LIGHT/TNFSF14 hFc Chimera, Human
LIGHT/TNFSF14 HFc Chimera, Human

Immobilized Human HVEM, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for LIGHT/TNFSF14 hFc Chimera, Human, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.

LIGHT/TNFSF14 HFc Chimera, Human

The purity of LIGHT/TNFSF14 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

LIGHT/TNFSF14 HFc Chimera, Human

LIGHT/TNFSF14 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

LIGHT/TNFSF14 hFc Chimera, Human

LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Z05582
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Product Introduction
Species Human
Protein Construction
hFc LIGHT/TNFSF14 (Asp74-Val240)
Accession # O43557-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 45.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • LIGHT/TNFSF14 HFc Chimera, Human
    • LIGHT/TNFSF14 HFc Chimera, Human

    Immobilized Human HVEM, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for LIGHT/TNFSF14 hFc Chimera, Human, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.

  • LIGHT/TNFSF14 HFc Chimera, Human
    • LIGHT/TNFSF14 HFc Chimera, Human

    The purity of LIGHT/TNFSF14 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • LIGHT/TNFSF14 HFc Chimera, Human
    • LIGHT/TNFSF14 HFc Chimera, Human

    LIGHT/TNFSF14 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Synonyms CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.