R spondin 1/RSPO1, His, Human
R Spondin 1/RSPO1, His, Human

Loaded R spondin 1/RSPO1, His, Human, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime).

R Spondin 1/RSPO1, His, Human

Loaded R spondin 1/RSPO1, His, Human, His Tag on Anti-His-Biosensor can bind Human LRP-6, mFc Tag with an affinity constant of 8.97 nM as determined in BLI assay (Gator® Prime).

R Spondin 1/RSPO1, His, Human

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

R Spondin 1/RSPO1, His, Human

Immobilized R spondin 1/RSPO1, His, Human, His Tag at 0.5 μg/ml (100 μI/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

R Spondin 1/RSPO1, His, Human

Immobilized Human LRP-6, mFc Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for R spondin 1/RSPO1, His, Human, His Tag with the EC50 of 4.4 μg/ml determined by ELISA.

R Spondin 1/RSPO1, His, Human

Immobilized R spondin 1/RSPO1, His, Human at 5 μg/ml(100 μI/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4 μg/ml determined by ELISA.

R Spondin 1/RSPO1, His, Human

R spondin 1/RSPO1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

R spondin 1/RSPO1, His, Human

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
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Product Introduction
Species Human
Protein Construction
R spondin 1/RSPO1 (Arg31-Ala263)_x000D_
Accession # Q2MKA7		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 0.05EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System CHO
Theoretical Molecular Weight 27.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Loaded R spondin 1/RSPO1, His, Human, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay (Gator® Prime).

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Loaded R spondin 1/RSPO1, His, Human, His Tag on Anti-His-Biosensor can bind Human LRP-6, mFc Tag with an affinity constant of 8.97 nM as determined in BLI assay (Gator® Prime).

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Immobilized R spondin 1/RSPO1, His, Human, His Tag at 0.5 μg/ml (100 μI/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Immobilized Human LRP-6, mFc Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for R spondin 1/RSPO1, His, Human, His Tag with the EC50 of 4.4 μg/ml determined by ELISA.

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    Immobilized R spondin 1/RSPO1, His, Human at 5 μg/ml(100 μI/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4 μg/ml determined by ELISA.

  • R Spondin 1/RSPO1, His, Human
    • R Spondin 1/RSPO1, His, Human

    R spondin 1/RSPO1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.