ULBP-2[Biotin], His & Avi, Human
ULBP-2[Biotin], His & Avi, Human

Serial dilutions of Anti- NKG2D Antibody were added into ULBP-2[Biotin], His & Avi, Human, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 45.5ng/ml.

ULBP-2[Biotin], His & Avi, Human

Immobilized ULBP-2[Biotin], His & Avi, Human, His Avi Tag at 2μg/ml(100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30μg/ml determined by ELISA.

ULBP-2[Biotin], His & Avi, Human

The purity of ULBP-2[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

ULBP-2[Biotin], His & Avi, Human

ULBP-2[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ULBP-2[Biotin], His & Avi, Human

ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated 'mature' NK cells.
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Product Introduction
Species Human
Protein Construction
ULBP-2 (Gly26-Ser217)
Accession # Q9BZM5		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 24.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • ULBP-2[Biotin], His & Avi, Human
    • ULBP-2[Biotin], His & Avi, Human

    Serial dilutions of Anti- NKG2D Antibody were added into ULBP-2[Biotin], His & Avi, Human, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 45.5ng/ml.

  • ULBP-2[Biotin], His & Avi, Human
    • ULBP-2[Biotin], His & Avi, Human

    Immobilized ULBP-2[Biotin], His & Avi, Human, His Avi Tag at 2μg/ml(100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30μg/ml determined by ELISA.

  • ULBP-2[Biotin], His & Avi, Human
    • ULBP-2[Biotin], His & Avi, Human

    The purity of ULBP-2[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • ULBP-2[Biotin], His & Avi, Human
    • ULBP-2[Biotin], His & Avi, Human

    ULBP-2[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated 'mature' NK cells.
Synonyms ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; ULBP2; ULBP-2/5/6; NKG2D ligand 2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.