno

HVEM is found on the surface of various cell types, including hematopoietic and non-hematopoietic cells, and is expressed mainly in spleen, thymus, bone marrow, lung and intestines. It has been identified as a canonical TNF receptor, signaling through the TNF receptor-associated factors (TRAFs) leading to NFκB activation. Furthermore, in addition to acting as a signaling receptor, in binding to Ig superfamily members it acts as a ligand for these inhibitory receptors. Therefore, bidirectional signaling is possible for the HVEM-mediated signaling network, which can be involved in positive or negative immunological reactions under different contexts.