Loaded VEGF165, His & Avi, Human, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay (Gator® Prime).

Loaded VEGF165, His & Avi, Human, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay (Gator® Prime).

Loaded VEGF165, His & Avi, Human, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay (Gator® Prime).

Immobilized VEGF165, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 23.5 ng/ml determined by ELISA.

Immobilized VEGF165, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.5 ng/ml determined by ELISA.

Immobilized VEGF165, His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.

VEGF165, His & Avi, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

VEGF165, His & Avi, Human

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Cat. No.	Z05953
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
TAT
Price	¥69,021.00


Ask us a question

Product Introduction

Species

Human

Protein Construction

VEGF165 (Ala27-Arg191)_x000D_ Accession # P15692-4	His	Avi
N-term		C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

22.2 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples