HGF R/c-MET[Biotin], His & Avi, Human
HGF R/c-MET[Biotin], His & Avi, Human

Immobilized Anti-HGF R Antibody, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for HGF R/c-MET[Biotin], His & Avi, Human, His Tag with the EC50 of 20.8ng/ml determined by ELISA.

HGF R/c-MET[Biotin], His & Avi, Human

The purity of HGF R/c-MET[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

HGF R/c-MET[Biotin], His & Avi, Human

HGF R/c-MET[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

HGF R/c-MET[Biotin], His & Avi, Human

et, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Z04478
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Product Introduction
Species Human
Protein Construction
HGF R/c-MET (Glu25-Thr932)
Accession # P08581-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 32.5 kDa (α chain) and 72.1 kDa (β chain)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • HGF R/c-MET[Biotin], His & Avi, Human
    • HGF R/c-MET[Biotin], His & Avi, Human

    Immobilized Anti-HGF R Antibody, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for HGF R/c-MET[Biotin], His & Avi, Human, His Tag with the EC50 of 20.8ng/ml determined by ELISA.

  • HGF R/c-MET[Biotin], His & Avi, Human
    • HGF R/c-MET[Biotin], His & Avi, Human

    The purity of HGF R/c-MET[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • HGF R/c-MET[Biotin], His & Avi, Human
    • HGF R/c-MET[Biotin], His & Avi, Human

    HGF R/c-MET[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background et, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.