E-Selectin/CD62E, His & Avi, Human
E-Selectin/CD62E, His & Avi, Human

Immobilized E-Selectin/CD62E, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.

E-Selectin/CD62E, His & Avi, Human

The purity of E-Selectin/CD62E, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

E-Selectin/CD62E, His & Avi, Human

E-Selectin/CD62E, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

E-Selectin/CD62E, His & Avi, Human

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Z05265
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Product Introduction
Species Human
Protein Construction
E-Selectin/CD62E (Trp22-Pro556)
Accession # P16581		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 61.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • E-Selectin/CD62E, His & Avi, Human
    • E-Selectin/CD62E, His & Avi, Human

    Immobilized E-Selectin/CD62E, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.

  • E-Selectin/CD62E, His & Avi, Human
    • E-Selectin/CD62E, His & Avi, Human

    The purity of E-Selectin/CD62E, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • E-Selectin/CD62E, His & Avi, Human
    • E-Selectin/CD62E, His & Avi, Human

    E-Selectin/CD62E, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Synonyms CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.