DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human
DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

Immobilized Human LRP-6, mFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 2.27 μg/ml determined by ELISA.

DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

Immobilized Anti-DKK1 Antibody at 1 μg/ml(100 μl/well) on the plate. Dose response curve for DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.

DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Z04438
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Product Introduction
Species Human
Protein Construction
DKK1 C terminal Domain (Met178-His266)
Accession # O94907		 hFc Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 38.69 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0).
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human
    • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

    Immobilized Human LRP-6, mFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 2.27 μg/ml determined by ELISA.

  • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human
    • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

    DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human
    • DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human

    Immobilized Anti-DKK1 Antibody at 1 μg/ml(100 μl/well) on the plate. Dose response curve for DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.


Background
Target Background Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Synonyms Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.