RNF43 hFc Chimera, Human
RNF43 HFc Chimera, Human

Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind RNF43 hFc Chimera, Human, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime).

RNF43 HFc Chimera, Human

Immobilized Human R-Spondin 3, His Tag at 2 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.

RNF43 HFc Chimera, Human

Immobilized Human R spondin 1 at 5 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 0.4 μg /ml determined by ELISA.

RNF43 HFc Chimera, Human

The purity of RNF43 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

RNF43 HFc Chimera, Human

RNF43 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

RNF43 hFc Chimera, Human

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Z05791
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Product Introduction
Species Human
Protein Construction
RNF43 (Gly24-Tyr197)
Accession # Q68DV7-1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 45.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • RNF43 HFc Chimera, Human
    • RNF43 HFc Chimera, Human

    Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind RNF43 hFc Chimera, Human, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime).

  • RNF43 HFc Chimera, Human
    • RNF43 HFc Chimera, Human

    Immobilized Human R-Spondin 3, His Tag at 2 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.

  • RNF43 HFc Chimera, Human
    • RNF43 HFc Chimera, Human

    Immobilized Human R spondin 1 at 5 μg /ml(100 μl/well) on the plate. Dose response curve for RNF43 hFc Chimera, Human, hFc Tag with the EC50 of 0.4 μg /ml determined by ELISA.

  • RNF43 HFc Chimera, Human
    • RNF43 HFc Chimera, Human

    The purity of RNF43 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • RNF43 HFc Chimera, Human
    • RNF43 HFc Chimera, Human

    RNF43 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Synonyms E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.