HGF R/c-MET, His, Cynomolgus
HGF R/c-MET, His, Cynomolgus

Loaded Anti-HGF R Antibody, hFc Tag on ProA-Biosensor can bind HGF R/c-MET, His, Cynomolgus, His Tag with an affinity constant of 2.15 nM as determined in BLI assay (Gator® Prime).

HGF R/c-MET, His, Cynomolgus

Immobilized HGF R/c-MET, His, Cynomolgus, His Tag at 5 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.

HGF R/c-MET, His, Cynomolgus

The purity of HGF R/c-MET, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

HGF R/c-MET, His, Cynomolgus

HGF R/c-MET, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

HGF R/c-MET, His, Cynomolgus

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Z04807
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Product Introduction
Species Cynomolgus
Protein Construction
HGF R/c-MET (Glu25-Asn930)
Accession # A0A2K5UM36		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 32.5 kDa (α chain) and 69.9 kDa (β chain)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.2).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • HGF R/c-MET, His, Cynomolgus
    • HGF R/c-MET, His, Cynomolgus

    Loaded Anti-HGF R Antibody, hFc Tag on ProA-Biosensor can bind HGF R/c-MET, His, Cynomolgus, His Tag with an affinity constant of 2.15 nM as determined in BLI assay (Gator® Prime).

  • HGF R/c-MET, His, Cynomolgus
    • HGF R/c-MET, His, Cynomolgus

    Immobilized HGF R/c-MET, His, Cynomolgus, His Tag at 5 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.

  • HGF R/c-MET, His, Cynomolgus
    • HGF R/c-MET, His, Cynomolgus

    The purity of HGF R/c-MET, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

  • HGF R/c-MET, His, Cynomolgus
    • HGF R/c-MET, His, Cynomolgus

    HGF R/c-MET, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.