Immobilized MICB, His & Avi, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA.

Immobilized MICB, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.

The purity of MICB, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

MICB, His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind MICB, His & Avi, Human, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).

MICB, His & Avi, Human

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Cat. No.	Z05651
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥72,752.00


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Product Introduction

Species

Human

Protein Construction

MICB (Ala23-Gly298)_x000D_ Accession # Q29980-1	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

34.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized MICB, His & Avi, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA.

Immobilized MICB, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.

The purity of MICB, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

MICB, His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind MICB, His & Avi, Human, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).

Background

Target Background	MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Synonyms	MICB; MIC-B; PERB11.2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

MICB, His & Avi, Human

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