結果： Rush mRNAのタンパク質発現レベルは標準mRNAと比較して同等であり、下流アプリケーションが担保された製品をより早く納品することができます。
mRNA技術は急速に進歩しており、効率的なmRNA産生の需要が高まっています。ジェンスクリプトは、お客様の創薬やターゲットスクリーニングの迅速化や、mRNAライブラリー構築の効率化などのために設計された、カスタムRush mRNAサービスをご提供しています。mRNA製品の品質を損なうことなく、プロジェクトのスケジュールを最大50%短縮できます。今すぐお試しください。
２週間
遺伝子合成からmRNA
製造の期間を短縮
＞98% Capping
ハイパフォーマンスmRNA
固定価格制
速やかなお見積り
発見から前臨床mRNA生産まで
5' Cap Options
Poly(A) Tail Option
Modified NTP options
QC
5' Cap Options
Poly(A) Tail Option
Modified NTP options
QC
結果： Rush mRNAのタンパク質発現レベルは標準mRNAと比較して同等であり、下流アプリケーションが担保された製品をより早く納品することができます。
新規2’-O-Ethyl-capped RNAは、天然のCap1 RNAよりも高い発現を示し、生体内でのクリアランス速度が遅いことが確認されました。
ラボ
信頼される
納品成功
プロジェクト
Dr. Sean
Whelan
Washington University School of Medicine
"Contracting with Genscript for the production of mRNA-LNPs has advanced my
laboratory's research into viral vaccines by allowing us to rapidly produce and test
multiple mRNA vaccine constructs for several different viruses.
"
Chun-YuChen
Seattle Children's Hospital
"I purchased mRNA from different companies and found that GenScript's mRNA showed
higher expression (almost 2 fold) and much more cost effective!!"
We use linearized DNA plasmid as a template for manufacturing your mRNA. The gene can be designed by our team using GenScript’s proprietary vectors and tools or we can use one of your designs. Once the design has been agreed to, we handle the gene and mRNA manufacture and provide you with mRNA as part of the fee-for-service agreement.
Cap1 via co-transcriptional capping is our default recommendation. We can also offer Cap0 and Cap1 enzymatically for an additional cost, and Cap2 RNA through co-transcriptional capping technology.
GenScript's proprietary capping technology can achieve >98% capping efficiency.
mRNA should be stable for at least 1 year if stored properly at -80°C upon receival and avoiding repeated freezing and thawing cycles, however mRNA stability varies depending on the length of mRNA. We have tested mRNA with stable results up to 3 freeze/thaw cycles. We recommend storing at -80°C for long time storage and -20°C for short term storage.
We can make mRNA from 100bp to 20kb in length. For oligo shorter than 100bp, our oligo synthesis team can take it under chemical syntehsis.
For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification. It is suitable for in vitro and invivo proof of concept studies.
For preclinical grade, we use HPLC based Oligo dT purification for improving RNA purity. Preclinical grade can be considered for long mRNA constructs >5kb or when high RNA purity (less fragment RNA ) is required for the project.
We can provide cGMP mRNA through our sister company ProBio. Please contact our technical support team.
Yes. We guarantee specific amounts of mRNA be delivered.
We ship mRNA at concentration of 0.5 ~2mg/mL by default. We can adjust concentration to a specific value <4mg /mL per customer's request at extra cost.
We offer 3 options of buffer: 1mM sodium citrate pH 6.5, TE pH7, and RNase-Free H2O. 1mM sodium citrate is our default option for better mRNA stability.
For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification.
For preclinical grade, we use HPLC-based Oligo dT purification for improving RNA purity.
In addition, we offer optional dephosphorylation to further reduce triphosphate RNA species in the final product to reduce its immunogenicity, and optional dsRNA removal HPLC purification to provide higher quality mRNA if low dsRNA residue is required.
Cellulose based HPLC method.
For size-based purity, we run the mRNA sample on bioanalyzer, then calculate the ratio of the main peak (target size +-15%) over the total AUC.
We recommend customers dephosphorylation as reducing the triphosphate-RNA species in mRNA by dephosphorylation could reduce its immunogenicity, thus making the transfected cells healthier and can express the target protein better.
Modifications help stabilize mRNA and reduce its immunogenicity.
We can substitute uridine bases with either 5-moU, N1-me-Ψ, Ψ, 5’-iodourdine, or Cy5-UTP.
We can substitute cytidine bases with 5-me-C, 5’-iodocytidine, or thiol-CTP.
We can substitute customizable percentage substitutions upon request. These will be not site specific modifications.
Capping can stablize RNA from 5'end and recruit ribosome. Cap1 and Cap 2 have a higher impact on mRNA stability compared to Cap 0 regarding protecting the mRNA from exonuclease degradation. It is common to use Cap1 for mammalian cells and Cap 0 for plant cells. Cap2 is a novel solution provided by GenScript that may work better than Cap1 in mammailan cells especiailly in immune-stressed conditions.
Yes, we offer mRNA QC service including capping efficiency assay using enzyme digestion /LC-MS method. Please contact our technical support team for details.
Yes, it is possible, please contact our technical support team.
For making cap1 mRNA using co-transcriptional capping technology, either AGG or GGG following T7 promoter seq is required. For self-amplifying RNA usually ATG after T7 promoter.
Yes, we can do codon optimization. Please contact our technical support team.
Yes. we can subclone your ORF sequences into our proprietary vector which contains T7 promoter, UTR and 100A for mRNA production.
We use RNaseT1.
Yes, please refer to https://www.genscript.com/mrna-qc-services.html.
Approximately 0.5ug to 1 ug of mRNA is usually used for transfecting each well in 24 well plate.
Questions On Storage And Usage Guidelines_mRNA, saRNA and LNP
CRISPR Knock-in Protocol for HEK 293T/293 cells with Thermo Fisher Neon® Transfection System
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