SIRP alpha V2/CD172a hFc Chimera, Human
SIRP Alpha V2/CD172a HFc Chimera, Human

Immobilized Human CD47, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for SIRP alpha V2/CD172a hFc Chimera, Human, hFc Tag with the EC50 of 41.9ng/ml determined by ELISA.

SIRP Alpha V2/CD172a HFc Chimera, Human

Immobilized SIRP alpha V2/CD172a hFc Chimera, Human, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 33.9ng/ml determined by ELISA.

SIRP Alpha V2/CD172a HFc Chimera, Human

The purity of SIRP alpha V2/CD172a hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

SIRP Alpha V2/CD172a HFc Chimera, Human

SIRP alpha V2/CD172a hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SIRP alpha V2/CD172a hFc Chimera, Human

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
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Product Introduction
Species Human
Protein Construction
SIRP alpha V2/CD172a (Glu31-Arg369)
Accession # AAH26692.1		 hFc
N-term C-term
Purity > 95% as determined by Bis-­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 63.9 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 85-95 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • SIRP Alpha V2/CD172a HFc Chimera, Human
    • SIRP Alpha V2/CD172a HFc Chimera, Human

    Immobilized Human CD47, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for SIRP alpha V2/CD172a hFc Chimera, Human, hFc Tag with the EC50 of 41.9ng/ml determined by ELISA.

  • SIRP Alpha V2/CD172a HFc Chimera, Human
    • SIRP Alpha V2/CD172a HFc Chimera, Human

    Immobilized SIRP alpha V2/CD172a hFc Chimera, Human, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 33.9ng/ml determined by ELISA.

  • SIRP Alpha V2/CD172a HFc Chimera, Human
    • SIRP Alpha V2/CD172a HFc Chimera, Human

    The purity of SIRP alpha V2/CD172a hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • SIRP Alpha V2/CD172a HFc Chimera, Human
    • SIRP Alpha V2/CD172a HFc Chimera, Human

    SIRP alpha V2/CD172a hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the 'don´t eat me' signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Synonyms CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA; SIRPalpha2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.