EpCAM/TROP1[Biotin], His & Avi, Human
EpCAM/TROP1[Biotin], His & Avi, Human

Immobilized Anti-EpCAM Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for EpCAM/TROP1[Biotin], His & Avi, Human, His Tag with the EC50 of 21.2 ng/ml determined by ELISA.

EpCAM/TROP1[Biotin], His & Avi, Human

The purity of EpCAM/TROP1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

EpCAM/TROP1[Biotin], His & Avi, Human

EpCAM/TROP1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

EpCAM/TROP1[Biotin], His & Avi, Human

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
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Product Introduction
Species Human
Protein Construction
EpCAM/TROP1 (Gln24-Lys265)
Accession # P16422		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 30.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • EpCAM/TROP1[Biotin], His & Avi, Human
    • EpCAM/TROP1[Biotin], His & Avi, Human

    Immobilized Anti-EpCAM Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for EpCAM/TROP1[Biotin], His & Avi, Human, His Tag with the EC50 of 21.2 ng/ml determined by ELISA.

  • EpCAM/TROP1[Biotin], His & Avi, Human
    • EpCAM/TROP1[Biotin], His & Avi, Human

    The purity of EpCAM/TROP1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • EpCAM/TROP1[Biotin], His & Avi, Human
    • EpCAM/TROP1[Biotin], His & Avi, Human

    EpCAM/TROP1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Synonyms CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.