PD-L1/B7-H1[Biotin], His & Avi, Human
PD-L1/B7-H1[Biotin], His & Avi, Human

Immobilized PD-L1/B7-H1[Biotin], His & Avi, Human, His-Avi Tag at 1μg/ml (100μl/Well) on streptavidin(5mg/ml) precoated plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

PD-L1/B7-H1[Biotin], His & Avi, Human

Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for PD-L1/B7-H1[Biotin], His & Avi, Human, His Tag with the EC50 of 9.4ng/ml determined by ELISA.

PD-L1/B7-H1[Biotin], His & Avi, Human

The purity of PD-L1/B7-H1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

PD-L1/B7-H1[Biotin], His & Avi, Human

PD-L1/B7-H1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

PD-L1/B7-H1[Biotin], His & Avi, Human

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Z04585
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Product Introduction
Species Human
Protein Construction
PD-L1/B7-H1 (Phe19-Arg238)
Accession # Q9NZQ7-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • PD-L1/B7-H1[Biotin], His & Avi, Human
    • PD-L1/B7-H1[Biotin], His & Avi, Human

    Immobilized PD-L1/B7-H1[Biotin], His & Avi, Human, His-Avi Tag at 1μg/ml (100μl/Well) on streptavidin(5mg/ml) precoated plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

  • PD-L1/B7-H1[Biotin], His & Avi, Human
    • PD-L1/B7-H1[Biotin], His & Avi, Human

    Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for PD-L1/B7-H1[Biotin], His & Avi, Human, His Tag with the EC50 of 9.4ng/ml determined by ELISA.

  • PD-L1/B7-H1[Biotin], His & Avi, Human
    • PD-L1/B7-H1[Biotin], His & Avi, Human

    The purity of PD-L1/B7-H1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • PD-L1/B7-H1[Biotin], His & Avi, Human
    • PD-L1/B7-H1[Biotin], His & Avi, Human

    PD-L1/B7-H1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.