DNAM-1/CD226[Biotin], His & Avi, Human
DNAM-1/CD226[Biotin], His & Avi, Human

Immobilized DNAM-1/CD226[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 8.6 ng/ml determined by ELISA.

DNAM-1/CD226[Biotin], His & Avi, Human

The purity of DNAM-1/CD226[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

DNAM-1/CD226[Biotin], His & Avi, Human

DNAM-1/CD226[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

DNAM-1/CD226[Biotin], His & Avi, Human

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind DNAM-1/CD226[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).

DNAM-1/CD226[Biotin], His & Avi, Human

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
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Product Introduction
Species Human
Protein Construction
DNAM-1/CD226 (Glu19--Asn247)
Accession # Q15762		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.9 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • DNAM-1/CD226[Biotin], His & Avi, Human
    • DNAM-1/CD226[Biotin], His & Avi, Human

    Immobilized DNAM-1/CD226[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 8.6 ng/ml determined by ELISA.

  • DNAM-1/CD226[Biotin], His & Avi, Human
    • DNAM-1/CD226[Biotin], His & Avi, Human

    The purity of DNAM-1/CD226[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • DNAM-1/CD226[Biotin], His & Avi, Human
    • DNAM-1/CD226[Biotin], His & Avi, Human

    DNAM-1/CD226[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • DNAM-1/CD226[Biotin], His & Avi, Human
    • DNAM-1/CD226[Biotin], His & Avi, Human

    Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind DNAM-1/CD226[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).


Background
Target Background DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.