TIGIT[Biotin], His & Avi, Human
TIGIT[Biotin], His & Avi, Human

Immobilized TIGIT[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

TIGIT[Biotin], His & Avi, Human

The purity of TIGIT[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

TIGIT[Biotin], His & Avi, Human

TIGIT[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

TIGIT[Biotin], His & Avi, Human

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
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Product Introduction
Species Human
Protein Construction
TIGIT (Met22-Pro141)
Accession # Q495A1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • TIGIT[Biotin], His & Avi, Human
    • TIGIT[Biotin], His & Avi, Human

    Immobilized TIGIT[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

  • TIGIT[Biotin], His & Avi, Human
    • TIGIT[Biotin], His & Avi, Human

    The purity of TIGIT[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • TIGIT[Biotin], His & Avi, Human
    • TIGIT[Biotin], His & Avi, Human

    TIGIT[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Synonyms TIGIT; VSIG9; VSTM3; WUCAM

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.